UniProtKB - P03120 (VE2_HPV16)
Regulatory protein E2
E2
Functioni
Plays a role in the initiation of viral DNA replication. A dimer of E2 interacts with a dimer of E1 in order to improve specificity of E1 DNA binding activity. Once the complex recognizes and binds DNA at specific sites, the E2 dimer is removed from DNA. E2 also regulates viral transcription through binding to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory regions of the viral genome. Activates or represses transcription depending on E2RE's position with regards to proximal promoter elements including the TATA-box. Repression occurs by sterically hindering the assembly of the transcription initiation complex.
UniRule annotation6 PublicationsMiscellaneous
GO - Molecular functioni
- DNA binding Source: UniProtKB-UniRule
- DNA-binding transcription factor activity Source: UniProtKB-UniRule
- nucleotide binding Source: UniProtKB-UniRule
GO - Biological processi
- DNA replication Source: UniProtKB-KW
- regulation of DNA replication Source: UniProtKB-UniRule
- transcription, DNA-templated Source: UniProtKB-UniRule
- viral DNA genome replication Source: UniProtKB-UniRule
Keywordsi
Molecular function | Activator, DNA-binding, Repressor |
Biological process | DNA replication, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Regulatory protein E2UniRule annotation |
Gene namesi | Name:E2UniRule annotation |
Organismi | Human papillomavirus type 16 |
Taxonomic identifieri | 333760 [NCBI] |
Taxonomic lineagei | Viruses › Monodnaviria › Shotokuvirae › Cossaviricota › Papovaviricetes › Zurhausenvirales › Papillomaviridae › Firstpapillomavirinae › Alphapapillomavirus › |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] |
Proteomesi |
|
Subcellular locationi
- Host nucleus UniRule annotation4 Publications
Keywords - Cellular componenti
Host nucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 292 | K → R: Complete loss of sumoylation. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL4656 |
DrugCentrali | P03120 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000133195 | 1 – 365 | Regulatory protein E2Add BLAST | 365 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 243 | Phosphoserine; by host1 Publication | 1 | |
Cross-linki | 292 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)UniRule annotation1 Publication |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
PRIDEi | P03120 |
PTM databases
iPTMneti | P03120 |
Interactioni
Subunit structurei
Binds DNA as homodimer.
Interacts with protein E1; this interaction greatly increases E1 DNA-binding activity.
Interacts with protein L1; this interaction enhances E2-dependent replication and transcription activation.
Interacts with protein L2; this interaction inhibits E2 transcriptional activity but not DNA replication function E2.
Interacts with protein E7; this interaction inhibits E7 oncogenic activity.
Interacts with host TAF1; this interaction modulates E2-dependent transcriptional regulation.
Interacts with host BRD4; this interaction mediates E2 transcriptional activation function. Additionally, the interaction with host BRD4 on mitotic chromosomes mediates tethering of the viral genome.
Interacts with host TOPBP1; this interaction is required for optimal viral DNA replication.
UniRule annotation9 PublicationsBinary interactionsi
P03120
Protein-protein interaction databases
BioGRIDi | 4263559, 2 interactors |
DIPi | DIP-44574N |
IntActi | P03120, 64 interactors |
MINTi | P03120 |
Chemistry databases
BindingDBi | P03120 |
Structurei
Secondary structure
3D structure databases
BMRBi | P03120 |
SASBDBi | P03120 |
SMRi | P03120 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P03120 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 200 | Transactivation domainUniRule annotation1 PublicationAdd BLAST | 200 | |
