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Protein

Phaseolin, beta-type

Gene
N/A
Organism
Phaseolus vulgaris (Kidney bean) (French bean)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Major seed storage protein.

GO - Molecular functioni

Keywordsi

Molecular functionSeed storage protein, Storage protein

Names & Taxonomyi

Protein namesi
Recommended name:
Phaseolin, beta-type
OrganismiPhaseolus vulgaris (Kidney bean) (French bean)
Taxonomic identifieri3885 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideae50 kb inversion cladeNPAAA cladeindigoferoid/millettioid cladePhaseoleaePhaseolus

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Vacuole

Pathology & Biotechi

Protein family/group databases

Allergomei8837 Pha v Phaseolin

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000003219525 – 421Phaseolin, beta-typeAdd BLAST397

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
GlycosylationiCAR_000078252N-linked (GlcNAc...) asparagine1
GlycosylationiCAR_000079341N-linked (GlcNAc...) asparagine1

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP02853

PTM databases

GlyConnecti494
UniCarbKBiP02853

Interactioni

Subunit structurei

Homotrimer that associates to form a dodecamer.

Structurei

Secondary structure

1421
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP02853
SMRiP02853
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02853

Family & Domainsi

Sequence similaritiesi

Belongs to the 7S seed storage protein family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.120.10, 2 hits
InterProiView protein in InterPro
IPR006045 Cupin_1
IPR014710 RmlC-like_jellyroll
IPR011051 RmlC_Cupin_sf
PfamiView protein in Pfam
PF00190 Cupin_1, 1 hit
SMARTiView protein in SMART
SM00835 Cupin_1, 1 hit
SUPFAMiSSF51182 SSF51182, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02853-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MMRARVPLLL LGILFLASLS ASFATSLREE EESQDNPFYF NSDNSWNTLF
60 70 80 90 100
KNQYGHIRVL QRFDQQSKRL QNLEDYRLVE FRSKPETLLL PQQADAELLL
110 120 130 140 150
VVRSGSAILV LVKPDDRREY FFLTSDNPIF SDHQKIPAGT IFYLVNPDPK
160 170 180 190 200
EDLRIIQLAM PVNNPQIHEF FLSSTEAQQS YLQEFSKHIL EASFNSKFEE
210 220 230 240 250
INRVLFEEEG QQEGVIVNID SEQIKELSKH AKSSSRKSLS KQDNTIGNEF
260 270 280 290 300
GNLTERTDNS LNVLISSIEM EEGALFVPHY YSKAIVILVV NEGEAHVELV
310 320 330 340 350
GPKGNKETLE YESYRAELSK DDVFVIPAAY PVAIKATSNV NFTGFGINAN
360 370 380 390 400
NNNRNLLAGK TDNVISSIGR ALDGKDVLGL TFSGSGDEVM KLINKQSGSY
410 420
FVDAHHHQQE QQKGRKGAFV Y
Length:421
Mass (Da):47,565
Last modified:January 1, 1990 - v2
Checksum:i3197A82D90FB3D61
GO

Sequence cautioni

The sequence AAC23610 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAA24492 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti194 – 195FN → SM in AAP31811 (PubMed:7862642).Curated2
Sequence conflicti205L → P in CAA24492 (Ref. 4) Curated1
Sequence conflicti227L → Q in CAA24492 (Ref. 4) Curated1
Sequence conflicti239L → P in CAA24492 (Ref. 4) Curated1
Sequence conflicti258D → G in CAA24492 (Ref. 4) Curated1
Sequence conflicti311Y → I in CAA26789 (PubMed:2997710).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01263 Genomic DNA Translation: AAC23610.1 Sequence problems.
X03004 mRNA Translation: CAA26789.1
V01163 Genomic DNA Translation: CAA24492.1 Different initiation.
S74637 Genomic DNA Translation: AAP31811.1
PIRiA03343 FSFB
A24810

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01263 Genomic DNA Translation: AAC23610.1 Sequence problems.
X03004 mRNA Translation: CAA26789.1
V01163 Genomic DNA Translation: CAA24492.1 Different initiation.
S74637 Genomic DNA Translation: AAP31811.1
PIRiA03343 FSFB
A24810

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PHSX-ray3.00A25-421[»]
2PHLX-ray2.20A/B/C25-421[»]
ProteinModelPortaliP02853
SMRiP02853
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei8837 Pha v Phaseolin

PTM databases

GlyConnecti494
UniCarbKBiP02853

Proteomic databases

PRIDEiP02853

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP02853

Family and domain databases

Gene3Di2.60.120.10, 2 hits
InterProiView protein in InterPro
IPR006045 Cupin_1
IPR014710 RmlC-like_jellyroll
IPR011051 RmlC_Cupin_sf
PfamiView protein in Pfam
PF00190 Cupin_1, 1 hit
SMARTiView protein in SMART
SM00835 Cupin_1, 1 hit
SUPFAMiSSF51182 SSF51182, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiPHSB_PHAVU
AccessioniPrimary (citable) accession number: P02853
Secondary accession number(s): P07220, Q84N11
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 1, 1990
Last modified: September 12, 2018
This is version 110 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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