UniProtKB - P03101 (VL1_HPV16)
Major capsid protein L1
L1
Functioni
Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with L2 proteins. Binds to heparan sulfate proteoglycans on cell surface of basal layer keratinocytes to provide initial virion attachment. This binding mediates a conformational change in the virus capsid that facilitates efficient infection. The virion enters the host cell via endocytosis. During virus trafficking, L1 protein dissociates from the viral DNA and the genomic DNA is released to the host nucleus. The virion assembly takes place within the cell nucleus. Encapsulates the genomic DNA together with protein L2.
UniRule annotation3 PublicationsMiscellaneous
GO - Molecular functioni
- structural molecule activity Source: UniProtKB-UniRule
GO - Biological processi
- endocytosis involved in viral entry into host cell Source: UniProtKB-KW
- virion attachment to host cell Source: UniProtKB-UniRule
Keywordsi
Biological process | Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus endocytosis by host, Virus entry into host cell |
Names & Taxonomyi
Protein namesi | Recommended name: Major capsid protein L1UniRule annotation |
Gene namesi | Name:L1UniRule annotation |
Organismi | Human papillomavirus type 16 |
Taxonomic identifieri | 333760 [NCBI] |
Taxonomic lineagei | Viruses › Monodnaviria › Shotokuvirae › Cossaviricota › Papovaviricetes › Zurhausenvirales › Papillomaviridae › Firstpapillomavirinae › Alphapapillomavirus › |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] |
Proteomesi |
|
Subcellular locationi
- Virion UniRule annotation
- Host nucleus UniRule annotation
Keywords - Cellular componenti
Capsid protein, Host nucleus, T=7 icosahedral capsid protein, VirionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000133500 | 1 – 505 | Major capsid protein L1Add BLAST | 505 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 175 | Interchain (with C-428)UniRule annotation1 Publication | ||
Disulfide bondi | 428 | Interchain (with C-175)UniRule annotation1 Publication |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Ubl conjugationProteomic databases
PRIDEi | P03101 |
Interactioni
Subunit structurei
Self-assembles into homopentamers. The capsid has an icosahedral symmetry and consists of 72 capsomers, with each capsomer being a pentamer of L1.
Interacts with the minor capsid protein L2; this interaction is necessary for viral genome encapsidation.
Interacts with protein E2; this interaction enhances E2-dependent replication and transcription activation (PubMed:25911730).
Interacts with host KPNA2; this interaction mediates the nuclear localization of L1 capsomers (PubMed:11971900).
Interacts with host ITGA6 (PubMed:11341777).
Interacts with host SDC1; this interaction promotes efficient infection of keratinocytes (PubMed:26289843).
UniRule annotation4 PublicationsBinary interactionsi
P03101
With | #Exp. | IntAct |
---|---|---|
KPNA2 [P52292] from Homo sapiens. | 3 | EBI-7362698,EBI-349938 |
KPNB1 [Q14974] from Homo sapiens. | 2 | EBI-7362698,EBI-286758 |
Protein-protein interaction databases
BioGRIDi | 4263561, 1 interactor |
IntActi | P03101, 3 interactors |
MINTi | P03101 |
Chemistry databases
BindingDBi | P03101 |
Structurei
Secondary structure
3D structure databases
SMRi | P03101 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P03101 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 480 – 505 | DisorderedSequence analysisAdd BLAST | 26 |
Sequence similaritiesi
Family and domain databases
Gene3Di | 2.60.175.20, 2 hits |
HAMAPi | MF_04002, PPV_L1, 1 hit |
InterProi | View protein in InterPro IPR002210, Capsid_L1_Papillomavir IPR036973, Capsid_L1_sf_Papillomavir IPR011222, dsDNA_vir_gr_I_capsid |
Pfami | View protein in Pfam PF00500, Late_protein_L1, 1 hit |
PRINTSi | PR00865, HPVCAPSIDL1 |
