UniProtKB - P02930 (TOLC_ECOLI)
Protein
Outer membrane protein TolC
Gene
tolC
Organism
Escherichia coli (strain K12)
Status
Functioni
Outer membrane channel, which is required for the function of several efflux systems such as AcrAB-TolC, AcrEF-TolC, EmrAB-TolC and MacAB-TolC. These systems are involved in export of antibiotics and other toxic compounds from the cell. TolC is also involved in import of colicin E1 into the cells.5 Publications
Activity regulationi
In vitro, inhibited by hexaamminecobalt3+.1 Publication
GO - Molecular functioni
- bile acid transmembrane transporter activity Source: EcoCyc
- efflux transmembrane transporter activity Source: EcoCyc
- identical protein binding Source: IntAct
- ion channel activity Source: CAFA
- porin activity Source: EcoCyc
- protein N-terminus binding Source: CAFA
GO - Biological processi
- bile acid and bile salt transport Source: EcoCyc
- enterobactin transport Source: EcoliWiki
- ion transmembrane transport Source: CAFA
- response to antibiotic Source: EcoCyc
- response to drug Source: EcoCyc
- response to organic cyclic compound Source: EcoliWiki
- response to toxic substance Source: EcoCyc
Keywordsi
Biological process | Antibiotic resistance, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11009-MONOMER MetaCyc:EG11009-MONOMER |
Protein family/group databases
TCDBi | 1.B.17.1.1, the outer membrane factor (omf) family |
Names & Taxonomyi
Protein namesi | Recommended name: Outer membrane protein TolCAlternative name(s): Multidrug efflux pump subunit TolC Outer membrane factor TolC |
Gene namesi | Name:tolC Synonyms:colE1-i, mtcB, mukA, refI, toc, weeA Ordered Locus Names:b3035, JW5503 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Other locations
- Cell outer membrane 5 Publications; Multi-pass membrane protein 5 Publications
Other locations
- cell outer membrane Source: EcoCyc
- efflux pump complex Source: EcoCyc
- integral component of cell outer membrane Source: EcoCyc
- integral component of membrane Source: CAFA
- MacAB-TolC complex Source: EcoCyc
- outer membrane-bounded periplasmic space Source: EcoCyc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 23 – 62 | PeriplasmicAdd BLAST | 40 | |
Transmembranei | 63 – 74 | Beta stranded; Name=S1Add BLAST | 12 | |
Topological domaini | 75 – 82 | Extracellular | 8 | |
Transmembranei | 83 – 96 | Beta stranded; Name=S2Add BLAST | 14 | |
Topological domaini | 97 – 268 | PeriplasmicAdd BLAST | 172 | |
Transmembranei | 269 – 279 | Beta stranded; Name=S4Add BLAST | 11 | |
Topological domaini | 280 – 300 | ExtracellularAdd BLAST | 21 | |
Transmembranei | 301 – 311 | Beta stranded; Name=S5Add BLAST | 11 | |
Topological domaini | 312 – 493 | PeriplasmicAdd BLAST | 182 |
Keywords - Cellular componenti
Cell outer membrane, MembranePathology & Biotechi
Disruption phenotypei
Cannot grow on efflux substrates novobiocin or fusidic acid.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 384 | Y → F: Partial channel opening. Increases sensitivity to vancomycin, by allowing its passive diffusion across the outer membrane; when associated with E-389. 1 Publication | 1 | |
Mutagenesisi | 389 | R → E: Partial channel opening. Increases sensitivity to vancomycin, by allowing its passive diffusion across the outer membrane; when associated with F-382. 1 Publication | 1 | |
Mutagenesisi | 393 | D → A: Decreases inhibition by hexaamminecobalt(3+). 1 Publication | 1 | |
Mutagenesisi | 396 | D → A: Decreases inhibition by hexaamminecobalt(3+). 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL3309023 |
DrugBanki | DB03350, Cobalt hexammine ion |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | 3 PublicationsAdd BLAST | 22 | |
ChainiPRO_0000013352 | 23 – 493 | Outer membrane protein TolCAdd BLAST | 471 |
Proteomic databases
jPOSTi | P02930 |
PaxDbi | P02930 |
PRIDEi | P02930 |
2D gel databases
SWISS-2DPAGEi | P02930 |
Interactioni
Subunit structurei
Homotrimer. Part of tripartite efflux systems, which are composed of an inner membrane transporter, a periplasmic membrane fusion protein, and an outer membrane component, TolC. The complexes form a large protein conduit and can translocate molecules across both the inner and outer membranes. TolC interacts with the membrane fusion proteins AcrA, EmrA and MacA.
