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Protein

Outer membrane protein F

Gene

ompF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Forms pores that allow passive diffusion of small molecules across the outer membrane. It is also a receptor for the bacteriophage T2.1 Publication

GO - Molecular functioni

  • colicin transmembrane transporter activity Source: EcoCyc
  • disordered domain specific binding Source: CAFA
  • drug transmembrane transporter activity Source: EcoCyc
  • identical protein binding Source: CAFA
  • ion channel activity Source: CAFA
  • ion transmembrane transporter activity Source: EcoCyc
  • porin activity Source: EcoliWiki
  • protein N-terminus binding Source: CAFA

GO - Biological processi

Keywordsi

Molecular functionPorin
Biological processIon transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG10671-MONOMER
MetaCyc:EG10671-MONOMER

Protein family/group databases

TCDBi1.B.1.1.1 the general bacterial porin (gbp) family

Names & Taxonomyi

Protein namesi
Recommended name:
Outer membrane protein F
Alternative name(s):
Outer membrane protein 1A
Outer membrane protein B
Outer membrane protein IA
Porin OmpF
Gene namesi
Name:ompF
Synonyms:cmlB, coa, cry, tolF
Ordered Locus Names:b0929, JW0912
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10671 ompF

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei23 – 28Beta stranded6
Topological domaini29Periplasmic1
Transmembranei30 – 45Beta strandedAdd BLAST16
Topological domaini46 – 60ExtracellularAdd BLAST15
Transmembranei61 – 73Beta strandedAdd BLAST13
Topological domaini74 – 75Periplasmic2
Transmembranei76 – 88Beta strandedAdd BLAST13
Topological domaini89 – 104ExtracellularAdd BLAST16
Transmembranei105 – 113Beta stranded9
Topological domaini114 – 115Periplasmic2
Transmembranei116 – 122Beta stranded7
Topological domaini123 – 156ExtracellularAdd BLAST34
Transmembranei157 – 163Beta stranded7
Topological domaini164 – 171Periplasmic8
Transmembranei172 – 181Beta stranded10
Topological domaini182 – 193ExtracellularAdd BLAST12
Transmembranei194 – 204Beta strandedAdd BLAST11
Topological domaini205Periplasmic1
Transmembranei206 – 217Beta strandedAdd BLAST12
Topological domaini218 – 232ExtracellularAdd BLAST15
Transmembranei233 – 244Beta strandedAdd BLAST12
Topological domaini245Periplasmic1
Transmembranei246 – 257Beta strandedAdd BLAST12
Topological domaini258 – 274ExtracellularAdd BLAST17
Transmembranei275 – 287Beta strandedAdd BLAST13
Topological domaini288 – 289Periplasmic2
Transmembranei290 – 303Beta strandedAdd BLAST14
Topological domaini304 – 313Extracellular10
Transmembranei314 – 325Beta strandedAdd BLAST12
Topological domaini326 – 327Periplasmic2
Transmembranei328 – 337Beta stranded10
Topological domaini338 – 352ExtracellularAdd BLAST15
Transmembranei353 – 362Beta stranded10

GO - Cellular componenti

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Leads to decreased susceptibility to a number of hydrophilic antibiotics including ampicillin, cefoxitin and tetracycline.1 Publication

Chemistry databases

DrugBankiDB04233 (Hydroxyethyloxy)Tri(Ethyloxy)Octane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 223 PublicationsAdd BLAST22
ChainiPRO_000002523723 – 362Outer membrane protein FAdd BLAST340

Proteomic databases

EPDiP02931
PaxDbiP02931
PRIDEiP02931

2D gel databases

SWISS-2DPAGEiP02931

Expressioni

Inductioni

Levels of OmpF protein decrease in a lon/ycgE double disruption (at protein level).1 Publication

Interactioni

Subunit structurei

Homotrimer.1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4260018, 306 interactors
DIPiDIP-10398N
IntActiP02931, 9 interactors
MINTiP02931
STRINGi316385.ECDH10B_0999

Structurei

Secondary structure

1362
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP02931
SMRiP02931
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02931

Family & Domainsi

Sequence similaritiesi

Belongs to the Gram-negative porin family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4105FCK Bacteria
COG3203 LUCA
HOGENOMiHOG000272406
InParanoidiP02931
KOiK09476
OMAiDAIGWTD
PhylomeDBiP02931

