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Protein

DNA helicase II

Gene

uvrD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

A helicase with DNA-dependent ATPase activity (PubMed:8419285). Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand. Initiates unwinding more efficiently from a nicked substrate than ds duplex DNA (PubMed:8419285). Involved in the post-incision events of nucleotide excision repair and methyl-directed mismatch repair, and probably also in repair of alkylated DNA (Probable).1 Publication1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Kineticsi

  1. KM=0.05 mM for ATP1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei284ATPBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi32 – 37ATPPROSITE-ProRule annotation6

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionDNA-binding, Helicase, Hydrolase
    Biological processDNA damage, DNA repair, DNA replication, SOS response
    LigandATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11064-MONOMER
    BRENDAi3.6.4.12 2026

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA helicase II (EC:3.6.4.12)
    Gene namesi
    Name:uvrD
    Synonyms:mutU, pdeB, rad, recL
    Ordered Locus Names:b3813, JW3786
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG11064 uvrD

    Subcellular locationi

    GO - Cellular componenti

    Pathology & Biotechi

    Disruption phenotypei

    Strongly sensitive to UV, ciprofloxacin (CFX), and azidothymidine (AZT) in single deletion mutants, radA-uvrD double deletions are more sensitive yet. Adding recF mutations almost completely suppresses AZT and partially suppresses UV and CFX sensitivity, suggesting RadA processes a class of intermediates that accumulate in uvrD mutants (PubMed:25484163).1 Publication

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi30G → D in uvrD252, UV sensitive, significant loss of DNA-dependent ATPase, helicase activity requires higher ATP and MgCl(2), nearly inactive on 96 bp dsDNA. KM for ATP rises to 1.2 mM. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001020721 – 720DNA helicase IIAdd BLAST720

    Proteomic databases

    PaxDbiP03018
    PRIDEiP03018

    Interactioni

    Binary interactionsi

    Protein-protein interaction databases

    BioGridi4263340, 265 interactors
    DIPiDIP-11103N
    IntActiP03018, 39 interactors
    STRINGi316385.ECDH10B_4005

    Structurei

    Secondary structure

    1720
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    ProteinModelPortaliP03018
    SMRiP03018
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP03018

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini8 – 286UvrD-like helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST279
    Domaini287 – 564UvrD-like helicase C-terminalPROSITE-ProRule annotationAdd BLAST278

    Sequence similaritiesi

    Belongs to the helicase family. UvrD subfamily.Curated

    Phylogenomic databases

    eggNOGiENOG4105C4R Bacteria
    COG0210 LUCA
    HOGENOMiHOG000033016
    InParanoidiP03018
    KOiK03657
    PhylomeDBiP03018

    Family and domain databases

    Gene3Di1.10.10.160, 1 hit
    InterProiView protein in InterPro
    IPR013986 DExx_box_DNA_helicase_dom_sf
    IPR005753 DNA_helicase_ATP-dep_UvrD
    IPR014017 DNA_helicase_UvrD-like_C
    IPR000212 DNA_helicase_UvrD/REP
    IPR027417 P-loop_NTPase
    IPR014016 UvrD-like_ATP-bd
    IPR034739 UvrD/AddA_N
    PANTHERiPTHR11070 PTHR11070, 1 hit
    PfamiView protein in Pfam
    PF00580 UvrD-helicase, 1 hit
    PF13361 UvrD_C, 1 hit
    SUPFAMiSSF52540 SSF52540, 1 hit
    TIGRFAMsiTIGR01075 uvrD, 1 hit
    PROSITEiView protein in PROSITE
    PS51198 UVRD_HELICASE_ATP_BIND, 1 hit
    PS51217 UVRD_HELICASE_CTER, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P03018-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MDVSYLLDSL NDKQREAVAA PRSNLLVLAG AGSGKTRVLV HRIAWLMSVE
    60 70 80 90 100
    NCSPYSIMAV TFTNKAAAEM RHRIGQLMGT SQGGMWVGTF HGLAHRLLRA
    110 120 130 140 150
    HHMDANLPQD FQILDSEDQL RLLKRLIKAM NLDEKQWPPR QAMWYINSQK
    160 170 180 190 200
    DEGLRPHHIQ SYGNPVEQTW QKVYQAYQEA CDRAGLVDFA ELLLRAHELW
    210 220 230 240 250
    LNKPHILQHY RERFTNILVD EFQDTNNIQY AWIRLLAGDT GKVMIVGDDD
    260 270 280 290 300
    QSIYGWRGAQ VENIQRFLND FPGAETIRLE QNYRSTSNIL SAANALIENN
    310 320 330 340 350
    NGRLGKKLWT DGADGEPISL YCAFNELDEA RFVVNRIKTW QDNGGALAEC
    360 370 380 390 400
    AILYRSNAQS RVLEEALLQA SMPYRIYGGM RFFERQEIKD ALSYLRLIAN
    410 420 430 440 450
    RNDDAAFERV VNTPTRGIGD RTLDVVRQTS RDRQLTLWQA CRELLQEKAL
    460 470 480 490 500
    AGRAASALQR FMELIDALAQ ETADMPLHVQ TDRVIKDSGL RTMYEQEKGE
    510 520 530 540 550
    KGQTRIENLE ELVTATRQFS YNEEDEDLMP LQAFLSHAAL EAGEGQADTW
    560 570 580 590 600
    QDAVQLMTLH SAKGLEFPQV FIVGMEEGMF PSQMSLDEGG RLEEERRLAY
    610 620 630 640 650
    VGVTRAMQKL TLTYAETRRL YGKEVYHRPS RFIGELPEEC VEEVRLRATV
    660 670 680 690 700
    SRPVSHQRMG TPMVENDSGY KLGQRVRHAK FGEGTIVNME GSGEHSRLQV
    710 720
    AFQGQGIKWL VAAYARLESV
    Length:720
    Mass (Da):81,990
    Last modified:April 1, 1988 - v1
    Checksum:iFA68E7267C77B49A
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti224D → N in CAA25321 (PubMed:6379604).Curated1
    Sequence conflicti291 – 293SAA → NAR in CAA25321 (PubMed:6379604).Curated3
    Sequence conflicti330A → T in CAA25321 (PubMed:6379604).Curated1
    Sequence conflicti540L → V in CAA25321 (PubMed:6379604).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X00738 Genomic DNA Translation: CAA25321.1
    D00069 Genomic DNA Translation: BAA00048.1
    X04037 Genomic DNA Translation: CAA27671.1
    M87049 Genomic DNA Translation: AAA67609.1
    U00096 Genomic DNA Translation: AAC76816.1
    AP009048 Genomic DNA Translation: BAE77487.1
    X00225 Genomic DNA Translation: CAA25043.1
    M38257 Genomic DNA Translation: AAA24765.1
    PIRiF65185 HJECD2
    RefSeqiNP_418258.1, NC_000913.3
    WP_000383406.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC76816; AAC76816; b3813
    BAE77487; BAE77487; BAE77487
    GeneIDi948347
    KEGGiecj:JW3786
    eco:b3813
    PATRICifig|511145.12.peg.3929

