E7 - Protein E7 - Human papillomavirus type 16

Functionⁱ

Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. E7 protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Interferes with host histone deacetylation mediated by HDAC1 and HDAC2, leading to transcription activation. Plays also a role in the inhibition of both antiviral and antiproliferative functions of host interferon alpha. Interaction with host TMEM173/STING impairs the ability of TMEM173/STING to sense cytosolic DNA and promote the production of type I interferon (IFN-alpha and IFN-beta).UniRule annotation3 Publications

Miscellaneous

HPV16, in comparison to HPV types 6 and 11, is more often associated with malignant genital cancers in humans.

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	58 – 94	UniRule annotationAdd BLAST		37

GO - Molecular functionⁱ

activating transcription factor binding
Source: CAFA
Inferred from direct assayⁱ
- 8245034
cadmium ion binding
Source: CAFA
Inferred from direct assayⁱ
- 8245034
DNA binding Source: UniProtKB-UniRule
DNA-binding transcription factor activity Source: UniProtKB-UniRule
protein domain specific binding Source: UniProtKB-UniRule
repressing transcription factor binding
Source: CAFA
Inferred from physical interactionⁱ
- 8245034
zinc ion binding
Source: CAFA
Inferred from direct assayⁱ
- 8245034

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

modulation by virus of host G1/S transition checkpoint Source: UniProtKB-UniRule
modulation by virus of host transcription
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 10523841
positive regulation by symbiont of host transcription
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 10523841
positive regulation of actin filament polymerization
Source: UniProtKB
Inferred from direct assayⁱ
- 23729654
suppression by virus of host cysteine-type endopeptidase activity involved in apoptotic process
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 23729654
suppression by virus of host IRF9 activity
Source: UniProtKB
Inferred from direct assayⁱ
- 10388655
suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-UniRule
transcription, DNA-templated Source: UniProtKB-UniRule

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Activator, DNA-binding
Biological process	G1/S host cell cycle checkpoint dysregulation by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Modulation of host cell cycle by virus, Transcription, Transcription regulation, Viral immunoevasion
Ligand	Metal-binding, Zinc

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Protein E7UniRule annotation
Gene namesⁱ	Name:E7UniRule annotation
Organismⁱ	Human papillomavirus type 16
Taxonomic identifierⁱ	333760 [NCBI]
Taxonomic lineageⁱ	Viruses › dsDNA viruses, no RNA stage › Papillomaviridae › Firstpapillomavirinae › Alphapapillomavirus › Alphapapillomavirus 9
Virus hostⁱ	Homo sapiens (Human) [TaxID: 9606]
Proteomesⁱ	UP000115181 Componentⁱ: Genome UP000106302 Componentⁱ: Genome UP000009251 Componentⁱ: Genome

Subcellular locationⁱ

Note:

Predominantly found in the host nucleus.UniRule annotation1 Publication

GO - Cellular componentⁱ

host cell cytoplasm Source: UniProtKB-SubCell
host cell nucleus Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Host cytoplasm, Host nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	67	L → R: Complete loss of interaction with HDAC1 and transformation ability. 1 Publication	1
Mutagenesisⁱ	82 – 83	LL → RR: Complete loss of interaction with HDAC1 and transformation ability. 1 Publication	2
Mutagenesisⁱ	91	C → G: Complete loss of interaction with HDAC1 and transformation ability. 1 Publication	1

Keywords - Diseaseⁱ

Oncogene

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000133414	1 – 98	Protein E7Add BLAST		98

