UniProtKB - P03200 (GP350_EBVB9)
Envelope glycoprotein GP350
BLLF1
Functioni
Initiates virion attachment to host B-lymphocyte cell, leading to virus entry. Acts by binding to host CR2 at the surface of B-lymphocytes, facilitating the binding of viral glycoprotein gp42 to HLA class II molecules. Attachment triggers virion-host membrane fusion and invasion of the host cell.
Keywordsi
Biological process | Host-virus interaction |
Enzyme and pathway databases
SIGNORi | P03200 |
Names & Taxonomyi
Protein namesi | Recommended name: Envelope glycoprotein GP350Alternative name(s): Membrane antigen Short name: MA |
Gene namesi | ORF Names:BLLF1 |
Organismi | Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4) |
Taxonomic identifieri | 10377 [NCBI] |
Taxonomic lineagei | Viruses › Duplodnaviria › Heunggongvirae › Peploviricota › Herviviricetes › Herpesvirales › Herpesviridae › Gammaherpesvirinae › Lymphocryptovirus › |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] |
Proteomesi |
|
Subcellular locationi
Other locations
- Virion membrane 1 Publication; Single-pass membrane protein 1 Publication
- Host membrane 1 Publication; Single-pass membrane protein 1 Publication
Note: Most abundant component of the viral envelope.
Other locations
- host cell membrane Source: UniProtKB-SubCell
- integral component of membrane Source: UniProtKB-KW
- virion membrane Source: UniProtKB-SubCell
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 860 | Virion surfaceSequence analysisAdd BLAST | 860 | |
Transmembranei | 861 – 881 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 882 – 907 | IntravirionSequence analysisAdd BLAST | 26 |
Keywords - Cellular componenti
Host membrane, Membrane, VirionPathology & Biotechi
Biotechnological usei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 21 | E → A: 40% loss of interaction with host CR2. 1 Publication | 1 | |
Mutagenesisi | 22 | D → A: Complete loss of interaction with host CR2. 1 Publication | 1 | |
Mutagenesisi | 151 | Y → A: Complete loss of interaction with host CR2. 1 Publication | 1 | |
Mutagenesisi | 155 | E → A: 60% loss of interaction with host CR2. 1 Publication | 1 | |
Mutagenesisi | 160 | I → A: Complete loss of interaction with host CR2. 1 Publication | 1 | |
Mutagenesisi | 162 | W → A: Complete loss of interaction with host CR2. 1 Publication | 1 | |
Mutagenesisi | 201 | E → A: 30% loss of interaction with host CR2. 1 Publication | 1 | |
Mutagenesisi | 208 | D → A: 60% loss of interaction with host CR2. 1 Publication | 1 | |
Mutagenesisi | 210 | E → A: 60% loss of interaction with host CR2. 1 Publication | 1 | |
Mutagenesisi | 215 | D → A: 30% loss of interaction with host CR2. 1 Publication | 1 | |
Mutagenesisi | 296 | D → A: Complete loss of interaction with host CR2. 1 Publication | 1 |
Chemistry databases
DrugBanki | DB00632, Docosanol |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000116183 | 1 – 907 | Envelope glycoprotein GP350Add BLAST | 907 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 47 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 87 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 114 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 166 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 169 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 195 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 229 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 277 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 318 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 328 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 345 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 356 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 378 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 386 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 411 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 435 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 443 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 457 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 497 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 519 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 533 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 547 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 568 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 589 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 610 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 624 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 627 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 645 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 656 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 683 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 701 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 735 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 746 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 755 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 780 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 815 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 858 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 |
Post-translational modificationi
Keywords - PTMi
GlycoproteinProteomic databases
PRIDEi | P03200 |
Interactioni
Subunit structurei
Interacts with host CR2.
