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UniProtKB - P02918 (PBPA_ECOLI)
Protein
Penicillin-binding protein 1A
Gene
mrcA
Organism
Escherichia coli (strain K12)
Status
Functioni
Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits).
1 PublicationCatalytic activityi
- [GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + β-D-GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = [GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H+1 PublicationEC:2.4.1.1291 Publication
- Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.1 Publication EC:3.4.16.4
: peptidoglycan biosynthesis Pathwayi
This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.1 PublicationView all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 86 | Proton donor; for transglycosylase activityBy similarity | 1 | |
Active sitei | 465 | Acyl-ester intermediate; for transpeptidase activityBy similarity | 1 |
GO - Molecular functioni
- penicillin binding Source: EcoCyc
- peptidoglycan glycosyltransferase activity Source: EcoCyc
- serine-type D-Ala-D-Ala carboxypeptidase activity Source: EcoCyc
GO - Biological processi
- cell wall organization Source: UniProtKB-KW
- peptidoglycan biosynthetic process Source: EcoCyc
- regulation of cell shape Source: EcoCyc
- response to antibiotic Source: EcoCyc
Keywordsi
Molecular function | Carboxypeptidase, Glycosyltransferase, Hydrolase, Multifunctional enzyme, Protease, Transferase |
Biological process | Antibiotic resistance, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10748-MONOMER |
UniPathwayi | UPA00219 |
Protein family/group databases
CAZyi | GT51, Glycosyltransferase Family 51 |
Names & Taxonomyi
Protein namesi | Recommended name: Penicillin-binding protein 1AShort name: PBP-1a Short name: PBP1a Including the following 2 domains: |
Gene namesi | Name:mrcA Synonyms:ponA Ordered Locus Names:b3396, JW3359 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 1 Publication; Single-pass type II membrane protein Sequence analysis
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 5 | CytoplasmicSequence analysis | 5 | |
Transmembranei | 6 – 26 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 27 – 850 | PeriplasmicSequence analysisAdd BLAST | 824 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Chemistry databases
ChEMBLi | CHEMBL1813 |
DrugBanki | DB01602, Bacampicillin DB00578, Carbenicillin DB09319, Carindacillin DB01414, Cefacetrile DB01327, Cefazolin DB00274, Cefmetazole DB01328, Cefonicid DB01329, Cefoperazone DB01331, Cefoxitin DB00430, Cefpiramide DB01333, Cefradine DB00438, Ceftazidime DB01415, Ceftibuten DB01332, Ceftizoxime DB09050, Ceftolozane DB01000, Cyclacillin DB06211, Doripenem DB00303, Ertapenem DB01598, Imipenem DB04570, Latamoxef |
DrugCentrali | P02918 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000083164 | 1 – 850 | Penicillin-binding protein 1AAdd BLAST | 850 |
Proteomic databases
jPOSTi | P02918 |
PaxDbi | P02918 |
PRIDEi | P02918 |
Interactioni
Binary interactionsi
Protein-protein interaction databases
BioGRIDi | 4262992, 302 interactors |
ComplexPortali | CPX-5718, Elongasome complex |
DIPi | DIP-10251N |
IntActi | P02918, 9 interactors |
STRINGi | 511145.b3396 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 48 – 216 | TransglycosylaseAdd BLAST | 169 | |
Regioni | 400 – 710 | TranspeptidaseAdd BLAST | 311 |
Sequence similaritiesi
In the N-terminal section; belongs to the glycosyltransferase 51 family.Curated
In the C-terminal section; belongs to the transpeptidase family.Curated
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG5009, Bacteria |
HOGENOMi | CLU_006354_2_4_6 |
InParanoidi | P02918 |
PhylomeDBi | P02918 |
Family and domain databases
Gene3Di | 1.10.3810.10, 1 hit 3.40.710.10, 2 hits |
InterProi | View protein in InterPro IPR012338, Beta-lactam/transpept-like IPR001264, Glyco_trans_51 IPR023346, Lysozyme-like_dom_sf IPR036950, PBP_transglycosylase IPR031376, PCB_OB IPR001460, PCN-bd_Tpept |
Pfami | View protein in Pfam PF17092, PCB_OB, 1 hit PF00912, Transgly, 1 hit PF00905, Transpeptidase, 1 hit |
SUPFAMi | SSF53955, SSF53955, 1 hit SSF56601, SSF56601, 1 hit |
i Sequence
Sequence statusi: Complete.
