Outer membrane protein TolC

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functionⁱ

<p>This subsection of the ‘Function’ section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationⁱ

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functionⁱ

• efflux transmembrane transporter activity Source: EcoCyc <p>Inferred from Mutant Phenotype</p> <p>Describes annotations that are concluded from looking at variations or changes in a gene product such as mutations or abnormal levels and includes techniques such as knockouts, overexpression, anti-sense experiments and use of specific protein inhibitors.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#imp">GO evidence code guide</a></p> Inferred from mutant phenotypeⁱ
  • "TolC, an Escherichia coli outer membrane protein required for hemolysin secretion."
    Wandersman C., Delepelaire P.
    Proc. Natl. Acad. Sci. U.S.A. 87:4776-4780(1990) [PubMed] [Europe PMC] [Abstract]
• identical protein binding Source: IntAct <p>Inferred from Physical Interaction</p> <p>Covers physical interactions between the gene product of interest and another molecule (or ion, or complex).</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#ipi">GO evidence code guide</a></p> Inferred from physical interactionⁱ
  • Ref.19

    "Crystal structure of the bacterial membrane protein TolC central to multidrug efflux and protein export."
    Koronakis V., Sharff A., Koronakis E., Luisi B., Hughes C.
    Nature 405:914-919(2000) [PubMed] [Europe PMC] [Abstract]

    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 23-450, DOMAIN.
  • Ref.12

    "Protein complexes of the Escherichia coli cell envelope."
    Stenberg F., Chovanec P., Maslen S.L., Robinson C.V., Ilag L., von Heijne G., Daley D.O.
    J. Biol. Chem. 280:34409-34419(2005) [PubMed] [Europe PMC] [Abstract]

    Cited for: SUBUNIT, SUBCELLULAR LOCATION.
  • Ref.22

    "Structures of sequential open states in a symmetrical opening transition of the TolC exit duct."
    Pei X.Y., Hinchliffe P., Symmons M.F., Koronakis E., Benz R., Hughes C., Koronakis V.
    Proc. Natl. Acad. Sci. U.S.A. 108:2112-2117(2011) [PubMed] [Europe PMC] [Abstract]

    Cited for: X-RAY CRYSTALLOGRAPHY (2.85 ANGSTROMS) OF 23-450 OF MUTANTS.
  • "Structure-function of cyanobacterial outer-membrane protein, Slr1270: homolog of Escherichia coli drug export/colicin import protein, TolC."
    Agarwal R., Zakharov S., Hasan S.S., Ryan C.M., Whitelegge J.P., Cramer W.A.
    FEBS Lett. 588:3793-3801(2014) [PubMed] [Europe PMC] [Abstract]
• ion channel activity Source: CAFA <p>Inferred from Direct Assay</p> <p>Used to indicate a direct assay for the function, process or component indicated by the GO term.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#ida">GO evidence code guide</a></p> Inferred from direct assayⁱ
  • "Colicin occlusion of OmpF and TolC channels: outer membrane translocons for colicin import."
    Zakharov S.D., Eroukova V.Y., Rokitskaya T.I., Zhalnina M.V., Sharma O., Loll P.J., Zgurskaya H.I., Antonenko Y.N., Cramer W.A.
    Biophys. J. 87:3901-3911(2004) [PubMed] [Europe PMC] [Abstract]
• porin activity Source: EcoCycInferred from direct assayⁱ
  • "TolC of Escherichia coli functions as an outer membrane channel."
    Benz R., Maier E., Gentschev I.
    Zentralbl. Bakteriol. 278:187-196(1993) [PubMed] [Europe PMC] [Abstract]
• protein N-terminus binding Source: CAFAInferred from physical interactionⁱ
  • "Colicin occlusion of OmpF and TolC channels: outer membrane translocons for colicin import."
    Zakharov S.D., Eroukova V.Y., Rokitskaya T.I., Zhalnina M.V., Sharma O., Loll P.J., Zgurskaya H.I., Antonenko Y.N., Cramer W.A.
    Biophys. J. 87:3901-3911(2004) [PubMed] [Europe PMC] [Abstract]

