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UniProtKB - P02776 (PLF4_HUMAN)
Protein
Platelet factor 4
Gene
PF4
Organism
Homo sapiens (Human)
Status
Functioni
Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.
1 PublicationGO - Molecular functioni
- chemokine activity Source: GO_Central
- CXCR3 chemokine receptor binding Source: UniProtKB
- CXCR chemokine receptor binding Source: GO_Central
- heparin binding Source: UniProtKB
GO - Biological processi
- adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: UniProtKB
- antimicrobial humoral immune response mediated by antimicrobial peptide Source: UniProtKB
- cellular response to lipopolysaccharide Source: GO_Central
- chemokine-mediated signaling pathway Source: GO_Central
- cytokine-mediated signaling pathway Source: UniProtKB
- defense response to protozoan Source: UniProtKB
- inflammatory response Source: GO_Central
- killing by host of symbiont cells Source: UniProtKB
- killing of cells of other organism Source: UniProtKB
- leukocyte chemotaxis Source: UniProtKB
- negative regulation of angiogenesis Source: UniProtKB
- negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: BHF-UCL
- negative regulation of megakaryocyte differentiation Source: UniProtKB
- negative regulation of MHC class II biosynthetic process Source: BHF-UCL
- neutrophil chemotaxis Source: GO_Central
- platelet activation Source: UniProtKB
- positive regulation of gene expression Source: BHF-UCL
- positive regulation of macrophage derived foam cell differentiation Source: BHF-UCL
- positive regulation of macrophage differentiation Source: BHF-UCL
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- positive regulation of tumor necrosis factor production Source: BHF-UCL
- regulation of cell population proliferation Source: UniProtKB
Keywordsi
Molecular function | Cytokine, Heparin-binding |
Biological process | Chemotaxis |
Enzyme and pathway databases
PathwayCommonsi | P02776 |
Reactomei | R-HSA-114608, Platelet degranulation R-HSA-140875, Common Pathway of Fibrin Clot Formation R-HSA-202733, Cell surface interactions at the vascular wall R-HSA-380108, Chemokine receptors bind chemokines R-HSA-418594, G alpha (i) signalling events R-HSA-8936459, RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function |
SignaLinki | P02776 |
Names & Taxonomyi
Protein namesi | Recommended name: Platelet factor 4Short name: PF-4 Alternative name(s): C-X-C motif chemokine 4 Iroplact Oncostatin-A Cleaved into the following chain: Alternative name(s): Endothelial cell growth inhibitor1 Publication |
Gene namesi | Name:PF4 Synonyms:CXCL4, SCYB4 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:8861, PF4 |
MIMi | 173460, gene |
neXtProti | NX_P02776 |
VEuPathDBi | HostDB:ENSG00000163737 |
Subcellular locationi
Extracellular region or secreted
- Secreted 1 Publication
Extracellular region or secreted
- extracellular region Source: Reactome
- extracellular space Source: GO_Central
Other locations
- collagen-containing extracellular matrix Source: BHF-UCL
- cytoplasm Source: UniProtKB
- platelet alpha granule lumen Source: Reactome
Keywords - Cellular componenti
SecretedPathology & Biotechi
Organism-specific databases
DisGeNETi | 5196 |
OpenTargetsi | ENSG00000163737 |
PharmGKBi | PA33203 |
Miscellaneous databases
Pharosi | P02776, Tbio |
Chemistry databases
ChEMBLi | CHEMBL3286075 |
DrugBanki | DB00055, Drotrecogin alfa DB01109, Heparin |
Genetic variation databases
BioMutai | PF4 |
DMDMi | 130304 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 31 | 5 PublicationsAdd BLAST | 31 | |
ChainiPRO_0000005068 | 32 – 101 | Platelet factor 4Add BLAST | 70 | |
ChainiPRO_0000351217 | 48 – 101 | Platelet factor 4, short form1 PublicationAdd BLAST | 54 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 41 ↔ 67 | 1 Publication | ||
Disulfide bondi | 43 ↔ 83 | 1 Publication | ||
Modified residuei | 57 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Disulfide bond, PhosphoproteinProteomic databases
