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Protein

Outer membrane protein TolC

Gene

tolC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Outer membrane channel, which is required for the function of several efflux systems such as AcrAB-TolC, AcrEF-TolC, EmrAB-TolC and MacAB-TolC. These systems are involved in export of antibiotics and other toxic compounds from the cell. TolC is also involved in import of colicin E1 into the cells.5 Publications

Activity regulationi

In vitro, inhibited by hexaamminecobalt3+.1 Publication

GO - Molecular functioni

  • efflux transmembrane transporter activity Source: EcoCyc
  • identical protein binding Source: IntAct
  • ion channel activity Source: CAFA
  • porin activity Source: EcoCyc
  • protein N-terminus binding Source: CAFA

GO - Biological processi

  • drug export Source: EcoCyc
  • enterobactin transport Source: EcoliWiki
  • ion transmembrane transport Source: CAFA
  • response to antibiotic Source: UniProtKB-KW
  • response to organic cyclic compound Source: EcoliWiki

Keywordsi

Biological processAntibiotic resistance, Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG11009-MONOMER
MetaCyc:EG11009-MONOMER

Protein family/group databases

TCDBi1.B.17.1.1 the outer membrane factor (omf) family

Names & Taxonomyi

Protein namesi
Recommended name:
Outer membrane protein TolC
Alternative name(s):
Multidrug efflux pump subunit TolC
Outer membrane factor TolC
Gene namesi
Name:tolC
Synonyms:colE1-i, mtcB, mukA, refI, toc, weeA
Ordered Locus Names:b3035, JW5503
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11009 tolC

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 62PeriplasmicAdd BLAST40
Transmembranei63 – 74Beta stranded; Name=S1Add BLAST12
Topological domaini75 – 82Extracellular8
Transmembranei83 – 96Beta stranded; Name=S2Add BLAST14
Topological domaini97 – 268PeriplasmicAdd BLAST172
Transmembranei269 – 279Beta stranded; Name=S4Add BLAST11
Topological domaini280 – 300ExtracellularAdd BLAST21
Transmembranei301 – 311Beta stranded; Name=S5Add BLAST11
Topological domaini312 – 493PeriplasmicAdd BLAST182

GO - Cellular componenti

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Cannot grow on efflux substrates novobiocin or fusidic acid.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi384Y → F: Partial channel opening. Increases sensitivity to vancomycin, by allowing its passive diffusion across the outer membrane; when associated with E-389. 1 Publication1
Mutagenesisi389R → E: Partial channel opening. Increases sensitivity to vancomycin, by allowing its passive diffusion across the outer membrane; when associated with F-382. 1 Publication1
Mutagenesisi393D → A: Decreases inhibition by hexaamminecobalt(3+). 1 Publication1
Mutagenesisi396D → A: Decreases inhibition by hexaamminecobalt(3+). 1 Publication1

Chemistry databases

ChEMBLiCHEMBL3309023
DrugBankiDB03350 Cobalt Hexammine Ion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 223 PublicationsAdd BLAST22
ChainiPRO_000001335223 – 493Outer membrane protein TolCAdd BLAST471

Proteomic databases

EPDiP02930
PaxDbiP02930
PRIDEiP02930

2D gel databases

SWISS-2DPAGEiP02930

Interactioni

Subunit structurei

Homotrimer. Part of tripartite efflux systems, which are composed of an inner membrane transporter, a periplasmic membrane fusion protein, and an outer membrane component, TolC. The complexes form a large protein conduit and can translocate molecules across both the inner and outer membranes. TolC interacts with the membrane fusion proteins AcrA, EmrA and MacA.8 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4263248, 406 interactors
ComplexPortaliCPX-2107 MacAB-TolC complex
DIPiDIP-11007N
IntActiP02930, 6 interactors
MINTiP02930
STRINGi316385.ECDH10B_3209

Structurei

Secondary structure

1493
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP02930
SMRiP02930
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02930

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati23 – 2301Add BLAST208
Repeati231 – 4462Add BLAST216

Domaini

Forms a continuous, solvent-accessible conduit: a 'channel-tunnel' over 140 Angstroms long that spans both the outer membrane and periplasmic space. The periplasmic or proximal end of the tunnel is sealed by sets of coiled helices.1 Publication

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4105DFG Bacteria
COG1538 LUCA
HOGENOMiHOG000283506
InParanoidiP02930
KOiK12340
OMAiYNAKQQL
PhylomeDBiP02930

Family and domain databases

InterProiView protein in InterPro
IPR003423 OMP_efflux
IPR010130 T1SS_OMP_TolC
PfamiView protein in Pfam
PF02321 OEP, 2 hits
TIGRFAMsiTIGR01844 type_I_sec_TolC, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02930-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKKLLPILIG LSLSGFSSLS QAENLMQVYQ QARLSNPELR KSAADRDAAF
60 70 80 90 100
EKINEARSPL LPQLGLGADY TYSNGYRDAN GINSNATSAS LQLTQSIFDM
110 120 130 140 150
SKWRALTLQE KAAGIQDVTY QTDQQTLILN TATAYFNVLN AIDVLSYTQA
160 170 180 190 200
QKEAIYRQLD QTTQRFNVGL VAITDVQNAR AQYDTVLANE VTARNNLDNA
210 220 230 240 250
VEQLRQITGN YYPELAALNV ENFKTDKPQP VNALLKEAEK RNLSLLQARL
260 270 280 290 300
SQDLAREQIR QAQDGHLPTL DLTASTGISD TSYSGSKTRG AAGTQYDDSN
310 320 330 340 350
MGQNKVGLSF SLPIYQGGMV NSQVKQAQYN FVGASEQLES AHRSVVQTVR
360 370 380 390 400
SSFNNINASI SSINAYKQAV VSAQSSLDAM EAGYSVGTRT IVDVLDATTT
410 420 430 440 450
LYNAKQELAN ARYNYLINQL NIKSALGTLN EQDLLALNNA LSKPVSTNPE
460 470 480 490
NVAPQTPEQN AIADGYAPDS PAPVVQQTSA RTTTSNGHNP FRN
Length:493
Mass (Da):53,741
Last modified:June 27, 2006 - v3
Checksum:i6F97B4C62A848FE1
GO

