UniProtKB - P02853 (PHSB_PHAVU)
Protein
Phaseolin, beta-type
Gene
N/A
Organism
Phaseolus vulgaris (Kidney bean) (French bean)
Status
Functioni
Major seed storage protein.
GO - Molecular functioni
- nutrient reservoir activity Source: UniProtKB-KW
Keywordsi
Molecular function | Seed storage protein, Storage protein |
Names & Taxonomyi
Protein namesi | Recommended name: Phaseolin, beta-type |
Organismi | Phaseolus vulgaris (Kidney bean) (French bean) |
Taxonomic identifieri | 3885 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › fabids › Fabales › Fabaceae › Papilionoideae › 50 kb inversion clade › NPAAA clade › indigoferoid/millettioid clade › Phaseoleae › Phaseolus |
Subcellular locationi
Vacuole
Note: Cotyledonary membrane-bound vacuolar protein bodies.
Vacuole
- vacuole Source: UniProtKB-SubCell
Other locations
- aleurone grain Source: UniProtKB-SubCell
Keywords - Cellular componenti
VacuolePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 24 | Sequence analysisAdd BLAST | 24 | |
ChainiPRO_0000032195 | 25 – 421 | Phaseolin, beta-typeAdd BLAST | 397 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
GlycosylationiCAR_000078 | 252 | N-linked (GlcNAc...) asparagine | 1 | |
GlycosylationiCAR_000079 | 341 | N-linked (GlcNAc...) asparagine | 1 |
Keywords - PTMi
GlycoproteinPTM databases
GlyConnecti | 494, 3 N-Linked glycans (2 sites) |
UniCarbKBi | P02853 |
Interactioni
Subunit structurei
Homotrimer that associates to form a dodecamer.
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P02853 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P02853 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 237 – 390 | Cupin type-1Sequence analysisAdd BLAST | 154 |
Sequence similaritiesi
Belongs to the 7S seed storage protein family.Curated
Keywords - Domaini
SignalFamily and domain databases
Gene3Di | 2.60.120.10, 2 hits |
InterProi | View protein in InterPro IPR006045, Cupin_1 IPR014710, RmlC-like_jellyroll IPR011051, RmlC_Cupin_sf |
Pfami | View protein in Pfam PF00190, Cupin_1, 1 hit |
SMARTi | View protein in SMART SM00835, Cupin_1, 1 hit |
SUPFAMi | SSF51182, SSF51182, 2 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P02853-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMRARVPLLL LGILFLASLS ASFATSLREE EESQDNPFYF NSDNSWNTLF
60 70 80 90 100
KNQYGHIRVL QRFDQQSKRL QNLEDYRLVE FRSKPETLLL PQQADAELLL
110 120 130 140 150
VVRSGSAILV LVKPDDRREY FFLTSDNPIF SDHQKIPAGT IFYLVNPDPK
160 170 180 190 200
EDLRIIQLAM PVNNPQIHEF FLSSTEAQQS YLQEFSKHIL EASFNSKFEE
210 220 230 240 250
INRVLFEEEG QQEGVIVNID SEQIKELSKH AKSSSRKSLS KQDNTIGNEF
260 270 280 290 300
GNLTERTDNS LNVLISSIEM EEGALFVPHY YSKAIVILVV NEGEAHVELV
310 320 330 340 350
GPKGNKETLE YESYRAELSK DDVFVIPAAY PVAIKATSNV NFTGFGINAN
360 370 380 390 400
NNNRNLLAGK TDNVISSIGR ALDGKDVLGL TFSGSGDEVM KLINKQSGSY
410 420
FVDAHHHQQE QQKGRKGAFV Y
Sequence cautioni
The sequence AAC23610 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAA24492 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 194 – 195 | FN → SM in AAP31811 (PubMed:7862642).Curated | 2 | |
Sequence conflicti | 205 | L → P in CAA24492 (Ref. 4) Curated | 1 | |
Sequence conflicti | 227 | L → Q in CAA24492 (Ref. 4) Curated | 1 | |
Sequence conflicti | 239 | L → P in CAA24492 (Ref. 4) Curated | 1 | |
Sequence conflicti | 258 | D → G in CAA24492 (Ref. 4) Curated | 1 | |
Sequence conflicti | 311 | Y → I in CAA26789 (PubMed:2997710).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J01263 Genomic DNA Translation: AAC23610.1 Sequence problems. X03004 mRNA Translation: CAA26789.1 V01163 Genomic DNA Translation: CAA24492.1 Different initiation. S74637 Genomic DNA Translation: AAP31811.1 |
PIRi | A03343, FSFB A24810 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J01263 Genomic DNA Translation: AAC23610.1 Sequence problems. X03004 mRNA Translation: CAA26789.1 V01163 Genomic DNA Translation: CAA24492.1 Different initiation. S74637 Genomic DNA Translation: AAP31811.1 |
PIRi | A03343, FSFB A24810 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1PHS | X-ray | 3.00 | A | 25-421 | [»] | |
2PHL | X-ray | 2.20 | A/B/C | 25-421 | [»] | |
SMRi | P02853 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein family/group databases
Allergomei | 8837, Pha v Phaseolin |
PTM databases
GlyConnecti | 494, 3 N-Linked glycans (2 sites) |
UniCarbKBi | P02853 |
Miscellaneous databases
EvolutionaryTracei | P02853 |
Family and domain databases
Gene3Di | 2.60.120.10, 2 hits |
InterProi | View protein in InterPro IPR006045, Cupin_1 IPR014710, RmlC-like_jellyroll IPR011051, RmlC_Cupin_sf |
Pfami | View protein in Pfam PF00190, Cupin_1, 1 hit |
SMARTi | View protein in SMART SM00835, Cupin_1, 1 hit |
SUPFAMi | SSF51182, SSF51182, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PHSB_PHAVU | |
Accessioni | P02853Primary (citable) accession number: P02853 Secondary accession number(s): P07220, Q84N11 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | January 1, 1990 | |
Last modified: | December 2, 2020 | |
This is version 117 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families