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Protein

Regulatory protein cro

Gene

cro

Organism
Escherichia phage lambda (Bacteriophage lambda)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an essential role in the switch from lysogenic to lytic state and in fine-tuning the lytic phase activation. Mediates the de-repression of the lytic promoters from repression by cI at an early stage of prophage induction. Turns down the expression of the transcriptional activator cII, a factor that initiates the expression of repressor cI, the major component promoting latency. Additionally, cro is able to directly bind to the promoter region of cI thereby preventing the transcription of cI repressor. These two mechanisms ultimately lead to a strong decrease in the amount of cI that will become unable to maintain the latent phase, allowing the transition to lytic growth. Low level of Cro also stimulates the lytic promoters while cI repressor is still present (PubMed:29158090). Cro-mediated up-regulation of pR and pL ultimately leads to its own increased expression and to a successful switch to lytic growth (PubMed:29158090). The second function assigned to Cro is the fine-tuning of the lytic phase activation. Indeed, cro initially diminishes the strength of activation by turning down the expression of lytic genes as well as its own expression.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi16 – 35H-T-H motifAdd BLAST20

GO - Molecular functioni

  • core promoter binding Source: CAFA
  • DNA binding Source: CAFA
  • protein homodimerization activity Source: CAFA

GO - Biological processi

  • latency-replication decision Source: UniProtKB
  • negative regulation of transcription by competitive promoter binding Source: CAFA
  • negative regulation of viral transcription Source: UniProtKB
  • release from viral latency Source: CAFA
  • response to UV Source: CAFA
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processLatency-replication decision, Transcription, Transcription regulation, Viral latency, Viral latency initiation and maintenance

Names & Taxonomyi

Protein namesi
Recommended name:
Regulatory protein cro
Gene namesi
Name:cro
Ordered Locus Names:lambdap57
OrganismiEscherichia phage lambda (Bacteriophage lambda)
Taxonomic identifieri10710 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridaeLambdavirus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000001711 Componenti: Genome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi33A → W: Loss of dimerization; when associated with D-58. 1 Publication1
Mutagenesisi58F → D: Loss of dimerization; when associated with W-33. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000775791 – 66Regulatory protein croAdd BLAST66

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Binds DNA as a homodimer.1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: CAFA

Protein-protein interaction databases

IntActiP03040, 1 interactor

Structurei

Secondary structure

166
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 6Combined sources4
Helixi7 – 14Combined sources8
Helixi16 – 23Combined sources8
Helixi27 – 36Combined sources10
Beta strandi39 – 44Combined sources6
Turni46 – 48Combined sources3
Beta strandi50 – 55Combined sources6
Beta strandi57 – 59Combined sources3

3D structure databases

DisProtiDP00741
ProteinModelPortaliP03040
SMRiP03040
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03040

Family & Domainsi

Phylogenomic databases

OrthoDBiVOG090001UA

Family and domain databases

Gene3Di3.30.240.10, 1 hit
InterProiView protein in InterPro
IPR000655 Cro
IPR038202 Cro_sf
IPR010982 Lambda_DNA-bd_dom_sf
PfamiView protein in Pfam
PF09048 Cro, 1 hit
PIRSFiPIRSF003217 Cro_protein, 1 hit
PRINTSiPR00030 HTHCRO
ProDomiView protein in ProDom or Entries sharing at least one domain
PD054632 HTH_lambda_Cro, 1 hit
SUPFAMiSSF47413 SSF47413, 1 hit

Sequencei

Sequence statusi: Complete.

P03040-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQRITLKDY AMRFGQTKTA KDLGVYQSAI NKAIHAGRKI FLTINADGSV
60
YAEEVKPFPS NKKTTA
Length:66
Mass (Da):7,363
Last modified:July 21, 1986 - v1
Checksum:i233A02DA0873E4D4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02459 Genomic DNA Translation: AAA96582.1
PIRiB94614 RCBPL
RefSeqiNP_040629.1, NC_001416.1

Genome annotation databases

GeneIDi2703467
KEGGivg:2703467

Similar proteinsi

Entry informationi

Entry nameiRCRO_LAMBD
AccessioniPrimary (citable) accession number: P03040
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: July 18, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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