UniProtKB - P02974 (FMM1_NEIGO)

BLAST

>sp|P02974|FMM1_NEIGO Fimbrial protein OS=Neisseria gonorrhoeae OX=485 GN=pilE1 PE=1 SV=2
MNTLQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQVSEAILLAEGQKSAVTEYYLN
HGKWPENNTSAGVASPPSDIKGKYVKEVEVKNGVVTATMLSSGVNNEIKGKKLSLWARRE
NGSVKWFCGQPVTRTDDDTVADAKDGKEIDTKHLPSTCRDNFDAK

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History
Entry version 115 (25 Oct 2017)
Sequence version 2 (01 Nov 1995)
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Protein

Fimbrial protein

Gene

pilE1

Organism

Neisseria gonorrhoeae

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

This protein is the predominant Neisseria surface antigen, which allows adhesion of the bacterium to various host cells.

Caution

In PubMed:413571 it is said that 50% of the peptides have N-methyl-Phe and 50% begin with Thr-9. N-terminal methylation produces preview during Edman degradation, which makes this appear to happen when the peptide is completely N-terminal methylated.Curated

GO - Biological processⁱ

cell adhesion Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Cell adhesion

Biological process

Cell adhesion

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Fimbrial protein Alternative name(s): MS11 antigen Pilin
Gene namesⁱ	Name:pilE1
Organismⁱ	Neisseria gonorrhoeae
Taxonomic identifierⁱ	485 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Subcellular locationⁱ

Fimbrium

GO - Cellular componentⁱ

pilus Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Fimbrium

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	75	S → A: Alters the morphology of fibers. 1 Publication		1

Chemistry databases

Drug and drug target database More...DrugBankⁱ	DB06838 methyl L-phenylalaninate DB04522 Phosphonoserine

DrugBankⁱ

DB06838 methyl L-phenylalaninate
DB04522 Phosphonoserine

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
PropeptideⁱPRO_0000024154	1 – 7	2 Publications		7
ChainⁱPRO_0000024155	8 – 165	Fimbrial proteinAdd BLAST		158

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	8	N-methylphenylalanine3 Publications	1
Glycosylationⁱ	70	O-linked (DADDGlc) serine3 Publications	1
Modified residueⁱ	75	O-(2-aminoethylphosphoryl)serine; alternate3 Publications	1
Modified residueⁱ	75	O-(2-cholinephosphoryl)serine; alternate3 Publications	1
Modified residueⁱ	75	Phosphoserine; alternate3 Publications	1
Modified residueⁱ	101	O-(sn-1-glycerophosphoryl)serine; partial1 Publication	1
Disulfide bondⁱ	128 ↔ 158	1 Publication

Post-translational modificationⁱ

The O-linked glycan identified as Gal-GlcNAc disaccharide in PubMed:7477282 and PubMed:10048019 is now identified as either a hexosyl-diacetamidotrideoxyhexoside (DATDHex) by mass spectrometry in PubMed:15249686, or alpha-D-galactopyranosyl-(1->3)-2,4-diacetamido-2,4-dideoxy-beta-D-glucopyranoside (DADDGlc) by X-ray diffraction in PubMed:16949362. It is not clear whether there is a chemical difference in the glycosylation of the two derivatives of strain MS11 used in these experiments, or not.2 Publications

In some MS11 derivative strains, Ser-75 is modified to O-(2-aminoethylphosphoryl)serine, and in some other derivatives that can be secondarily modified to O-(2-cholinephosphoryl)serine by N-methylation.2 Publications

Keywords - PTMⁱ

Disulfide bond, Glycoprotein, Methylation, Phosphoprotein

PTM databases

iPTMnetⁱ	P02974

iPTMnetⁱ

P02974

Miscellaneous databases

PMAP-CutDBⁱ	P02974

PMAP-CutDBⁱ

P02974

Interactionⁱ

Subunit structureⁱ

The pili are polar flexible filaments of about 5.4 nanometers diameter and 2.5 micrometers average length; they consist of only a single polypeptide chain arranged in a helical configuration of five subunits per turn in the assembled pilus.

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	10 – 27	Combined sources	18
Turnⁱ	28 – 30	Combined sources	3
Helixⁱ	31 – 47	Combined sources	17
Helixⁱ	51 – 61	Combined sources	11
Helixⁱ	68 – 71	Combined sources	4
Helixⁱ	77 – 79	Combined sources	3
Beta strandⁱ	83 – 91	Combined sources	9
Beta strandⁱ	94 – 99	Combined sources	6
Beta strandⁱ	101 – 104	Combined sources	4
Beta strandⁱ	106 – 108	Combined sources	3
Beta strandⁱ	112 – 119	Combined sources	8
Beta strandⁱ	121 – 130	Combined sources	10
Beta strandⁱ	132 – 136	Combined sources	5
Beta strandⁱ	139 – 142	Combined sources	4
Helixⁱ	151 – 153	Combined sources	3
Beta strandⁱ	156 – 158	Combined sources	3

