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Protein

Transcription factor IIIA

Gene

gtf3a

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in ribosomal large subunit biogenesis (By similarity). Acts as both a positive transcription factor for 5S RNA genes and a specific RNA binding protein that complexes with 5S RNA in oocytes to form the 7S ribonucleoprotein storage particle. May play an essential role in the developmental change in 5S RNA gene expression. Interacts with the internal control region (ICR) of approximately 50 bases within the 5S RNA genes, is required for correct transcription of these genes by RNA polymerase III (PubMed:2253880, PubMed:1538401, PubMed:1762917). Also binds the transcribed 5S RNA's (By similarity).By similarity3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri35 – 59C2H2-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri65 – 89C2H2-type 2PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri95 – 120C2H2-type 3PROSITE-ProRule annotationAdd BLAST26
Zinc fingeri127 – 151C2H2-type 4PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri157 – 181C2H2-type 5PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri184 – 210C2H2-type 6PROSITE-ProRule annotationAdd BLAST27
Zinc fingeri214 – 236C2H2-type 7PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri243 – 268C2H2-type 8PROSITE-ProRule annotationAdd BLAST26
Zinc fingeri274 – 298C2H2-type 9PROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, RNA-binding
Biological processRibosome biogenesis, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor IIIA
Short name:
TFIIIA
Alternative name(s):
S-TFIIIA/O-TFIIIA
Gene namesi
Name:gtf3a
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6252591 gtf3a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi38S → E or A: Abolishes phosphorylation by CK2. 1 Publication1
Mutagenesisi42C → S: Loss of DNA binding. 1 Publication1
Mutagenesisi55H → L: Inhibition of specific DNA binding. 1 Publication1
Mutagenesisi91G → S: Loss of DNA binding. 1 Publication1
Mutagenesisi93K → E: Loss of DNA binding. 1 Publication1
Mutagenesisi134C → S: No effect. 1 Publication1
Mutagenesisi186C → S: No effect. 1 Publication1
Mutagenesisi336S → E or A: Slightly decreases phosphorylation by CK2. 1 Publication1
Mutagenesisi350S → E or A: No effect on phosphorylation by CK2. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000416321 – 366Transcription factor IIIAAdd BLAST366

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei38Phosphoserine; by CK21 Publication1
Modified residuei336Phosphoserine; by CK2; in vitro1 Publication1

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP03001

PTM databases

iPTMnetiP03001

Expressioni

Tissue specificityi

Synthesized in oocytes and, in much lower levels, in somatic cells.

Developmental stagei

The levels follow the transcriptional activity of oocyte type 5S RNA genes during embryogenesis, present in very high levels in maturing oocytes when oocyte type 5S genes are being expressed, and in much lower levels in somatic cells where the oocyte type genes are not expressed.

Structurei

Secondary structure

1366
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP03001
SMRiP03001
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03001

Family & Domainsi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri35 – 59C2H2-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri65 – 89C2H2-type 2PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri95 – 120C2H2-type 3PROSITE-ProRule annotationAdd BLAST26
Zinc fingeri127 – 151C2H2-type 4PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri157 – 181C2H2-type 5PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri184 – 210C2H2-type 6PROSITE-ProRule annotationAdd BLAST27
Zinc fingeri214 – 236C2H2-type 7PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri243 – 268C2H2-type 8PROSITE-ProRule annotationAdd BLAST26
Zinc fingeri274 – 298C2H2-type 9PROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

HOVERGENiHBG017915
KOiK09191

Family and domain databases

InterProiView protein in InterPro
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 9 hits
SUPFAMiSSF57667 SSF57667, 6 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 8 hits
PS50157 ZINC_FINGER_C2H2_2, 7 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket
Isoform Somatic (identifier: P03001-1) [UniParc]FASTAAdd to basket
Also known as: S-TFIIIA

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAAKVASTSS EEAEGSLVTE GEMGEKALPV VYKRYICSFA DCGAAYNKNW
60 70 80 90 100
KLQAHLCKHT GEKPFPCKEE GCEKGFTSLH HLTRHSLTHT GEKNFTCDSD
110 120 130 140 150
GCDLRFTTKA NMKKHFNRFH NIKICVYVCH FENCGKAFKK HNQLKVHQFS
160 170 180 190 200
HTQQLPYECP HEGCDKRFSL PSRLKRHEKV HAGYPCKKDD SCSFVGKTWT
210 220 230 240 250
LYLKHVAECH QDLAVCDVCN RKFRHKDYLR DHQKTHEKER TVYLCPRDGC
260 270 280 290 300
DRSYTTAFNL RSHIQSFHEE QRPFVCEHAG CGKCFAMKKS LERHSVVHDP
310 320 330 340 350
EKRKLKEKCP RPKRSLASRL TGYIPPKSKE KNASVSGTEK TDSLVKNKPS
360
GTETNGSLVL DKLTIQ
Length:366
Mass (Da):41,911
Last modified:July 15, 1998 - v2
Checksum:i29237E710292FA24
GO
Isoform Oocyte (identifier: P03001-2) [UniParc]FASTAAdd to basket
Also known as: O-TFIIIA

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: Missing.

Show »
Length:344
Mass (Da):39,746
Checksum:i8ACFE717EA8A1322
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4K → T in CAA33786 (PubMed:2744458).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti96T → K. 1
Natural varianti319R → C. 1
Natural varianti335V → I. 1
Natural varianti356G → D. 1
Natural varianti365I → L. 1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0189621 – 22Missing in isoform Oocyte. CuratedAdd BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02938 mRNA Translation: AAA49967.1
X03681
, X03735, X03736, X03737, X03738, X03739 Genomic DNA Translation: CAB51745.1
X15785 Genomic DNA Translation: CAA33786.1
PIRiA35916
A90857 TWXL3
RefSeqiNP_001081328.1, NM_001087859.1 [P03001-2]
UniGeneiXl.860

Genome annotation databases

GeneIDi397777
KEGGixla:397777

Keywords - Coding sequence diversityi

Alternative initiation, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiTF3A_XENLA
AccessioniPrimary (citable) accession number: P03001
Secondary accession number(s): Q91856
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 15, 1998
Last modified: February 28, 2018
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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