SUPFAMi | SSF88648, SSF88648, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSLWLPSEAT VYLPPVPVSK VVSTDEYVAR TNIYYHAGTS RLLAVGHPYF
60 70 80 90 100
PIKKPNNNKI LVPKVSGLQY RVFRIHLPDP NKFGFPDTSF YNPDTQRLVW
110 120 130 140 150
ACVGVEVGRG QPLGVGISGH PLLNKLDDTE NASAYAANAG VDNRECISMD
160 170 180 190 200
YKQTQLCLIG CKPPIGEHWG KGSPCTNVAV NPGDCPPLEL INTVIQDGDM
210 220 230 240 250
VDTGFGAMDF TTLQANKSEV PLDICTSICK YPDYIKMVSE PYGDSLFFYL
260 270 280 290 300
RREQMFVRHL FNRAGAVGEN VPDDLYIKGS GSTANLASSN YFPTPSGSMV
310 320 330 340 350
TSDAQIFNKP YWLQRAQGHN NGICWGNQLF VTVVDTTRST NMSLCAAIST
360 370 380 390 400
SETTYKNTNF KEYLRHGEEY DLQFIFQLCK ITLTADVMTY IHSMNSTILE
410 420 430 440 450
DWNFGLQPPP GGTLEDTYRF VTSQAIACQK HTPPAPKEDP LKKYTFWEVN
460 470 480 490 500
LKEKFSADLD QFPLGRKFLL QAGLKAKPKF TLGKRKATPT TSSTSTTAKR
KKRKL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 202 | D → H in AAA46943 (PubMed:2990099).Curated | 1 | |
Sequence conflicti | 266 | A → T in AAA46943 (PubMed:2990099).Curated | 1 | |
Sequence conflicti | 423 | Missing in AAA46943 (PubMed:2990099).Curated | 1 | |
Sequence conflicti | 439 | D → DD in AAA46943 (PubMed:2990099).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K02718 Genomic DNA Translation: AAA46943.1 AF001600 Genomic DNA Translation: AAC31789.1 AF125673 Genomic DNA Translation: AAD33259.1 AF536179 Genomic DNA Translation: AAQ10719.1 M96285 Genomic DNA Translation: AAA47024.1 A06331 Unassigned DNA Translation: CAA00546.1 |
PIRi | A03640, P1WLHS |
Similar proteinsi
Cross-referencesi
Web resourcesi
Virus Particle ExploreR db Icosahedral capsid structure |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K02718 Genomic DNA Translation: AAA46943.1 AF001600 Genomic DNA Translation: AAC31789.1 AF125673 Genomic DNA Translation: AAD33259.1 AF536179 Genomic DNA Translation: AAQ10719.1 M96285 Genomic DNA Translation: AAA47024.1 A06331 Unassigned DNA Translation: CAA00546.1 |
PIRi | A03640, P1WLHS |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1DZL | X-ray | 3.50 | A | 1-505 | [»] | |
3J6R | electron microscopy | 9.10 | A/B/C/D/E/F | 9-486 | [»] | |
3J7G | electron microscopy | 13.60 | A/B/C/D/E | 21-474 | [»] | |
3J8V | electron microscopy | 13.90 | A/B/C/D/E | 21-474 | [»] | |
3J8W | electron microscopy | 13.00 | A/B/C/D/E | 21-474 | [»] | |
3J8Z | electron microscopy | 14.00 | A/B/C/D/E | 21-474 | [»] | |
3JBA | electron microscopy | 12.00 | A/B/C/D/E/F | 9-486 | [»] | |
5KEP | electron microscopy | 4.30 | A/B/C/D/E/F | 3-485 | [»] | |
5KEQ | electron microscopy | 4.30 | A/B/C/D/E/F | 3-485 | [»] | |
6BSP | electron microscopy | 4.70 | C/D/E/F/G/H | 12-480 | [»] | |
6BT3 | electron microscopy | 4.70 | I/J/K/L/M/N | 1-503 | [»] | |
7CN2 | electron microscopy | 3.43 | A/B/C/D/E/F | 1-505 | [»] | |
7KZF | electron microscopy | 3.10 | A/B/C/D/E/F | 1-503 | [»] | |
SMRi | P03101 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263561, 1 interactor |
IntActi | P03101, 3 interactors |
MINTi | P03101 |
Chemistry databases
BindingDBi | P03101 |
ChEMBLi | CHEMBL3562172 |
Proteomic databases
PRIDEi | P03101 |
Protocols and materials databases
ABCDi | P03101, 5 sequenced antibodies |
Miscellaneous databases
EvolutionaryTracei | P03101 |
Family and domain databases
Gene3Di | 2.60.175.20, 2 hits |
HAMAPi | MF_04002, PPV_L1, 1 hit |
InterProi | View protein in InterPro IPR002210, Capsid_L1_Papillomavir IPR036973, Capsid_L1_sf_Papillomavir IPR011222, dsDNA_vir_gr_I_capsid |
Pfami | View protein in Pfam PF00500, Late_protein_L1, 1 hit |
PRINTSi | PR00865, HPVCAPSIDL1 |
SUPFAMi | SSF88648, SSF88648, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | VL1_HPV16 | |
Accessioni | P03101Primary (citable) accession number: P03101 Secondary accession number(s): O00530 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | January 20, 2016 | |
Last modified: | February 23, 2022 | |
This is version 131 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families