Regioni | 285 – 365 | DNA-binding domainUniRule annotation1 PublicationAdd BLAST | 81 |
Sequence similaritiesi
Family and domain databases
DisProti | DP01428 |
Gene3Di | 1.10.287.30, 1 hit 2.170.200.10, 1 hit 3.30.70.330, 1 hit |
HAMAPi | MF_04001, PPV_E2, 1 hit |
InterProi | View protein in InterPro IPR035975, E2/EBNA1_C_sf IPR012677, Nucleotide-bd_a/b_plait_sf IPR000427, Papillomavirus_E2_C IPR001866, Papillomavirus_E2_N IPR033668, Reg_prot_E2 IPR036050, Regulatory_protein_E2_N IPR042503, Regulatory_protein_E2_N_1 IPR042504, Regulatory_protein_E2_N_2 |
Pfami | View protein in Pfam PF00511, PPV_E2_C, 1 hit PF00508, PPV_E2_N, 1 hit |
SUPFAMi | SSF51332, SSF51332, 1 hit SSF54957, SSF54957, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
METLCQRLNV CQDKILTHYE NDSTDLRDHI DYWKHMRLEC AIYYKAREMG
60 70 80 90 100
FKHINHQVVP TLAVSKNKAL QAIELQLTLE TIYNSQYSNE KWTLQDVSLE
110 120 130 140 150
VYLTAPTGCI KKHGYTVEVQ FDGDICNTMH YTNWTHIYIC EEASVTVVEG
160 170 180 190 200
QVDYYGLYYV HEGIRTYFVQ FKDDAEKYSK NKVWEVHAGG QVILCPTSVF
210 220 230 240 250
SSNEVSSPEI IRQHLANHPA ATHTKAVALG TEETQTTIQR PRSEPDTGNP
260 270 280 290 300
CHTTKLLHRD SVDSAPILTA FNSSHKGRIN CNSNTTPIVH LKGDANTLKC
310 320 330 340 350
LRYRFKKHCT LYTAVSSTWH WTGHNVKHKS AIVTLTYDSE WQRDQFLSQV
360
KIPKTITVST GFMSI
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 77 | L → P in AAQ10715 (Ref. 2) Curated | 1 | |
Sequence conflicti | 210 | I → T in AAQ10715 (Ref. 2) Curated | 1 | |
Sequence conflicti | 219 | P → S in AAQ10715 (Ref. 2) Curated | 1 | |
Sequence conflicti | 310 | T → K in AAQ10715 (Ref. 2) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K02718 Genomic DNA Translation: AAA46941.1 AF536179 Genomic DNA Translation: AAQ10715.1 |
PIRi | A03669, W2WLHS |
RefSeqi | NP_041328.1, NC_001526.4 |
Genome annotation databases
GeneIDi | 1489080 |
KEGGi | vg:1489080 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K02718 Genomic DNA Translation: AAA46941.1 AF536179 Genomic DNA Translation: AAQ10715.1 |
PIRi | A03669, W2WLHS |
RefSeqi | NP_041328.1, NC_001526.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1BY9 | X-ray | 2.20 | A | 285-365 | [»] | |
1DTO | X-ray | 1.90 | A | 1-201 | [»] | |
1R8P | NMR | - | A/B | 286-365 | [»] | |
1ZZF | NMR | - | A/B | 286-365 | [»] | |
2NNU | X-ray | 1.59 | A | 1-201 | [»] | |
2Q79 | X-ray | 1.80 | A | 285-365 | [»] | |
3MI7 | X-ray | 2.20 | X | 283-365 | [»] | |
BMRBi | P03120 | |||||
SASBDBi | P03120 | |||||
SMRi | P03120 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263559, 2 interactors |
DIPi | DIP-44574N |
IntActi | P03120, 64 interactors |
MINTi | P03120 |
Chemistry databases
BindingDBi | P03120 |
ChEMBLi | CHEMBL4656 |
DrugCentrali | P03120 |
PTM databases
iPTMneti | P03120 |
Proteomic databases
PRIDEi | P03120 |
Protocols and materials databases
DNASUi | 1489080 |
Genome annotation databases
GeneIDi | 1489080 |
KEGGi | vg:1489080 |
Miscellaneous databases
EvolutionaryTracei | P03120 |
PROi | PR:P03120 |
Family and domain databases
DisProti | DP01428 |
Gene3Di | 1.10.287.30, 1 hit 2.170.200.10, 1 hit 3.30.70.330, 1 hit |
HAMAPi | MF_04001, PPV_E2, 1 hit |
InterProi | View protein in InterPro IPR035975, E2/EBNA1_C_sf IPR012677, Nucleotide-bd_a/b_plait_sf IPR000427, Papillomavirus_E2_C IPR001866, Papillomavirus_E2_N IPR033668, Reg_prot_E2 IPR036050, Regulatory_protein_E2_N IPR042503, Regulatory_protein_E2_N_1 IPR042504, Regulatory_protein_E2_N_2 |
Pfami | View protein in Pfam PF00511, PPV_E2_C, 1 hit PF00508, PPV_E2_N, 1 hit |
SUPFAMi | SSF51332, SSF51332, 1 hit SSF54957, SSF54957, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | VE2_HPV16 | |
Accessioni | P03120Primary (citable) accession number: P03120 Secondary accession number(s): Q71BI4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | February 23, 2022 | |
This is version 168 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families