UniProtKB - P03129 (VE7_HPV16)
Protein E7
E7
Functioni
Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. E7 protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Interferes with host histone deacetylation mediated by HDAC1 and HDAC2, leading to transcription activation. Plays also a role in the inhibition of both antiviral and antiproliferative functions of host interferon alpha. Interaction with host TMEM173/STING impairs the ability of TMEM173/STING to sense cytosolic DNA and promote the production of type I interferon (IFN-alpha and IFN-beta).
UniRule annotation3 PublicationsMiscellaneous
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 58 – 94 | UniRule annotationAdd BLAST | 37 |
GO - Molecular functioni
- cadmium ion binding Source: CAFA
- DNA binding Source: UniProtKB-UniRule
- DNA-binding transcription factor activity Source: UniProtKB-UniRule
- DNA-binding transcription factor binding Source: CAFA
- protein domain specific binding Source: UniProtKB-UniRule
- zinc ion binding Source: CAFA
GO - Biological processi
- modulation by virus of host G1/S transition checkpoint Source: UniProtKB-UniRule
- modulation by virus of host transcription Source: UniProtKB
- positive regulation by symbiont of host transcription Source: UniProtKB
- positive regulation of actin filament polymerization Source: UniProtKB
- suppression by virus of host cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
- suppression by virus of host JAK-STAT cascade via inhibition of host IRF9 activity Source: UniProtKB
- suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-UniRule
- transcription, DNA-templated Source: UniProtKB-UniRule
Keywordsi
Names & Taxonomyi
Protein namesi | Recommended name: Protein E7UniRule annotation |
Gene namesi | Name:E7UniRule annotation |
Organismi | Human papillomavirus type 16 |
Taxonomic identifieri | 333760 [NCBI] |
Taxonomic lineagei | Viruses › Monodnaviria › Shotokuvirae › Cossaviricota › Papovaviricetes › Zurhausenvirales › Papillomaviridae › Firstpapillomavirinae › Alphapapillomavirus › |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] |
Proteomesi |
|
Subcellular locationi
- Host cytoplasm UniRule annotation2 Publications
- Host nucleus UniRule annotation1 Publication Note: Predominantly found in the host nucleus.UniRule annotation1 Publication
Keywords - Cellular componenti
Host cytoplasm, Host nucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 67 | L → R: Complete loss of interaction with HDAC1 and transformation ability. 1 Publication | 1 | |
Mutagenesisi | 82 – 83 | LL → RR: Complete loss of interaction with HDAC1 and transformation ability. 1 Publication | 2 | |
Mutagenesisi | 91 | C → G: Complete loss of interaction with HDAC1 and transformation ability. 1 Publication | 1 |
Keywords - Diseasei
OncogeneChemistry databases
ChEMBLi | CHEMBL4630866 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000133414 | 1 – 98 | Protein E7Add BLAST | 98 |
Post-translational modificationi
Proteomic databases
PRIDEi | P03129 |
PTM databases
iPTMneti | P03129 |
Interactioni
Subunit structurei
Homodimer (PubMed:11123931). Homooligomer (PubMed:15035602).
Interacts with host RB1; this interaction induces dissociation of RB1-E2F1 complex thereby disrupting RB1 activity (PubMed:1316611).
Interacts with host EP300; this interaction represses EP300 transcriptional activity (PubMed:12970734).
Forms a complex with CHD4 and HDAC1, thereby altering the action of host histone deacetylation. A similar complex involving E7, CHD4 and HDAC2 may also form (PubMed:10228159).
Interacts with protein E2; this interaction inhibits E7 oncogenic activity (PubMed:16439535).
