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Protein

Pre-hexon-linking protein IIIa

Gene

L1

Organism
Human adenovirus C serotype 2 (HAdV-2) (Human adenovirus 2)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Structural component of the virion that acts as a cement protein on the capsid exterior which mediates the interactions between the hexons, including the peripentonal hexons, and reaches all the way to the penton vertices. Two hexon linking proteins IIIa, one from each facet, stabilize the unique edge interface between a pair of facets. As the virus enters the host cell, hexon linking proteins IIIa are shed concomitant with virion acidification in the endosome. During virus assembly, seems to play a role in the serotype specificity of the packaging of viral DNA via its interaction with packaging protein 3.UniRule annotation

Miscellaneous

All late proteins expressed from the major late promoter are produced by alternative splicing and alternative polyadenylation of the same gene giving rise to non-overlapping ORFs. A leader sequence is present in the N-terminus of all these mRNAs and is recognized by the viral shutoff protein to provide expression although conventional translation via ribosome scanning from the cap has been shut off in the host cell.UniRule annotation

Keywordsi

Biological processViral genome packaging, Viral release from host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-hexon-linking protein IIIaUniRule annotation
Alternative name(s):
Capsid vertex-specific component IIIaUniRule annotation
Short name:
CVSCUniRule annotation
Protein IIIaUniRule annotation
pIIIaUniRule annotation
Cleaved into the following chain:
Hexon-linking protein IIIaUniRule annotation
Gene namesi
ORF Names:L1
OrganismiHuman adenovirus C serotype 2 (HAdV-2) (Human adenovirus 2)
Taxonomic identifieri10515 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAdenoviridaeMastadenovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000008167 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Capsid decoration protein, Capsid protein, Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004210731 – 585Pre-hexon-linking protein IIIaUniRule annotationAdd BLAST585
ChainiPRO_00002218341 – 570Hexon-linking protein IIIaUniRule annotationAdd BLAST570
PropeptideiPRO_0000421074571 – 585UniRule annotation1 PublicationAdd BLAST15

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei225Phosphoserine; by hostUniRule annotation1 Publication1
Modified residuei274Phosphothreonine; by hostUniRule annotation1 Publication1
Modified residuei310Phosphoserine; by hostUniRule annotation1 Publication1
Modified residuei444Phosphoserine; by hostUniRule annotation1 Publication1
Modified residuei449Phosphoserine; by hostUniRule annotation1 Publication1
Modified residuei450Phosphoserine; by hostUniRule annotation1 Publication1
Modified residuei452Phosphoserine; by hostUniRule annotation1 Publication1
Modified residuei469Phosphoserine; by hostUniRule annotation1 Publication1
Modified residuei473Phosphoserine; by hostUniRule annotation1 Publication1
Modified residuei490Phosphotyrosine; by hostUniRule annotation1 Publication1
Modified residuei494Phosphoserine; by hostUniRule annotation1 Publication1
Modified residuei515Phosphoserine; by hostUniRule annotation1 Publication1

Post-translational modificationi

Cleaved near the C-terminus by the viral protease during virion maturation to form the mature protein.UniRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei570 – 571Cleavage; by viral proteaseUniRule annotation1 Publication2

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP03279

PTM databases

iPTMnetiP03279

Expressioni

Inductioni

Expressed in the late phase of the viral replicative cycle.UniRule annotation

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Interacts with hexon proteins; this interaction tethers the peripentonal hexons to hexons situated in the facet. Interacts with the penton protein (via N-terminus). Interacts with packaging protein 3; this interaction is required to promote correct genome packaging.UniRule annotationBy similarity

Structurei

3D structure databases

SMRiP03279
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 106Peripentonal hexon-tethering domainUniRule annotationAdd BLAST106
Regioni138 – 251Binding to hexon-linking proteinUniRule annotationAdd BLAST114

Sequence similaritiesi

Belongs to the adenoviridae hexon-linking protein IIIa family.UniRule annotationCurated

Phylogenomic databases

OrthoDBiVOG0900006A

Family and domain databases

HAMAPiMF_04047 ADV_CAP3, 1 hit
InterProiView protein in InterPro
IPR003479 Hex_IIIa
PfamiView protein in Pfam
PF02455 Hex_IIIa, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P03279-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MMQDATDPAV RAALQSQPSG LNSTDDWRQV MDRIMSLTAR NPDAFRQQPQ
60 70 80 90 100
ANRLSAILEA VVPARANPTH EKVLAIVNAL AENRAIRPDE AGLVYDALLQ
110 120 130 140 150
RVARYNSGNV QTNLDRLVGD VREAVAQRER AQQQGNLGSM VALNAFLSTQ
160 170 180 190 200
PANVPRGQED YTNFVSALRL MVTETPQSEV YQSGPDYFFQ TSRQGLQTVN
210 220 230 240 250
LSQAFKNLQG LWGVRAPTGD RATVSSLLTP NSRLLLLLIA PFTDSGSVSR
260 270 280 290 300
DTYLGHLLTL YREAIGQAHV DEHTFQEITS VSRALGQEDT GSLEATLNYL
310 320 330 340 350
LTNRRQKIPS LHSLNSEEER ILRYVQQSVS LNLMRDGVTP SVALDMTARN
360 370 380 390 400
MEPGMYASNR PFINRLMDYL HRAAAVNPEY FTNAILNPHW LPPPGFYTGG
410 420 430 440 450
FEVPEGNDGF LWDDIDDSVF SPQPQTLLEL QQREQAEAAL RKESFRRPSS
460 470 480 490 500
LSDLGAAAPR SDASSPFPSL IGSFTSTRTT RPRLLGEEEY LNNSLLQPQR
510 520 530 540 550
EKNLPPAFPN NGIESLVDKM SRWKTYAQEH RDVPGPRPPT RRQRHDRQRG
560 570 580
LVWEDDDSAD DSSVLDLGGS GNPFAHLRPR LGRMF
Length:585
Mass (Da):65,287
Last modified:July 21, 1986 - v1
Checksum:i75D7C8053A91E475
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01917 Genomic DNA Translation: AAA92210.1
PIRiA03851 SXAD32
RefSeqiAP_000169.1, AC_000007.1
NP_040520.1, NC_001405.1

Genome annotation databases

GeneIDi2652993
KEGGivg:2652993

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01917 Genomic DNA Translation: AAA92210.1
PIRiA03851 SXAD32
RefSeqiAP_000169.1, AC_000007.1
NP_040520.1, NC_001405.1

3D structure databases

SMRiP03279
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiP03279

Proteomic databases

PRIDEiP03279

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2652993
KEGGivg:2652993

Phylogenomic databases

OrthoDBiVOG0900006A

Family and domain databases

HAMAPiMF_04047 ADV_CAP3, 1 hit
InterProiView protein in InterPro
IPR003479 Hex_IIIa
PfamiView protein in Pfam
PF02455 Hex_IIIa, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCAP3_ADE02
AccessioniPrimary (citable) accession number: P03279
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: June 20, 2018
This is version 65 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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