UniProtKB - P02698 (GBG1_BOVIN)
Protein
Guanine nucleotide-binding protein G(T) subunit gamma-T1
Gene
GNGT1
Organism
Bos taurus (Bovine)
Status
Functioni
Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
Caution
In PubMed:3917402 the authors propose that Cys-36 and Cys-37 are disulfide bonded because they could not be observed during peptide sequencing, but were observed after reduction by dithiothreitol and reaction with labeled iodoacetamide. The crystallographic structures do not support these cysteines being disulfide bonded. Artifactual oxidation and some other cysteine modifications might be consistent with these observations.Curated
GO - Molecular functioni
- G-protein beta-subunit binding Source: GO_Central
GO - Biological processi
- G protein-coupled receptor signaling pathway Source: GO_Central
Keywordsi
Molecular function | Transducer |
Enzyme and pathway databases
Reactomei | R-BTA-1296041, Activation of G protein gated Potassium channels R-BTA-202040, G-protein activation R-BTA-2485179, Activation of the phototransduction cascade R-BTA-381676, Glucagon-like Peptide-1 (GLP1) regulates insulin secretion R-BTA-381753, Olfactory Signaling Pathway R-BTA-392170, ADP signalling through P2Y purinoceptor 12 R-BTA-392451, G beta:gamma signalling through PI3Kgamma R-BTA-400042, Adrenaline,noradrenaline inhibits insulin secretion R-BTA-4086398, Ca2+ pathway R-BTA-416476, G alpha (q) signalling events R-BTA-416482, G alpha (12/13) signalling events R-BTA-418217, G beta:gamma signalling through PLC beta R-BTA-418555, G alpha (s) signalling events R-BTA-418592, ADP signalling through P2Y purinoceptor 1 R-BTA-418594, G alpha (i) signalling events R-BTA-418597, G alpha (z) signalling events R-BTA-420092, Glucagon-type ligand receptors R-BTA-428930, Thromboxane signalling through TP receptor R-BTA-432040, Vasopressin regulates renal water homeostasis via Aquaporins R-BTA-456926, Thrombin signalling through proteinase activated receptors (PARs) R-BTA-500657, Presynaptic function of Kainate receptors R-BTA-6814122, Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding R-BTA-8964315, G beta:gamma signalling through BTK R-BTA-8964616, G beta:gamma signalling through CDC42 R-BTA-9009391, Extra-nuclear estrogen signaling R-BTA-997272, Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits |
Names & Taxonomyi
Protein namesi | Recommended name: Guanine nucleotide-binding protein G(T) subunit gamma-T1Alternative name(s): Transducin gamma chain |
Gene namesi | Name:GNGT1 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VGNCi | VGNC:29470, GNGT1 |
Subcellular locationi
Plasma membrane
- Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated
Plasma Membrane
- heterotrimeric G-protein complex Source: GO_Central
Other locations
- G-protein beta/gamma-subunit complex Source: GO_Central
- photoreceptor disc membrane Source: Reactome
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000012601 | 2 – 71 | Guanine nucleotide-binding protein G(T) subunit gamma-T1Add BLAST | 70 | |
PropeptideiPRO_0000012602 | 72 – 74 | Removed in mature form | 3 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 71 | Cysteine methyl ester1 Publication | 1 | |
Lipidationi | 71 | S-farnesyl cysteine3 Publications | 1 |
Keywords - PTMi
Lipoprotein, Methylation, PrenylationProteomic databases
PaxDbi | P02698 |
PRIDEi | P02698 |
Expressioni
Tissue specificityi
Retinal rod outer segment.
Gene expression databases
Bgeei | ENSBTAG00000002674, Expressed in spermatocyte and 9 other tissues |
ExpressionAtlasi | P02698, baseline |
Interactioni
Subunit structurei
G proteins are composed of 3 units, alpha, beta and gamma.
GO - Molecular functioni
- G-protein beta-subunit binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 159110, 1 interactor |
CORUMi | P02698 |
DIPi | DIP-29228N |
IntActi | P02698, 1 interactor |
MINTi | P02698 |
STRINGi | 9913.ENSBTAP00000003465 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P02698 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P02698 |
Family & Domainsi
Sequence similaritiesi
Belongs to the G protein gamma family.Curated
Phylogenomic databases
eggNOGi | KOG4119, Eukaryota |
GeneTreei | ENSGT01000000214376 |
HOGENOMi | CLU_168377_2_0_1 |
InParanoidi | P02698 |
OMAi | CEEFRDY |
OrthoDBi | 1573820at2759 |
TreeFami | TF319909 |
Family and domain databases
CDDi | cd00068, GGL, 1 hit |
Gene3Di | 4.10.260.10, 1 hit |
InterProi | View protein in InterPro IPR015898, G-protein_gamma-like_dom IPR036284, GGL_sf IPR001770, Gprotein-gamma |
PANTHERi | PTHR13809, PTHR13809, 1 hit |
Pfami | View protein in Pfam PF00631, G-gamma, 1 hit |
PRINTSi | PR00321, GPROTEING |
SMARTi | View protein in SMART SM00224, GGL, 1 hit |
SUPFAMi | SSF48670, SSF48670, 1 hit |
PROSITEi | View protein in PROSITE PS50058, G_PROTEIN_GAMMA, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P02698-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPVINIEDLT EKDKLKMEVD QLKKEVTLER MLVSKCCEEF RDYVEERSGE
