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Protein

Immunoglobulin G-binding protein A

Gene

spa

Organism
Staphylococcus aureus (strain NCTC 8325)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Important immunodiagnostic reagent because of its ability to bind the Fc fragment of a wide range of mammalian immunoglobulins.

GO - Molecular functioni

GO - Biological processi

  • pathogenesis Source: CACAO

Keywordsi

Molecular functionIgG-binding protein

Enzyme and pathway databases

BioCyciSAUR93061:G1G5Y-64-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Immunoglobulin G-binding protein A
Short name:
IgG-binding protein A
Alternative name(s):
Staphylococcal protein A
Gene namesi
Name:spa
Ordered Locus Names:SAOUHSC_00069
OrganismiStaphylococcus aureus (strain NCTC 8325)
Taxonomic identifieri93061 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000008816 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell wall, Secreted

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 36Sequence analysisAdd BLAST36
ChainiPRO_000000565337 – 485Immunoglobulin G-binding protein AAdd BLAST449
PropeptideiPRO_0000005654486 – 516Removed by sortasePROSITE-ProRule annotationAdd BLAST31

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei485Pentaglycyl murein peptidoglycan amidated threoninePROSITE-ProRule annotation1

Keywords - PTMi

Peptidoglycan-anchor

Proteomic databases

PRIDEiP02976

Expressioni

Inductioni

More protein is secreted in a secG or double secG/secY2 mutant (at protein level).1 Publication

Interactioni

Protein-protein interaction databases

IntActiP02976, 1 interactor
MINTiP02976
STRINGi93061.SAOUHSC_00069

Structurei

Secondary structure

1516
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi104 – 113Combined sources10
Beta strandi115 – 117Combined sources3
Helixi119 – 131Combined sources13
Helixi133 – 135Combined sources3
Helixi136 – 149Combined sources14
Helixi218 – 229Combined sources12
Helixi235 – 247Combined sources13
Helixi249 – 251Combined sources3
Helixi252 – 265Combined sources14

3D structure databases

ProteinModelPortaliP02976
SMRiP02976
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02976

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati37 – 88B 1Add BLAST52
Repeati96 – 149B 2Add BLAST54
Repeati154 – 207B 3Add BLAST54
Repeati212 – 265B 4Add BLAST54
Repeati266 – 323B 5Add BLAST58
Repeati333 – 3402-18
Repeati341 – 3482-28
Repeati349 – 3562-38
Repeati357 – 3642-48
Repeati365 – 3722-58
Repeati373 – 3802-68
Repeati381 – 3882-78
Repeati389 – 3962-88
Repeati397 – 4042-98
Repeati405 – 4122-108
Repeati413 – 4202-118
Domaini421 – 465LysMPROSITE-ProRule annotationAdd BLAST45

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni333 – 42011 X 8 AA approximate tandem repeatsAdd BLAST88

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi482 – 486LPXTG sorting signalPROSITE-ProRule annotation5

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG4106I9N Bacteria
ENOG410XXBQ LUCA
HOGENOMiHOG000279707
KOiK14196
OMAiAQQNKFN

Family and domain databases

CDDicd00118 LysM, 1 hit
Gene3Di3.10.350.10, 1 hit
InterProiView protein in InterPro
IPR019948 Gram-positive_anchor
IPR009063 Ig/albumin-bd_sf
IPR018392 LysM_dom
IPR036779 LysM_dom_sf
IPR005038 Octapeptide
IPR003132 Protein_A_Ig-bd
IPR005877 YSIRK_signal_dom
PfamiView protein in Pfam
PF02216 B, 5 hits
PF00746 Gram_pos_anchor, 1 hit
PF01476 LysM, 1 hit
PF03373 Octapeptide, 12 hits
PF04650 YSIRK_signal, 1 hit
SMARTiView protein in SMART
SM00257 LysM, 1 hit
SUPFAMiSSF46997 SSF46997, 5 hits
TIGRFAMsiTIGR01168 YSIRK_signal, 1 hit
PROSITEiView protein in PROSITE
PS50847 GRAM_POS_ANCHORING, 1 hit
PS51782 LYSM, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02976-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKKNIYSIR KLGVGIASVT LGTLLISGGV TPAANAAQHD EAQQNAFYQV
60 70 80 90 100
LNMPNLNADQ RNGFIQSLKD DPSQSANVLG EAQKLNDSQA PKADAQQNNF
110 120 130 140 150
NKDQQSAFYE ILNMPNLNEA QRNGFIQSLK DDPSQSTNVL GEAKKLNESQ
160 170 180 190 200
APKADNNFNK EQQNAFYEIL NMPNLNEEQR NGFIQSLKDD PSQSANLLSE
210 220 230 240 250
AKKLNESQAP KADNKFNKEQ QNAFYEILHL PNLNEEQRNG FIQSLKDDPS
260 270 280 290 300
QSANLLAEAK KLNDAQAPKA DNKFNKEQQN AFYEILHLPN LTEEQRNGFI
310 320 330 340 350
QSLKDDPSVS KEILAEAKKL NDAQAPKEED NNKPGKEDNN KPGKEDNNKP
360 370 380 390 400
GKEDNNKPGK EDNNKPGKED GNKPGKEDNK KPGKEDGNKP GKEDNKKPGK
410 420 430 440 450
EDGNKPGKED GNKPGKEDGN GVHVVKPGDT VNDIAKANGT TADKIAADNK
460 470 480 490 500
LADKNMIKPG QELVVDKKQP ANHADANKAQ ALPETGEENP FIGTTVFGGL
510
SLALGAALLA GRRREL
Length:516
Mass (Da):56,437
Last modified:July 11, 2006 - v3
Checksum:iC6FEFFF9E54BE1F0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti101N → D in CAA24596 (PubMed:6338496).Curated1
Sequence conflicti370D → DNNKPGKED in AAA26676 (PubMed:6319407).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01786 Genomic DNA Translation: AAA26676.1
CP000253 Genomic DNA Translation: ABD29253.1
V01287 Genomic DNA Translation: CAA24596.1
PIRiA58531 QVSAA
RefSeqiWP_000728764.1, NC_007795.1
YP_498670.1, NC_007795.1

Genome annotation databases

EnsemblBacteriaiABD29253; ABD29253; SAOUHSC_00069
GeneIDi3919448
KEGGisao:SAOUHSC_00069
PATRICifig|93061.5.peg.59

Entry informationi

Entry nameiSPA_STAA8
AccessioniPrimary (citable) accession number: P02976
Secondary accession number(s): Q2G1N8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 11, 2006
Last modified: July 18, 2018
This is version 163 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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