UniProtKB - P02919 (PBPB_ECOLI)
Penicillin-binding protein 1B
mrcB
Functioni
Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits).
Miscellaneous
Catalytic activityi
- [GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + β-D-GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = [GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H+2 PublicationsEC:2.4.1.1292 Publications
- Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.1 Publication EC:3.4.16.4
: peptidoglycan biosynthesis Pathwayi
This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 233 | Proton donor; for transglycosylase activity1 Publication | 1 | |
Active sitei | 510 | Acyl-ester intermediate; for transpeptidase activity1 Publication | 1 |
GO - Molecular functioni
- penicillin binding Source: EcoCyc
- peptidoglycan glycosyltransferase activity Source: EcoCyc
- serine-type D-Ala-D-Ala carboxypeptidase activity Source: EcoCyc
GO - Biological processi
- cell wall repair Source: EcoCyc
- peptidoglycan biosynthetic process Source: EcoCyc
- positive regulation of bipolar cell growth Source: EcoliWiki
- regulation of cell shape Source: UniProtKB-KW
- response to antibiotic Source: EcoCyc
- response to X-ray Source: EcoCyc
Keywordsi
Molecular function | Carboxypeptidase, Glycosyltransferase, Hydrolase, Multifunctional enzyme, Protease, Transferase |
Biological process | Antibiotic resistance, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10605-MONOMER |
BRENDAi | 2.4.1.129, 2026 |
UniPathwayi | UPA00219 |
Protein family/group databases
CAZyi | GT51, Glycosyltransferase Family 51 |
Names & Taxonomyi
Protein namesi | Recommended name: Penicillin-binding protein 1BShort name: PBP-1b Short name: PBP1b Alternative name(s): Murein polymerase Including the following 2 domains: |
Gene namesi | Name:mrcB Synonyms:pbpF, ponB Ordered Locus Names:b0149, JW0145 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
Cell Wall
- peptidoglycan-based cell wall Source: InterPro
Plasma Membrane
- integral component of plasma membrane Source: EcoCyc
Other locations
- membrane Source: UniProtKB
- outer membrane-bounded periplasmic space Source: EcoCyc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 63 | CytoplasmicCuratedAdd BLAST | 63 | |
Transmembranei | 64 – 87 | Helical; Signal-anchor for type II membrane proteinCuratedAdd BLAST | 24 | |
Topological domaini | 88 – 844 | PeriplasmicCuratedAdd BLAST | 757 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 233 | E → Q: Loss of wild-type glycan chain elongation activity. No complementation in strain defective in PBP-1b. 1 Publication | 1 | |
Mutagenesisi | 234 | D → N: 7-fold decrease in catalytic activity. No complementation in strain defective in PBP-1b. 1 Publication | 1 | |
Mutagenesisi | 290 | E → Q: 11-fold decrease in catalytic activity. Shows complementation activity in strain defective in PBP-1b. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL1814 |
DrugBanki | DB01602, Bacampicillin DB00578, Carbenicillin DB09319, Carindacillin DB01414, Cefacetrile DB01327, Cefazolin DB00274, Cefmetazole DB01328, Cefonicid DB01329, Cefoperazone DB01331, Cefoxitin DB00430, Cefpiramide DB11367, Cefroxadine DB00438, Ceftazidime DB01415, Ceftibuten DB01332, Ceftizoxime DB09050, Ceftolozane DB00689, Cephaloglycin DB01000, Cyclacillin DB06211, Doripenem DB00303, Ertapenem DB01598, Imipenem DB04570, Latamoxef |
DrugCentrali | P02919 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000012145 | 1 – 844 | Penicillin-binding protein 1BAdd BLAST | 844 |
Proteomic databases
jPOSTi | P02919 |
PaxDbi | P02919 |
PRIDEi | P02919 |
Interactioni
Subunit structurei
Forms a trimeric complex with MipA and MltA. Has also been shown to exist as monomer or homodimer; homodimer of Alpha and Gamma isozymes can be found.
Interacts with UvrA, FtsL and FtsN.
