UniProtKB - P02924 (ARAF_ECOLI)
Protein
L-arabinose-binding periplasmic protein
Gene
araF
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in the high-affinity L-arabinose membrane transport system. Binds with high affinity to arabinose, but can also bind D-galactose (approximately 2-fold reduction) and D-fucose (approximately 40-fold reduction).
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 87 | The binding site for the sugar molecule has not yet been established, but C-87 may be involved | 1 |
GO - Molecular functioni
- ATPase-coupled monosaccharide transmembrane transporter activity Source: EcoCyc
- carbohydrate binding Source: GO_Central
- monosaccharide binding Source: EcoCyc
GO - Biological processi
- L-arabinose transmembrane transport Source: InterPro
Keywordsi
Biological process | Sugar transport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:ARAF-MONOMER MetaCyc:ARAF-MONOMER |
Protein family/group databases
TCDBi | 3.A.1.2.2, the atp-binding cassette (abc) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: L-arabinose-binding periplasmic proteinShort name: ABP |
Gene namesi | Name:araF Ordered Locus Names:b1901, JW1889 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- outer membrane-bounded periplasmic space Source: EcoCyc
Keywords - Cellular componenti
PeriplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 277 | P → G: Improves binding to galactose. | 1 |
Chemistry databases
DrugBanki | DB03485, alpha-D-Fucopyranose DB03142, Alpha-L-Arabinose DB04062, beta-D-fucose DB03246, Beta-L-Arabinose |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 23 | 2 PublicationsAdd BLAST | 23 | |
ChainiPRO_0000031720 | 24 – 329 | L-arabinose-binding periplasmic proteinAdd BLAST | 306 |
Proteomic databases
jPOSTi | P02924 |
PaxDbi | P02924 |
PRIDEi | P02924 |
Expressioni
Inductioni
Induced by arabinose. Transcription is dependent on the transcription factor AraC, the cAMP receptor protein (CRP) and cAMP.1 Publication
Interactioni
Protein-protein interaction databases
BioGRIDi | 4261850, 21 interactors |
ComplexPortali | CPX-4314, Arabinose ABC transporter complex |
IntActi | P02924, 6 interactors |
STRINGi | 511145.b1901 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P02924 |
SMRi | P02924 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P02924 |
Family & Domainsi
Sequence similaritiesi
Belongs to the bacterial solute-binding protein 2 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG1879, Bacteria |
HOGENOMi | CLU_046821_1_0_6 |
PhylomeDBi | P02924 |
Family and domain databases
CDDi | cd01540, PBP1_arabinose_binding, 1 hit |
InterProi | View protein in InterPro IPR026266, AraF IPR001761, Peripla_BP/Lac1_sug-bd_dom IPR028082, Peripla_BP_I |
Pfami | View protein in Pfam PF00532, Peripla_BP_1, 1 hit |
PIRSFi | PIRSF002816, AraF, 1 hit |
SUPFAMi | SSF53822, SSF53822, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P02924-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MHKFTKALAA IGLAAVMSQS AMAENLKLGF LVKQPEEPWF QTEWKFADKA
60 70 80 90 100
GKDLGFEVIK IAVPDGEKTL NAIDSLAASG AKGFVICTPD PKLGSAIVAK
110 120 130 140 150
ARGYDMKVIA VDDQFVNAKG KPMDTVPLVM MAATKIGERQ GQELYKEMQK
160 170 180 190 200
RGWDVKESAV MAITANELDT ARRRTTGSMD ALKAAGFPEK QIYQVPTKSN
210 220 230 240 250
DIPGAFDAAN SMLVQHPEVK HWLIVGMNDS TVLGGVRATE GQGFKAADII
