UniProtKB - P02722 (ADT1_BOVIN)
ADP/ATP translocase 1
SLC25A4
Functioni
ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to fuel the cell (By similarity).
Cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state): operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic (c-state) or matrix (m-state) side of the inner mitochondrial membrane (By similarity).
In addition to its ADP:ATP antiporter activity, also involved in mitochondrial uncoupling and mitochondrial permeability transition pore (mPTP) activity (By similarity).
Plays a role in mitochondrial uncoupling by acting as a proton transporter: proton transport uncouples the proton flows via the electron transport chain and ATP synthase to reduce the efficiency of ATP production and cause mitochondrial thermogenesis (PubMed:7961643).
Proton transporter activity is inhibited by ADP:ATP antiporter activity, suggesting that SLC25A4/ANT1 acts as a master regulator of mitochondrial energy output by maintaining a delicate balance between ATP production (ADP:ATP antiporter activity) and thermogenesis (proton transporter activity) (By similarity).
Proton transporter activity requires free fatty acids as cofactor, but does not transport it (PubMed:7961643).
Probably mediates mitochondrial uncoupling in tissues that do not express UCP1 (By similarity).
Also plays a key role in mPTP opening, a non-specific pore that enables free passage of the mitochondrial membranes to solutes of up to 1.5 kDa, and which contributes to cell death (By similarity).
It is however unclear if SLC25A4/ANT1 constitutes a pore-forming component of mPTP or regulates it (By similarity).
Acts as a regulator of mitophagy independently of ADP:ATP antiporter activity: promotes mitophagy via interaction with TIMM44, leading to inhibit the presequence translocase TIMM23, thereby promoting stabilization of PINK1 (By similarity).
By similarity1 PublicationCatalytic activityi
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 80 | ADP1 Publication | 1 | |
Binding sitei | 92 | ADP1 Publication | 1 | |
Binding sitei | 235 | ADP1 Publication | 1 |
GO - Molecular functioni
- ATP:ADP antiporter activity Source: UniProtKB
- oxidative phosphorylation uncoupler activity Source: UniProtKB
GO - Biological processi
- adaptive thermogenesis Source: UniProtKB
- ADP transport Source: UniProtKB
- mitochondrial ADP transmembrane transport Source: UniProtKB
- mitochondrial ATP transmembrane transport Source: UniProtKB
- positive regulation of mitophagy Source: UniProtKB
- regulation of mitochondrial membrane permeability Source: UniProtKB
Keywordsi
Biological process | Antiport, Transport |
Enzyme and pathway databases
Reactomei | R-BTA-83936, Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane |
Names & Taxonomyi
Protein namesi | Recommended name: ADP/ATP translocase 11 PublicationAlternative name(s): ADP,ATP carrier protein 11 Publication ADP,ATP carrier protein, heart isoform T11 Publication Adenine nucleotide translocator 11 Publication Short name: ANT 11 Publication Solute carrier family 25 member 4Curated |
Gene namesi | |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | HostDB:ENSBTAG00000013208 |
VGNCi | VGNC:34765, SLC25A4 |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane 2 Publications; Multi-pass membrane protein 2 Publications
Other locations
- Membrane By similarity; Multi-pass membrane protein 2 Publications
Note: The complex formed with ARL2BP, ARL2 and SLC25A4/ANT1 is expressed in mitochondria (By similarity). May localize to non-mitochondrial membranes (By similarity).By similarity
Mitochondrion
- integral component of mitochondrial membrane Source: UniProtKB
- mitochondrial inner membrane Source: UniProtKB
- mitochondrial permeability transition pore complex Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 7 | Mitochondrial intermembraneCurated | 7 | |
Transmembranei | 8 – 37 | Helical; Name=12 PublicationsAdd BLAST | 30 | |
Topological domaini | 38 – 74 | Mitochondrial matrixCuratedAdd BLAST | 37 | |
Transmembranei | 75 – 99 | Helical; Name=22 PublicationsAdd BLAST | 25 | |
Topological domaini | 100 – 109 | Mitochondrial intermembraneCurated | 10 | |
Transmembranei | 110 – 130 | Helical; Name=32 PublicationsAdd BLAST | 21 | |
Topological domaini | 131 – 178 | Mitochondrial matrixCuratedAdd BLAST | 48 | |
Transmembranei | 179 – 199 | Helical; Name=42 PublicationsAdd BLAST | 21 | |
Topological domaini | 200 – 210 | Mitochondrial intermembraneCuratedAdd BLAST | 11 | |
Transmembranei | 211 – 231 | Helical; Name=52 PublicationsAdd BLAST | 21 | |
Topological domaini | 232 – 273 | Mitochondrial matrixCuratedAdd BLAST | 42 | |
Transmembranei | 274 – 291 | Helical; Name=62 PublicationsAdd BLAST | 18 | |
Topological domaini | 292 – 298 | Mitochondrial intermembraneCurated | 7 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000090573 | 2 – 298 | ADP/ATP translocase 1Add BLAST | 297 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserine1 Publication | 1 | |
Modified residuei | 7 | PhosphoserineBy similarity | 1 | |
Modified residuei | 52 | N6,N6,N6-trimethyllysineBy similarity | 1 | |
Modified residuei | 52 | N6-methyllysineSequence analysis | 1 | |
Modified residuei | 147 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 160 | S-nitrosocysteineBy similarity | 1 | |
Modified residuei | 245 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 272 | N6-succinyllysineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Methylation, Phosphoprotein, S-nitrosylationProteomic databases
PaxDbi | P02722 |
PeptideAtlasi | P02722 |
PRIDEi | P02722 |
PTM databases
iPTMneti | P02722 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSBTAG00000013208, Expressed in heart and 97 other tissues |
Interactioni
Subunit structurei
Monomer (PubMed:16226253).