Sequence cautioni

The sequence AAA69203 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA24751 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA37982 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti178N → K in CAA24914 (PubMed:6312426).Curated1
Sequence conflicti191V → L in CAA37982 (PubMed:2216730).Curated1
Sequence conflicti203 – 204QL → HV in CAA24914 (PubMed:6312426).Curated2
Sequence conflicti214 – 215EL → GT in CAA24914 (PubMed:6312426).Curated2
Sequence conflicti258 – 270QIRQA…HLPTL → KFARRRMVTYRLW in CAA24914 (PubMed:6312426).CuratedAdd BLAST13
Sequence conflicti278 – 295ISDTS…AAGTQ → FLTPLIAVRKPCAAVP in CAA24914 (PubMed:6312426).CuratedAdd BLAST18
Sequence conflicti325K → T in CAA24914 (PubMed:6312426).Curated1
Sequence conflicti335 – 354SEQLE…RSSFN → ASTWKVPIVASCQRAFCFS in CAA24914 (PubMed:6312426).CuratedAdd BLAST20
Sequence conflicti365 – 370AYKQAV → RYTQAA in CAA24914 (PubMed:6312426).Curated6
Sequence conflicti400 – 411TLYNA…ELANA → SCTAQARAGNP in CAA24914 (PubMed:6312426).CuratedAdd BLAST12
Sequence conflicti445V → I in CAA24914 (PubMed:6312426).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00016 Genomic DNA Translation: CAA24914.1
X54049 Genomic DNA Translation: CAA37982.1 Different initiation.
U28377 Genomic DNA Translation: AAA69203.1 Different initiation.
U00096 Genomic DNA Translation: AAC76071.2
AP009048 Genomic DNA Translation: BAE77091.1
V01505 Genomic DNA Translation: CAA24751.1 Different initiation.
PIRiA65091 MMECTC
RefSeqiNP_417507.2, NC_000913.3
WP_000735278.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76071; AAC76071; b3035
BAE77091; BAE77091; BAE77091
GeneIDi947521
KEGGiecj:JW5503
eco:b3035
PATRICifig|1411691.4.peg.3696

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00016 Genomic DNA Translation: CAA24914.1
X54049 Genomic DNA Translation: CAA37982.1 Different initiation.
U28377 Genomic DNA Translation: AAA69203.1 Different initiation.
U00096 Genomic DNA Translation: AAC76071.2
AP009048 Genomic DNA Translation: BAE77091.1
V01505 Genomic DNA Translation: CAA24751.1 Different initiation.
PIRiA65091 MMECTC
RefSeqiNP_417507.2, NC_000913.3
WP_000735278.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EK9X-ray2.10A/B/C23-450[»]
1TQQX-ray2.75A/B/C23-493[»]
2VDDX-ray3.30A/B/C1-450[»]
2VDEX-ray3.20A/B/C1-450[»]
2WMZX-ray2.90A/B/C23-450[»]
2XMNX-ray2.85A/B/C23-450[»]
5NG5electron microscopy6.50C/F/I1-493[»]
5NIKelectron microscopy3.30A/B/C23-493[»]
5NILelectron microscopy5.30A/B/C23-493[»]
5O66electron microscopy5.90A/B/C1-493[»]
5V5Selectron microscopy6.50A/B/C23-464[»]
ProteinModelPortaliP02930
SMRiP02930
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263248, 406 interactors
ComplexPortaliCPX-2107 MacAB-TolC complex
DIPiDIP-11007N
IntActiP02930, 6 interactors
MINTiP02930
STRINGi316385.ECDH10B_3209

Chemistry databases

ChEMBLiCHEMBL3309023
DrugBankiDB03350 Cobalt Hexammine Ion

Protein family/group databases

TCDBi1.B.17.1.1 the outer membrane factor (omf) family

2D gel databases

SWISS-2DPAGEiP02930

Proteomic databases

EPDiP02930
PaxDbiP02930
PRIDEiP02930

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76071; AAC76071; b3035
BAE77091; BAE77091; BAE77091
GeneIDi947521
KEGGiecj:JW5503
eco:b3035
PATRICifig|1411691.4.peg.3696

Organism-specific databases

EchoBASEiEB1002
EcoGeneiEG11009 tolC

Phylogenomic databases

eggNOGiENOG4105DFG Bacteria
COG1538 LUCA
HOGENOMiHOG000283506
InParanoidiP02930
KOiK12340
OMAiYNAKQQL
PhylomeDBiP02930

Enzyme and pathway databases

BioCyciEcoCyc:EG11009-MONOMER
MetaCyc:EG11009-MONOMER

Miscellaneous databases

EvolutionaryTraceiP02930
PROiPR:P02930

Family and domain databases

InterProiView protein in InterPro
IPR003423 OMP_efflux
IPR010130 T1SS_OMP_TolC
PfamiView protein in Pfam
PF02321 OEP, 2 hits
TIGRFAMsiTIGR01844 type_I_sec_TolC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTOLC_ECOLI
AccessioniPrimary (citable) accession number: P02930
Secondary accession number(s): Q2M9G5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: June 27, 2006
Last modified: September 12, 2018
This is version 176 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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