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Protein

Immunoglobulin G-binding protein A

Gene

spa

Organism
Staphylococcus aureus (strain NCTC 8325)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Avidly binds the Fc region of immunoglobulins (Ig) from most mammalian species, in a non-specific aggregation reaction that can lead to host tissue damage (PubMed:4163007, PubMed:2938951) (Probable). Also binds the Fab region of Igs, in a region remote from the Ig light chain and the heavy chain constant region, i.e. not a typical Ig-antigen recognition (PubMed:10805799).1 Publication3 Publications

GO - Molecular functioni

GO - Biological processi

  • pathogenesis Source: CACAO

Keywordsi

Molecular functionIgG-binding protein
Biological processVirulence

Enzyme and pathway databases

BioCyciSAUR93061:G1G5Y-64-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Immunoglobulin G-binding protein A
Short name:
IgG-binding protein A
Alternative name(s):
Staphylococcal protein A
Short name:
SpA
Gene namesi
Name:spa
Ordered Locus Names:SAOUHSC_00069
OrganismiStaphylococcus aureus (strain NCTC 8325)
Taxonomic identifieri93061 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000008816 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell wall, Secreted

Pathology & Biotechi

Biotechnological usei

Important immunodiagnostic reagent because of its ability to bind the Fab and Fc fragments of a wide range of mammalian immunoglobulins.Curated

Disruption phenotypei

Decreased virulence in mice (PubMed:3679545). Bacteria do not bind IgG (PubMed:12700270). No visible phenotype during growth in liquid culture (PubMed:22447609).3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi7 – 10Missing : Wild-type anchoring to cell wall, decreased signal peptide processing rate, decreased amount of protein on cell surface, overall IgG-binding capacity is slightly decreased. 1 Publication4
Mutagenesisi15G → A: Altered processing of signal peptide, precursor accumulates in membrane. More aberrant processing; when associated with 18-A or deletion of 7-10. 1 Publication1
Mutagenesisi18S → A: Altered processing of signal peptide, precursor accumulates in membrane and cytoplasm. More aberrant processing; when associated with 15-A or deletion of 7-10. 1 Publication1
Mutagenesisi478K → E: Wild-type anchoring to cell wall. 1 Publication1
Mutagenesisi482 – 516Missing : Protein secreted, no longer anchored to cell wall. 1 PublicationAdd BLAST35
Mutagenesisi482 – 487Missing : Protein mis-sorted, found in cytoplasm, membrane and cell wall, cells bind Ig poorly. 1 Publication6
Mutagenesisi483P → N: Protein mis-sorted, found in cytoplasm, membrane and cell wall, cells bind Ig poorly. 1 Publication1
Mutagenesisi485T → A: Wild-type anchoring to cell wall. 1 Publication1
Mutagenesisi488 – 516Missing : Protein secreted, no longer anchored to cell wall. 1 PublicationAdd BLAST29
Mutagenesisi512 – 516Missing : Protein secreted, no longer anchored to cell wall. 1 Publication5
Mutagenesisi514 – 516Missing : Protein secreted, no longer anchored to cell wall. 1 Publication3
Mutagenesisi515 – 516Missing : Protein mis-sorted, found in cytoplasm, membrane and cell wall, cells bind Ig poorly. 1 Publication2
Mutagenesisi516L → C: Protein secreted, no longer anchored to cell wall. 1 Publication1
Mutagenesisi516Missing : Wild-type anchoring to cell wall. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 362 PublicationsAdd BLAST36
ChainiPRO_000000565337 – 485Immunoglobulin G-binding protein AAdd BLAST449
PropeptideiPRO_0000005654486 – 516Removed by sortase APROSITE-ProRule annotation1 Publication1 PublicationAdd BLAST31

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei485Pentaglycyl murein peptidoglycan amidated threoninePROSITE-ProRule annotation2 Publications1

Keywords - PTMi

Peptidoglycan-anchor

Proteomic databases

PRIDEiP02976

Expressioni

Inductioni

More protein is secreted in a secG or double secG/secY2 mutant (at protein level).1 Publication

