UniProtKB - P02916 (MALF_ECOLI)
Protein
Maltose/maltodextrin transport system permease protein MalF
Gene
malF
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of the ABC transporter complex MalEFGK involved in maltose/maltodextrin import. Probably responsible for the translocation of the substrate across the membrane.2 Publications
Miscellaneous
When MalF EAA loop mutations are made concomitantly with MalG EAA loop mutations, a complete loss of transport and complex formation is observed, except for the Gly-407. This suggests that the MalF-MalG interaction may be important for the proper assembly and also for the correct function of the transporter.
GO - Molecular functioni
- ABC-type maltose transporter activity Source: EcoCyc
GO - Biological processi
- cellular response to DNA damage stimulus Source: EcoliWiki
- maltodextrin transport Source: EcoCyc
- maltose transport Source: EcoCyc
Keywordsi
Biological process | Sugar transport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:MALF-MONOMER MetaCyc:MALF-MONOMER |
Protein family/group databases
TCDBi | 3.A.1.1.1, the atp-binding cassette (abc) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Maltose/maltodextrin transport system permease protein MalFCurated |
Gene namesi | Name:malF1 Publication Ordered Locus Names:b4033, JW3993 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 1 Publication; Multi-pass membrane protein PROSITE-ProRule annotation1 Publication; Periplasmic side 1 Publication
Note: A substantial portion of it protrudes into the periplasmic space; inserts in an SRP- and Sec-dependent, YidC-independent fashion into the membrane.
Plasma Membrane
- ATP-binding cassette (ABC) transporter complex Source: EcoCyc
- integral component of plasma membrane Source: EcoCyc
- maltose transport complex Source: EcoCyc
- plasma membrane Source: EcoCyc
Other locations
- membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 16 | CytoplasmicCuratedAdd BLAST | 16 | |
Transmembranei | 17 – 36 | HelicalCuratedAdd BLAST | 20 | |
Topological domaini | 37 – 39 | PeriplasmicCurated | 3 | |
Transmembranei | 40 – 58 | HelicalCuratedAdd BLAST | 19 | |
Topological domaini | 59 – 66 | CytoplasmicCurated | 8 | |
Transmembranei | 67 – 92 | HelicalCuratedAdd BLAST | 26 | |
Topological domaini | 93 – 275 | PeriplasmicCuratedAdd BLAST | 183 | |
Transmembranei | 276 – 306 | HelicalCuratedAdd BLAST | 31 | |
Topological domaini | 307 – 318 | CytoplasmicCuratedAdd BLAST | 12 | |
Transmembranei | 319 – 336 | HelicalCuratedAdd BLAST | 18 | |
Topological domaini | 337 – 369 | PeriplasmicCuratedAdd BLAST | 33 | |
Transmembranei | 370 – 392 | HelicalCuratedAdd BLAST | 23 | |
Topological domaini | 393 – 425 | CytoplasmicCuratedAdd BLAST | 33 | |
Transmembranei | 426 – 452 | HelicalCuratedAdd BLAST | 27 | |
Topological domaini | 453 – 483 | PeriplasmicCuratedAdd BLAST | 31 | |
Transmembranei | 484 – 505 | HelicalCuratedAdd BLAST | 22 | |
Topological domaini | 506 – 514 | CytoplasmicCurated | 9 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 334 | L → W: Ability to transport lactose in a saturable manner. 1 Publication | 1 | |
Mutagenesisi | 372 | L → W: Growth on maltose but not on media containing either maltoheptaose or maltoheptaose plus maltose. 1 Publication | 1 | |
Mutagenesisi | 376 | N → K or H: No growth on maltose. 1 Publication | 1 | |
Mutagenesisi | 380 | G → C or S: No growth on maltose. 1 Publication | 1 | |
Mutagenesisi | 401 | E → A, C, K or L: Reduction of transport rate. 2 Publications | 1 | |
Mutagenesisi | 403 | S → C, D, K or L: Reduction of transport rate. 2 Publications | 1 | |
Mutagenesisi | 407 | G → A or P: No effect. 1 Publication | 1 | |
Mutagenesisi | 420 | P → A: No effect. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000060068 | 1 – 514 | Maltose/maltodextrin transport system permease protein MalFAdd BLAST | 514 |
Proteomic databases
jPOSTi | P02916 |
PaxDbi | P02916 |
PRIDEi | P02916 |
Interactioni
Subunit structurei
The complex is composed of two ATP-binding proteins (MalK), two transmembrane proteins (MalG and MalF) and a solute-binding protein (MalE). Protein stability and stable complex formation require YidC.