jPOSTi | P02776 |
MassIVEi | P02776 |
PaxDbi | P02776 |
PeptideAtlasi | P02776 |
PRIDEi | P02776 |
ProteomicsDBi | 51593 |
TopDownProteomicsi | P02776 |
2D gel databases
OGPi | P02776 |
PTM databases
PhosphoSitePlusi | P02776 |
Expressioni
Gene expression databases
Bgeei | ENSG00000163737, Expressed in monocyte and 130 other tissues |
Genevisiblei | P02776, HS |
Organism-specific databases
HPAi | ENSG00000163737, Group enriched (bone marrow, lymphoid tissue) |
Interactioni
Subunit structurei
Binary interactionsi
P02776
Platelet factor 4 (PRO_0000005068)
With | #Exp. | IntAct |
---|---|---|
Beta-2-glycoprotein 1 (PRO_0000002059) | 4 | EBI-11809981,EBI-11809989 |
S [P0DTC2] from Severe acute respiratory syndrome coronavirus 2. | 3 | EBI-11809981,EBI-25474821 |
Platelet factor 4, short form (PRO_0000351217)
With | #Exp. | IntAct |
---|---|---|
CCL5 [P13501] | 2 | EBI-20561364,EBI-2848366 |
C-C motif chemokine 5 (PRO_0000005175) | 2 | EBI-20561364,EBI-11712923 |
GO - Molecular functioni
- chemokine activity Source: GO_Central
- CXCR3 chemokine receptor binding Source: UniProtKB
- CXCR chemokine receptor binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 111219, 28 interactors |
DIPi | DIP-56941N |
IntActi | P02776, 27 interactors |
MINTi | P02776 |
STRINGi | 9606.ENSP00000296029 |
Chemistry databases
BindingDBi | P02776 |
Miscellaneous databases
RNActi | P02776, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P02776 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P02776 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 92 – 98 | Heparin-binding | 7 |
Sequence similaritiesi
Belongs to the intercrine alpha (chemokine CxC) family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG502TF57, Eukaryota |
GeneTreei | ENSGT00940000163666 |
HOGENOMi | CLU_143902_1_2_1 |
InParanoidi | P02776 |
OMAi | CAVPQLI |
OrthoDBi | 1618797at2759 |
PhylomeDBi | P02776 |
TreeFami | TF333433 |
Family and domain databases
CDDi | cd00273, Chemokine_CXC, 1 hit |
InterProi | View protein in InterPro IPR001089, Chemokine_CXC IPR018048, Chemokine_CXC_CS IPR001811, Chemokine_IL8-like_dom IPR033899, CXC_Chemokine_domain IPR036048, Interleukin_8-like_sf IPR027222, PF4 |
PANTHERi | PTHR10179, PTHR10179, 1 hit PTHR10179:SF38, PTHR10179:SF38, 1 hit |
Pfami | View protein in Pfam PF00048, IL8, 1 hit |
PRINTSi | PR00437, SMALLCYTKCXC |
SMARTi | View protein in SMART SM00199, SCY, 1 hit |
SUPFAMi | SSF54117, SSF54117, 1 hit |
PROSITEi | View protein in PROSITE PS00471, SMALL_CYTOKINES_CXC, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P02776-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSAAGFCAS RPGLLFLGLL LLPLVVAFAS AEAEEDGDLQ CLCVKTTSQV
60 70 80 90 100
RPRHITSLEV IKAGPHCPTA QLIATLKNGR KICLDLQAPL YKKIIKKLLE
S
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 78 | N → D AA sequence (PubMed:267922).Curated | 1 | |
Sequence conflicti | 78 | N → D AA sequence (PubMed:601757).Curated | 1 |
Mass spectrometryi
Molecular mass is 6033 Da. Determined by ESI. Short form.1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M25897 mRNA Translation: AAA60066.1 AF349466 Genomic DNA Translation: AAK29643.1 CR407677 mRNA Translation: CAG28605.1 AC097709 Genomic DNA Translation: AAY41003.1 BC093965 mRNA Translation: AAH93965.1 BC112093 mRNA Translation: AAI12094.1 |
CCDSi | CCDS3562.1 |
PIRi | A60161, PFHU4 |
RefSeqi | NP_002610.1, NM_002619.3 |
Genome annotation databases
Ensembli | ENST00000296029; ENSP00000296029; ENSG00000163737 |
GeneIDi | 5196 |
KEGGi | hsa:5196 |
MANE-Selecti | ENST00000296029.4; ENSP00000296029.3; NM_002619.4; NP_002610.1 |
UCSCi | uc003hhi.4, human |
Similar proteinsi
Cross-referencesi
Web resourcesi
Wikipedia CXCL4 entry |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M25897 mRNA Translation: AAA60066.1 AF349466 Genomic DNA Translation: AAK29643.1 CR407677 mRNA Translation: CAG28605.1 AC097709 Genomic DNA Translation: AAY41003.1 BC093965 mRNA Translation: AAH93965.1 BC112093 mRNA Translation: AAI12094.1 |