2 PublicationsBinary interactionsi
P02919
With | #Exp. | IntAct |
---|---|---|
ftsI [P0AD68] | 3 | EBI-909769,EBI-548564 |
ftsN [P29131] | 7 | EBI-909769,EBI-1134233 |
lpoB [P0AB38] | 4 | EBI-909769,EBI-3405489 |
Protein-protein interaction databases
BioGRIDi | 4260880, 259 interactors |
DIPi | DIP-10252N |
IntActi | P02919, 15 interactors |
STRINGi | 511145.b0149 |
Chemistry databases
BindingDBi | P02919 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P02919 |
SMRi | P02919 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P02919 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 60 | DisorderedSequence analysisAdd BLAST | 60 | |
Regioni | 88 – 250 | Membrane associationAdd BLAST | 163 | |
Regioni | 109 – 200 | UvrB domain 2 homologAdd BLAST | 92 | |
Regioni | 195 – 367 | TransglycosylaseAdd BLAST | 173 | |
Regioni | 444 – 736 | TranspeptidaseAdd BLAST | 293 | |
Regioni | 793 – 835 | DisorderedSequence analysisAdd BLAST | 43 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 793 – 828 | Polar residuesSequence analysisAdd BLAST | 36 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0744, Bacteria |
HOGENOMi | CLU_006354_2_7_6 |
InParanoidi | P02919 |
OMAi | VHGMGLA |
PhylomeDBi | P02919 |
Family and domain databases
Gene3Di | 1.10.3810.10, 1 hit 3.40.710.10, 1 hit |
InterProi | View protein in InterPro IPR012338, Beta-lactam/transpept-like IPR001264, Glyco_trans_51 IPR023346, Lysozyme-like_dom_sf IPR032730, PBP1b_TM IPR011813, PBP_1b IPR036950, PBP_transglycosylase IPR001460, PCN-bd_Tpept IPR028166, UB2H |
Pfami | View protein in Pfam PF14812, PBP1_TM, 1 hit PF00912, Transgly, 1 hit PF00905, Transpeptidase, 1 hit PF14814, UB2H, 1 hit |
PIRSFi | PIRSF002799, PBP_1b, 1 hit |
SUPFAMi | SSF53955, SSF53955, 1 hit SSF56601, SSF56601, 1 hit |
TIGRFAMsi | TIGR02071, PBP_1b, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative initiation. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAGNDREPIG RKGKPTRPVK QKVSRRRYED DDDYDDYDDY EDEEPMPRKG
60 70 80 90 100
KGKGKGRKPR GKRGWLWLLL KLAIVFAVLI AIYGVYLDQK IRSRIDGKVW
110 120 130 140 150
QLPAAVYGRM VNLEPDMTIS KNEMVKLLEA TQYRQVSKMT RPGEFTVQAN
160 170 180 190 200
SIEMIRRPFD FPDSKEGQVR ARLTFDGDHL ATIVNMENNR QFGFFRLDPR
210 220 230 240 250
LITMISSPNG EQRLFVPRSG FPDLLVDTLL ATEDRHFYEH DGISLYSIGR
260 270 280 290 300
AVLANLTAGR TVQGASTLTQ QLVKNLFLSS ERSYWRKANE AYMALIMDAR
310 320 330 340 350
YSKDRILELY MNEVYLGQSG DNEIRGFPLA SLYYFGRPVE ELSLDQQALL
360 370 380 390 400
VGMVKGASIY NPWRNPKLAL ERRNLVLRLL QQQQIIDQEL YDMLSARPLG
410 420 430 440 450
VQPRGGVISP QPAFMQLVRQ ELQAKLGDKV KDLSGVKIFT TFDSVAQDAA
460 470 480 490 500
EKAAVEGIPA LKKQRKLSDL ETAIVVVDRF SGEVRAMVGG SEPQFAGYNR
510 520 530 540 550
AMQARRSIGS LAKPATYLTA LSQPKIYRLN TWIADAPIAL RQPNGQVWSP
560 570 580 590 600
QNDDRRYSES GRVMLVDALT RSMNVPTVNL GMALGLPAVT ETWIKLGVPK
610 620 630 640 650
DQLHPVPAML LGALNLTPIE VAQAFQTIAS GGNRAPLSAL RSVIAEDGKV
660 670 680 690 700
LYQSFPQAER AVPAQAAYLT LWTMQQVVQR GTGRQLGAKY PNLHLAGKTG
710 720 730 740 750
TTNNNVDTWF AGIDGSTVTI TWVGRDNNQP TKLYGASGAM SIYQRYLANQ
760 770 780 790 800
TPTPLNLVPP EDIADMGVDY DGNFVCSGGM RILPVWTSDP QSLCQQSEMQ
810 820 830 840
QQPSGNPFDQ SSQPQQQPQQ QPAQQEQKDS DGVAGWIKDM FGSN
The sequence of this isoform differs from the canonical sequence as follows:
1-45: Missing.