P02918-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKFVKYFLIL AVCCILLGAG SIYGLYRYIE PQLPDVATLK DVRLQIPMQI
60 70 80 90 100
YSADGELIAQ YGEKRRIPVT LDQIPPEMVK AFIATEDSRF YEHHGVDPVG
110 120 130 140 150
IFRAASVALF SGHASQGAST ITQQLARNFF LSPERTLMRK IKEVFLAIRI
160 170 180 190 200
EQLLTKDEIL ELYLNKIYLG YRAYGVGAAA QVYFGKTVDQ LTLNEMAVIA
210 220 230 240 250
GLPKAPSTFN PLYSMDRAVA RRNVVLSRML DEGYITQQQF DQTRTEAINA
260 270 280 290 300
NYHAPEIAFS APYLSEMVRQ EMYNRYGESA YEDGYRIYTT ITRKVQQAAQ
310 320 330 340 350
QAVRNNVLDY DMRHGYRGPA NVLWKVGESA WDNNKITDTL KALPTYGPLL
360 370 380 390 400
PAAVTSANPQ QATAMLADGS TVALSMEGVR WARPYRSDTQ QGPTPRKVTD
410 420 430 440 450
VLQTGQQIWV RQVGDAWWLA QVPEVNSALV SINPQNGAVM ALVGGFDFNQ
460 470 480 490 500
SKFNRATQAL RQVGSNIKPF LYTAAMDKGL TLASMLNDVP ISRWDASAGS
510 520 530 540 550
DWQPKNSPPQ YAGPIRLRQG LGQSKNVVMV RAMRAMGVDY AAEYLQRFGF
560 570 580 590 600
PAQNIVHTES LALGSASFTP MQVARGYAVM ANGGFLVDPW FISKIENDQG
610 620 630 640 650
GVIFEAKPKV ACPECDIPVI YGDTQKSNVL ENNDVEDVAI SREQQNVSVP
660 670 680 690 700
MPQLEQANQA LVAKTGAQEY APHVINTPLA FLIKSALNTN IFGEPGWQGT
710 720 730 740 750
GWRAGRDLQR RDIGGKTGTT NSSKDAWFSG YGPGVVTSVW IGFDDHRRNL
760 770 780 790 800
GHTTASGAIK DQISGYEGGA KSAQPAWDAY MKAVLEGVPE QPLTPPPGIV
810 820 830 840 850
TVNIDRSTGQ LANGGNSREE YFIEGTQPTQ QAVHEVGTTI IDNGEAQELF
Sequence cautioni
The sequence AAA58193 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X02164 Genomic DNA Translation: CAA26100.1 U18997 Genomic DNA Translation: AAA58193.1 Different initiation. U00096 Genomic DNA Translation: AAC76421.2 AP009048 Genomic DNA Translation: BAE77895.1 |
PIRi | G65134, ZPECPA |
RefSeqi | NP_417855.4, NC_000913.3 WP_001336003.1, NZ_SSZK01000008.1 |
Genome annotation databases
EnsemblBacteriai | AAC76421; AAC76421; b3396 BAE77895; BAE77895; BAE77895 |
GeneIDi | 947907 |
KEGGi | ecj:JW3359 eco:b3396 |
PATRICi | fig|1411691.4.peg.3334 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X02164 Genomic DNA Translation: CAA26100.1 U18997 Genomic DNA Translation: AAA58193.1 Different initiation. U00096 Genomic DNA Translation: AAC76421.2 AP009048 Genomic DNA Translation: BAE77895.1 |
PIRi | G65134, ZPECPA |
RefSeqi | NP_417855.4, NC_000913.3 WP_001336003.1, NZ_SSZK01000008.1 |
3D structure databases
SMRi | P02918 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262992, 302 interactors |
ComplexPortali | CPX-5718, Elongasome complex |
DIPi | DIP-10251N |
IntActi | P02918, 9 interactors |
STRINGi | 511145.b3396 |
Chemistry databases
ChEMBLi | CHEMBL1813 |
DrugBanki | DB01602, Bacampicillin DB00578, Carbenicillin DB09319, Carindacillin DB01414, Cefacetrile DB01327, Cefazolin DB00274, Cefmetazole DB01328, Cefonicid DB01329, Cefoperazone DB01331, Cefoxitin DB00430, Cefpiramide DB01333, Cefradine DB00438, Ceftazidime DB01415, Ceftibuten DB01332, Ceftizoxime DB09050, Ceftolozane DB01000, Cyclacillin DB06211, Doripenem DB00303, Ertapenem DB01598, Imipenem DB04570, Latamoxef |
DrugCentrali | P02918 |
Protein family/group databases
CAZyi | GT51, Glycosyltransferase Family 51 |
Proteomic databases
jPOSTi | P02918 |
PaxDbi | P02918 |
PRIDEi | P02918 |
Genome annotation databases
EnsemblBacteriai | AAC76421; AAC76421; b3396 BAE77895; BAE77895; BAE77895 |
GeneIDi | 947907 |
KEGGi | ecj:JW3359 eco:b3396 |
PATRICi | fig|1411691.4.peg.3334 |
Organism-specific databases
EchoBASEi | EB0741 |
Phylogenomic databases
eggNOGi | COG5009, Bacteria |
HOGENOMi | CLU_006354_2_4_6 |
InParanoidi | P02918 |
PhylomeDBi | P02918 |
Enzyme and pathway databases
UniPathwayi | UPA00219 |
BioCyci | EcoCyc:EG10748-MONOMER |
Miscellaneous databases
PROi | PR:P02918 |
Family and domain databases
Gene3Di | 1.10.3810.10, 1 hit 3.40.710.10, 2 hits |
InterProi | View protein in InterPro IPR012338, Beta-lactam/transpept-like IPR001264, Glyco_trans_51 IPR023346, Lysozyme-like_dom_sf IPR036950, PBP_transglycosylase IPR031376, PCB_OB IPR001460, PCN-bd_Tpept |
Pfami | View protein in Pfam PF17092, PCB_OB, 1 hit PF00912, Transgly, 1 hit PF00905, Transpeptidase, 1 hit |
SUPFAMi | SSF53955, SSF53955, 1 hit SSF56601, SSF56601, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PBPA_ECOLI | |
Accessioni | P02918Primary (citable) accession number: P02918 Secondary accession number(s): P76688, Q2M761 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | February 23, 2022 | |
This is version 196 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families