Family and domain databases

CDDicd00342 gram_neg_porins, 1 hit
Gene3Di2.40.160.10, 1 hit
InterProiView protein in InterPro
IPR033900 Gram_neg_porin_domain
IPR023614 Porin_dom_sf
IPR001897 Porin_gammaproteobac
IPR001702 Porin_Gram-ve
IPR013793 Porin_Gram-ve_CS
PfamiView protein in Pfam
PF00267 Porin_1, 1 hit
PRINTSiPR00183 ECOLIPORIN
PR00182 ECOLNEIPORIN
PROSITEiView protein in PROSITE
PS00576 GRAM_NEG_PORIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02931-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MMKRNILAVI VPALLVAGTA NAAEIYNKDG NKVDLYGKAV GLHYFSKGNG
60 70 80 90 100
ENSYGGNGDM TYARLGFKGE TQINSDLTGY GQWEYNFQGN NSEGADAQTG
110 120 130 140 150
NKTRLAFAGL KYADVGSFDY GRNYGVVYDA LGYTDMLPEF GGDTAYSDDF
160 170 180 190 200
FVGRVGGVAT YRNSNFFGLV DGLNFAVQYL GKNERDTARR SNGDGVGGSI
210 220 230 240 250
SYEYEGFGIV GAYGAADRTN LQEAQPLGNG KKAEQWATGL KYDANNIYLA
260 270 280 290 300
ANYGETRNAT PITNKFTNTS GFANKTQDVL LVAQYQFDFG LRPSIAYTKS
310 320 330 340 350
KAKDVEGIGD VDLVNYFEVG ATYYFNKNMS TYVDYIINQI DSDNKLGVGS
360
DDTVAVGIVY QF
Length:362
Mass (Da):39,333
Last modified:July 21, 1986 - v1
Checksum:i3F0974D96DB65464
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti88Q → E AA sequence (PubMed:7049161).Curated1
Sequence conflicti139E → G AA sequence (PubMed:7049161).Curated1
Sequence conflicti284Q → L AA sequence (PubMed:7049161).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01655 Genomic DNA Translation: AAA24244.1
U00096 Genomic DNA Translation: AAC74015.1
AP009048 Genomic DNA Translation: BAA35675.1
PIRiA93449 MMECF
RefSeqiNP_415449.1, NC_000913.3
WP_000977920.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74015; AAC74015; b0929
BAA35675; BAA35675; BAA35675
GeneIDi945554
KEGGiecj:JW0912
eco:b0929
PATRICifig|1411691.4.peg.1347

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01655 Genomic DNA Translation: AAA24244.1
U00096 Genomic DNA Translation: AAC74015.1
AP009048 Genomic DNA Translation: BAA35675.1
PIRiA93449 MMECF
RefSeqiNP_415449.1, NC_000913.3
WP_000977920.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BT9X-ray3.00A23-362[»]
1GFMX-ray3.50A23-362[»]
1GFNX-ray3.10A23-362[»]
1GFOX-ray3.30A23-362[»]
1GFPX-ray2.70A23-362[»]
1GFQX-ray2.80A23-362[»]
1HXTX-ray2.40A23-362[»]
1HXUX-ray3.00A23-362[»]
1HXXX-ray2.20A23-362[»]
1MPFX-ray3.00A23-362[»]
1OPFX-ray3.20A/B/C/D/E/F23-362[»]
2OMFX-ray2.40A23-362[»]
2ZFGX-ray1.59A23-362[»]
2ZLDX-ray3.00A/B23-362[»]
3FYXX-ray3.40A23-362[»]
3HW9X-ray2.61A/B1-362[»]
3HWBX-ray3.00A/B1-362[»]
3K19X-ray3.79A/B/C/D/E/F/G/H/I/J/K/L23-362[»]
3K1BX-ray4.39A/B/C/D23-362[»]
3O0EX-ray3.01A/B/C/D/E/F23-362[»]
3POQX-ray1.90A23-362[»]
3POUX-ray2.80A23-362[»]
3POXX-ray2.00A/B/C/D/E/F23-362[»]
4D5UX-ray3.50A/B/C/D/E/F23-362[»]
4GCPX-ray1.98A/B23-362[»]
4GCQX-ray2.20A/B23-362[»]
4GCSX-ray1.87A/B23-362[»]
4JFBX-ray3.80A/B/C/D/E/F23-362[»]
4LSEX-ray2.10A/B/C23-362[»]
4LSFX-ray1.90A/B23-362[»]
4LSHX-ray2.20A/B23-362[»]
4LSIX-ray2.09A/B/C23-362[»]
5NUOX-ray3.20A/C/E23-362[»]
5NUQX-ray3.20A/B/C/D/E/F23-362[»]
5NURX-ray3.29A/C/E23-362[»]
ProteinModelPortaliP02931
SMRiP02931
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260018, 306 interactors
DIPiDIP-10398N
IntActiP02931, 9 interactors
MINTiP02931
STRINGi316385.ECDH10B_0999

Chemistry databases

DrugBankiDB04233 (Hydroxyethyloxy)Tri(Ethyloxy)Octane

Protein family/group databases

TCDBi1.B.1.1.1 the general bacterial porin (gbp) family

2D gel databases

SWISS-2DPAGEiP02931

Proteomic databases

EPDiP02931
PaxDbiP02931
PRIDEiP02931

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74015; AAC74015; b0929
BAA35675; BAA35675; BAA35675
GeneIDi945554
KEGGiecj:JW0912
eco:b0929
PATRICifig|1411691.4.peg.1347

Organism-specific databases

EchoBASEiEB0665
EcoGeneiEG10671 ompF

Phylogenomic databases

eggNOGiENOG4105FCK Bacteria
COG3203 LUCA
HOGENOMiHOG000272406
InParanoidiP02931
KOiK09476
OMAiDAIGWTD
PhylomeDBiP02931

Enzyme and pathway databases

BioCyciEcoCyc:EG10671-MONOMER
MetaCyc:EG10671-MONOMER

Miscellaneous databases

EvolutionaryTraceiP02931
PROiPR:P02931

Family and domain databases

CDDicd00342 gram_neg_porins, 1 hit
Gene3Di2.40.160.10, 1 hit
InterProiView protein in InterPro
IPR033900 Gram_neg_porin_domain
IPR023614 Porin_dom_sf
IPR001897 Porin_gammaproteobac
IPR001702 Porin_Gram-ve
IPR013793 Porin_Gram-ve_CS
PfamiView protein in Pfam
PF00267 Porin_1, 1 hit
PRINTSiPR00183 ECOLIPORIN
PR00182 ECOLNEIPORIN
PROSITEiView protein in PROSITE
PS00576 GRAM_NEG_PORIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiOMPF_ECOLI
AccessioniPrimary (citable) accession number: P02931
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: October 10, 2018
This is version 186 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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