UniProtKB - P02836 (HMEN_DROME)
Protein
Segmentation polarity homeobox protein engrailed
Gene
en
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
This protein specifies the body segmentation pattern. It is required for the development of the central nervous system. Transcriptional regulator that represses activated promoters. Wg signaling operates by inactivating the SGG repression of EN autoactivation.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 454 – 513 | HomeoboxPROSITE-ProRule annotationAdd BLAST | 60 |
GO - Molecular functioni
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: FlyBase
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: FlyBase
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: FlyBase
- sequence-specific DNA binding Source: FlyBase
GO - Biological processi
- analia development Source: FlyBase
- anterior/posterior lineage restriction, imaginal disc Source: FlyBase
- anterior head segmentation Source: FlyBase
- axon guidance Source: FlyBase
- compartment pattern specification Source: FlyBase
- genital disc anterior/posterior pattern formation Source: FlyBase
- genital disc development Source: FlyBase
- gonad development Source: FlyBase
- imaginal disc-derived wing vein specification Source: FlyBase
- negative regulation of gene expression Source: FlyBase
- negative regulation of neuron apoptotic process Source: FlyBase
- negative regulation of transcription by RNA polymerase II Source: FlyBase
- neuroblast fate determination Source: FlyBase
- neuron differentiation Source: GO_Central
- positive regulation of transcription by RNA polymerase II Source: FlyBase
- posterior compartment specification Source: FlyBase
- posterior head segmentation Source: FlyBase
- regulation of gene expression Source: FlyBase
- regulation of transcription by RNA polymerase II Source: GO_Central
- segment polarity determination Source: FlyBase
- spiracle morphogenesis, open tracheal system Source: FlyBase
- trunk segmentation Source: FlyBase
- ventral midline development Source: FlyBase
- wing disc anterior/posterior pattern formation Source: FlyBase
- wing disc morphogenesis Source: FlyBase
Keywordsi
Molecular function | Developmental protein, DNA-binding, Repressor, Segmentation polarity protein |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
SignaLinki | P02836 |
Names & Taxonomyi
Protein namesi | Recommended name: Segmentation polarity homeobox protein engrailed |
Gene namesi | Name:en ORF Names:CG9015 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0000577, en |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation1 Publication
Nucleus
- nucleus Source: FlyBase
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 508 | K → Q: Reduced specificity and affinity for DNA. | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000196077 | 1 – 552 | Segmentation polarity homeobox protein engrailedAdd BLAST | 552 |
Post-translational modificationi
Phosphorylated. Phosphorylation may directly or allosterically modify its function.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P02836 |
PTM databases
iPTMneti | P02836 |
Expressioni
Developmental stagei
Expression initiates prior to the ninth embryonic nuclear division cycle within 1.5 hours after fertilization. By the cellular blastoderm stage (the 14th nuclear division cycle) is localized into 14 stripes, 1-2 cells wide, spaced along the anterior-posterior axis of the embryo.
Gene expression databases
Bgeei | FBgn0000577, Expressed in embryo and 65 other tissues |
ExpressionAtlasi | P02836, baseline and differential |
Genevisiblei | P02836, DM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 62028, 29 interactors |
ELMi | P02836 |
IntActi | P02836, 5 interactors |
STRINGi | 7227.FBpp0087197 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P02836 |
SMRi | P02836 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P02836 |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 19 – 53 | Gln-richAdd BLAST | 35 | |
Compositional biasi | 55 – 87 | Ala-richAdd BLAST | 33 | |
Compositional biasi | 232 – 240 | Ala-rich | 9 | |
Compositional biasi | 320 – 411 | Ser-richAdd BLAST | 92 |
Sequence similaritiesi
Belongs to the engrailed homeobox family.Curated
Keywords - Domaini
HomeoboxPhylogenomic databases
eggNOGi | KOG0493, Eukaryota |
GeneTreei | ENSGT00940000167868 |
HOGENOMi | CLU_485085_0_0_1 |
InParanoidi | P02836 |
OMAi | NEMWPAW |
OrthoDBi | 858478at2759 |
PhylomeDBi | P02836 |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
InterProi | View protein in InterPro IPR019549, Homeobox-engrailed_C-terminal IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR000747, Homeobox_engrailed IPR020479, Homeobox_metazoa IPR019737, Homoebox-engrailed_CS IPR000047, HTH_motif |
Pfami | View protein in Pfam PF10525, Engrail_1_C_sig, 1 hit PF00046, Homeodomain, 1 hit |
PRINTSi | PR00026, ENGRAILED PR00024, HOMEOBOX PR00031, HTHREPRESSR |