4 PublicationsBinary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 4261701, 15 interactors |
ComplexPortali | CPX-1932, Maltose ABC transporter complex CPX-1978, Enzyme IIA-maltose transport inhibitory complex |
DIPi | DIP-10142N |
IntActi | P02916, 7 interactors |
STRINGi | 511145.b4033 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P02916 |
SMRi | P02916 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P02916 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 281 – 505 | ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST | 225 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG1175, Bacteria |
HOGENOMi | CLU_016047_20_0_6 |
InParanoidi | P02916 |
PhylomeDBi | P02916 |
Family and domain databases
CDDi | cd06261, TM_PBP2, 1 hit |
Gene3Di | 1.10.3720.10, 1 hit 1.20.58.370, 1 hit |
InterProi | View protein in InterPro IPR030156, MalF-like IPR035277, MalF_N IPR029345, MalF_P2 IPR000515, MetI-like IPR035906, MetI-like_sf |
PANTHERi | PTHR47314:SF1, PTHR47314:SF1, 1 hit |
Pfami | View protein in Pfam PF00528, BPD_transp_1, 1 hit PF14785, MalF_P2, 1 hit |
SUPFAMi | SSF161098, SSF161098, 1 hit |
PROSITEi | View protein in PROSITE PS50928, ABC_TM1, 1 hit |
i Sequence
Sequence statusi: Complete.
P02916-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDVIKKKHWW QSDALKWSVL GLLGLLVGYL VVLMYAQGEY LFAITTLILS
60 70 80 90 100
SAGLYIFANR KAYAWRYVYP GMAGMGLFVL FPLVCTIAIA FTNYSSTNQL
110 120 130 140 150
TFERAQEVLL DRSWQAGKTY NFGLYPAGDE WQLALSDGET GKNYLSDAFK
160 170 180 190 200
FGGEQKLQLK ETTAQPEGER ANLRVITQNR QALSDITAIL PDGNKVMMSS
210 220 230 240 250
LRQFSGTQPL YTLDGDGTLT NNQSGVKYRP NNQIGFYQSI TADGNWGDEK
260 270 280 290 300
LSPGYTVTTG WKNFTRVFTD EGIQKPFLAI FVWTVVFSLI TVFLTVAVGM
310 320 330 340 350
VLACLVQWEA LRGKAVYRVL LILPYAVPSF ISILIFKGLF NQSFGEINMM
360 370 380 390 400
LSALFGVKPA WFSDPTTART MLIIVNTWLG YPYMMILCMG LLKAIPDDLY
410 420 430 440 450
EASAMDGAGP FQNFFKITLP LLIKPLTPLM IASFAFNFNN FVLIQLLTNG
460 470 480 490 500
GPDRLGTTTP AGYTDLLVNY TYRIAFEGGG GQDFGLAAAI ATLIFLLVGA
510
LAIVNLKATR MKFD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J01648 Genomic DNA Translation: AAB59055.1 U00006 Genomic DNA Translation: AAC43127.1 U00096 Genomic DNA Translation: AAC77003.1 AP009048 Genomic DNA Translation: BAE78035.1 X02871 Genomic DNA Translation: CAA26627.1 |
PIRi | A03414, MMECMF |
RefSeqi | NP_418457.1, NC_000913.3 WP_001297290.1, NZ_STEB01000022.1 |
Genome annotation databases
EnsemblBacteriai | AAC77003; AAC77003; b4033 BAE78035; BAE78035; BAE78035 |
GeneIDi | 58390124 948532 |
KEGGi | ecj:JW3993 eco:b4033 |
