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Protein

L-arabinose-binding periplasmic protein

Gene

araF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the high-affinity L-arabinose membrane transport system. Binds with high affinity to arabinose, but can also bind D-galactose (approximately 2-fold reduction) and D-fucose (approximately 40-fold reduction).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei87The binding site for the sugar molecule has not yet been established, but C-87 may be involved1

GO - Molecular functioni

  • monosaccharide binding Source: EcoCyc
  • monosaccharide-transporting ATPase activity Source: EcoCyc

GO - Biological processi

Keywordsi

Biological processSugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:ARAF-MONOMER
MetaCyc:ARAF-MONOMER

Protein family/group databases

TCDBi3.A.1.2.2 the atp-binding cassette (abc) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
L-arabinose-binding periplasmic protein
Short name:
ABP
Gene namesi
Name:araF
Ordered Locus Names:b1901, JW1889
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10057 araF

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi277P → G: Improves binding to galactose. 1

Chemistry databases

DrugBankiDB03485 Alpha-D-Fucose
DB03142 Alpha-L-Arabinose
DB04062 Beta-D-Fucose
DB03246 Beta-L-Arabinose

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 232 PublicationsAdd BLAST23
ChainiPRO_000003172024 – 329L-arabinose-binding periplasmic proteinAdd BLAST306

Proteomic databases

EPDiP02924
PaxDbiP02924
PRIDEiP02924

Interactioni

Protein-protein interaction databases

BioGridi4261850, 21 interactors
IntActiP02924, 5 interactors
STRINGi316385.ECDH10B_2041

Structurei

Secondary structure

1329
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP02924
SMRiP02924
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02924

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105DMK Bacteria
COG1879 LUCA
HOGENOMiHOG000220638
KOiK10537
PhylomeDBiP02924

Family and domain databases

CDDicd01540 PBP1_arabinose_binding, 1 hit
InterProiView protein in InterPro
IPR026266 AraF
IPR001761 Peripla_BP/Lac1_sug-bd_dom
IPR028082 Peripla_BP_I
PfamiView protein in Pfam
PF00532 Peripla_BP_1, 1 hit
PIRSFiPIRSF002816 AraF, 1 hit
SUPFAMiSSF53822 SSF53822, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02924-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MHKFTKALAA IGLAAVMSQS AMAENLKLGF LVKQPEEPWF QTEWKFADKA
60 70 80 90 100
GKDLGFEVIK IAVPDGEKTL NAIDSLAASG AKGFVICTPD PKLGSAIVAK
110 120 130 140 150
ARGYDMKVIA VDDQFVNAKG KPMDTVPLVM MAATKIGERQ GQELYKEMQK
160 170 180 190 200
RGWDVKESAV MAITANELDT ARRRTTGSMD ALKAAGFPEK QIYQVPTKSN
210 220 230 240 250
DIPGAFDAAN SMLVQHPEVK HWLIVGMNDS TVLGGVRATE GQGFKAADII
260 270 280 290 300
GIGINGVDAV SELSKAQATG FYGSLLPSPD VHGYKSSEML YNWVAKDVEP
310 320
PKFTEVTDVV LITRDNFKEE LEKKGLGGK
Length:329
Mass (Da):35,541
Last modified:November 1, 1997 - v2
Checksum:iA79D167EA92B0C64
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti194Q → E in CAA29476 (PubMed:2445996).Curated1
Sequence conflicti281V → L in CAA29476 (PubMed:2445996).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06091 Genomic DNA Translation: CAA29476.1
U00096 Genomic DNA Translation: AAC74971.1
AP009048 Genomic DNA Translation: BAA15721.1
K00420 Genomic DNA Translation: AAA23472.1
PIRiE64953 JGECA
RefSeqiNP_416414.1, NC_000913.3
WP_000548675.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74971; AAC74971; b1901
BAA15721; BAA15721; BAA15721
GeneIDi946409
KEGGiecj:JW1889
eco:b1901
PATRICifig|1411691.4.peg.349

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06091 Genomic DNA Translation: CAA29476.1
U00096 Genomic DNA Translation: AAC74971.1
AP009048 Genomic DNA Translation: BAA15721.1
K00420 Genomic DNA Translation: AAA23472.1
PIRiE64953 JGECA
RefSeqiNP_416414.1, NC_000913.3
WP_000548675.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ABEX-ray1.70A24-329[»]
1ABFX-ray1.90A24-329[»]
1APBX-ray1.76A24-329[»]
1BAPX-ray1.75A24-329[»]
2WRZX-ray2.20A/B24-329[»]
5ABPX-ray1.80A24-329[»]
6ABPX-ray1.67A24-329[»]
7ABPX-ray1.67A24-329[»]
8ABPX-ray1.49A24-329[»]
9ABPX-ray1.97A24-329[»]
ProteinModelPortaliP02924
SMRiP02924
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261850, 21 interactors
IntActiP02924, 5 interactors
STRINGi316385.ECDH10B_2041

Chemistry databases

DrugBankiDB03485 Alpha-D-Fucose
DB03142 Alpha-L-Arabinose
DB04062 Beta-D-Fucose
DB03246 Beta-L-Arabinose

Protein family/group databases

TCDBi3.A.1.2.2 the atp-binding cassette (abc) superfamily

Proteomic databases

EPDiP02924
PaxDbiP02924
PRIDEiP02924

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74971; AAC74971; b1901
BAA15721; BAA15721; BAA15721
GeneIDi946409
KEGGiecj:JW1889
eco:b1901
PATRICifig|1411691.4.peg.349

Organism-specific databases

EchoBASEiEB0055
EcoGeneiEG10057 araF

Phylogenomic databases

eggNOGiENOG4105DMK Bacteria
COG1879 LUCA
HOGENOMiHOG000220638
KOiK10537
PhylomeDBiP02924

Enzyme and pathway databases

BioCyciEcoCyc:ARAF-MONOMER
MetaCyc:ARAF-MONOMER

Miscellaneous databases

EvolutionaryTraceiP02924
PROiPR:P02924

Family and domain databases

CDDicd01540 PBP1_arabinose_binding, 1 hit
InterProiView protein in InterPro
IPR026266 AraF
IPR001761 Peripla_BP/Lac1_sug-bd_dom
IPR028082 Peripla_BP_I
PfamiView protein in Pfam
PF00532 Peripla_BP_1, 1 hit
PIRSFiPIRSF002816 AraF, 1 hit
SUPFAMiSSF53822 SSF53822, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiARAF_ECOLI
AccessioniPrimary (citable) accession number: P02924
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 1, 1997
Last modified: November 7, 2018
This is version 156 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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