8 PublicationsBinary interactionsi
Hide detailsP02930
With | #Exp. | IntAct |
---|---|---|
acrA [P0AE06] | 2 | EBI-875614,EBI-875601 |
macA [P75830] | 3 | EBI-875614,EBI-551961 |
itself | 6 | EBI-875614,EBI-875614 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- protein N-terminus binding Source: CAFA
Protein-protein interaction databases
BioGRIDi | 4263248, 406 interactors 851838, 1 interactor |
ComplexPortali | CPX-2107, MacAB-TolC ABC transporter complex CPX-2119, MdtABC-TolC multidrug efflux transport complex CPX-4263, AcrAB-TolC multidrug efflux transport complex CPX-4264, AcrAD-TolC multidrug efflux transport complex CPX-4265, AcrEF-TolC multidrug efflux transport complex CPX-4268, EmrAB-TolC multidrug efflux transport system CPX-4273, EmrKY-TolC multidrug efflux transport system |
DIPi | DIP-11007N |
IntActi | P02930, 7 interactors |
MINTi | P02930 |
STRINGi | 511145.b3035 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P02930 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P02930 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 23 – 230 | 1Add BLAST | 208 | |
Repeati | 231 – 446 | 2Add BLAST | 216 |
Domaini
Forms a continuous, solvent-accessible conduit: a 'channel-tunnel' over 140 Angstroms long that spans both the outer membrane and periplasmic space. The periplasmic or proximal end of the tunnel is sealed by sets of coiled helices.1 Publication
Sequence similaritiesi
Keywords - Domaini
Repeat, Signal, Transmembrane, Transmembrane beta strandPhylogenomic databases
eggNOGi | COG1538, Bacteria |
HOGENOMi | CLU_012817_0_2_6 |
InParanoidi | P02930 |
PhylomeDBi | P02930 |
Family and domain databases
InterProi | View protein in InterPro IPR003423, OMP_efflux IPR010130, T1SS_OMP_TolC |
Pfami | View protein in Pfam PF02321, OEP, 2 hits |
TIGRFAMsi | TIGR01844, type_I_sec_TolC, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P02930-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKKLLPILIG LSLSGFSSLS QAENLMQVYQ QARLSNPELR KSAADRDAAF
60 70 80 90 100
EKINEARSPL LPQLGLGADY TYSNGYRDAN GINSNATSAS LQLTQSIFDM
110 120 130 140 150
SKWRALTLQE KAAGIQDVTY QTDQQTLILN TATAYFNVLN AIDVLSYTQA
160 170 180 190 200
QKEAIYRQLD QTTQRFNVGL VAITDVQNAR AQYDTVLANE VTARNNLDNA
210 220 230 240 250
VEQLRQITGN YYPELAALNV ENFKTDKPQP VNALLKEAEK RNLSLLQARL
260 270 280 290 300
SQDLAREQIR QAQDGHLPTL DLTASTGISD TSYSGSKTRG AAGTQYDDSN
310 320 330 340 350
MGQNKVGLSF SLPIYQGGMV NSQVKQAQYN FVGASEQLES AHRSVVQTVR
360 370 380 390 400
SSFNNINASI SSINAYKQAV VSAQSSLDAM EAGYSVGTRT IVDVLDATTT
410 420 430 440 450
LYNAKQELAN ARYNYLINQL NIKSALGTLN EQDLLALNNA LSKPVSTNPE
460 470 480 490
NVAPQTPEQN AIADGYAPDS PAPVVQQTSA RTTTSNGHNP FRN
Sequence cautioni
The sequence AAA69203 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence CAA24751 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence CAA37982 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 178 | N → K in CAA24914 (PubMed:6312426).Curated | 1 | |
Sequence conflicti | 191 | V → L in CAA37982 (PubMed:2216730).Curated | 1 | |
Sequence conflicti | 203 – 204 | QL → HV in CAA24914 (PubMed:6312426).Curated | 2 | |
Sequence conflicti | 214 – 215 | EL → GT in CAA24914 (PubMed:6312426).Curated | 2 | |
Sequence conflicti | 258 – 270 | QIRQA…HLPTL → KFARRRMVTYRLW in CAA24914 (PubMed:6312426).CuratedAdd BLAST | 13 | |
Sequence conflicti | 278 – 295 | ISDTS…AAGTQ → FLTPLIAVRKPCAAVP in CAA24914 (PubMed:6312426).CuratedAdd BLAST | 18 | |
Sequence conflicti | 325 | K → T in CAA24914 (PubMed:6312426).Curated | 1 | |
Sequence conflicti | 335 – 354 | SEQLE…RSSFN → ASTWKVPIVASCQRAFCFS in CAA24914 (PubMed:6312426).CuratedAdd BLAST | 20 | |
Sequence conflicti | 365 – 370 | AYKQAV → RYTQAA in CAA24914 (PubMed:6312426).Curated | 6 | |
Sequence conflicti | 400 – 411 | TLYNA…ELANA → SCTAQARAGNP in CAA24914 (PubMed:6312426).CuratedAdd BLAST | 12 | |