Interactioni

Protein-protein interaction databases

MINTiP02976
STRINGi93061.SAOUHSC_00069

Structurei

Secondary structure

1516
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP02976
SMRiP02976
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02976

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati37 – 92Immunoglobulin-binding region E1 PublicationAdd BLAST56
Repeati93 – 153Immunoglobulin-binding region D1 PublicationAdd BLAST61
Repeati154 – 211Immunoglobulin-binding region A1 PublicationAdd BLAST58
Repeati212 – 269Immunoglobulin-binding region B1 PublicationAdd BLAST58
Repeati270 – 327Immunoglobulin-binding region C1 PublicationAdd BLAST58
Repeati333 – 3402-18
Repeati341 – 3482-28
Repeati349 – 3562-38
Repeati357 – 3642-48
Repeati365 – 3722-58
Repeati373 – 3802-68
Repeati381 – 3882-78
Repeati389 – 3962-88
Repeati397 – 4042-98
Repeati405 – 4122-108
Repeati413 – 4202-118
Domaini421 – 465LysMPROSITE-ProRule annotationAdd BLAST45

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni333 – 42011 X 8 AA approximate tandem repeatsAdd BLAST88

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi7 – 18YSIRK-G/S signaling motif1 PublicationAdd BLAST12
Motifi482 – 486LPXTG sorting signalPROSITE-ProRule annotation1 Publication5

Domaini

Each of the immunoglobulin-binding region repeats can bind the Fc region of an immunoglobulin (PubMed:2938951). Cell wall anchoring is confered by the LPXTG motif and following sequences (residues 482-516) which are necessary and sufficient to target other proteins to the cell wall (PubMed:1638631). The YSIRK-G/S motif in the signal sequence plays a role in the secretion efficiency for SpA, it may have a specialized recognition mode (Probable).1 Publication2 Publications

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG4106I9N Bacteria
ENOG410XXBQ LUCA
HOGENOMiHOG000279707
KOiK14196
OMAiAQQNKFN

Family and domain databases

CDDicd00118 LysM, 1 hit
Gene3Di3.10.350.10, 1 hit
InterProiView protein in InterPro
IPR019948 Gram-positive_anchor
IPR009063 Ig/albumin-bd_sf
IPR018392 LysM_dom
IPR036779 LysM_dom_sf
IPR005038 Octapeptide
IPR003132 Protein_A_Ig-bd
IPR005877 YSIRK_signal_dom
PfamiView protein in Pfam
PF02216 B, 5 hits
PF00746 Gram_pos_anchor, 1 hit
PF01476 LysM, 1 hit
PF03373 Octapeptide, 12 hits
PF04650 YSIRK_signal, 1 hit
SMARTiView protein in SMART
SM00257 LysM, 1 hit
SUPFAMiSSF46997 SSF46997, 5 hits
TIGRFAMsiTIGR01168 YSIRK_signal, 1 hit
PROSITEiView protein in PROSITE
PS50847 GRAM_POS_ANCHORING, 1 hit
PS51782 LYSM, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02976-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKKKNIYSIR KLGVGIASVT LGTLLISGGV TPAANAAQHD EAQQNAFYQV
60 70 80 90 100
LNMPNLNADQ RNGFIQSLKD DPSQSANVLG EAQKLNDSQA PKADAQQNNF
110 120 130 140 150
NKDQQSAFYE ILNMPNLNEA QRNGFIQSLK DDPSQSTNVL GEAKKLNESQ
160 170 180 190 200
APKADNNFNK EQQNAFYEIL NMPNLNEEQR NGFIQSLKDD PSQSANLLSE
210 220 230 240 250
AKKLNESQAP KADNKFNKEQ QNAFYEILHL PNLNEEQRNG FIQSLKDDPS
260 270 280 290 300
QSANLLAEAK KLNDAQAPKA DNKFNKEQQN AFYEILHLPN LTEEQRNGFI
310 320 330 340 350
QSLKDDPSVS KEILAEAKKL NDAQAPKEED NNKPGKEDNN KPGKEDNNKP
360 370 380 390 400
GKEDNNKPGK EDNNKPGKED GNKPGKEDNK KPGKEDGNKP GKEDNKKPGK
410 420 430 440 450
EDGNKPGKED GNKPGKEDGN GVHVVKPGDT VNDIAKANGT TADKIAADNK
460 470 480 490 500
LADKNMIKPG QELVVDKKQP ANHADANKAQ ALPETGEENP FIGTTVFGGL
510
SLALGAALLA GRRREL
Length:516
Mass (Da):56,437
Last modified:July 11, 2006 - v3
Checksum:iC6FEFFF9E54BE1F0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti101N → D in CAA24596 (PubMed:6338496).Curated1
Sequence conflicti370D → DNNKPGKED in AAA26676 (PubMed:6319407).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01786 Genomic DNA Translation: AAA26676.1
CP000253 Genomic DNA Translation: ABD29253.1
V01287 Genomic DNA Translation: CAA24596.1
PIRiA58531 QVSAA
RefSeqiWP_000728764.1, NZ_LS483365.1
YP_498670.1, NC_007795.1

