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Protein

Heat shock protein 83

Gene

Hsp83

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Together with Hop and piwi, mediates canalization, also known as developmental robustness, likely via epigenetic silencing of existing genetic variants and suppression of transposon-induced new genetic variation. Required for piRNA biogenesis by facilitating loading of piRNAs into PIWI proteins.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39ATPBy similarity1
Binding sitei81ATPBy similarity1
Binding sitei100ATPBy similarity1
Binding sitei126ATP; via amide nitrogenBy similarity1
Binding sitei385ATPBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • TPR domain binding Source: UniProtKB
  • unfolded protein binding Source: FlyBase

GO - Biological processi

  • cellular response to heat Source: FlyBase
  • centrosome cycle Source: FlyBase
  • cold acclimation Source: FlyBase
  • negative regulation of cell proliferation Source: FlyBase
  • negative regulation of transposition Source: FlyBase
  • NLS-bearing protein import into nucleus Source: UniProtKB
  • oogenesis Source: FlyBase
  • pole plasm mRNA localization Source: FlyBase
  • proteasome assembly Source: FlyBase
  • protein folding Source: FlyBase
  • regulation of circadian sleep/wake cycle, sleep Source: FlyBase
  • response to heat Source: FlyBase

Keywordsi

Molecular functionChaperone
Biological processStress response
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-1474151 Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation
R-DME-2029482 Regulation of actin dynamics for phagocytic cup formation
R-DME-203615 eNOS activation
R-DME-3371497 HSP90 chaperone cycle for steroid hormone receptors (SHR)
R-DME-3928662 EPHB-mediated forward signaling
R-DME-399954 Sema3A PAK dependent Axon repulsion
R-DME-4420097 VEGFA-VEGFR2 Pathway
R-DME-5218920 VEGFR2 mediated vascular permeability
R-DME-6798695 Neutrophil degranulation
R-DME-844456 The NLRP3 inflammasome
R-DME-8863795 Downregulation of ERBB2 signaling
R-DME-8937144 Aryl hydrocarbon receptor signalling
R-DME-8939211 ESR-mediated signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Heat shock protein 83
Alternative name(s):
HSP 82
Gene namesi
Name:Hsp83
Synonyms:Hsp82
ORF Names:CG1242
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0001233 Hsp83

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000629301 – 717Heat shock protein 83Add BLAST717

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei219Phosphoserine1 Publication1
Modified residuei294Phosphotyrosine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP02828
PRIDEiP02828

PTM databases

iPTMnetiP02828

Expressioni

Inductioni

In contrast to other major heat shock proteins, this one is also expressed at normal growth temperatures. It is also developmentally expressed during oogenesis.

Gene expression databases

BgeeiFBgn0001233 Expressed in 65 organ(s), highest expression level in oocyte
ExpressionAtlasiP02828 baseline and differential
GenevisibleiP02828 DM

Interactioni

Subunit structurei

Homodimer (By similarity). Forms a complex with Hop and piwi; probably Hop mediates the interaction between piwi and Hsp83 (PubMed:21186352). Interacts with shu (PubMed:22902557). Interacts with Nup358 (via TPR repeats); the interaction is required for the nuclear import of the sesquiterpenoid juvenile hormone receptor Met (PubMed:27979731).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Nelf-EP922042EBI-136814,EBI-194490

GO - Molecular functioni

Protein-protein interaction databases

BioGridi63886, 70 interactors
DIPiDIP-17366N
IntActiP02828, 5 interactors
MINTiP02828
STRINGi7227.FBpp0305095

Structurei

3D structure databases

ProteinModelPortaliP02828
SMRiP02828
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi713 – 717TPR repeat-binding5

Domaini

The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins.By similarity

Sequence similaritiesi

Belongs to the heat shock protein 90 family.Curated

Phylogenomic databases

eggNOGiKOG0020 Eukaryota
COG0326 LUCA
GeneTreeiENSGT00920000149016
HOGENOMiHOG000263872
InParanoidiP02828
KOiK04079
OMAiVKRHSEF
OrthoDBiEOG091G0270
PhylomeDBiP02828