Sequence conflicti | 445 | V → I in CAA24914 (PubMed:6312426).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X00016 Genomic DNA Translation: CAA24914.1 X54049 Genomic DNA Translation: CAA37982.1 Different initiation. U28377 Genomic DNA Translation: AAA69203.1 Different initiation. U00096 Genomic DNA Translation: AAC76071.2 AP009048 Genomic DNA Translation: BAE77091.1 V01505 Genomic DNA Translation: CAA24751.1 Different initiation. |
PIRi | A65091, MMECTC |
RefSeqi | NP_417507.2, NC_000913.3 WP_000735278.1, NZ_STEB01000001.1 |
Genome annotation databases
EnsemblBacteriai | AAC76071; AAC76071; b3035 BAE77091; BAE77091; BAE77091 |
GeneIDi | 57729143 947521 |
KEGGi | ecj:JW5503 eco:b3035 |
PATRICi | fig|1411691.4.peg.3696 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X00016 Genomic DNA Translation: CAA24914.1 X54049 Genomic DNA Translation: CAA37982.1 Different initiation. U28377 Genomic DNA Translation: AAA69203.1 Different initiation. U00096 Genomic DNA Translation: AAC76071.2 AP009048 Genomic DNA Translation: BAE77091.1 V01505 Genomic DNA Translation: CAA24751.1 Different initiation. |
PIRi | A65091, MMECTC |
RefSeqi | NP_417507.2, NC_000913.3 WP_000735278.1, NZ_STEB01000001.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1EK9 | X-ray | 2.10 | A/B/C | 23-450 | [»] | |
1TQQ | X-ray | 2.75 | A/B/C | 23-493 | [»] | |
2VDD | X-ray | 3.30 | A/B/C | 1-450 | [»] | |
2VDE | X-ray | 3.20 | A/B/C | 1-450 | [»] | |
2WMZ | X-ray | 2.90 | A/B/C | 23-450 | [»] | |
2XMN | X-ray | 2.85 | A/B/C | 23-450 | [»] | |
5NG5 | electron microscopy | 6.50 | C/F/I | 1-493 | [»] | |
5NIK | electron microscopy | 3.30 | A/B/C | 23-493 | [»] | |
5NIL | electron microscopy | 5.30 | A/B/C | 23-493 | [»] | |
5O66 | electron microscopy | 5.90 | A/B/C | 1-493 | [»] | |
5V5S | electron microscopy | 6.50 | A/B/C | 23-464 | [»] | |
SMRi | P02930 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263248, 406 interactors 851838, 1 interactor |
ComplexPortali | CPX-2107, MacAB-TolC ABC transporter complex CPX-2119, MdtABC-TolC multidrug efflux transport complex CPX-4263, AcrAB-TolC multidrug efflux transport complex CPX-4264, AcrAD-TolC multidrug efflux transport complex CPX-4265, AcrEF-TolC multidrug efflux transport complex CPX-4268, EmrAB-TolC multidrug efflux transport system CPX-4273, EmrKY-TolC multidrug efflux transport system |
DIPi | DIP-11007N |
IntActi | P02930, 7 interactors |
MINTi | P02930 |
STRINGi | 511145.b3035 |
Chemistry databases
ChEMBLi | CHEMBL3309023 |
DrugBanki | DB03350, Cobalt hexammine ion |
Protein family/group databases
TCDBi | 1.B.17.1.1, the outer membrane factor (omf) family |
2D gel databases
SWISS-2DPAGEi | P02930 |
Proteomic databases
jPOSTi | P02930 |
PaxDbi | P02930 |
PRIDEi | P02930 |
Genome annotation databases
EnsemblBacteriai | AAC76071; AAC76071; b3035 BAE77091; BAE77091; BAE77091 |
GeneIDi | 57729143 947521 |
KEGGi | ecj:JW5503 eco:b3035 |
PATRICi | fig|1411691.4.peg.3696 |
Organism-specific databases
EchoBASEi | EB1002 |
Phylogenomic databases
eggNOGi | COG1538, Bacteria |
HOGENOMi | CLU_012817_0_2_6 |
InParanoidi | P02930 |
PhylomeDBi | P02930 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11009-MONOMER MetaCyc:EG11009-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P02930 |
PROi | PR:P02930 |
Family and domain databases
InterProi | View protein in InterPro IPR003423, OMP_efflux IPR010130, T1SS_OMP_TolC |
Pfami | View protein in Pfam PF02321, OEP, 2 hits |
TIGRFAMsi | TIGR01844, type_I_sec_TolC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TOLC_ECOLI | |
Accessioni | P02930Primary (citable) accession number: P02930 Secondary accession number(s): Q2M9G5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | June 27, 2006 | |
Last modified: | April 7, 2021 | |
This is version 194 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families