Post-translational modificationⁱ

Highly phosphorylated.UniRule annotation1 Publication

PTM databases

iPTMnetⁱ	P03129

iPTMnetⁱ

P03129

Expressionⁱ

Keywords - Developmental stageⁱ

Early protein

Interactionⁱ

Subunit structureⁱ

Homodimer (PubMed:11123931). Homooligomer (PubMed:15035602). Interacts with host RB1; this interaction induces dissociation of RB1-E2F1 complex thereby disrupting RB1 activity (PubMed:1316611). Interacts with host EP300; this interaction represses EP300 transcriptional activity (PubMed:12970734). Forms a complex with CHD4 and HDAC1, thereby altering the action of host histone deacetylation. A similar complex involving E7, CHD4 and HDAC2 may also form (PubMed:10228159). Interacts with protein E2; this interaction inhibits E7 oncogenic activity (PubMed:16439535).UniRule annotation6 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
CCNA2	P20248	2	EBI-866453,EBI-457097	From Homo sapiens.
CDK2	P24941	2	EBI-866453,EBI-375096	From Homo sapiens.
CHUK	O15111	2	EBI-866453,EBI-81249	From Homo sapiens.
E2F1	Q01094	2	EBI-866453,EBI-448924	From Homo sapiens.
E2F4	Q16254	2	EBI-866453,EBI-448943	From Homo sapiens.
FOXM1	Q08050-1	3	EBI-866453,EBI-866499	From a different organism.
IRF1	P10914	3	EBI-866453,EBI-1055781	From Homo sapiens.
KAT2B	Q92831	3	EBI-866453,EBI-477430	From Homo sapiens.
MYC	P01106	2	EBI-866453,EBI-447544	From Homo sapiens.
PPP2CA	P67775	3	EBI-866453,EBI-712311	From Homo sapiens.
PPP2R1A	P30153	3	EBI-866453,EBI-302388	From Homo sapiens.
RB1	P06400	22	EBI-866453,EBI-491274	From Homo sapiens.
RBL1	P28749	3	EBI-866453,EBI-971402	From Homo sapiens.
SIVA1	O15304	4	EBI-866453,EBI-520756	From Homo sapiens.
TFDP2	Q14188	2	EBI-866453,EBI-752268	From Homo sapiens.
UBR4	Q5T4S7	5	EBI-866453,EBI-1995940	From Homo sapiens.
USP11	P51784	6	EBI-866453,EBI-306876	From Homo sapiens.

GO - Molecular functionⁱ

activating transcription factor binding
Source: CAFA
Inferred from direct assayⁱ
- 8245034
protein domain specific binding Source: UniProtKB-UniRule
repressing transcription factor binding
Source: CAFA
Inferred from physical interactionⁱ
- 8245034

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	4263558, 7 interactors
Database of interacting proteins More...DIPⁱ	DIP-1090N
IntActⁱ	P03129, 81 interactors
Molecular INTeraction database More...MINTⁱ	P03129

Structureⁱ

3D structure databases

Database of protein disorder More...DisProtⁱ	DP00024
ProteinModelPortalⁱ	P03129
SMRⁱ	P03129
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	1 – 40	DisorderedUniRule annotation1 PublicationAdd BLAST		40

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	22 – 26	LXCXE motif; interaction with host RB1 and TMEM173/STINGUniRule annotation		5
Motifⁱ	76 – 84	Nuclear export signalUniRule annotation1 Publication		9

Sequence similaritiesⁱ

Belongs to the papillomaviridae E7 protein family.UniRule annotation

Zinc finger

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	58 – 94	UniRule annotationAdd BLAST		37

Keywords - Domainⁱ

Zinc-finger

Phylogenomic databases

KEGG Orthology (KO) More...KOⁱ	K21809
Database of Orthologous Groups More...OrthoDBⁱ	VOG090001L5

Family and domain databases

HAMAPⁱ	MF_04004 PPV_E7, 1 hit
InterProⁱ	View protein in InterPro IPR000148 Papilloma_E7
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00527 E7, 1 hit
PIRSFⁱ	PIRSF003407 Papvi_E7, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P03129-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MHGDTPTLHE YMLDLQPETT DLYCYEQLND SSEEEDEIDG PAGQAEPDRA 
        60         70         80         90 
HYNIVTFCCK CDSTLRLCVQ STHVDIRTLE DLLMGTLGIV CPICSQKP

