UniProtKB - P02942 (MCP1_ECOLI)
Protein
Methyl-accepting chemotaxis protein I
Gene
tsr
Organism
Escherichia coli (strain K12)
Status
Functioni
Receptor for the attractant L-serine and related amino acids. Is also responsible for chemotaxis away from a wide range of repellents, including leucine, indole, and weak acids.
Chemotactic-signal transducers respond to changes in the concentration of attractants and repellents in the environment, transduce a signal from the outside to the inside of the cell, and facilitate sensory adaptation through the variation of the level of methylation. Attractants increase the level of methylation while repellents decrease the level of methylation, the methyl groups are added by the methyltransferase CheR and removed by the methylesterase CheB.
GO - Molecular functioni
- identical protein binding Source: CAFA
- transmembrane signaling receptor activity Source: GO_Central
GO - Biological processi
- cell motility Source: CACAO
- cellular response to amino acid stimulus Source: CAFA
- chemotaxis Source: CACAO
- detection of chemical stimulus Source: CAFA
- receptor clustering Source: CAFA
- regulation of bacterial-type flagellum-dependent cell motility Source: CAFA
- regulation of chemotaxis Source: CAFA
- regulation of protein histidine kinase activity Source: CAFA
- signal complex assembly Source: CAFA
- signal transduction Source: CACAO
Keywordsi
Molecular function | Transducer |
Biological process | Chemotaxis |
Enzyme and pathway databases
BioCyci | EcoCyc:TSR-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Methyl-accepting chemotaxis protein IShort name: MCP-I Alternative name(s): Serine chemoreceptor protein |
Gene namesi | Name:tsr Synonyms:cheD Ordered Locus Names:b4355, JW4318 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane 2 Publications; Multi-pass membrane protein 2 Publications Note: Found predominantly at cell poles.
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 6 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 7 – 30 | HelicalSequence analysisAdd BLAST | 24 | |
Topological domaini | 31 – 190 | PeriplasmicSequence analysisAdd BLAST | 160 | |
Transmembranei | 191 – 210 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 211 – 551 | CytoplasmicSequence analysisAdd BLAST | 341 |
GO - Cellular componenti
- integral component of plasma membrane Source: CAFA
- methyl accepting chemotaxis protein complex Source: CAFA
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000110537 | 1 – 551 | Methyl-accepting chemotaxis protein IAdd BLAST | 551 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 297 | Glutamate methyl ester (Gln)2 Publications | 1 | |
Modified residuei | 304 | Glutamate methyl ester (Glu)2 Publications | 1 | |
Modified residuei | 311 | Glutamate methyl ester (Gln)2 Publications | 1 | |
Modified residuei | 493 | Glutamate methyl ester (Glu)2 Publications | 1 | |
Modified residuei | 502 | Glutamate methyl ester (Glu)1 Publication | 1 |
Keywords - PTMi
MethylationProteomic databases
PaxDbi | P02942 |
PRIDEi | P02942 |
Interactioni
GO - Molecular functioni
- identical protein binding Source: CAFA
Protein-protein interaction databases
BioGRIDi | 4261744, 203 interactors 853162, 1 interactor |
DIPi | DIP-11046N |
IntActi | P02942, 4 interactors |
MINTi | P02942 |
STRINGi | 511145.b4355 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P02942 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P02942 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 216 – 268 | HAMPPROSITE-ProRule annotationAdd BLAST | 53 | |
Domaini | 273 – 502 | Methyl-accepting transducerPROSITE-ProRule annotationAdd BLAST | 230 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 64 – 73 | The 3 Arg may form a positively charged pocket, which binds the alpha-carboxyl group of the attractant AA | 10 |
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0840, Bacteria |
HOGENOMi | CLU_000445_107_16_6 |
InParanoidi | P02942 |
PhylomeDBi | P02942 |
Family and domain databases
CDDi | cd06225, HAMP, 1 hit cd00181, Tar_Tsr_LBD, 1 hit |
InterProi | View protein in InterPro IPR035440, 4HB_MCP_dom_sf IPR004090, Chemotax_Me-accpt_rcpt IPR004091, Chemotax_Me-accpt_rcpt_Me-site IPR003660, HAMP_dom IPR004089, MCPsignal_dom IPR003122, Tar_rcpt_lig-bd |
Pfami | View protein in Pfam PF00672, HAMP, 1 hit PF00015, MCPsignal, 1 hit PF02203, TarH, 1 hit |
PRINTSi | PR00260, CHEMTRNSDUCR |
SMARTi | View protein in SMART SM00304, HAMP, 1 hit SM00283, MA, 1 hit SM00319, TarH, 1 hit |
SUPFAMi | SSF47170, SSF47170, 1 hit |
PROSITEi | View protein in PROSITE PS00538, CHEMOTAXIS_TRANSDUC_1, 1 hit PS50111, CHEMOTAXIS_TRANSDUC_2, 1 hit PS50885, HAMP, 1 hit |
i Sequence
Sequence statusi: Complete.