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X00738 Genomic DNA Translation: CAA25321.1
    D00069 Genomic DNA Translation: BAA00048.1
    X04037 Genomic DNA Translation: CAA27671.1
    M87049 Genomic DNA Translation: AAA67609.1
    U00096 Genomic DNA Translation: AAC76816.1
    AP009048 Genomic DNA Translation: BAE77487.1
    X00225 Genomic DNA Translation: CAA25043.1
    M38257 Genomic DNA Translation: AAA24765.1
    PIRiF65185 HJECD2
    RefSeqiNP_418258.1, NC_000913.3
    WP_000383406.1, NZ_LN832404.1

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2IS1X-ray2.90A/B1-680[»]
    2IS2X-ray3.00A/B1-680[»]
    2IS4X-ray2.60A/B1-680[»]
    2IS6X-ray2.20A/B1-680[»]
    3LFUX-ray1.80A1-647[»]
    ProteinModelPortaliP03018
    SMRiP03018
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4263340, 265 interactors
    DIPiDIP-11103N
    IntActiP03018, 39 interactors
    STRINGi316385.ECDH10B_4005

    Proteomic databases

    PaxDbiP03018
    PRIDEiP03018

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC76816; AAC76816; b3813
    BAE77487; BAE77487; BAE77487
    GeneIDi948347
    KEGGiecj:JW3786
    eco:b3813
    PATRICifig|511145.12.peg.3929

    Organism-specific databases

    EchoBASEiEB1057
    EcoGeneiEG11064 uvrD

    Phylogenomic databases

    eggNOGiENOG4105C4R Bacteria
    COG0210 LUCA
    HOGENOMiHOG000033016
    InParanoidiP03018
    KOiK03657
    PhylomeDBiP03018

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11064-MONOMER
    BRENDAi3.6.4.12 2026

    Miscellaneous databases

    EvolutionaryTraceiP03018
    PROiPR:P03018

    Family and domain databases

    Gene3Di1.10.10.160, 1 hit
    InterProiView protein in InterPro
    IPR013986 DExx_box_DNA_helicase_dom_sf
    IPR005753 DNA_helicase_ATP-dep_UvrD
    IPR014017 DNA_helicase_UvrD-like_C
    IPR000212 DNA_helicase_UvrD/REP
    IPR027417 P-loop_NTPase
    IPR014016 UvrD-like_ATP-bd
    IPR034739 UvrD/AddA_N
    PANTHERiPTHR11070 PTHR11070, 1 hit
    PfamiView protein in Pfam
    PF00580 UvrD-helicase, 1 hit
    PF13361 UvrD_C, 1 hit
    SUPFAMiSSF52540 SSF52540, 1 hit
    TIGRFAMsiTIGR01075 uvrD, 1 hit
    PROSITEiView protein in PROSITE
    PS51198 UVRD_HELICASE_ATP_BIND, 1 hit
    PS51217 UVRD_HELICASE_CTER, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiUVRD_ECOLI
    AccessioniPrimary (citable) accession number: P03018
    Secondary accession number(s): P76758, Q2M8B9, Q47709
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: April 1, 1988
    Last modified: November 7, 2018
    This is version 168 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
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    Main funding by: National Institutes of Health

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