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Protein

Penton protein

Gene

L2

Organism
Human adenovirus C serotype 2 (HAdV-2) (Human adenovirus 2)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Major capsid protein that self-associates to form penton base pentamers, each in the shape of a pentagon, situated at the 12 vertices of the pseudo T=25 capsid. Involved in virus secondary attachment to host cell after initial attachment by the fiber protein. Binds host integrin heterodimer ITGAV-ITGB5 (alphaV-beta5) thereby triggering clathrin-mediated endocytosis of virions. Mediates initial virus attachment to CXADR-negative cells. Binding to integrins ITGAV-ITGB5 also seems to induce macropinocytosis uptake of the virus. As the virus enters the host cell, penton proteins are shed concomitant with virion acidification in the endosome.UniRule annotation3 Publications

Miscellaneous

All late proteins expressed from the major late promoter are produced by alternative splicing and alternative polyadenylation of the same gene giving rise to non-overlapping ORFs. A leader sequence is present in the N-terminus of all these mRNAs and is recognized by the viral shutoff protein to provide expression although conventional translation via ribosome scanning from the cap has been shut off in the host cell.UniRule annotation

GO - Biological processi

Keywordsi

Biological processClathrin-mediated endocytosis of virus by host, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus endocytosis by host, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Penton proteinUniRule annotation
Short name:
CP-PUniRule annotation
Alternative name(s):
Penton base proteinUniRule annotation
Protein IIIUniRule annotation
Gene namesi
Name:L2UniRule annotation
OrganismiHuman adenovirus C serotype 2 (HAdV-2) (Human adenovirus 2)
Taxonomic identifieri10515 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAdenoviridaeMastadenovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000008167 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Capsid protein, Host nucleus, T=25 icosahedral capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002218721 – 571Penton proteinAdd BLAST571

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei455Phosphoserine; by host1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP03276

PTM databases

iPTMnetiP03276

Expressioni

Inductioni

Expressed in the late phase of the viral replicative cycle.UniRule annotation

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Interacts with the fiber protein (via N-terminal tail region). Interacts with the capsid vertex protein; this interaction binds the penton base to neighboring peripentonal hexons. Interacts (via the cell attachment site RGD) with host heterodimer ITGAV-ITGB5; this interaction promotes virus internalization. Interacts with host WWP1 and WWP2.UniRule annotation4 Publications

Protein-protein interaction databases

ELMiP03276

Structurei

Secondary structure

1571
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP03276
SMRiP03276
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03276

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni336 – 348DisorderedUniRule annotationAdd BLAST13

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi340 – 342Cell attachment siteUniRule annotation3

Domaini

The cell attachment RGD motif is exposed at the virion surface and is involved in binding to the integrin heterodimer ITGAV-ITGB5.UniRule annotation

Sequence similaritiesi

Belongs to the adenoviridae penton family.UniRule annotation

Phylogenomic databases

OrthoDBiVOG09000071

Family and domain databases

HAMAPiMF_04052 ADV_CAPSP, 1 hit
InterProiView protein in InterPro
IPR002605 Adeno_Penton_B
PfamiView protein in Pfam
PF01686 Adeno_Penton_B, 1 hit

Sequencei

Sequence statusi: Complete.

P03276-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQRAAMYEEG PPPSYESVVS AAPVAAALGS PFDAPLDPPF VPPRYLRPTG
60 70 80 90 100
GRNSIRYSEL APLFDTTRVY LVDNKSTDVA SLNYQNDHSN FLTTVIQNND
110 120 130 140 150
YSPGEASTQT INLDDRSHWG GDLKTILHTN MPNVNEFMFT NKFKARVMVS
160 170 180 190 200
RSLTKDKQVE LKYEWVEFTL PEGNYSETMT IDLMNNAIVE HYLKVGRQNG
210 220 230 240 250
VLESDIGVKF DTRNFRLGFD PVTGLVMPGV YTNEAFHPDI ILLPGCGVDF
260 270 280 290 300
THSRLSNLLG IRKRQPFQEG FRITYDDLEG GNIPALLDVD AYQASLKDDT
310 320 330 340 350
EQGGDGAGGG NNSGSGAEEN SNAAAAAMQP VEDMNDHAIR GDTFATRAEE
360 370 380 390 400
KRAEAEAAAE AAAPAAQPEV EKPQKKPVIK PLTEDSKKRS YNLISNDSTF
410 420 430 440 450
TQYRSWYLAY NYGDPQTGIR SWTLLCTPDV TCGSEQVYWS LPDMMQDPVT
460 470 480 490 500
FRSTSQISNF PVVGAELLPV HSKSFYNDQA VYSQLIRQFT SLTHVFNRFP
510 520 530 540 550
ENQILARPPA PTITTVSENV PALTDHGTLP LRNSIGGVQR VTITDARRRT
560 570
CPYVYKALGI VSPRVLSSRT F
Length:571
Mass (Da):63,255
Last modified:July 21, 1986 - v1
Checksum:iE9807342982B1BC7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01917 Genomic DNA Translation: AAA92211.1
PIRiA03847 XZAD32
RefSeqiAP_000170.1, AC_000007.1
NP_040521.1, NC_001405.1

Genome annotation databases

GeneIDi2652996
KEGGivg:2652996

Similar proteinsi

Cross-referencesi

Web resourcesi

Virus Particle ExploreR db

Icosahedral capsid structure

Virus Particle ExploreR db

Icosahedral capsid structure in complex with an ad2 N-terminal fiber peptide

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01917 Genomic DNA Translation: AAA92211.1
PIRiA03847 XZAD32
RefSeqiAP_000170.1, AC_000007.1
NP_040521.1, NC_001405.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X9PX-ray3.30A49-571[»]
1X9TX-ray3.50A49-571[»]
2C6SX-ray3.60A/B/C/D/E/F/G/H/I/J/K/L/M/N/O49-571[»]
2C9Felectron microscopy16.50A/B/C/D/E49-571[»]
2C9Gelectron microscopy9.30A/B/C/D/E49-571[»]
4V4Uelectron microscopy10.00A/B/C/D/E49-571[»]
ProteinModelPortaliP03276
SMRiP03276
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

ELMiP03276

PTM databases

iPTMnetiP03276

Proteomic databases

PRIDEiP03276

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2652996
KEGGivg:2652996

Phylogenomic databases

OrthoDBiVOG09000071

Miscellaneous databases

EvolutionaryTraceiP03276

Family and domain databases

HAMAPiMF_04052 ADV_CAPSP, 1 hit
InterProiView protein in InterPro
IPR002605 Adeno_Penton_B
PfamiView protein in Pfam
PF01686 Adeno_Penton_B, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCAPSP_ADE02
AccessioniPrimary (citable) accession number: P03276
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 23, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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