Family and domain databases

CDDicd00075 HATPase_c, 1 hit
Gene3Di1.20.120.790, 1 hit
3.30.565.10, 1 hit
HAMAPiMF_00505 HSP90, 1 hit
InterProiView protein in InterPro
IPR003594 HATPase_C
IPR036890 HATPase_C_sf
IPR019805 Heat_shock_protein_90_CS
IPR037196 HSP90_C
IPR001404 Hsp90_fam
IPR020575 Hsp90_N
IPR020568 Ribosomal_S5_D2-typ_fold
PANTHERiPTHR11528 PTHR11528, 1 hit
PfamiView protein in Pfam
PF02518 HATPase_c, 1 hit
PF00183 HSP90, 1 hit
PIRSFiPIRSF002583 Hsp90, 1 hit
PRINTSiPR00775 HEATSHOCK90
SMARTiView protein in SMART
SM00387 HATPase_c, 1 hit
SUPFAMiSSF110942 SSF110942, 1 hit
SSF54211 SSF54211, 1 hit
SSF55874 SSF55874, 1 hit
PROSITEiView protein in PROSITE
PS00298 HSP90, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P02828-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPEEAETFAF QAEIAQLMSL IINTFYSNKE IFLRELISNA SDALDKIRYE
60 70 80 90 100
SLTDPSKLDS GKELYIKLIP NKTAGTLTII DTGIGMTKSD LVNNLGTIAK
110 120 130 140 150
SGTKAFMEAL QAGADISMIG QFGVGFYSAY LVADKVTVTS KNNDDEQYVW
160 170 180 190 200
ESSAGGSFTV RADNSEPLGR GTKIVLYIKE DQTDYLEESK IKEIVNKHSQ
210 220 230 240 250
FIGYPIKLLV EKEREKEVSD DEADDEKKEG DEKKEMETDE PKIEDVGEDE
260 270 280 290 300
DADKKDKDAK KKKTIKEKYT EDEELNKTKP IWTRNPDDIS QEEYGEFYKS
310 320 330 340 350
LTNDWEDHLA VKHFSVEGQL EFRALLFIPR RTPFDLFENQ KKRNNIKLYV
360 370 380 390 400
RRVFIMDNCE DLIPEYLNFM KGVVDSEDLP LNISREMLQQ NKVLKVIRKN
410 420 430 440 450
LVKKTMELIE ELTEDKENYK KFYDQFSKNL KLGVHEDSNN RAKLADFLRF
460 470 480 490 500
HTSASGDDFC SLADYVSRMK DNQKHVYFIT GESKDQVSNS AFVERVKARG
510 520 530 540 550
FEVVYMTEPI DEYVIQHLKE YKGKQLVSVT KEGLELPEDE SEKKKREEDK
560 570 580 590 600
AKFESLCKLM KSILDNKVEK VVVSNRLVDS PCCIVTSQFG WSANMERIMK
610 620 630 640 650
AQALRDTATM GYMAGKKQLE INPDHPIVET LRQKADADKN DKAVKDLVIL
660 670 680 690 700
LFETSLLSSG FSLDSPQVHA SRIYRMIKLG LGIDEDEPMT TDDAQSAGDA
710
PSLVEDTEDA SHMEEVD
Length:717
Mass (Da):81,865
Last modified:August 13, 1987 - v1
Checksum:iA71B96B2FE1684E6
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
M9PBL3M9PBL3_DROME
Heat shock protein 83, isoform B
Hsp83 143198_at, 4C, 83, 83K HSP, anon-EST:Liang-2.53 Hsp83 143198_at, 4C, 83, 83K HSP, anon-EST:Liang-2.53, anon-WO0068693, anon-WO0140519.209, CG1242, clone 2.53, Dmel\CG1242, DMHSP82, DmHsp83, E(sev)3A, E(sina)2, en(lz)3C, HSP82, Hsp82, hsp82, HSP83, hsp83, hsp84, HSP90, Hsp90, hsp90, HSP90A1, l(3)j5C2, ms(3)08445, ORF1, P02828, stc, Su(Raf)3A, CG1242, Dmel_CG1242
717Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti234Missing in strain: MA-10.2, DPF-13, DPF-82.1, EM-10, 178.7, 709.6 and MA-4.4. 1
Natural varianti368N → K in strain: DPF-2, DPF-30, DPF-62, MA-10.2, DPF-82.1, 178.7, DPF-13, MA-4.4, EM-10, VC-805, MA-4.2, DPF-46, DPF-77, VC-815 and 709.6. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03810 Genomic DNA Translation: CAA27435.1
X00065 Genomic DNA Translation: CAA24938.1
AE014296 Genomic DNA Translation: AAF47734.1
AY122080 mRNA Translation: AAM52592.1
U57459 Genomic DNA Translation: AAB46677.1
U57460 Genomic DNA Translation: AAB46678.1
U57461 Genomic DNA Translation: AAB46679.1
U57462 Genomic DNA Translation: AAB46680.1
U57463 Genomic DNA Translation: AAB46681.1
U57464 Genomic DNA Translation: AAB46682.1
U57465 Genomic DNA Translation: AAB46683.1
U57466 Genomic DNA Translation: AAB46684.1
U57467 Genomic DNA Translation: AAB46685.1
U57468 Genomic DNA Translation: AAB46686.1
U57469 Genomic DNA Translation: AAB46687.1
U57470 Genomic DNA Translation: AAB46688.1
U57471 Genomic DNA Translation: AAB46689.1
U57472 Genomic DNA Translation: AAB46690.1
U57473 Genomic DNA Translation: AAB46691.1
PIRiA24827 HHFF83
RefSeqiNP_001261362.1, NM_001274433.1
NP_523899.1, NM_079175.4
UniGeneiDm.2800