SMARTi | View protein in SMART SM00389, HOX, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS00033, ENGRAILED, 1 hit PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P02836-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALEDRCSPQ SAPSPITLQM QHLHHQQQQQ QQQQQQMQHL HQLQQLQQLH
60 70 80 90 100
QQQLAAGVFH HPAMAFDAAA AAAAAAAAAA AHAHAAALQQ RLSGSGSPAS
110 120 130 140 150
CSTPASSTPL TIKEEESDSV IGDMSFHNQT HTTNEEEEAE EDDDIDVDVD
160 170 180 190 200
DTSAGGRLPP PAHQQQSTAK PSLAFSISNI LSDRFGDVQK PGKSMENQAS
210 220 230 240 250
IFRPFEASRS QTATPSAFTR VDLLEFSRQQ QAAAAAATAA MMLERANFLN
260 270 280 290 300
CFNPAAYPRI HEEIVQSRLR RSAANAVIPP PMSSKMSDAN PEKSALGSLC
310 320 330 340 350
KAVSQIGQPA APTMTQPPLS SSASSLASPP PASNASTISS TSSVATSSSS
360 370 380 390 400
SSSGCSSAAS SLNSSPSSRL GASGSGVNAS SPQPQPIPPP SAVSRDSGME
410 420 430 440 450
SSDDTRSETG STTTEGGKNE MWPAWVYCTR YSDRPSSGPR YRRPKQPKDK
460 470 480 490 500
TNDEKRPRTA FSSEQLARLK REFNENRYLT ERRRQQLSSE LGLNEAQIKI
510 520 530 540 550
WFQNKRAKIK KSTGSKNPLA LQLMAQGLYN HTTVPLTKEE EELEMRMNGQ
IP
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 195 | M → I in AAA65478 (PubMed:3917855).Curated | 1 | |
Sequence conflicti | 208 | S → N in AAA65478 (PubMed:3917855).Curated | 1 | |
Sequence conflicti | 486 | Q → E in CAA25906 (PubMed:2481829).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M10017 mRNA Translation: AAA65478.1 K03055 Genomic DNA No translation available. K03056 Genomic DNA No translation available. AE013599 Genomic DNA Translation: AAF58639.1 AY069448 mRNA Translation: AAL39593.1 X01765 Genomic DNA Translation: CAA25906.1 |
PIRi | A90862, WJFFEN |
RefSeqi | NP_523700.2, NM_078976.4 NP_725059.1, NM_165841.2 |
Genome annotation databases
EnsemblMetazoai | FBtr0088095; FBpp0087197; FBgn0000577 FBtr0088096; FBpp0087198; FBgn0000577 |
GeneIDi | 36240 |
KEGGi | dme:Dmel_CG9015 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M10017 mRNA Translation: AAA65478.1 K03055 Genomic DNA No translation available. K03056 Genomic DNA No translation available. AE013599 Genomic DNA Translation: AAF58639.1 AY069448 mRNA Translation: AAL39593.1 X01765 Genomic DNA Translation: CAA25906.1 |
PIRi | A90862, WJFFEN |
RefSeqi | NP_523700.2, NM_078976.4 NP_725059.1, NM_165841.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1DU0 | X-ray | 2.00 | A/B | 456-512 | [»] | |
1ENH | X-ray | 2.10 | A | 456-509 | [»] | |
1HDD | X-ray | 2.80 | C/D | 453-512 | [»] | |
1P7I | X-ray | 2.10 | A/B/C/D | 454-512 | [»] | |
1P7J | X-ray | 2.10 | A/B/C/D | 454-512 | [»] | |
1ZTR | NMR | - | A | 453-512 | [»] | |
2HDD | X-ray | 1.90 | A/B | 454-512 | [»] | |
2HOS | X-ray | 1.90 | A/B | 453-513 | [»] | |
2HOT | X-ray | 2.19 | A/B | 453-513 | [»] | |
2JWT | NMR | - | A | 453-512 | [»] | |
2P81 | NMR | - | A | 469-512 | [»] | |
3HDD | X-ray | 2.20 | A/B | 454-513 | [»] | |
6FVC | NMR | - | A | 454-512 | [»] | |
6M3D | X-ray | 1.60 | C | 453-512 | [»] | |
BMRBi | P02836 | |||||
SMRi | P02836 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 62028, 29 interactors |
ELMi | P02836 |
IntActi | P02836, 5 interactors |
STRINGi | 7227.FBpp0087197 |
PTM databases
iPTMneti | P02836 |
Proteomic databases
PaxDbi | P02836 |
Genome annotation databases
EnsemblMetazoai | FBtr0088095; FBpp0087197; FBgn0000577 FBtr0088096; FBpp0087198; FBgn0000577 |
GeneIDi | 36240 |
KEGGi | dme:Dmel_CG9015 |
Organism-specific databases
CTDi | 36240 |
FlyBasei | FBgn0000577, en |
Phylogenomic databases
eggNOGi | KOG0493, Eukaryota |
GeneTreei | ENSGT00940000167868 |
HOGENOMi | CLU_485085_0_0_1 |
InParanoidi | P02836 |
OMAi | NEMWPAW |
OrthoDBi | 858478at2759 |
PhylomeDBi | P02836 |
Enzyme and pathway databases
SignaLinki | P02836 |
Miscellaneous databases
BioGRID-ORCSi | 36240, 0 hits in 3 CRISPR screens |
EvolutionaryTracei | P02836 |
GenomeRNAii | 36240 |
PROi | PR:P02836 |
Gene expression databases
Bgeei | FBgn0000577, Expressed in embryo and 65 other tissues |
ExpressionAtlasi | P02836, baseline and differential |
Genevisiblei | P02836, DM |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
InterProi | View protein in InterPro IPR019549, Homeobox-engrailed_C-terminal IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR000747, Homeobox_engrailed IPR020479, Homeobox_metazoa IPR019737, Homoebox-engrailed_CS IPR000047, HTH_motif |
Pfami | View protein in Pfam PF10525, Engrail_1_C_sig, 1 hit PF00046, Homeodomain, 1 hit |
PRINTSi | PR00026, ENGRAILED PR00024, HOMEOBOX PR00031, HTHREPRESSR |
SMARTi | View protein in SMART SM00389, HOX, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS00033, ENGRAILED, 1 hit PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HMEN_DROME | |
Accessioni | P02836Primary (citable) accession number: P02836 Secondary accession number(s): P02837 Q9V601 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | February 2, 2004 | |
Last modified: | April 7, 2021 | |
This is version 218 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families