CCDSi | CCDS3562.1 |
PIRi | A60161, PFHU4 |
RefSeqi | NP_002610.1, NM_002619.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1DN3 | NMR | - | A | 87-101 | [»] | |
1F9Q | X-ray | 2.00 | A/B/C/D | 32-101 | [»] | |
1F9R | X-ray | 2.00 | A/B/C/D | 32-101 | [»] | |
1F9S | X-ray | 2.38 | A/B/C/D | 32-101 | [»] | |
1PFM | NMR | - | A/B/C/D | 39-101 | [»] | |
1PFN | NMR | - | A/B/C/D | 39-101 | [»] | |
1RHP | X-ray | 2.40 | A/B/C/D | 32-101 | [»] | |
4R9W | X-ray | 2.50 | A/B | 32-101 | [»] | |
4R9Y | X-ray | 4.11 | A/B/C/D | 32-101 | [»] | |
4RAU | X-ray | 3.74 | C/F/I/L/O/R/U/X | 32-101 | [»] | |
SMRi | P02776 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111219, 28 interactors |
DIPi | DIP-56941N |
IntActi | P02776, 27 interactors |
MINTi | P02776 |
STRINGi | 9606.ENSP00000296029 |
Chemistry databases
BindingDBi | P02776 |
ChEMBLi | CHEMBL3286075 |
DrugBanki | DB00055, Drotrecogin alfa DB01109, Heparin |
PTM databases
PhosphoSitePlusi | P02776 |
Genetic variation databases
BioMutai | PF4 |
DMDMi | 130304 |
2D gel databases
OGPi | P02776 |
Proteomic databases
jPOSTi | P02776 |
MassIVEi | P02776 |
PaxDbi | P02776 |
PeptideAtlasi | P02776 |
PRIDEi | P02776 |
ProteomicsDBi | 51593 |
TopDownProteomicsi | P02776 |
Protocols and materials databases
ABCDi | P02776, 2 sequenced antibodies |
Antibodypediai | 24586, 507 antibodies from 32 providers |
DNASUi | 5196 |
Genome annotation databases
Ensembli | ENST00000296029; ENSP00000296029; ENSG00000163737 |
GeneIDi | 5196 |
KEGGi | hsa:5196 |
MANE-Selecti | ENST00000296029.4; ENSP00000296029.3; NM_002619.4; NP_002610.1 |
UCSCi | uc003hhi.4, human |
Organism-specific databases
CTDi | 5196 |
DisGeNETi | 5196 |
GeneCardsi | PF4 |
HGNCi | HGNC:8861, PF4 |
HPAi | ENSG00000163737, Group enriched (bone marrow, lymphoid tissue) |
MIMi | 173460, gene |
neXtProti | NX_P02776 |
OpenTargetsi | ENSG00000163737 |
PharmGKBi | PA33203 |
VEuPathDBi | HostDB:ENSG00000163737 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502TF57, Eukaryota |
GeneTreei | ENSGT00940000163666 |
HOGENOMi | CLU_143902_1_2_1 |
InParanoidi | P02776 |
OMAi | CAVPQLI |
OrthoDBi | 1618797at2759 |
PhylomeDBi | P02776 |
TreeFami | TF333433 |
Enzyme and pathway databases
PathwayCommonsi | P02776 |
Reactomei | R-HSA-114608, Platelet degranulation R-HSA-140875, Common Pathway of Fibrin Clot Formation R-HSA-202733, Cell surface interactions at the vascular wall R-HSA-380108, Chemokine receptors bind chemokines R-HSA-418594, G alpha (i) signalling events R-HSA-8936459, RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function |
SignaLinki | P02776 |
Miscellaneous databases
BioGRID-ORCSi | 5196, 15 hits in 988 CRISPR screens |
EvolutionaryTracei | P02776 |
GeneWikii | Platelet_factor_4 |
GenomeRNAii | 5196 |
Pharosi | P02776, Tbio |
PROi | PR:P02776 |
RNActi | P02776, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000163737, Expressed in monocyte and 130 other tissues |
Genevisiblei | P02776, HS |
Family and domain databases
CDDi | cd00273, Chemokine_CXC, 1 hit |
InterProi | View protein in InterPro IPR001089, Chemokine_CXC IPR018048, Chemokine_CXC_CS IPR001811, Chemokine_IL8-like_dom IPR033899, CXC_Chemokine_domain IPR036048, Interleukin_8-like_sf IPR027222, PF4 |
PANTHERi | PTHR10179, PTHR10179, 1 hit PTHR10179:SF38, PTHR10179:SF38, 1 hit |
Pfami | View protein in Pfam PF00048, IL8, 1 hit |
PRINTSi | PR00437, SMALLCYTKCXC |
SMARTi | View protein in SMART SM00199, SCY, 1 hit |
SUPFAMi | SSF54117, SSF54117, 1 hit |
PROSITEi | View protein in PROSITE PS00471, SMALL_CYTOKINES_CXC, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PLF4_HUMAN | |
Accessioni | P02776Primary (citable) accession number: P02776 Secondary accession number(s): Q53X61, Q9UC64, Q9UC65 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 1, 1989 | |
Last modified: | February 23, 2022 | |
This is version 202 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 4
Human chromosome 4: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families