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Protein

Fimbrial protein

Gene

pilE1

Organism
Neisseria gonorrhoeae
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This protein is the predominant Neisseria surface antigen, which allows adhesion of the bacterium to various host cells.

Caution

In PubMed:413571 it is said that 50% of the peptides have N-methyl-Phe and 50% begin with Thr-9. N-terminal methylation produces preview during Edman degradation, which makes this appear to happen when the peptide is completely N-terminal methylated.Curated

GO - Biological processi

Keywordsi

Biological processCell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
Fimbrial protein
Alternative name(s):
MS11 antigen
Pilin
Gene namesi
Name:pilE1
OrganismiNeisseria gonorrhoeae
Taxonomic identifieri485 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

Subcellular locationi

GO - Cellular componenti

  • pilus Source: UniProtKB-SubCell

Keywords - Cellular componenti

Fimbrium

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi75S → A: Alters the morphology of fibers. 1 Publication1

Chemistry databases

DrugBankiDB06838 methyl L-phenylalaninate
DB04522 Phosphonoserine

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000241541 – 72 Publications7
ChainiPRO_00000241558 – 165Fimbrial proteinAdd BLAST158

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei8N-methylphenylalanine3 Publications1
Glycosylationi70O-linked (DADDGlc) serine3 Publications1
Modified residuei75O-(2-aminoethylphosphoryl)serine; alternate3 Publications1
Modified residuei75O-(2-cholinephosphoryl)serine; alternate3 Publications1
Modified residuei75Phosphoserine; alternate3 Publications1
Modified residuei101O-(sn-1-glycerophosphoryl)serine; partial1 Publication1
Disulfide bondi128 ↔ 1581 Publication

Post-translational modificationi

The O-linked glycan identified as Gal-GlcNAc disaccharide in PubMed:7477282 and PubMed:10048019 is now identified as either a hexosyl-diacetamidotrideoxyhexoside (DATDHex) by mass spectrometry in PubMed:15249686, or alpha-D-galactopyranosyl-(1->3)-2,4-diacetamido-2,4-dideoxy-beta-D-glucopyranoside (DADDGlc) by X-ray diffraction in PubMed:16949362. It is not clear whether there is a chemical difference in the glycosylation of the two derivatives of strain MS11 used in these experiments, or not.2 Publications
In some MS11 derivative strains, Ser-75 is modified to O-(2-aminoethylphosphoryl)serine, and in some other derivatives that can be secondarily modified to O-(2-cholinephosphoryl)serine by N-methylation.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein, Methylation, Phosphoprotein

PTM databases

iPTMnetiP02974

Miscellaneous databases

PMAP-CutDBiP02974

Interactioni

Subunit structurei

The pili are polar flexible filaments of about 5.4 nanometers diameter and 2.5 micrometers average length; they consist of only a single polypeptide chain arranged in a helical configuration of five subunits per turn in the assembled pilus.

Structurei

Secondary structure

1165
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP02974
SMRiP02974
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02974

Family & Domainsi

Sequence similaritiesi

Belongs to the N-Me-Phe pilin family.Curated

Phylogenomic databases

eggNOGiENOG4108ZUW Bacteria
COG4969 LUCA

Family and domain databases

InterProiView protein in InterPro
IPR012902 N_methyl_site
IPR001082 Pilin
PfamiView protein in Pfam
PF07963 N_methyl, 1 hit
PF00114 Pilin, 1 hit
TIGRFAMsiTIGR02532 IV_pilin_GFxxxE, 1 hit
PROSITEiView protein in PROSITE
PS00409 PROKAR_NTER_METHYL, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02974-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNTLQKGFTL IELMIVIAIV GILAAVALPA YQDYTARAQV SEAILLAEGQ
60 70 80 90 100
KSAVTEYYLN HGKWPENNTS AGVASPPSDI KGKYVKEVEV KNGVVTATML
110 120 130 140 150
SSGVNNEIKG KKLSLWARRE NGSVKWFCGQ PVTRTDDDTV ADAKDGKEID
160
TKHLPSTCRD NFDAK
Length:165
Mass (Da):17,944
Last modified:November 1, 1995 - v2
Checksum:iBCD3723C11AF5B4C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti161 – 162NF → KAS (PubMed:6148752).Curated2
Sequence conflicti161 – 162NF → KAS (PubMed:2872674).Curated2
Sequence conflicti161 – 162NF → KAS (PubMed:1671354).Curated2
Sequence conflicti161 – 162NF → KAS (PubMed:1348857).Curated2
Sequence conflicti161 – 162NF → KAS (PubMed:6143785).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02078 Genomic DNA Translation: AAA25466.1
M13222 mRNA Translation: AAA25468.1
PIRiA94007 YQNHG

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02078 Genomic DNA Translation: AAA25466.1
M13222 mRNA Translation: AAA25468.1
PIRiA94007 YQNHG

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AY2X-ray2.60A9-165[»]
2HI2X-ray2.30A8-165[»]
2HILelectron microscopy12.50A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R8-165[»]
2PILX-ray2.60A8-165[»]
5VXXelectron microscopy5.10A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U8-165[»]
ProteinModelPortaliP02974
SMRiP02974
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

DrugBankiDB06838 methyl L-phenylalaninate
DB04522 Phosphonoserine

PTM databases

iPTMnetiP02974

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4108ZUW Bacteria
COG4969 LUCA

Miscellaneous databases

EvolutionaryTraceiP02974
PMAP-CutDBiP02974

Family and domain databases

InterProiView protein in InterPro
IPR012902 N_methyl_site
IPR001082 Pilin
PfamiView protein in Pfam
PF07963 N_methyl, 1 hit
PF00114 Pilin, 1 hit
TIGRFAMsiTIGR02532 IV_pilin_GFxxxE, 1 hit
PROSITEiView protein in PROSITE
PS00409 PROKAR_NTER_METHYL, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiFMM1_NEIGO
AccessioniPrimary (citable) accession number: P02974
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 1, 1995
Last modified: October 25, 2017
This is version 115 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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