UniRule annotation6 PublicationsBinary interactionsi
P03129
GO - Molecular functioni
- DNA-binding transcription factor binding Source: CAFA
- protein domain specific binding Source: UniProtKB-UniRule
Protein-protein interaction databases
BioGRIDi | 4263558, 183 interactors |
DIPi | DIP-1090N |
IntActi | P03129, 120 interactors |
MINTi | P03129 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 40 | E7 terminal domainUniRule annotation1 PublicationAdd BLAST | 40 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 22 – 26 | LXCXE motif; interaction with host RB1 and TMEM173/STINGUniRule annotation | 5 | |
Motifi | 76 – 84 | Nuclear export signalUniRule annotation1 Publication | 9 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 58 – 94 | UniRule annotationAdd BLAST | 37 |
Keywords - Domaini
Zinc-fingerFamily and domain databases
DisProti | DP00024 |
HAMAPi | MF_04004, PPV_E7, 1 hit |
InterProi | View protein in InterPro IPR000148, Papilloma_E7 |
Pfami | View protein in Pfam PF00527, E7, 1 hit |
PIRSFi | PIRSF003407, Papvi_E7, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MHGDTPTLHE YMLDLQPETT DLYCYEQLND SSEEEDEIDG PAGQAEPDRA
60 70 80 90
HYNIVTFCCK CDSTLRLCVQ STHVDIRTLE DLLMGTLGIV CPICSQKP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K02718 Genomic DNA Translation: AAA46940.1 D00735 Genomic DNA Translation: BAA00633.1 AF003020 Genomic DNA Translation: AAB70737.1 AF003023 Genomic DNA Translation: AAB70740.1 AF003024 Genomic DNA Translation: AAB70741.1 AF003025 Genomic DNA Translation: AAB70742.1 AF003026 Genomic DNA Translation: AAB70743.1 U76411 Genomic DNA Translation: AAB18962.1 U76412 Genomic DNA Translation: AAB18963.1 U76413 Genomic DNA Translation: AAB18964.1 AF536179 Genomic DNA Translation: AAQ10713.1 AF536180 Genomic DNA Translation: AAQ10721.1 |
PIRi | A03688, W7WLHS |
RefSeqi | NP_041326.1, NC_001526.4 |
Genome annotation databases
GeneIDi | 1489079 |
KEGGi | vg:1489079 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K02718 Genomic DNA Translation: AAA46940.1 D00735 Genomic DNA Translation: BAA00633.1 AF003020 Genomic DNA Translation: AAB70737.1 AF003023 Genomic DNA Translation: AAB70740.1 AF003024 Genomic DNA Translation: AAB70741.1 AF003025 Genomic DNA Translation: AAB70742.1 AF003026 Genomic DNA Translation: AAB70743.1 U76411 Genomic DNA Translation: AAB18962.1 U76412 Genomic DNA Translation: AAB18963.1 U76413 Genomic DNA Translation: AAB18964.1 AF536179 Genomic DNA Translation: AAQ10713.1 AF536180 Genomic DNA Translation: AAQ10721.1 |
PIRi | A03688, W7WLHS |
RefSeqi | NP_041326.1, NC_001526.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4YOZ | X-ray | 2.25 | B | 21-29 | [»] | |
6APN | X-ray | 2.22 | A/B | 82-90 | [»] | |
BMRBi | P03129 | |||||
SMRi | P03129 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263558, 183 interactors |
DIPi | DIP-1090N |
IntActi | P03129, 120 interactors |
MINTi | P03129 |
Chemistry databases
ChEMBLi | CHEMBL4630866 |
PTM databases
iPTMneti | P03129 |
Proteomic databases
PRIDEi | P03129 |
Protocols and materials databases
ABCDi | P03129, 4 sequenced antibodies |
DNASUi | 1489079 |
Genome annotation databases
GeneIDi | 1489079 |
KEGGi | vg:1489079 |
Family and domain databases
DisProti | DP00024 |
HAMAPi | MF_04004, PPV_E7, 1 hit |
InterProi | View protein in InterPro IPR000148, Papilloma_E7 |
Pfami | View protein in Pfam PF00527, E7, 1 hit |
PIRSFi | PIRSF003407, Papvi_E7, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | VE7_HPV16 | |
Accessioni | P03129Primary (citable) accession number: P03129 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | February 23, 2022 | |
This is version 144 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families