Genome annotation databases

EnsemblMetazoaiFBtr0073040; FBpp0072904; FBgn0001233
FBtr0332873; FBpp0305095; FBgn0001233
GeneIDi38389
KEGGidme:Dmel_CG1242

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03810 Genomic DNA Translation: CAA27435.1
X00065 Genomic DNA Translation: CAA24938.1
AE014296 Genomic DNA Translation: AAF47734.1
AY122080 mRNA Translation: AAM52592.1
U57459 Genomic DNA Translation: AAB46677.1
U57460 Genomic DNA Translation: AAB46678.1
U57461 Genomic DNA Translation: AAB46679.1
U57462 Genomic DNA Translation: AAB46680.1
U57463 Genomic DNA Translation: AAB46681.1
U57464 Genomic DNA Translation: AAB46682.1
U57465 Genomic DNA Translation: AAB46683.1
U57466 Genomic DNA Translation: AAB46684.1
U57467 Genomic DNA Translation: AAB46685.1
U57468 Genomic DNA Translation: AAB46686.1
U57469 Genomic DNA Translation: AAB46687.1
U57470 Genomic DNA Translation: AAB46688.1
U57471 Genomic DNA Translation: AAB46689.1
U57472 Genomic DNA Translation: AAB46690.1
U57473 Genomic DNA Translation: AAB46691.1
PIRiA24827 HHFF83
RefSeqiNP_001261362.1, NM_001274433.1
NP_523899.1, NM_079175.4
UniGeneiDm.2800

3D structure databases

ProteinModelPortaliP02828
SMRiP02828
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi63886, 70 interactors
DIPiDIP-17366N
IntActiP02828, 5 interactors
MINTiP02828
STRINGi7227.FBpp0305095

PTM databases

iPTMnetiP02828

Proteomic databases

PaxDbiP02828
PRIDEiP02828

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0073040; FBpp0072904; FBgn0001233
FBtr0332873; FBpp0305095; FBgn0001233
GeneIDi38389
KEGGidme:Dmel_CG1242

Organism-specific databases

CTDi38389
FlyBaseiFBgn0001233 Hsp83

Phylogenomic databases

eggNOGiKOG0020 Eukaryota
COG0326 LUCA
GeneTreeiENSGT00920000149016
HOGENOMiHOG000263872
InParanoidiP02828
KOiK04079
OMAiVKRHSEF
OrthoDBiEOG091G0270
PhylomeDBiP02828

Enzyme and pathway databases

ReactomeiR-DME-1474151 Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation
R-DME-2029482 Regulation of actin dynamics for phagocytic cup formation
R-DME-203615 eNOS activation
R-DME-3371497 HSP90 chaperone cycle for steroid hormone receptors (SHR)
R-DME-3928662 EPHB-mediated forward signaling
R-DME-399954 Sema3A PAK dependent Axon repulsion
R-DME-4420097 VEGFA-VEGFR2 Pathway
R-DME-5218920 VEGFR2 mediated vascular permeability
R-DME-6798695 Neutrophil degranulation
R-DME-844456 The NLRP3 inflammasome
R-DME-8863795 Downregulation of ERBB2 signaling
R-DME-8937144 Aryl hydrocarbon receptor signalling
R-DME-8939211 ESR-mediated signaling

Miscellaneous databases

ChiTaRSiHsp83 fly
GenomeRNAii38389
PROiPR:P02828

Gene expression databases

BgeeiFBgn0001233 Expressed in 65 organ(s), highest expression level in oocyte
ExpressionAtlasiP02828 baseline and differential
GenevisibleiP02828 DM

Family and domain databases

CDDicd00075 HATPase_c, 1 hit
Gene3Di1.20.120.790, 1 hit
3.30.565.10, 1 hit
HAMAPiMF_00505 HSP90, 1 hit
InterProiView protein in InterPro
IPR003594 HATPase_C
IPR036890 HATPase_C_sf
IPR019805 Heat_shock_protein_90_CS
IPR037196 HSP90_C
IPR001404 Hsp90_fam
IPR020575 Hsp90_N
IPR020568 Ribosomal_S5_D2-typ_fold
PANTHERiPTHR11528 PTHR11528, 1 hit
PfamiView protein in Pfam
PF02518 HATPase_c, 1 hit
PF00183 HSP90, 1 hit
PIRSFiPIRSF002583 Hsp90, 1 hit
PRINTSiPR00775 HEATSHOCK90
SMARTiView protein in SMART
SM00387 HATPase_c, 1 hit
SUPFAMiSSF110942 SSF110942, 1 hit
SSF54211 SSF54211, 1 hit
SSF55874 SSF55874, 1 hit
PROSITEiView protein in PROSITE
PS00298 HSP90, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHSP83_DROME
AccessioniPrimary (citable) accession number: P02828
Secondary accession number(s): P92174, P92202, Q9VZT3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 13, 1987
Last modified: September 12, 2018
This is version 188 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
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