Show more details

3D structure databases

ProteinModelPortalⁱ	P02974
SMRⁱ	P02974
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

P02974

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the N-Me-Phe pilin family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG4108ZUW Bacteria COG4969 LUCA

eggNOGⁱ

ENOG4108ZUW Bacteria
COG4969 LUCA

Family and domain databases

InterProⁱ	View protein in InterPro IPR012902 N_methyl_site IPR001082 Pilin
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF07963 N_methyl, 1 hit PF00114 Pilin, 1 hit
TIGRFAMsⁱ	TIGR02532 IV_pilin_GFxxxE, 1 hit
PROSITEⁱ	View protein in PROSITE PS00409 PROKAR_NTER_METHYL, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P02974-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MNTLQKGFTL IELMIVIAIV GILAAVALPA YQDYTARAQV SEAILLAEGQ 
        60         70         80         90        100
KSAVTEYYLN HGKWPENNTS AGVASPPSDI KGKYVKEVEV KNGVVTATML 
       110        120        130        140        150
SSGVNNEIKG KKLSLWARRE NGSVKWFCGQ PVTRTDDDTV ADAKDGKEID 
       160 
TKHLPSTCRD NFDAK

Length:165

Mass (Da):17,944

Last modified:November 1, 1995 - v2

Checksum:ⁱBCD3723C11AF5B4C

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	161 – 162	NF → KAS (PubMed:6148752).Curated	2
Sequence conflictⁱ	161 – 162	NF → KAS (PubMed:2872674).Curated	2
Sequence conflictⁱ	161 – 162	NF → KAS (PubMed:1671354).Curated	2
Sequence conflictⁱ	161 – 162	NF → KAS (PubMed:1348857).Curated	2
Sequence conflictⁱ	161 – 162	NF → KAS (PubMed:6143785).Curated	2

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	K02078 Genomic DNA Translation: AAA25466.1 M13222 mRNA Translation: AAA25468.1
PIRⁱ	A94007 YQNHG

Protein	Similar proteins		Species	Score	Length	Source
P02974	Pilin E (Fragment)		NEIGO		38	UniRef100_P02974
UPI00019643D0		NEIGO		158

Protein	Similar proteins		Species	Score	Length	Source
P02974	Pilin E (Fragment)		NEIGO		38	UniRef90_P02974
PilE protein		NEIME		166
Pilin		NEIGO		168
Pilin		NEIGO		168
UPI00019643D0		NEIGO		158
+2

Protein	Similar proteins		Species	Score	Length	Source
P02974	Fimbrial protein P9-2		NEIGO		167	UniRef50_P02974
Fimbrial protein	)	NEIMB		170
Fimbrial protein		NEIMA		170
PilE protein		NEIME		166
Truncated pilin		Neisseria meningitidis alpha275		168
+520

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	K02078 Genomic DNA Translation: AAA25466.1 M13222 mRNA Translation: AAA25468.1
PIRⁱ	A94007 YQNHG

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1AY2	X-ray	2.60	A	9-165	[»]
	2HI2	X-ray	2.30	A	8-165	[»]
	2HIL	electron microscopy	12.50	A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R	8-165	[»]
	2PIL	X-ray	2.60	A	8-165	[»]
	5VXX	electron microscopy	5.10	A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U	8-165	[»]
ProteinModelPortalⁱ	P02974
SMRⁱ	P02974
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Chemistry databases

DrugBankⁱ	DB06838 methyl L-phenylalaninate DB04522 Phosphonoserine

DrugBankⁱ

DB06838 methyl L-phenylalaninate
DB04522 Phosphonoserine

PTM databases

iPTMnetⁱ	P02974

iPTMnetⁱ

P02974

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Phylogenomic databases

eggNOGⁱ	ENOG4108ZUW Bacteria COG4969 LUCA

eggNOGⁱ

ENOG4108ZUW Bacteria
COG4969 LUCA

Miscellaneous databases

EvolutionaryTraceⁱ	P02974
PMAP-CutDBⁱ	P02974

Family and domain databases

InterProⁱ	View protein in InterPro IPR012902 N_methyl_site IPR001082 Pilin
Pfamⁱ	View protein in Pfam PF07963 N_methyl, 1 hit PF00114 Pilin, 1 hit
TIGRFAMsⁱ	TIGR02532 IV_pilin_GFxxxE, 1 hit
PROSITEⁱ	View protein in PROSITE PS00409 PROKAR_NTER_METHYL, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	FMM1_NEIGO
Accessionⁱ	P02974Primary (citable) accession number: P02974
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
	Last sequence update:	November 1, 1995
	Last modified:	October 25, 2017
	This is version 115 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

UniProtKB

UniParc

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UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P02974 (FMM1_NEIGO)

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Fimbrial protein

pilE1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Caution

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

PTM databases

Miscellaneous databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Chemistry databases

PTM databases

Protocols and materials databases

Phylogenomic databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