UniProtKB - P03265 (DNB2_ADE05)
DNA-binding protein
DBP
Functioni
Plays a role in the elongation phase of viral strand displacement replication by unwinding the template in an ATP-independent fashion, employing its capacity to form multimers. Also enhances the rate of initiation. Released from template upon second strand synthesis. Assembles in complex with viral pTP, viral pol, host NFIA and host POU2F1/OCT1 on viral origin of replication. Covers the whole ssDNA genome during synthesis. The complementary strand synthesis induces its relese from DNA template. May inhibit cellular transcription mediated by the interaction between host SRCAP and CBP.
UniRule annotation2 PublicationsSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 284 | Zinc 1UniRule annotationCombined sources | 1 | |
Metal bindingi | 286 | Zinc 1UniRule annotationCombined sources | 1 | |
Metal bindingi | 339 | Zinc 1UniRule annotationCombined sources | 1 | |
Metal bindingi | 355 | Zinc 1UniRule annotationCombined sources | 1 | |
Metal bindingi | 396 | Zinc 2UniRule annotationCombined sources | 1 | |
Metal bindingi | 398 | Zinc 2UniRule annotationCombined sources | 1 | |
Metal bindingi | 450 | Zinc 2UniRule annotationCombined sources | 1 | |
Metal bindingi | 467 | Zinc 2UniRule annotationCombined sources | 1 |
GO - Molecular functioni
- DNA binding Source: UniProtKB
- identical protein binding Source: CAFA
- single-stranded DNA binding Source: CAFA
- zinc ion binding Source: CAFA
GO - Biological processi
- DNA duplex unwinding Source: UniProtKB
- DNA unwinding involved in DNA replication Source: InterPro
- positive regulation of DNA replication Source: UniProtKB
- transcription, DNA-templated Source: UniProtKB-UniRule
- viral DNA genome replication Source: UniProtKB
- viral DNA strand displacement replication Source: UniProtKB
Keywordsi
Molecular function | DNA-binding |
Biological process | DNA replication, Host-virus interaction, Viral DNA replication |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: DNA-binding proteinUniRule annotationShort name: DBPUniRule annotation Alternative name(s): Early 2A proteinUniRule annotation Early E2A DNA-binding proteinUniRule annotation |
Gene namesi | Name:DBPUniRule annotation |
Organismi | Human adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5) |
Taxonomic identifieri | 28285 [NCBI] |
Taxonomic lineagei | Viruses › Varidnaviria › Bamfordvirae › Preplasmiviricota › Tectiliviricetes › Rowavirales › Adenoviridae › Mastadenovirus › |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] |
Proteomesi |
|
Subcellular locationi
- Host nucleus UniRule annotation Note: Accumulates in infected cells.UniRule annotation
Keywords - Cellular componenti
Host nucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000221679 | 1 – 529 | DNA-binding proteinAdd BLAST | 529 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 195 | Phosphotyrosine; by hostUniRule annotation | 1 |
Keywords - PTMi
PhosphoproteinInteractioni
Subunit structurei
Homomultimerizes on viral ssDNA bound to pTP.
Forms a initiation complex with viral polymerase, pTP and hosts NFIA and POU2F1/OCT1.
Interacts with host SRCAP.
