UniProtKB - P03116 (VE1_BPV1)
Replication protein E1
E1
Functioni
ATP-dependent DNA helicase required for initiation of viral DNA replication. It forms a complex with the viral E2 protein. The E1-E2 complex binds to the replication origin which contains binding sites for both proteins. During the initial step, a dimer of E1 interacts with a dimer of protein E2 leading to a complex that binds the viral origin of replication with high specificity. Then, a second dimer of E1 displaces the E2 dimer in an ATP-dependent manner to form the E1 tetramer. Following this, two E1 monomers are added to each half of the site, which results in the formation of two E1 trimers on the viral ori. Subsequently, two hexamers will be created. The double hexamer acts as a bi-directional helicase machinery and unwinds the viral DNA and then recruits the host DNA polymerase to start replication.
UniRule annotation4 PublicationsCatalytic activityi
- EC:3.6.4.12UniRule annotation
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 433 – 440 | ATPUniRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- ATP hydrolysis activity Source: RHEA
- DNA binding Source: UniProtKB-UniRule
- DNA helicase activity Source: UniProtKB-UniRule
GO - Biological processi
- DNA replication Source: UniProtKB-UniRule
Keywordsi
Molecular function | DNA-binding, Helicase, Hydrolase |
Biological process | DNA replication |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Replication protein E1UniRule annotation (EC:3.6.4.12UniRule annotation)Alternative name(s): ATP-dependent helicase E1UniRule annotation |
Gene namesi | Name:E1UniRule annotation |
Organismi | Bovine papillomavirus type 1 |
Taxonomic identifieri | 337052 [NCBI] |
Taxonomic lineagei | Viruses › Monodnaviria › Shotokuvirae › Cossaviricota › Papovaviricetes › Zurhausenvirales › Papillomaviridae › Firstpapillomavirinae › Deltapapillomavirus |
Virus hosti | Bos taurus (Bovine) [TaxID: 9913] |
Proteomesi |
|
Subcellular locationi
- Host nucleus UniRule annotation1 Publication
Keywords - Cellular componenti
Host nucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 84 – 85 | KR → GG: About 95% loss of nuclear localization. 1 Publication | 2 | |
Mutagenesisi | 109 | S → A: About 50% increase in replication of viral DNA. 1 Publication | 1 | |
Mutagenesisi | 514 | K → R: Complete loss of sumoylation. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000133090 | 1 – 605 | Replication protein E1Add BLAST | 605 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 90 | Phosphoserine; by hostUniRule annotation | 1 | |
Modified residuei | 94 | Phosphoserine; by hostUniRule annotation | 1 | |
Modified residuei | 102 | Phosphothreonine; by host CDK11 Publication | 1 | |
Modified residuei | 109 | Phosphoserine; by host1 Publication | 1 | |
Cross-linki | 514 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)UniRule annotation1 Publication |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationPTM databases
iPTMneti | P03116 |
Interactioni
Subunit structurei
Can form hexamers.
Interacts with E2 protein; this interaction increases E1 DNA binding specificity.
Interacts with host DNA polymerase subunit POLA2.
Interacts with host single stranded DNA-binding protein RPA1.
Interacts with host TOP1; this interaction stimulates the enzymatic activity of TOP1.
UniRule annotationBy similarity3 PublicationsBinary interactionsi
P03116
With | #Exp. | IntAct |
---|---|---|
UBE2I [P63279] from Homo sapiens. | 2 | EBI-7015985,EBI-80168 |
Protein-protein interaction databases
DIPi | DIP-40903N |
ELMi | P03116 |
IntActi | P03116, 5 interactors |
MINTi | P03116 |
Structurei
Secondary structure
3D structure databases
SMRi | P03116 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P03116 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 407 – 557 | SF3 helicaseUniRule annotationAdd BLAST | 151 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 90 – 112 | DisorderedSequence analysisAdd BLAST | 23 | |
