UniProtKB - P03170 (ICP47_HHV11)
Protein
ICP47 protein
Gene
US12
Organism
Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Status
Functioni
Plays a role in the inhibition of host immune response. Binds specifically to transporters associated with antigen processing (TAP), thereby blocking peptide-binding and translocation by TAP as well as subsequent loading of peptides onto MHC class I molecules. Empty MHC I molecules are retained in the endoplasmic reticulum and ultimately directed to proteasomal degradation. In consequence, infected cells are masked for immune recognition by cytotoxic T-lymphocytes.4 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 22 | Binding to TAP1 subunitBy similarity | 1 |
GO - Biological processi
- suppression by virus of host adaptive immune response Source: UniProtKB-KW
- suppression by virus of host antigen processing and presentation Source: UniProtKB-KW
Keywordsi
Biological process | Host-virus interaction, Inhibition of host adaptive immune response by virus, Inhibition of host TAP by virus, Viral immunoevasion |
Protein family/group databases
TCDBi | 8.A.72.1.1, the immune evasion protein, icp47 (icp47) family |
Names & Taxonomyi
Protein namesi | Recommended name: ICP47 proteinAlternative name(s): Immediate-early protein IE12 Immediate-early-5 Infected cell protein 47 US12 protein Vmw12 |
Gene namesi | Name:US12 |
Organismi | Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1) |
Taxonomic identifieri | 10299 [NCBI] |
Taxonomic lineagei | Viruses › Duplodnaviria › Heunggongvirae › Peploviricota › Herviviricetes › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Simplexvirus › |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] |
Proteomesi |
|
Subcellular locationi
- Host cytoplasm 1 Publication
- Host nucleus 1 Publication
GO - Cellular componenti
- host cell cytoplasm Source: UniProtKB-SubCell
- host cell nucleus Source: UniProtKB-SubCell
Keywords - Cellular componenti
Host cytoplasm, Host nucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 24 | D → G: Reduced ability to inhibit peptide-binding to TAP. 1 Publication | 1 | |
Mutagenesisi | 31 | K → G: Reduced ability to inhibit peptide-binding to TAP. 1 Publication | 1 | |
Mutagenesisi | 32 | R → G: Reduced ability to inhibit peptide-binding to TAP. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000115810 | 1 – 88 | ICP47 proteinAdd BLAST | 88 |
Proteomic databases
PRIDEi | P03170 |
Interactioni
Subunit structurei
Interacts with host TAP1 and TAP2; these interactions inhibit the loading of peptides onto MHC class I molecules.
1 PublicationProtein-protein interaction databases
DIPi | DIP-61932N |
IntActi | P03170, 2 interactors |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P03170 |
SMRi | P03170 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P03170 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 35 | Active domainBy similarityAdd BLAST | 34 |
Domaini
The N-terminal active domain blocks peptide binding to and peptide transport by TAP.By similarity
Sequence similaritiesi
Belongs to the herpesviridae US12 family.Curated
Family and domain databases
InterProi | View protein in InterPro IPR008026, Herpes_ICP47 |
Pfami | View protein in Pfam PF05363, Herpes_US12, 1 hit |
i Sequence
Sequence statusi: Complete.
P03170-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSWALEMADT FLDTMRVGPR TYADVRDEIN KRGREDREAA RTAVHDPERP
60 70 80
LLRSPGLLPE IAPNASLGVA HRRTGGTVTD SPRNPVTR
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 14 | T → N (PubMed:6278443).Curated | 1 | |
Sequence conflicti | 14 | T → N (PubMed:11117552).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02220 Genomic DNA Translation: AAA45795.1 L00036 Genomic DNA Translation: AAA96676.1 X00428 Genomic RNA Translation: CAA25124.1 X02138 Genomic DNA Translation: CAA26066.1 X14112 Genomic DNA Translation: CAA32277.1 V00462 Genomic DNA Translation: CAA23737.1 AF290017 Genomic DNA Translation: AAG33133.1 AF290018 Genomic DNA Translation: AAG33134.1 AF324428 Genomic DNA Translation: AAK12110.1 |
PIRi | A93454, EDBE51 |
RefSeqi | YP_009137148.1, NC_001806.2 |
Genome annotation databases
GeneIDi | 2703441 |
KEGGi | vg:2703441 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02220 Genomic DNA Translation: AAA45795.1 L00036 Genomic DNA Translation: AAA96676.1 X00428 Genomic RNA Translation: CAA25124.1 X02138 Genomic DNA Translation: CAA26066.1 X14112 Genomic DNA Translation: CAA32277.1 V00462 Genomic DNA Translation: CAA23737.1 AF290017 Genomic DNA Translation: AAG33133.1 AF290018 Genomic DNA Translation: AAG33134.1 AF324428 Genomic DNA Translation: AAK12110.1 |
PIRi | A93454, EDBE51 |
RefSeqi | YP_009137148.1, NC_001806.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1QLO | NMR | - | A | 1-34 | [»] | |
BMRBi | P03170 | |||||
SMRi | P03170 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-61932N |
IntActi | P03170, 2 interactors |
Protein family/group databases
TCDBi | 8.A.72.1.1, the immune evasion protein, icp47 (icp47) family |
Proteomic databases
PRIDEi | P03170 |
Genome annotation databases
GeneIDi | 2703441 |
KEGGi | vg:2703441 |
Miscellaneous databases
EvolutionaryTracei | P03170 |
Family and domain databases
InterProi | View protein in InterPro IPR008026, Herpes_ICP47 |
Pfami | View protein in Pfam PF05363, Herpes_US12, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ICP47_HHV11 | |
Accessioni | P03170Primary (citable) accession number: P03170 Secondary accession number(s): Q99BW2, Q9E0N0, Q9E0N1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | March 1, 2004 | |
Last modified: | December 2, 2020 | |
This is version 100 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families