260 270 280 290 300
GIGINGVDAV SELSKAQATG FYGSLLPSPD VHGYKSSEML YNWVAKDVEP
310 320
PKFTEVTDVV LITRDNFKEE LEKKGLGGK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 194 | Q → E in CAA29476 (PubMed:2445996).Curated | 1 | |
Sequence conflicti | 281 | V → L in CAA29476 (PubMed:2445996).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X06091 Genomic DNA Translation: CAA29476.1 U00096 Genomic DNA Translation: AAC74971.1 AP009048 Genomic DNA Translation: BAA15721.1 K00420 Genomic DNA Translation: AAA23472.1 |
PIRi | E64953, JGECA |
RefSeqi | NP_416414.1, NC_000913.3 WP_000548675.1, NZ_STEB01000026.1 |
Genome annotation databases
EnsemblBacteriai | AAC74971; AAC74971; b1901 BAA15721; BAA15721; BAA15721 |
GeneIDi | 946409 |
KEGGi | ecj:JW1889 eco:b1901 |
PATRICi | fig|1411691.4.peg.349 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X06091 Genomic DNA Translation: CAA29476.1 U00096 Genomic DNA Translation: AAC74971.1 AP009048 Genomic DNA Translation: BAA15721.1 K00420 Genomic DNA Translation: AAA23472.1 |
PIRi | E64953, JGECA |
RefSeqi | NP_416414.1, NC_000913.3 WP_000548675.1, NZ_STEB01000026.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1ABE | X-ray | 1.70 | A | 24-329 | [»] | |
1ABF | X-ray | 1.90 | A | 24-329 | [»] | |
1APB | X-ray | 1.76 | A | 24-329 | [»] | |
1BAP | X-ray | 1.75 | A | 24-329 | [»] | |
2WRZ | X-ray | 2.20 | A/B | 24-329 | [»] | |
5ABP | X-ray | 1.80 | A | 24-329 | [»] | |
6ABP | X-ray | 1.67 | A | 24-329 | [»] | |
7ABP | X-ray | 1.67 | A | 24-329 | [»] | |
8ABP | X-ray | 1.49 | A | 24-329 | [»] | |
9ABP | X-ray | 1.97 | A | 24-329 | [»] | |
BMRBi | P02924 | |||||
SMRi | P02924 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261850, 21 interactors |
ComplexPortali | CPX-4314, Arabinose ABC transporter complex |
IntActi | P02924, 6 interactors |
STRINGi | 511145.b1901 |
Chemistry databases
DrugBanki | DB03485, alpha-D-Fucopyranose DB03142, Alpha-L-Arabinose DB04062, beta-D-fucose DB03246, Beta-L-Arabinose |
Protein family/group databases
TCDBi | 3.A.1.2.2, the atp-binding cassette (abc) superfamily |
Proteomic databases
jPOSTi | P02924 |
PaxDbi | P02924 |
PRIDEi | P02924 |
Genome annotation databases
EnsemblBacteriai | AAC74971; AAC74971; b1901 BAA15721; BAA15721; BAA15721 |
GeneIDi | 946409 |
KEGGi | ecj:JW1889 eco:b1901 |
PATRICi | fig|1411691.4.peg.349 |
Organism-specific databases
EchoBASEi | EB0055 |
Phylogenomic databases
eggNOGi | COG1879, Bacteria |
HOGENOMi | CLU_046821_1_0_6 |
PhylomeDBi | P02924 |
Enzyme and pathway databases
BioCyci | EcoCyc:ARAF-MONOMER MetaCyc:ARAF-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P02924 |
PROi | PR:P02924 |
Family and domain databases
CDDi | cd01540, PBP1_arabinose_binding, 1 hit |
InterProi | View protein in InterPro IPR026266, AraF IPR001761, Peripla_BP/Lac1_sug-bd_dom IPR028082, Peripla_BP_I |
Pfami | View protein in Pfam PF00532, Peripla_BP_1, 1 hit |
PIRSFi | PIRSF002816, AraF, 1 hit |
SUPFAMi | SSF53822, SSF53822, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ARAF_ECOLI | |
Accessioni | P02924Primary (citable) accession number: P02924 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | November 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 171 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families