UniProtKB - P02921 (MELB_ECOLI)
Protein
Melibiose carrier protein
Gene
melB
Organism
Escherichia coli (strain K12)
Status
Functioni
Responsible for melibiose and other galactoside transport. It is capable of using hydrogen, sodium, and lithium cations as coupling cations for cotransport, depending on the particular sugar transported (symport system).
GO - Molecular functioni
- melibiose:cation symporter activity Source: EcoCyc
- melibiose:sodium symporter activity Source: EcoCyc
- methylgalactoside transmembrane transporter activity Source: EcoCyc
GO - Biological processi
- melibiose transport Source: EcoCyc
- methylgalactoside transport Source: EcoCyc
- organic substance transport Source: GO_Central
Keywordsi
Biological process | Sugar transport, Symport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:MELB-MONOMER MetaCyc:MELB-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Melibiose carrier proteinAlternative name(s): Melibiose permease Melibiose transporter Na+ (Li+)/melibiose symporter Thiomethylgalactoside permease II |
Gene namesi | Name:melB Synonyms:mel-4 Ordered Locus Names:b4120, JW4081 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 5 – 19 | CytoplasmicCuratedAdd BLAST | 15 | |
Transmembranei | 20 – 40 | HelicalCuratedAdd BLAST | 21 | |
Topological domaini | 41 – 42 | PeriplasmicCurated | 2 | |
Transmembranei | 43 – 63 | HelicalCuratedAdd BLAST | 21 | |
Topological domaini | 64 – 80 | CytoplasmicCuratedAdd BLAST | 17 | |
Transmembranei | 81 – 101 | HelicalCuratedAdd BLAST | 21 | |
Topological domaini | 102 – 108 | PeriplasmicCurated | 7 | |
Transmembranei | 109 – 129 | HelicalCuratedAdd BLAST | 21 | |
Topological domaini | 130 – 153 | CytoplasmicCuratedAdd BLAST | 24 | |
Transmembranei | 154 – 174 | HelicalCuratedAdd BLAST | 21 | |
Topological domaini | 175 – 186 | PeriplasmicCuratedAdd BLAST | 12 | |
Transmembranei | 187 – 207 | HelicalCuratedAdd BLAST | 21 | |
Topological domaini | 208 – 235 | CytoplasmicCuratedAdd BLAST | 28 | |
Transmembranei | 236 – 256 | HelicalCuratedAdd BLAST | 21 | |
Topological domaini | 257 – 269 | PeriplasmicCuratedAdd BLAST | 13 | |
Transmembranei | 270 – 290 | HelicalCuratedAdd BLAST | 21 | |
Topological domaini | 291 – 299 | CytoplasmicCurated | 9 | |
Transmembranei | 300 – 320 | HelicalCuratedAdd BLAST | 21 | |
Topological domaini | 321 – 328 | PeriplasmicCurated | 8 | |
Transmembranei | 329 – 349 | HelicalCuratedAdd BLAST | 21 | |
Topological domaini | 350 – 381 | CytoplasmicCuratedAdd BLAST | 32 | |
Transmembranei | 382 – 402 | HelicalCuratedAdd BLAST | 21 | |
Topological domaini | 403 – 410 | PeriplasmicCurated | 8 | |
Transmembranei | 411 – 431 | HelicalCuratedAdd BLAST | 21 | |
Topological domaini | 432 – 473 | CytoplasmicCuratedAdd BLAST | 42 |
GO - Cellular componenti
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 59 | D → C: Loses ability to catalyze Na(+) or H(+)-coupled melibiose transport against a concentration gradient. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000170752 | 2 – 473 | Melibiose carrier proteinAdd BLAST | 472 |
Proteomic databases
PaxDbi | P02921 |
PRIDEi | P02921 |
Interactioni
Subunit structurei
Monomer and homodimer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4263176, 14 interactors |
DIPi | DIP-10180N |
IntActi | P02921, 1 interactor |
STRINGi | 511145.b4120 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG2211, Bacteria |
HOGENOMi | CLU_027408_0_3_6 |
InParanoidi | P02921 |
Family and domain databases
InterProi | View protein in InterPro IPR039672, MFS_2 IPR036259, MFS_trans_sf IPR001927, Na/Gal_symport IPR018043, Na/Gal_symport_CS |