Genome annotation databases

EnsemblBacteriaiABD29253; ABD29253; SAOUHSC_00069
GeneIDi3919448
KEGGisao:SAOUHSC_00069
PATRICifig|93061.5.peg.59

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01786 Genomic DNA Translation: AAA26676.1
CP000253 Genomic DNA Translation: ABD29253.1
V01287 Genomic DNA Translation: CAA24596.1
PIRiA58531 QVSAA
RefSeqiWP_000728764.1, NZ_LS483365.1
YP_498670.1, NC_007795.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DEEX-ray2.70G/H100-153[»]
4Y4YX-ray3.00A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d158-211[»]
A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d219-354[»]
A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d236-459[»]
4Y5ZX-ray2.950/1/2/3/4/5/6/7/A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V158-211[»]
5U4YX-ray2.50C/D215-268[»]
ProteinModelPortaliP02976
SMRiP02976
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiP02976
STRINGi93061.SAOUHSC_00069

Proteomic databases

PRIDEiP02976

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD29253; ABD29253; SAOUHSC_00069
GeneIDi3919448
KEGGisao:SAOUHSC_00069
PATRICifig|93061.5.peg.59

Phylogenomic databases

eggNOGiENOG4106I9N Bacteria
ENOG410XXBQ LUCA
HOGENOMiHOG000279707
KOiK14196
OMAiAQQNKFN

Enzyme and pathway databases

BioCyciSAUR93061:G1G5Y-64-MONOMER

Miscellaneous databases

EvolutionaryTraceiP02976
PROiPR:P02976

Family and domain databases

CDDicd00118 LysM, 1 hit
Gene3Di3.10.350.10, 1 hit
InterProiView protein in InterPro
IPR019948 Gram-positive_anchor
IPR009063 Ig/albumin-bd_sf
IPR018392 LysM_dom
IPR036779 LysM_dom_sf
IPR005038 Octapeptide
IPR003132 Protein_A_Ig-bd
IPR005877 YSIRK_signal_dom
PfamiView protein in Pfam
PF02216 B, 5 hits
PF00746 Gram_pos_anchor, 1 hit
PF01476 LysM, 1 hit
PF03373 Octapeptide, 12 hits
PF04650 YSIRK_signal, 1 hit
SMARTiView protein in SMART
SM00257 LysM, 1 hit
SUPFAMiSSF46997 SSF46997, 5 hits
TIGRFAMsiTIGR01168 YSIRK_signal, 1 hit
PROSITEiView protein in PROSITE
PS50847 GRAM_POS_ANCHORING, 1 hit
PS51782 LYSM, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSPA_STAA8
AccessioniPrimary (citable) accession number: P02976
Secondary accession number(s): Q2G1N8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 11, 2006
Last modified: October 10, 2018
This is version 165 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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