2 PublicationsProtein-protein interaction databases
IntActi | P03200, 2 interactors |
MINTi | P03200 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 423 – 830 | DisorderedSequence analysisAdd BLAST | 408 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixFamily and domain databases
InterProi | View protein in InterPro IPR007796, Herpes_BLLF1 |
Pfami | View protein in Pfam PF05109, Herpes_BLLF1, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEAALLVCQY TIQSLIHLTG EDPGFFNVEI PEFPFYPTCN VCTADVNVTI
60 70 80 90 100
NFDVGGKKHQ LDLDFGQLTP HTKAVYQPRG AFGGSENATN LFLLELLGAG
110 120 130 140 150
ELALTMRSKK LPINVTTGEE QQVSLESVDV YFQDVFGTMW CHHAEMQNPV
160 170 180 190 200
YLIPETVPYI KWDNCNSTNI TAVVRAQGLD VTLPLSLPTS AQDSNFSVKT
210 220 230 240 250
EMLGNEIDIE CIMEDGEISQ VLPGDNKFNI TCSGYESHVP SGGILTSTSP
260 270 280 290 300
VATPIPGTGY AYSLRLTPRP VSRFLGNNSI LYVFYSGNGP KASGGDYCIQ
310 320 330 340 350
SNIVFSDEIP ASQDMPTNTT DITYVGDNAT YSVPMVTSED ANSPNVTVTA
360 370 380 390 400
FWAWPNNTET DFKCKWTLTS GTPSGCENIS GAFASNRTFD ITVSGLGTAP
410 420 430 440 450
KTLIITRTAT NATTTTHKVI FSKAPESTTT SPTLNTTGFA DPNTTTGLPS
460 470 480 490 500
STHVPTNLTA PASTGPTVST ADVTSPTPAG TTSGASPVTP SPSPWDNGTE
510 520 530 540 550
SKAPDMTSST SPVTTPTPNA TSPTPAVTTP TPNATSPTPA VTTPTPNATS
560 570 580 590 600
PTLGKTSPTS AVTTPTPNAT SPTLGKTSPT SAVTTPTPNA TSPTLGKTSP
610 620 630 640 650
TSAVTTPTPN ATGPTVGETS PQANATNHTL GGTSPTPVVT SQPKNATSAV
660 670 680 690 700
TTGQHNITSS STSSMSLRPS SNPETLSPST SDNSTSHMPL LTSAHPTGGE
710 720 730 740 750
NITQVTPASI STHHVSTSSP APRPGTTSQA SGPGNSSTST KPGEVNVTKG
760 770 780 790 800
TPPQNATSPQ APSGQKTAVP TVTSTGGKAN STTGGKHTTG HGARTSTEPT
810 820 830 840 850
TDYGGDSTTP RPRYNATTYL PPSTSSKLRP RWTFTSPPVT TAQATVPVPP
860 870 880 890 900
TSQPRFSNLS MLVLQWASLA VLTLLLLLVM ADCAFRRNLS TSHTYTTPPY
DDAETYV
The sequence of this isoform differs from the canonical sequence as follows:
502-750: Missing.
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 721 | A → E in AAA45881 (PubMed:2987520).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_002070 | 502 – 750 | Missing in isoform GP220. CuratedAdd BLAST | 249 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V01555 Genomic DNA Translation: CAA24854.1 AJ507799 Genomic DNA Translation: CAD53417.1 M10593 Genomic DNA Translation: AAA45881.1 M10593 Genomic DNA Translation: AAA45880.1 |
PIRi | A43042, QQBE21 B43042, QQBE22 |
RefSeqi | YP_401667.1, NC_007605.1 |
Genome annotation databases
GeneIDi | 3783713 |
KEGGi | vg:3783713 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V01555 Genomic DNA Translation: CAA24854.1 AJ507799 Genomic DNA Translation: CAD53417.1 M10593 Genomic DNA Translation: AAA45881.1 M10593 Genomic DNA Translation: AAA45880.1 |
PIRi | A43042, QQBE21 B43042, QQBE22 |
RefSeqi | YP_401667.1, NC_007605.1 |
3D structure databases
SMRi | P03200 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P03200, 2 interactors |
MINTi | P03200 |
Chemistry databases
DrugBanki | DB00632, Docosanol |
Proteomic databases
PRIDEi | P03200 |
Protocols and materials databases
ABCDi | P03200, 1 sequenced antibody |
DNASUi | 3783713 |
Genome annotation databases
GeneIDi | 3783713 |
KEGGi | vg:3783713 |
Enzyme and pathway databases
SIGNORi | P03200 |
Family and domain databases
InterProi | View protein in InterPro IPR007796, Herpes_BLLF1 |
Pfami | View protein in Pfam PF05109, Herpes_BLLF1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GP350_EBVB9 | |
Accessioni | P03200Primary (citable) accession number: P03200 Secondary accession number(s): P03201 Q777F0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | February 23, 2022 | |
This is version 106 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families