Regioni | 142 – 308 | DNA-binding regionUniRule annotationAdd BLAST | 167 | |
Regioni | 582 – 605 | DisorderedSequence analysisAdd BLAST | 24 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 84 – 86 | Nuclear localization signalUniRule annotation1 Publication | 3 | |
Motifi | 105 – 108 | Nuclear localization signal1 Publication | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 591 – 605 | Polar residuesSequence analysisAdd BLAST | 15 |
Sequence similaritiesi
Family and domain databases
Gene3Di | 1.10.10.510, 1 hit 3.40.50.300, 1 hit |
HAMAPi | MF_04000, PPV_E1, 1 hit |
InterProi | View protein in InterPro IPR001177, DNA_helicase_E1_C_Papillomavir IPR014000, DNA_helicase_E1_N_Papillomavir IPR014015, Helicase_SF3_DNA-vir IPR027417, P-loop_NTPase IPR016393, Rep_E1_papillomaV IPR037102, Znf_lg_T-Ag_D1_dom_sf |
Pfami | View protein in Pfam PF00519, PPV_E1_C, 1 hit PF00524, PPV_E1_N, 1 hit |
PIRSFi | PIRSF003383, Rep_E1_papillomaV, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51206, SF3_HELICASE_1, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MANDKGSNWD SGLGCSYLLT EAECESDKEN EEPGAGVELS VESDRYDSQD
60 70 80 90 100
EDFVDNASVF QGNHLEVFQA LEKKAGEEQI LNLKRKVLGS SQNSSGSEAS
110 120 130 140 150
ETPVKRRKSG AKRRLFAENE ANRVLTPLQV QGEGEGRQEL NEEQAISHLH
160 170 180 190 200
LQLVKSKNAT VFKLGLFKSL FLCSFHDITR LFKNDKTTNQ QWVLAVFGLA
210 220 230 240 250
EVFFEASFEL LKKQCSFLQM QKRSHEGGTC AVYLICFNTA KSRETVRNLM
260 270 280 290 300
ANTLNVREEC LMLQPAKIRG LSAALFWFKS SLSPATLKHG ALPEWIRAQT
310 320 330 340 350
TLNESLQTEK FDFGTMVQWA YDHKYAEESK IAYEYALAAG SDSNARAFLA
360 370 380 390 400
TNSQAKHVKD CATMVRHYLR AETQALSMPA YIKARCKLAT GEGSWKSILT
410 420 430 440 450
FFNYQNIELI TFINALKLWL KGIPKKNCLA FIGPPNTGKS MLCNSLIHFL
460 470 480 490 500
GGSVLSFANH KSHFWLASLA DTRAALVDDA THACWRYFDT YLRNALDGYP
510 520 530 540 550
VSIDRKHKAA VQIKAPPLLV TSNIDVQAED RYLYLHSRVQ TFRFEQPCTD
560 570 580 590 600
ESGEQPFNIT DADWKSFFVR LWGRLDLIDE EEDSEEDGDS MRTFTCSARN
TNAVD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X02346 Genomic DNA Translation: CAB46511.1 |
PIRi | A03663, W1WLEB |
RefSeqi | NP_056739.1, NC_001522.1 |
Genome annotation databases
GeneIDi | 1489019 |
KEGGi | vg:1489019 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X02346 Genomic DNA Translation: CAB46511.1 |
PIRi | A03663, W1WLEB |
RefSeqi | NP_056739.1, NC_001522.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1F08 | X-ray | 1.90 | A/B | 159-303 | [»] | |
1KSX | X-ray | 3.20 | A/B/E/F/I/J/M/N | 159-303 | [»] | |
1KSY | X-ray | 3.05 | A/B/C | 159-309 | [»] | |
2GXA | X-ray | 3.15 | A/B/C/D/E/F/G/H/I/J/K/L | 305-577 | [»] | |
2V9P | X-ray | 3.00 | A/B/C/D/E/F/G/H/I/J/K/L | 301-605 | [»] | |
5A9K | electron microscopy | 19.00 | A/B/C/D/E/F | 301-605 | [»] | |
SMRi | P03116 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-40903N |
ELMi | P03116 |
IntActi | P03116, 5 interactors |
MINTi | P03116 |
PTM databases
iPTMneti | P03116 |
Genome annotation databases
GeneIDi | 1489019 |
KEGGi | vg:1489019 |
Miscellaneous databases
EvolutionaryTracei | P03116 |
Family and domain databases
Gene3Di | 1.10.10.510, 1 hit 3.40.50.300, 1 hit |
HAMAPi | MF_04000, PPV_E1, 1 hit |
InterProi | View protein in InterPro IPR001177, DNA_helicase_E1_C_Papillomavir IPR014000, DNA_helicase_E1_N_Papillomavir IPR014015, Helicase_SF3_DNA-vir IPR027417, P-loop_NTPase IPR016393, Rep_E1_papillomaV IPR037102, Znf_lg_T-Ag_D1_dom_sf |
Pfami | View protein in Pfam PF00519, PPV_E1_C, 1 hit PF00524, PPV_E1_N, 1 hit |
PIRSFi | PIRSF003383, Rep_E1_papillomaV, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51206, SF3_HELICASE_1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | VE1_BPV1 | |
Accessioni | P03116Primary (citable) accession number: P03116 Secondary accession number(s): Q9WMH1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | February 23, 2022 | |
This is version 130 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families