UniProtKB - P03040 (RCRO_LAMBD)
Protein
Regulatory protein cro
Gene
cro
Organism
Escherichia phage lambda (Bacteriophage lambda)
Status
Functioni
Plays an essential role in the switch from lysogenic to lytic state and in fine-tuning the lytic phase activation. Mediates the de-repression of the lytic promoters from repression by cI at an early stage of prophage induction. Turns down the expression of the transcriptional activator cII, a factor that initiates the expression of repressor cI, the major component promoting latency. Additionally, cro is able to directly bind to the promoter region of cI thereby preventing the transcription of cI repressor. These two mechanisms ultimately lead to a strong decrease in the amount of cI that will become unable to maintain the latent phase, allowing the transition to lytic growth. Low level of Cro also stimulates the lytic promoters while cI repressor is still present (PubMed:29158090). Cro-mediated up-regulation of pR and pL ultimately leads to its own increased expression and to a successful switch to lytic growth (PubMed:29158090). The second function assigned to Cro is the fine-tuning of the lytic phase activation. Indeed, cro initially diminishes the strength of activation by turning down the expression of lytic genes as well as its own expression.4 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 16 – 35 | H-T-H motifAdd BLAST | 20 |
GO - Molecular functioni
- core promoter sequence-specific DNA binding Source: CAFA
- DNA binding Source: CAFA
- protein homodimerization activity Source: CAFA
GO - Biological processi
- latency-replication decision Source: UniProtKB
- negative regulation of transcription by competitive promoter binding Source: CAFA
- negative regulation of viral transcription Source: UniProtKB
- release from viral latency Source: CAFA
- response to UV Source: CAFA
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Latency-replication decision, Transcription, Transcription regulation, Viral latency, Viral latency initiation and maintenance |
Names & Taxonomyi
Protein namesi | Recommended name: Regulatory protein cro |
Gene namesi | Name:cro Ordered Locus Names:lambdap57 |
Organismi | Escherichia phage lambda (Bacteriophage lambda) |
Taxonomic identifieri | 10710 [NCBI] |
Taxonomic lineagei | Viruses › Duplodnaviria › Heunggongvirae › Uroviricota › Caudoviricetes › Caudovirales › Siphoviridae › Lambdavirus |
Virus hosti | Escherichia coli [TaxID: 562] |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 33 | A → W: Loss of dimerization; when associated with D-58. 1 Publication | 1 | |
Mutagenesisi | 58 | F → D: Loss of dimerization; when associated with W-33. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000077579 | 1 – 66 | Regulatory protein croAdd BLAST | 66 |
Interactioni
Subunit structurei
Binds DNA as a homodimer.
1 PublicationGO - Molecular functioni
- protein homodimerization activity Source: CAFA
Protein-protein interaction databases
IntActi | P03040, 1 interactor |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P03040 |
SMRi | P03040 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P03040 |
Family & Domainsi
Family and domain databases
DisProti | DP00741 |
Gene3Di | 3.30.240.10, 1 hit |
InterProi | View protein in InterPro IPR000655, Cro IPR038202, Cro_sf IPR010982, Lambda_DNA-bd_dom_sf |
Pfami | View protein in Pfam PF09048, Cro, 1 hit |
PIRSFi | PIRSF003217, Cro_protein, 1 hit |
PRINTSi | PR00030, HTHCRO |
SUPFAMi | SSF47413, SSF47413, 1 hit |
i Sequence
Sequence statusi: Complete.
P03040-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEQRITLKDY AMRFGQTKTA KDLGVYQSAI NKAIHAGRKI FLTINADGSV
60
YAEEVKPFPS NKKTTA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02459 Genomic DNA Translation: AAA96582.1 |
PIRi | B94614, RCBPL |
RefSeqi | NP_040629.1, NC_001416.1 |
Genome annotation databases
GeneIDi | 2703466 2703467 |
KEGGi | vg:2703467 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02459 Genomic DNA Translation: AAA96582.1 |
PIRi | B94614, RCBPL |
RefSeqi | NP_040629.1, NC_001416.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1COP | NMR | - | D/E | 1-66 | [»] | |
1D1L | X-ray | 2.10 | A | 1-61 | [»] | |
1D1M | X-ray | 2.05 | A/B | 1-60 | [»] | |
1ORC | X-ray | 1.54 | A | 1-66 | [»] | |
2A63 | NMR | - | A | 1-66 | [»] | |
2ECS | X-ray | 1.40 | A/B | 1-66 | [»] | |
2ORC | NMR | - | A | 1-66 | [»] | |
2OVG | X-ray | 1.35 | A | 1-66 | [»] | |
3ORC | X-ray | 3.00 | A | 2-61 | [»] | |
4CRO | X-ray | 3.90 | A/B/C/D/E/F | 1-66 | [»] | |
5CRO | X-ray | 2.30 | A/B/C/O | 1-66 | [»] | |
6CRO | X-ray | 3.00 | A | 2-61 | [»] | |
BMRBi | P03040 | |||||
SMRi | P03040 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P03040, 1 interactor |
Genome annotation databases
GeneIDi | 2703466 2703467 |
KEGGi | vg:2703467 |
Miscellaneous databases
EvolutionaryTracei | P03040 |
Family and domain databases
DisProti | DP00741 |
Gene3Di | 3.30.240.10, 1 hit |
InterProi | View protein in InterPro IPR000655, Cro IPR038202, Cro_sf IPR010982, Lambda_DNA-bd_dom_sf |
Pfami | View protein in Pfam PF09048, Cro, 1 hit |
PIRSFi | PIRSF003217, Cro_protein, 1 hit |
PRINTSi | PR00030, HTHCRO |
SUPFAMi | SSF47413, SSF47413, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RCRO_LAMBD | |
Accessioni | P03040Primary (citable) accession number: P03040 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | April 7, 2021 | |
This is version 142 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references