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 103 | P → A (PubMed:3882429).Curated | 1 | |
Sequence conflicti | 103 | P → A (PubMed:8202364).Curated | 1 | |
Sequence conflicti | 754 | P → PTP (PubMed:8202364).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_018737 | 1 – 45 | Missing in isoform Gamma. CuratedAdd BLAST | 45 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X02163 Genomic DNA Translation: CAA26098.1 X02163 Genomic DNA Translation: CAA26099.1 Different initiation. U00096 Genomic DNA Translation: AAC73260.1 AP009048 Genomic DNA Translation: BAB96725.2 |
PIRi | E64738, ZPECPB |
RefSeqi | NP_414691.1, NC_000913.3 WP_000918162.1, NZ_STEB01000032.1 |
Genome annotation databases
EnsemblBacteriai | AAC73260; AAC73260; b0149 BAB96725; BAB96725; BAB96725 |
GeneIDi | 944843 |
KEGGi | ecj:JW0145 eco:b0149 |
PATRICi | fig|1411691.4.peg.2132 |
Keywords - Coding sequence diversityi
Alternative initiationSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X02163 Genomic DNA Translation: CAA26098.1 X02163 Genomic DNA Translation: CAA26099.1 Different initiation. U00096 Genomic DNA Translation: AAC73260.1 AP009048 Genomic DNA Translation: BAB96725.2 |
PIRi | E64738, ZPECPB |
RefSeqi | NP_414691.1, NC_000913.3 WP_000918162.1, NZ_STEB01000032.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3FWL | X-ray | 3.09 | A | 58-804 | [»] | |
3VMA | X-ray | 2.16 | A | 58-804 | [»] | |
5FGZ | X-ray | 2.85 | A | 58-804 | [»] | |
5HL9 | X-ray | 2.70 | A | 58-804 | [»] | |
5HLA | X-ray | 2.36 | A | 58-804 | [»] | |
5HLB | X-ray | 2.42 | A | 58-804 | [»] | |
5HLD | X-ray | 2.31 | A | 58-804 | [»] | |
6FZK | NMR | - | A | 108-200 | [»] | |
6G5R | NMR | - | A | 108-200 | [»] | |
6YN0 | X-ray | 2.40 | A | 58-804 | [»] | |
7LQ6 | electron microscopy | 3.28 | A | 58-804 | [»] | |
AlphaFoldDBi | P02919 | |||||
SMRi | P02919 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260880, 259 interactors |
DIPi | DIP-10252N |
IntActi | P02919, 15 interactors |
STRINGi | 511145.b0149 |
Chemistry databases
BindingDBi | P02919 |
ChEMBLi | CHEMBL1814 |
DrugBanki | DB01602, Bacampicillin DB00578, Carbenicillin DB09319, Carindacillin DB01414, Cefacetrile DB01327, Cefazolin DB00274, Cefmetazole DB01328, Cefonicid DB01329, Cefoperazone DB01331, Cefoxitin DB00430, Cefpiramide DB11367, Cefroxadine DB00438, Ceftazidime DB01415, Ceftibuten DB01332, Ceftizoxime DB09050, Ceftolozane DB00689, Cephaloglycin DB01000, Cyclacillin DB06211, Doripenem DB00303, Ertapenem DB01598, Imipenem DB04570, Latamoxef |
DrugCentrali | P02919 |
Protein family/group databases
CAZyi | GT51, Glycosyltransferase Family 51 |
Proteomic databases
jPOSTi | P02919 |
PaxDbi | P02919 |
PRIDEi | P02919 |
Genome annotation databases
EnsemblBacteriai | AAC73260; AAC73260; b0149 BAB96725; BAB96725; BAB96725 |
GeneIDi | 944843 |
KEGGi | ecj:JW0145 eco:b0149 |
PATRICi | fig|1411691.4.peg.2132 |
Organism-specific databases
EchoBASEi | EB0600 |
Phylogenomic databases
eggNOGi | COG0744, Bacteria |
HOGENOMi | CLU_006354_2_7_6 |
InParanoidi | P02919 |
OMAi | VHGMGLA |
PhylomeDBi | P02919 |
Enzyme and pathway databases
UniPathwayi | UPA00219 |
BioCyci | EcoCyc:EG10605-MONOMER |
BRENDAi | 2.4.1.129, 2026 |
Miscellaneous databases
EvolutionaryTracei | P02919 |
PROi | PR:P02919 |
Family and domain databases
Gene3Di | 1.10.3810.10, 1 hit 3.40.710.10, 1 hit |
InterProi | View protein in InterPro IPR012338, Beta-lactam/transpept-like IPR001264, Glyco_trans_51 IPR023346, Lysozyme-like_dom_sf IPR032730, PBP1b_TM IPR011813, PBP_1b IPR036950, PBP_transglycosylase IPR001460, PCN-bd_Tpept IPR028166, UB2H |
Pfami | View protein in Pfam PF14812, PBP1_TM, 1 hit PF00912, Transgly, 1 hit PF00905, Transpeptidase, 1 hit PF14814, UB2H, 1 hit |
PIRSFi | PIRSF002799, PBP_1b, 1 hit |
SUPFAMi | SSF53955, SSF53955, 1 hit SSF56601, SSF56601, 1 hit |
TIGRFAMsi | TIGR02071, PBP_1b, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PBPB_ECOLI | |
Accessioni | P02919Primary (citable) accession number: P02919 Secondary accession number(s): P75664 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 19, 2003 | |
Last modified: | May 25, 2022 | |
This is version 217 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families