GO - Biological processⁱ

• drug export Source: EcoCycInferred from direct assayⁱ
  • "Structure of the MacAB-TolC ABC-type tripartite multidrug efflux pump."
    Fitzpatrick A.W.P., Llabres S., Neuberger A., Blaza J.N., Bai X.C., Okada U., Murakami S., van Veen H.W., Zachariae U., Scheres S.H.W., Luisi B.F., Du D.
    Nat Microbiol 2:17070-17070(2017) [PubMed] [Europe PMC] [Abstract]
• enterobactin transport Source: EcoliWiki <p>Inferred from Genetic Interaction</p> <p>Used to describe “traditional” genetic interactions such as suppressors and synthetic lethals as well as other techniques such as functional complementation, rescue experiments, or inferences about a gene drawn from the phenotype of a mutation in a different gene.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#igi">GO evidence code guide</a></p> Inferred from genetic interactionⁱ
  • "TolC is involved in enterobactin efflux across the outer membrane of Escherichia coli."
    Bleuel C., Grosse C., Taudte N., Scherer J., Wesenberg D., Krauss G.J., Nies D.H., Grass G.
    J. Bacteriol. 187:6701-6707(2005) [PubMed] [Europe PMC] [Abstract]
• ion transmembrane transport Source: CAFAInferred from direct assayⁱ
  • "Colicin occlusion of OmpF and TolC channels: outer membrane translocons for colicin import."
    Zakharov S.D., Eroukova V.Y., Rokitskaya T.I., Zhalnina M.V., Sharma O., Loll P.J., Zgurskaya H.I., Antonenko Y.N., Cramer W.A.
    Biophys. J. 87:3901-3911(2004) [PubMed] [Europe PMC] [Abstract]
• response to antibiotic Source: UniProtKB-KW
• response to organic cyclic compound Source: EcoliWikiInferred from mutant phenotypeⁱ
  • "Involvement of outer membrane protein TolC, a possible member of the mar-sox regulon, in maintenance and improvement of organic solvent tolerance of Escherichia coli K-12."
    Aono R., Tsukagoshi N., Yamamoto M.
    J. Bacteriol. 180:938-944(1998) [PubMed] [Europe PMC] [Abstract]

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsⁱ

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyⁱ

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationⁱ

Topology

GO - Cellular componentⁱ

• cell outer membrane Source: EcoCycInferred from direct assayⁱ
  • "Proteomic analysis of the Escherichia coli outer membrane."
    Molloy M.P., Herbert B.R., Slade M.B., Rabilloud T., Nouwens A.S., Williams K.L., Gooley A.A.
    Eur. J. Biochem. 267:2871-2881(2000) [PubMed] [Europe PMC] [Abstract]
  • "Localization, annotation, and comparison of the Escherichia coli K-12 proteome under two states of growth."
    Lopez-Campistrous A., Semchuk P., Burke L., Palmer-Stone T., Brokx S.J., Broderick G., Bottorff D., Bolch S., Weiner J.H., Ellison M.J.
    Mol. Cell. Proteomics 4:1205-1209(2005) [PubMed] [Europe PMC] [Abstract]
  • "Comparison of SDS- and methanol-assisted protein solubilization and digestion methods for Escherichia coli membrane proteome analysis by 2-D LC-MS/MS."
    Zhang N., Chen R., Young N., Wishart D., Winter P., Weiner J.H., Li L.
    Proteomics 7:484-493(2007) [PubMed] [Europe PMC] [Abstract]
  • "Comparative analysis of envelope proteomes in Escherichia coli B and K-12 strains."
    Han M.J., Lee S.Y., Hong S.H.
    J. Microbiol. Biotechnol. 22:470-478(2012) [PubMed] [Europe PMC] [Abstract]
• efflux pump complex Source: EcoCycInferred from physical interactionⁱ
  • "AcrA, AcrB, and TolC of Escherichia coli Form a Stable Intermembrane Multidrug Efflux Complex."
    Tikhonova E.B., Zgurskaya H.I.
    J. Biol. Chem. 279:32116-32124(2004) [PubMed] [Europe PMC] [Abstract]
• integral component of membrane Source: CAFAInferred from direct assayⁱ
  • "Colicin occlusion of OmpF and TolC channels: outer membrane translocons for colicin import."
    Zakharov S.D., Eroukova V.Y., Rokitskaya T.I., Zhalnina M.V., Sharma O., Loll P.J., Zgurskaya H.I., Antonenko Y.N., Cramer W.A.
    Biophys. J. 87:3901-3911(2004) [PubMed] [Europe PMC] [Abstract]
• MacAB-TolC complex Source: EcoCycInferred from direct assayⁱ
  • "Structure of the MacAB-TolC ABC-type tripartite multidrug efflux pump."
    Fitzpatrick A.W.P., Llabres S., Neuberger A., Blaza J.N., Bai X.C., Okada U., Murakami S., van Veen H.W., Zachariae U., Scheres S.H.W., Luisi B.F., Du D.
    Nat Microbiol 2:17070-17070(2017) [PubMed] [Europe PMC] [Abstract]

Keywords - Cellular componentⁱ

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechⁱ

<p>This subsection of the ‘Pathology and Biotech’ section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypeⁱ

Mutagenesis

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingⁱ

Molecule processing

Proteomic databases

2D gel databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactionⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structureⁱ

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsⁱ

GO - Molecular functionⁱ

• identical protein binding Source: IntActInferred from physical interactionⁱ
  • Ref.19

    "Crystal structure of the bacterial membrane protein TolC central to multidrug efflux and protein export."
    Koronakis V., Sharff A., Koronakis E., Luisi B., Hughes C.
    Nature 405:914-919(2000) [PubMed] [Europe PMC] [Abstract]

    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 23-450, DOMAIN.
  • Ref.12

    "Protein complexes of the Escherichia coli cell envelope."
    Stenberg F., Chovanec P., Maslen S.L., Robinson C.V., Ilag L., von Heijne G., Daley D.O.
    J. Biol. Chem. 280:34409-34419(2005) [PubMed] [Europe PMC] [Abstract]

    Cited for: SUBUNIT, SUBCELLULAR LOCATION.
  • Ref.22

    "Structures of sequential open states in a symmetrical opening transition of the TolC exit duct."
    Pei X.Y., Hinchliffe P., Symmons M.F., Koronakis E., Benz R., Hughes C., Koronakis V.
    Proc. Natl. Acad. Sci. U.S.A. 108:2112-2117(2011) [PubMed] [Europe PMC] [Abstract]

    Cited for: X-RAY CRYSTALLOGRAPHY (2.85 ANGSTROMS) OF 23-450 OF MUTANTS.
  • "Structure-function of cyanobacterial outer-membrane protein, Slr1270: homolog of Escherichia coli drug export/colicin import protein, TolC."
    Agarwal R., Zakharov S., Hasan S.S., Ryan C.M., Whitelegge J.P., Cramer W.A.
    FEBS Lett. 588:3793-3801(2014) [PubMed] [Europe PMC] [Abstract]
• protein N-terminus binding Source: CAFAInferred from physical interactionⁱ
  • "Colicin occlusion of OmpF and TolC channels: outer membrane translocons for colicin import."
    Zakharov S.D., Eroukova V.Y., Rokitskaya T.I., Zhalnina M.V., Sharma O., Loll P.J., Zgurskaya H.I., Antonenko Y.N., Cramer W.A.
    Biophys. J. 87:3901-3911(2004) [PubMed] [Europe PMC] [Abstract]

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structureⁱ

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsⁱ

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides general information on the biological role of a domain. The term ‘domain’ is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domainⁱ

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesⁱ

Keywords - Domainⁱ

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequenceⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusⁱ: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingⁱ: The displayed sequence is further processed into a mature form.

        10         20         30         40         50
MKKLLPILIG LSLSGFSSLS QAENLMQVYQ QARLSNPELR KSAADRDAAF 
        60         70         80         90        100
EKINEARSPL LPQLGLGADY TYSNGYRDAN GINSNATSAS LQLTQSIFDM 
       110        120        130        140        150
SKWRALTLQE KAAGIQDVTY QTDQQTLILN TATAYFNVLN AIDVLSYTQA 
       160        170        180        190        200
QKEAIYRQLD QTTQRFNVGL VAITDVQNAR AQYDTVLANE VTARNNLDNA 
       210        220        230        240        250
VEQLRQITGN YYPELAALNV ENFKTDKPQP VNALLKEAEK RNLSLLQARL 
       260        270        280        290        300
SQDLAREQIR QAQDGHLPTL DLTASTGISD TSYSGSKTRG AAGTQYDDSN 
       310        320        330        340        350
MGQNKVGLSF SLPIYQGGMV NSQVKQAQYN FVGASEQLES AHRSVVQTVR 
       360        370        380        390        400
SSFNNINASI SSINAYKQAV VSAQSSLDAM EAGYSVGTRT IVDVLDATTT 
       410        420        430        440        450
LYNAKQELAN ARYNYLINQL NIKSALGTLN EQDLLALNNA LSKPVSTNPE 
       460        470        480        490 
NVAPQTPEQN AIADGYAPDS PAPVVQQTSA RTTTSNGHNP FRN        

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautionⁱ

Experimental Info

Sequence databases

Genome annotation databases

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsⁱ

• 100% Identity
• 90% Identity
• 50% Identity

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesⁱ

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

Protein family/group databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationⁱ

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousⁱ

Keywords - Technical termⁱ

Documents

1. PDB cross-references
   Index of Protein Data Bank (PDB) cross-references
2. SIMILARITY comments
   Index of protein domains and families
3. Escherichia coli
   Escherichia coli (strain K12): entries and cross-references to EcoGene