Found in a complex with ARL2, ARL2BP and SLC25A4/ANT1.
Interacts with ARL2BP.
Interacts with TIMM44; leading to inhibit the presequence translocase TIMM23, thereby promoting stabilization of PINK1 (By similarity).
By similarity1 PublicationProtein-protein interaction databases
IntActi | P02722, 1 interactor |
STRINGi | 9913.ENSBTAP00000017580 |
Structurei
Secondary structure
3D structure databases
SMRi | P02722 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P02722 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 6 – 98 | Solcar 1Add BLAST | 93 | |
Repeati | 111 – 201 | Solcar 2Add BLAST | 91 | |
Repeati | 212 – 297 | Solcar 3Add BLAST | 86 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 235 – 240 | Important for transport activityBy similarity | 6 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 235 – 240 | Nucleotide carrier signature motifCurated | 6 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0749, Eukaryota |
GeneTreei | ENSGT00940000154622 |
HOGENOMi | CLU_015166_12_0_1 |
InParanoidi | P02722 |
OMAi | YDGIVEC |
OrthoDBi | 870903at2759 |
TreeFami | TF300743 |
Family and domain databases
Gene3Di | 1.50.40.10, 1 hit |
InterProi | View protein in InterPro IPR002113, ADT_euk_type IPR002067, Mit_carrier IPR018108, Mitochondrial_sb/sol_carrier IPR023395, Mt_carrier_dom_sf |
PANTHERi | PTHR45635, PTHR45635, 1 hit |
Pfami | View protein in Pfam PF00153, Mito_carr, 3 hits |
PRINTSi | PR00927, ADPTRNSLCASE PR00926, MITOCARRIER |
SUPFAMi | SSF103506, SSF103506, 1 hit |
PROSITEi | View protein in PROSITE PS50920, SOLCAR, 3 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MSDQALSFLK DFLAGGVAAA ISKTAVAPIE RVKLLLQVQH ASKQISAEKQ
60 70 80 90 100
YKGIIDCVVR IPKEQGFLSF WRGNLANVIR YFPTQALNFA FKDKYKQIFL
110 120 130 140 150
GGVDRHKQFW RYFAGNLASG GAAGATSLCF VYPLDFARTR LAADVGKGAA
160 170 180 190 200
QREFTGLGNC ITKIFKSDGL RGLYQGFNVS VQGIIIYRAA YFGVYDTAKG
210 220 230 240 250
MLPDPKNVHI IVSWMIAQTV TAVAGLVSYP FDTVRRRMMM QSGRKGADIM
260 270 280 290
YTGTVDCWRK IAKDEGPKAF FKGAWSNVLR GMGGAFVLVL YDEIKKFV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M13783 mRNA Translation: AAA30363.1 M24102 mRNA Translation: AAA30768.1 |
PIRi | A43646, XWBO |
RefSeqi | NP_777083.1, NM_174658.2 |
Genome annotation databases
Ensembli | ENSBTAT00000017580; ENSBTAP00000017580; ENSBTAG00000013208 |
GeneIDi | 282478 |
KEGGi | bta:282478 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M13783 mRNA Translation: AAA30363.1 M24102 mRNA Translation: AAA30768.1 |
PIRi | A43646, XWBO |
RefSeqi | NP_777083.1, NM_174658.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1OKC | X-ray | 2.20 | A | 2-298 | [»] | |
2C3E | X-ray | 2.80 | A | 2-298 | [»] | |
SMRi | P02722 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P02722, 1 interactor |
STRINGi | 9913.ENSBTAP00000017580 |
PTM databases
iPTMneti | P02722 |
Proteomic databases
PaxDbi | P02722 |
PeptideAtlasi | P02722 |
PRIDEi | P02722 |
Genome annotation databases
Ensembli | ENSBTAT00000017580; ENSBTAP00000017580; ENSBTAG00000013208 |
GeneIDi | 282478 |
KEGGi | bta:282478 |
Organism-specific databases
CTDi | 291 |
VEuPathDBi | HostDB:ENSBTAG00000013208 |
VGNCi | VGNC:34765, SLC25A4 |
Phylogenomic databases
eggNOGi | KOG0749, Eukaryota |
GeneTreei | ENSGT00940000154622 |
HOGENOMi | CLU_015166_12_0_1 |
InParanoidi | P02722 |
OMAi | YDGIVEC |
OrthoDBi | 870903at2759 |
TreeFami | TF300743 |
Enzyme and pathway databases
Reactomei | R-BTA-83936, Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane |
Miscellaneous databases
EvolutionaryTracei | P02722 |
Gene expression databases
Bgeei | ENSBTAG00000013208, Expressed in heart and 97 other tissues |
Family and domain databases
Gene3Di | 1.50.40.10, 1 hit |
InterProi | View protein in InterPro IPR002113, ADT_euk_type IPR002067, Mit_carrier IPR018108, Mitochondrial_sb/sol_carrier IPR023395, Mt_carrier_dom_sf |
PANTHERi | PTHR45635, PTHR45635, 1 hit |
Pfami | View protein in Pfam PF00153, Mito_carr, 3 hits |
PRINTSi | PR00927, ADPTRNSLCASE PR00926, MITOCARRIER |
SUPFAMi | SSF103506, SSF103506, 1 hit |
PROSITEi | View protein in PROSITE PS50920, SOLCAR, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | ADT1_BOVIN | |
Accessioni | P02722Primary (citable) accession number: P02722 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 172 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families