P02942-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLKRIKIVTS LLLVLAVFGL LQLTSGGLFF NALKNDKENF TVLQTIRQQQ
60 70 80 90 100
STLNGSWVAL LQTRNTLNRA GIRYMMDQNN IGSGSTVAEL MESASISLKQ
110 120 130 140 150
AEKNWADYEA LPRDPRQSTA AAAEIKRNYD IYHNALAELI QLLGAGKINE
160 170 180 190 200
FFDQPTQGYQ DGFEKQYVAY MEQNDRLHDI AVSDNNASYS QAMWILVGVM
210 220 230 240 250
IVVLAVIFAV WFGIKASLVA PMNRLIDSIR HIAGGDLVKP IEVDGSNEMG
260 270 280 290 300
QLAESLRHMQ GELMRTVGDV RNGANAIYSG ASEIATGNND LSSRTEQQAA
310 320 330 340 350
SLEETAASME QLTATVKQNA ENARQASHLA LSASETAQRG GKVVDNVVQT
360 370 380 390 400
MRDISTSSQK IADIISVIDG IAFQTNILAL NAAVEAARAG EQGRGFAVVA
410 420 430 440 450
GEVRNLAQRS AQAAREIKSL IEDSVGKVDV GSTLVESAGE TMAEIVSAVT
460 470 480 490 500
RVTDIMGEIA SASDEQSRGI DQVGLAVAEM DRVTQQNAAL VEESAAAAAA
510 520 530 540 550
LEEQASRLTE AVAVFRIQQQ QRETSAVVKT VTPAAPRKMA VADSEENWET
F
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V00373 Genomic DNA Translation: CAA23676.1 Sequence problems. U14003 Genomic DNA Translation: AAA97252.1 U00096 Genomic DNA Translation: AAC77311.1 AP009048 Genomic DNA Translation: BAE78345.1 S56952 Genomic DNA Translation: AAB25802.1 |
PIRi | E65250, QRECS |
RefSeqi | NP_418775.1, NC_000913.3 WP_000919536.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC77311; AAC77311; b4355 BAE78345; BAE78345; BAE78345 |
GeneIDi | 49586240 948884 |
KEGGi | ecj:JW4318 eco:b4355 |
PATRICi | fig|1411691.4.peg.2331 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V00373 Genomic DNA Translation: CAA23676.1 Sequence problems. U14003 Genomic DNA Translation: AAA97252.1 U00096 Genomic DNA Translation: AAC77311.1 AP009048 Genomic DNA Translation: BAE78345.1 S56952 Genomic DNA Translation: AAB25802.1 |
PIRi | E65250, QRECS |
RefSeqi | NP_418775.1, NC_000913.3 WP_000919536.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1QU7 | X-ray | 2.60 | A/B | 294-520 | [»] | |
2D4U | X-ray | 1.95 | A/B | 25-190 | [»] | |
3ATP | X-ray | 2.50 | A/B | 25-190 | [»] | |
3ZX6 | X-ray | 2.65 | A/B | 264-551 | [»] | |
6S1K | electron microscopy | 8.38 | E/F/G/H/I/J/K/L/M/N/O/P | 1-551 | [»] | |
SMRi | P02942 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261744, 203 interactors 853162, 1 interactor |
DIPi | DIP-11046N |
IntActi | P02942, 4 interactors |
MINTi | P02942 |
STRINGi | 511145.b4355 |
Proteomic databases
PaxDbi | P02942 |
PRIDEi | P02942 |
Genome annotation databases
EnsemblBacteriai | AAC77311; AAC77311; b4355 BAE78345; BAE78345; BAE78345 |
GeneIDi | 49586240 948884 |
KEGGi | ecj:JW4318 eco:b4355 |
PATRICi | fig|1411691.4.peg.2331 |
Organism-specific databases
EchoBASEi | EB1027 |
Phylogenomic databases
eggNOGi | COG0840, Bacteria |
HOGENOMi | CLU_000445_107_16_6 |
InParanoidi | P02942 |
PhylomeDBi | P02942 |
Enzyme and pathway databases
BioCyci | EcoCyc:TSR-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P02942 |
PROi | PR:P02942 |
Family and domain databases
CDDi | cd06225, HAMP, 1 hit cd00181, Tar_Tsr_LBD, 1 hit |
InterProi | View protein in InterPro IPR035440, 4HB_MCP_dom_sf IPR004090, Chemotax_Me-accpt_rcpt IPR004091, Chemotax_Me-accpt_rcpt_Me-site IPR003660, HAMP_dom IPR004089, MCPsignal_dom IPR003122, Tar_rcpt_lig-bd |
Pfami | View protein in Pfam PF00672, HAMP, 1 hit PF00015, MCPsignal, 1 hit PF02203, TarH, 1 hit |
PRINTSi | PR00260, CHEMTRNSDUCR |
SMARTi | View protein in SMART SM00304, HAMP, 1 hit SM00283, MA, 1 hit SM00319, TarH, 1 hit |
SUPFAMi | SSF47170, SSF47170, 1 hit |
PROSITEi | View protein in PROSITE PS00538, CHEMOTAXIS_TRANSDUC_1, 1 hit PS50111, CHEMOTAXIS_TRANSDUC_2, 1 hit PS50885, HAMP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MCP1_ECOLI | |
Accessioni | P02942Primary (citable) accession number: P02942 Secondary accession number(s): P76817, Q2M5W1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 1, 1993 | |
Last modified: | December 2, 2020 | |
This is version 187 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references