PATRICi | fig|511145.12.peg.4148 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J01648 Genomic DNA Translation: AAB59055.1 U00006 Genomic DNA Translation: AAC43127.1 U00096 Genomic DNA Translation: AAC77003.1 AP009048 Genomic DNA Translation: BAE78035.1 X02871 Genomic DNA Translation: CAA26627.1 |
PIRi | A03414, MMECMF |
RefSeqi | NP_418457.1, NC_000913.3 WP_001297290.1, NZ_STEB01000022.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2R6G | X-ray | 2.80 | F | 1-514 | [»] | |
3FH6 | X-ray | 4.50 | F/H | 36-514 | [»] | |
3PUV | X-ray | 2.40 | F | 1-514 | [»] | |
3PUW | X-ray | 2.30 | F | 1-514 | [»] | |
3PUX | X-ray | 2.30 | F | 1-514 | [»] | |
3PUY | X-ray | 3.10 | F | 1-514 | [»] | |
3PUZ | X-ray | 2.90 | F | 1-514 | [»] | |
3PV0 | X-ray | 3.10 | F | 1-514 | [»] | |
3RLF | X-ray | 2.20 | F | 1-514 | [»] | |
4JBW | X-ray | 3.91 | F/H | 1-514 | [»] | |
4KHZ | X-ray | 2.90 | F | 1-514 | [»] | |
4KI0 | X-ray | 2.38 | F | 1-514 | [»] | |
BMRBi | P02916 | |||||
SMRi | P02916 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261701, 15 interactors |
ComplexPortali | CPX-1932, Maltose ABC transporter complex CPX-1978, Enzyme IIA-maltose transport inhibitory complex |
DIPi | DIP-10142N |
IntActi | P02916, 7 interactors |
STRINGi | 511145.b4033 |
Protein family/group databases
TCDBi | 3.A.1.1.1, the atp-binding cassette (abc) superfamily |
Proteomic databases
jPOSTi | P02916 |
PaxDbi | P02916 |
PRIDEi | P02916 |
Genome annotation databases
EnsemblBacteriai | AAC77003; AAC77003; b4033 BAE78035; BAE78035; BAE78035 |
GeneIDi | 58390124 948532 |
KEGGi | ecj:JW3993 eco:b4033 |
PATRICi | fig|511145.12.peg.4148 |
Organism-specific databases
EchoBASEi | EB0550 |
Phylogenomic databases
eggNOGi | COG1175, Bacteria |
HOGENOMi | CLU_016047_20_0_6 |
InParanoidi | P02916 |
PhylomeDBi | P02916 |
Enzyme and pathway databases
BioCyci | EcoCyc:MALF-MONOMER MetaCyc:MALF-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P02916 |
PROi | PR:P02916 |
Family and domain databases
CDDi | cd06261, TM_PBP2, 1 hit |
Gene3Di | 1.10.3720.10, 1 hit 1.20.58.370, 1 hit |
InterProi | View protein in InterPro IPR030156, MalF-like IPR035277, MalF_N IPR029345, MalF_P2 IPR000515, MetI-like IPR035906, MetI-like_sf |
PANTHERi | PTHR47314:SF1, PTHR47314:SF1, 1 hit |
Pfami | View protein in Pfam PF00528, BPD_transp_1, 1 hit PF14785, MalF_P2, 1 hit |
SUPFAMi | SSF161098, SSF161098, 1 hit |
PROSITEi | View protein in PROSITE PS50928, ABC_TM1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MALF_ECOLI | |
Accessioni | P02916Primary (citable) accession number: P02916 Secondary accession number(s): Q2M6S1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | April 7, 2021 | |
This is version 180 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families