Length:98

Mass (Da):11,022

Last modified:July 21, 1986 - v1

Checksum:ⁱ9BD612534CD2C9EB

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	K02718 Genomic DNA Translation: AAA46940.1 D00735 Genomic DNA Translation: BAA00633.1 AF003020 Genomic DNA Translation: AAB70737.1 AF003023 Genomic DNA Translation: AAB70740.1 AF003024 Genomic DNA Translation: AAB70741.1 AF003025 Genomic DNA Translation: AAB70742.1 AF003026 Genomic DNA Translation: AAB70743.1 U76411 Genomic DNA Translation: AAB18962.1 U76412 Genomic DNA Translation: AAB18963.1 U76413 Genomic DNA Translation: AAB18964.1 AF536179 Genomic DNA Translation: AAQ10713.1 AF536180 Genomic DNA Translation: AAQ10721.1
PIRⁱ	A03688 W7WLHS
NCBI Reference Sequences More...RefSeqⁱ	NP_041326.1, NC_001526.4

Genome annotation databases

GeneIDⁱ	1489079
KEGGⁱ	vg:1489079

Protein	Similar proteins		Species	Score	Length	Source
P03129	Protein E7		Alphapapillomavirus 9		141	UniRef100_P03129
Protein E7 (Fragment)		HPV16		92
Protein E7		Human papillomavirus		98
Protein E7 (Fragment)		HPV16		92
Protein E7 (Fragment)		HPV16		92
+183

Protein	Similar proteins		Species	Score	Length	Source
P03129	Protein E7		Alphapapillomavirus 9		141	UniRef90_P03129
Protein E7 (Fragment)		HPV16		92
Protein E7		Human papillomavirus		98
Protein E7 (Fragment)		HPV16		92
Protein E7 (Fragment)		HPV16		92
+217

Protein	Similar proteins		Species	Score	Length	Source
P03129	Protein E7		Alphapapillomavirus 9		141	UniRef50_P03129
Protein E7 (Fragment)		HPV16		92
Protein E7		Human papillomavirus		98
Protein E7 (Fragment)		HPV16		92
Protein E7 (Fragment)		HPV16		92
+217

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	K02718 Genomic DNA Translation: AAA46940.1 D00735 Genomic DNA Translation: BAA00633.1 AF003020 Genomic DNA Translation: AAB70737.1 AF003023 Genomic DNA Translation: AAB70740.1 AF003024 Genomic DNA Translation: AAB70741.1 AF003025 Genomic DNA Translation: AAB70742.1 AF003026 Genomic DNA Translation: AAB70743.1 U76411 Genomic DNA Translation: AAB18962.1 U76412 Genomic DNA Translation: AAB18963.1 U76413 Genomic DNA Translation: AAB18964.1 AF536179 Genomic DNA Translation: AAQ10713.1 AF536180 Genomic DNA Translation: AAQ10721.1
PIRⁱ	A03688 W7WLHS
RefSeqⁱ	NP_041326.1, NC_001526.4

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	4YOZ	X-ray	2.25	B	21-29	[»]
DisProtⁱ	DP00024
ProteinModelPortalⁱ	P03129
SMRⁱ	P03129
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4263558, 7 interactors
DIPⁱ	DIP-1090N
IntActⁱ	P03129, 81 interactors
MINTⁱ	P03129

PTM databases

iPTMnetⁱ	P03129

iPTMnetⁱ

P03129

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

GeneIDⁱ	1489079
KEGGⁱ	vg:1489079

Phylogenomic databases

KOⁱ	K21809
OrthoDBⁱ	VOG090001L5

Family and domain databases

HAMAPⁱ	MF_04004 PPV_E7, 1 hit
InterProⁱ	View protein in InterPro IPR000148 Papilloma_E7
Pfamⁱ	View protein in Pfam PF00527 E7, 1 hit
PIRSFⁱ	PIRSF003407 Papvi_E7, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	VE7_HPV16
Accessionⁱ	P03129Primary (citable) accession number: P03129
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
	Last sequence update:	July 21, 1986
	Last modified:	November 7, 2018
	This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Viral Protein Annotation Program

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P03129 (VE7_HPV16)

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Protein E7

E7

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Keywords - Diseasei

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Keywords - Developmental stagei

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Zinc finger

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

Keywords - Diseaseⁱ

PTM / Processingⁱ

Expressionⁱ

Keywords - Developmental stageⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