UniProtKB - P03121 (VE2_CRPVK)
Regulatory protein E2
E2
Functioni
Plays a role in the initiation of viral DNA replication. A dimer of E2 interacts with a dimer of E1 in order to improve specificity of E1 DNA binding activity. Once the complex recognizes and binds DNA at specific sites, the E2 dimer is removed from DNA. E2 also regulates viral transcription through binding to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory regions of the viral genome. Activates or represses transcription depending on E2RE's position with regards to proximal promoter elements including the TATA-box. Repression occurs by sterically hindering the assembly of the transcription initiation complex.
UniRule annotationGO - Molecular functioni
- DNA binding Source: UniProtKB-UniRule
- DNA-binding transcription factor activity Source: UniProtKB-UniRule
- nucleotide binding Source: UniProtKB-UniRule
GO - Biological processi
- DNA replication Source: UniProtKB-KW
- regulation of DNA replication Source: UniProtKB-UniRule
- transcription, DNA-templated Source: UniProtKB-UniRule
- viral DNA genome replication Source: UniProtKB-UniRule
Keywordsi
Molecular function | Activator, DNA-binding, Repressor |
Biological process | DNA replication, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Regulatory protein E2UniRule annotation |
Gene namesi | Name:E2UniRule annotation |
Organismi | Cottontail rabbit papillomavirus (strain Kansas) (CRPV) (Papillomavirus sylvilagi) |
Taxonomic identifieri | 31553 [NCBI] |
Taxonomic lineagei | Viruses › Monodnaviria › Shotokuvirae › Cossaviricota › Papovaviricetes › Zurhausenvirales › Papillomaviridae › Firstpapillomavirinae › Kappapapillomavirus › |
Virus hosti | Sylvilagus floridanus (Cottontail rabbit) [TaxID: 9988] |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000133245 | 1 – 390 | Regulatory protein E2Add BLAST | 390 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 313 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)UniRule annotation |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationInteractioni
Subunit structurei
Binds DNA as homodimer.
Interacts with protein E1; this interaction greatly increases E1 DNA-binding activity.
Interacts with protein L1; this interaction enhances E2-dependent replication and transcription activation.
Interacts with protein L2; this interaction inhibits E2 transcriptional activity but not DNA replication function E2.
Interacts with protein E7; this interaction inhibits E7 oncogenic activity.
Interacts with host TAF1; this interaction modulates E2-dependent transcriptional regulation.
Interacts with host BRD4; this interaction mediates E2 transcriptional activation function. Additionally, the interaction with host BRD4 on mitotic chromosomes mediates tethering of the viral genome.
Interacts with host TOPBP1; this interaction is required for optimal viral DNA replication.
UniRule annotationProtein-protein interaction databases
IntActi | P03121, 1 interactor |
MINTi | P03121 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 202 | Transactivation domainUniRule annotationAdd BLAST | 202 | |
Regioni | 199 – 296 | DisorderedSequence analysisAdd BLAST | 98 | |
Regioni | 306 – 390 | DNA-binding domainUniRule annotationAdd BLAST | 85 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 234 – 251 | Basic residuesSequence analysisAdd BLAST | 18 |
Sequence similaritiesi
Family and domain databases
Gene3Di | 1.10.287.30, 1 hit 2.170.200.10, 1 hit 3.30.70.330, 1 hit |
HAMAPi | MF_04001, PPV_E2, 1 hit |
InterProi | View protein in InterPro IPR035975, E2/EBNA1_C_sf IPR012677, Nucleotide-bd_a/b_plait_sf IPR000427, Papillomavirus_E2_C IPR001866, Papillomavirus_E2_N IPR033668, Reg_prot_E2 IPR036050, Regulatory_protein_E2_N IPR042503, Regulatory_protein_E2_N_1 IPR042504, Regulatory_protein_E2_N_2 |
Pfami | View protein in Pfam PF00511, PPV_E2_C, 1 hit PF00508, PPV_E2_N, 1 hit |
SUPFAMi | SSF51332, SSF51332, 1 hit SSF54957, SSF54957, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MEALSQRLDS IQEELLSLYE KESTSLESQL QHWNLLRKEQ VLLHFCKKHG
60 70 80 90 100
IRQLGYTPVP SLLTSQECAK QAIEMVLYIE SLLRSPYSDE PWTLQDTSRE
110 120 130 140 150
RFESPPQKTF KKNPAIVEVY YDGDRGNNNE YTLWGIFIIG NADGEWVKTE
160 170 180 190 200
SGVDYRGIYY VDSEGNYVYY VDFSTDAGRF AANGHYDVVF QNMRLSSSVT
210 220 230 240 250
SSPQPLVSAP EDTVPEEAPD SAVPAAQKKT GPKTTRTLGR RRSRSPGVQR
260 270 280 290 300
RPAKQRKQAA PDEADSAAGD IRPPAPEDVG RRTTTVGRTP PGRNRRLREL
310 320 330 340 350
ITEASDPPVI CLKGGHNQLK CLRYRLKSKH SSLFDCISTT WSWVDTTSTC
360 370 380 390
RLGSGRMLIK FADSEQRDKF LSRVPLPSTT QVFLGNFYGL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K02708 Genomic DNA No translation available. |
PIRi | A03671, W2WLRB |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K02708 Genomic DNA No translation available. |
PIRi | A03671, W2WLRB |
3D structure databases
SMRi | P03121 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P03121, 1 interactor |
MINTi | P03121 |
Family and domain databases
Gene3Di | 1.10.287.30, 1 hit 2.170.200.10, 1 hit 3.30.70.330, 1 hit |
HAMAPi | MF_04001, PPV_E2, 1 hit |
InterProi | View protein in InterPro IPR035975, E2/EBNA1_C_sf IPR012677, Nucleotide-bd_a/b_plait_sf IPR000427, Papillomavirus_E2_C IPR001866, Papillomavirus_E2_N IPR033668, Reg_prot_E2 IPR036050, Regulatory_protein_E2_N IPR042503, Regulatory_protein_E2_N_1 IPR042504, Regulatory_protein_E2_N_2 |
Pfami | View protein in Pfam PF00511, PPV_E2_C, 1 hit PF00508, PPV_E2_N, 1 hit |
SUPFAMi | SSF51332, SSF51332, 1 hit SSF54957, SSF54957, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | VE2_CRPVK | |
Accessioni | P03121Primary (citable) accession number: P03121 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | June 2, 2021 | |
This is version 112 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families