UniRule annotationGO - Molecular functioni
- identical protein binding Source: CAFA
Protein-protein interaction databases
IntActi | P03265, 5 interactors |
MINTi | P03265 |
Structurei
Secondary structure
3D structure databases
SMRi | P03265 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P03265 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 107 | DisorderedSequence analysisAdd BLAST | 107 | |
Regioni | 125 – 166 | DisorderedSequence analysisAdd BLAST | 42 | |
Regioni | 297 – 331 | Flexible loopUniRule annotationAdd BLAST | 35 | |
Regioni | 513 – 529 | C-terminal arm, DBP bindingUniRule annotationAdd BLAST | 17 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 22 | Basic and acidic residuesSequence analysisAdd BLAST | 22 | |
Compositional biasi | 47 – 62 | Basic and acidic residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 137 – 156 | Basic and acidic residuesSequence analysisAdd BLAST | 20 |
Domaini
Sequence similaritiesi
Family and domain databases
DisProti | DP00003 |
Gene3Di | 1.10.269.10, 1 hit 3.90.148.10, 2 hits |
HAMAPi | MF_04054, ADV_DNB2, 1 hit |
InterProi | View protein in InterPro IPR036367, Ad_DBP_C_sf IPR036368, ADBP_zn-bd_sf IPR003176, Adenovirus_DNA-bd_a IPR036362, Adenovirus_DNA-bd_N_sf IPR005376, Adenovirus_DNA-bd_zn-bd IPR037540, ADV_DNB2 |
Pfami | View protein in Pfam PF02236, Viral_DNA_bi, 1 hit PF03728, Viral_DNA_Zn_bi, 2 hits |
SUPFAMi | SSF47724, SSF47724, 1 hit SSF57917, SSF57917, 2 hits |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MASREEEQRE TTPERGRGAA RRPPTMEDVS SPSPSPPPPR APPKKRMRRR
60 70 80 90 100
IESEDEEDSS QDALVPRTPS PRPSTSAADL AIAPKKKKKR PSPKPERPPS
110 120 130 140 150
PEVIVDSEEE REDVALQMVG FSNPPVLIKH GKGGKRTVRR LNEDDPVARG
160 170 180 190 200
MRTQEEEEEP SEAESEITVM NPLSVPIVSA WEKGMEAARA LMDKYHVDND
210 220 230 240 250
LKANFKLLPD QVEALAAVCK TWLNEEHRGL QLTFTSKKTF VTMMGRFLQA
260 270 280 290 300
YLQSFAEVTY KHHEPTGCAL WLHRCAEIEG ELKCLHGSIM INKEHVIEMD
310 320 330 340 350
VTSENGQRAL KEQSSKAKIV KNRWGRNVVQ ISNTDARCCV HDAACPANQF
360 370 380 390 400
SGKSCGMFFS EGAKAQVAFK QIKAFMQALY PNAQTGHGHL LMPLRCECNS
410 420 430 440 450
KPGHAPFLGR QLPKLTPFAL SNAEDLDADL ISDKSVLASV HHPALIVFQC
460 470 480 490 500
CNPVYRNSRA QGGGPNCDFK ISAPDLLNAL VMVRSLWSEN FTELPRMVVP
510 520
EFKWSTKHQY RNVSLPVAHS DARQNPFDF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M73260 Genomic DNA No translation available. X02997 Genomic DNA Translation: CAA26755.1 Sequence problems. |
PIRi | A03833, W7AD25 |
RefSeqi | AP_000213.1, AC_000008.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M73260 Genomic DNA No translation available. X02997 Genomic DNA Translation: CAA26755.1 Sequence problems. |
PIRi | A03833, W7AD25 |
RefSeqi | AP_000213.1, AC_000008.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1ADU | X-ray | 3.00 | A/B | 174-529 | [»] | |
1ADV | X-ray | 3.20 | A/B | 174-529 | [»] | |
1ANV | X-ray | 2.70 | A | 174-529 | [»] | |
2WAZ | X-ray | 2.30 | X | 174-529 | [»] | |
2WB0 | X-ray | 1.95 | X | 174-529 | [»] | |
SMRi | P03265 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P03265, 5 interactors |
MINTi | P03265 |
Miscellaneous databases
EvolutionaryTracei | P03265 |
Family and domain databases
DisProti | DP00003 |
Gene3Di | 1.10.269.10, 1 hit 3.90.148.10, 2 hits |
HAMAPi | MF_04054, ADV_DNB2, 1 hit |
InterProi | View protein in InterPro IPR036367, Ad_DBP_C_sf IPR036368, ADBP_zn-bd_sf IPR003176, Adenovirus_DNA-bd_a IPR036362, Adenovirus_DNA-bd_N_sf IPR005376, Adenovirus_DNA-bd_zn-bd IPR037540, ADV_DNB2 |
Pfami | View protein in Pfam PF02236, Viral_DNA_bi, 1 hit PF03728, Viral_DNA_Zn_bi, 2 hits |
SUPFAMi | SSF47724, SSF47724, 1 hit SSF57917, SSF57917, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | DNB2_ADE05 | |
Accessioni | P03265Primary (citable) accession number: P03265 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | September 29, 2021 | |
This is version 129 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families