PANTHERi | PTHR11328, PTHR11328, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
TIGRFAMsi | TIGR00792, gph, 1 hit |
PROSITEi | View protein in PROSITE PS00872, NA_GALACTOSIDE_SYMP, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P02921-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSISMTTKLS YGFGAFGKDF AIGIVYMYLM YYYTDVVGLS VGLVGTLFLV
60 70 80 90 100
ARIWDAINDP IMGWIVNATR SRWGKFKPWI LIGTLANSVI LFLLFSAHLF
110 120 130 140 150
EGTTQIVFVC VTYILWGMTY TIMDIPFWSL VPTITLDKRE REQLVPYPRF
160 170 180 190 200
FASLAGFVTA GVTLPFVNYV GGGDRGFGFQ MFTLVLIAFF IVSTIITLRN
210 220 230 240 250
VHEVFSSDNQ PSAEGSHLTL KAIVALIYKN DQLSCLLGMA LAYNVASNII
260 270 280 290 300
TGFAIYYFSY VIGDADLFPY YLSYAGAANL VTLVFFPRLV KSLSRRILWA
310 320 330 340 350
GASILPVLSC GVLLLMALMS YHNVVLIVIA GILLNVGTAL FWVLQVIMVA
360 370 380 390 400
DIVDYGEYKL HVRCESIAYS VQTMVVKGGS AFAAFFIAVV LGMIGYVPNV
410 420 430 440 450
EQSTQALLGM QFIMIALPTL FFMVTLILYF RFYRLNGDTL RRIQIHLLDK
460 470
YRKVPPEPVH ADIPVGAVSD VKA
Sequence cautioni
The sequence AAA24148 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence AAA97020 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence BAE78122 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K01991 Genomic DNA Translation: AAA24148.1 Different initiation. U14003 Genomic DNA Translation: AAA97020.1 Different initiation. U00096 Genomic DNA Translation: AAC77081.2 AP009048 Genomic DNA Translation: BAE78122.1 Different initiation. |
PIRi | A03421, BDEC |
RefSeqi | NP_418544.2, NC_000913.3 WP_000028111.1, NZ_SSZK01000018.1 |
Genome annotation databases
EnsemblBacteriai | AAC77081; AAC77081; b4120 BAE78122; BAE78122; BAE78122 |
GeneIDi | 948635 |
KEGGi | ecj:JW4081 eco:b4120 |
PATRICi | fig|511145.12.peg.4251 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K01991 Genomic DNA Translation: AAA24148.1 Different initiation. U14003 Genomic DNA Translation: AAA97020.1 Different initiation. U00096 Genomic DNA Translation: AAC77081.2 AP009048 Genomic DNA Translation: BAE78122.1 Different initiation. |
PIRi | A03421, BDEC |
RefSeqi | NP_418544.2, NC_000913.3 WP_000028111.1, NZ_SSZK01000018.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4263176, 14 interactors |
DIPi | DIP-10180N |
IntActi | P02921, 1 interactor |
STRINGi | 511145.b4120 |
Proteomic databases
PaxDbi | P02921 |
PRIDEi | P02921 |
Genome annotation databases
EnsemblBacteriai | AAC77081; AAC77081; b4120 BAE78122; BAE78122; BAE78122 |
GeneIDi | 948635 |
KEGGi | ecj:JW4081 eco:b4120 |
PATRICi | fig|511145.12.peg.4251 |
Organism-specific databases
EchoBASEi | EB0573 |
Phylogenomic databases
eggNOGi | COG2211, Bacteria |
HOGENOMi | CLU_027408_0_3_6 |
InParanoidi | P02921 |
Enzyme and pathway databases
BioCyci | EcoCyc:MELB-MONOMER MetaCyc:MELB-MONOMER |
Miscellaneous databases
PROi | PR:P02921 |
Family and domain databases
InterProi | View protein in InterPro IPR039672, MFS_2 IPR036259, MFS_trans_sf IPR001927, Na/Gal_symport IPR018043, Na/Gal_symport_CS |
PANTHERi | PTHR11328, PTHR11328, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
TIGRFAMsi | TIGR00792, gph, 1 hit |
PROSITEi | View protein in PROSITE PS00872, NA_GALACTOSIDE_SYMP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MELB_ECOLI | |
Accessioni | P02921Primary (citable) accession number: P02921 Secondary accession number(s): Q2M6I4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | October 19, 2011 | |
Last modified: | December 2, 2020 | |
This is version 164 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families