60 70
DPLVKGIPED KNPFKELKGG CVIS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A3Q1M017 | A0A3Q1M017_BOVIN | Guanine nucleotide-binding protein ... | GNGT1 | 79 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K03255 mRNA Translation: AAA30794.1 K02199 mRNA Translation: AAA30793.1 S62031, S62029 Genomic DNA Translation: AAB26895.1 K02436 mRNA Translation: AAA30788.1 |
PIRi | I46939, RGBOGT |
RefSeqi | NP_776752.1, NM_174327.3 XP_010802285.1, XM_010803983.2 |
Genome annotation databases
Ensembli | ENSBTAT00000003465; ENSBTAP00000003465; ENSBTAG00000002674 ENSBTAT00000082378; ENSBTAP00000060879; ENSBTAG00000002674 |
GeneIDi | 281796 |
KEGGi | bta:281796 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K03255 mRNA Translation: AAA30794.1 K02199 mRNA Translation: AAA30793.1 S62031, S62029 Genomic DNA Translation: AAB26895.1 K02436 mRNA Translation: AAA30788.1 |
PIRi | I46939, RGBOGT |
RefSeqi | NP_776752.1, NM_174327.3 XP_010802285.1, XM_010803983.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1A0R | X-ray | 2.80 | G | 2-66 | [»] | |
1B9X | X-ray | 3.00 | B | 1-68 | [»] | |
1B9Y | X-ray | 3.00 | B | 1-68 | [»] | |
1GOT | X-ray | 2.00 | G | 2-66 | [»] | |
1MF6 | NMR | - | A | 60-71 | [»] | |
1TBG | X-ray | 2.10 | E/F/G/H | 2-68 | [»] | |
2TRC | X-ray | 2.40 | G | 1-68 | [»] | |
5KDO | X-ray | 1.90 | G | 1-74 | [»] | |
6B20 | X-ray | 2.34 | C/D | 7-66 | [»] | |
6OY9 | electron microscopy | 3.90 | G | 2-74 | [»] | |
6OYA | electron microscopy | 3.30 | G | 2-74 | [»] | |
6QNO | electron microscopy | 4.38 | G | 1-74 | [»] | |
SMRi | P02698 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 159110, 1 interactor |
CORUMi | P02698 |
DIPi | DIP-29228N |
IntActi | P02698, 1 interactor |
MINTi | P02698 |
STRINGi | 9913.ENSBTAP00000003465 |
Proteomic databases
PaxDbi | P02698 |
PRIDEi | P02698 |
Protocols and materials databases
ABCDi | P02698, 3 sequenced antibodies |
Genome annotation databases
Ensembli | ENSBTAT00000003465; ENSBTAP00000003465; ENSBTAG00000002674 ENSBTAT00000082378; ENSBTAP00000060879; ENSBTAG00000002674 |
GeneIDi | 281796 |
KEGGi | bta:281796 |
Organism-specific databases
CTDi | 2792 |
VGNCi | VGNC:29470, GNGT1 |
Phylogenomic databases
eggNOGi | KOG4119, Eukaryota |
GeneTreei | ENSGT01000000214376 |
HOGENOMi | CLU_168377_2_0_1 |
InParanoidi | P02698 |
OMAi | CEEFRDY |
OrthoDBi | 1573820at2759 |
TreeFami | TF319909 |
Enzyme and pathway databases
Reactomei | R-BTA-1296041, Activation of G protein gated Potassium channels R-BTA-202040, G-protein activation R-BTA-2485179, Activation of the phototransduction cascade R-BTA-381676, Glucagon-like Peptide-1 (GLP1) regulates insulin secretion R-BTA-381753, Olfactory Signaling Pathway R-BTA-392170, ADP signalling through P2Y purinoceptor 12 R-BTA-392451, G beta:gamma signalling through PI3Kgamma R-BTA-400042, Adrenaline,noradrenaline inhibits insulin secretion R-BTA-4086398, Ca2+ pathway R-BTA-416476, G alpha (q) signalling events R-BTA-416482, G alpha (12/13) signalling events R-BTA-418217, G beta:gamma signalling through PLC beta R-BTA-418555, G alpha (s) signalling events R-BTA-418592, ADP signalling through P2Y purinoceptor 1 R-BTA-418594, G alpha (i) signalling events R-BTA-418597, G alpha (z) signalling events R-BTA-420092, Glucagon-type ligand receptors R-BTA-428930, Thromboxane signalling through TP receptor R-BTA-432040, Vasopressin regulates renal water homeostasis via Aquaporins R-BTA-456926, Thrombin signalling through proteinase activated receptors (PARs) R-BTA-500657, Presynaptic function of Kainate receptors R-BTA-6814122, Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding R-BTA-8964315, G beta:gamma signalling through BTK R-BTA-8964616, G beta:gamma signalling through CDC42 R-BTA-9009391, Extra-nuclear estrogen signaling R-BTA-997272, Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits |
Miscellaneous databases
EvolutionaryTracei | P02698 |
Gene expression databases
Bgeei | ENSBTAG00000002674, Expressed in spermatocyte and 9 other tissues |
ExpressionAtlasi | P02698, baseline |
Family and domain databases
CDDi | cd00068, GGL, 1 hit |
Gene3Di | 4.10.260.10, 1 hit |
InterProi | View protein in InterPro IPR015898, G-protein_gamma-like_dom IPR036284, GGL_sf IPR001770, Gprotein-gamma |
PANTHERi | PTHR13809, PTHR13809, 1 hit |
Pfami | View protein in Pfam PF00631, G-gamma, 1 hit |
PRINTSi | PR00321, GPROTEING |
SMARTi | View protein in SMART SM00224, GGL, 1 hit |
SUPFAMi | SSF48670, SSF48670, 1 hit |
PROSITEi | View protein in PROSITE PS50058, G_PROTEIN_GAMMA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GBG1_BOVIN | |
Accessioni | P02698Primary (citable) accession number: P02698 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 176 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families