Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Myelin basic protein

Gene

MBP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.1 Publication

GO - Molecular functioni

  • calmodulin binding Source: CAFA
  • protease binding Source: Ensembl
  • structural constituent of myelin sheath Source: GO_Central

GO - Biological processi

  • axon ensheathment Source: ProtInc
  • central nervous system development Source: ProtInc
  • chemical synaptic transmission Source: ProtInc
  • immune response Source: ProtInc
  • maintenance of permeability of blood-brain barrier Source: CAFA
  • MAPK cascade Source: CAFA
  • membrane organization Source: Ensembl
  • myelination Source: GO_Central
  • negative regulation of heterotypic cell-cell adhesion Source: CAFA
  • positive regulation of chemokine (C-C motif) ligand 2 secretion Source: CAFA
  • positive regulation of interleukin-6 secretion Source: CAFA
  • positive regulation of metalloendopeptidase activity Source: CAFA
  • response to toxic substance Source: Ensembl
  • sensory perception of sound Source: Ensembl
  • substantia nigra development Source: UniProtKB

Enzyme and pathway databases

SIGNORiP02686

Names & Taxonomyi

Protein namesi
Recommended name:
Myelin basic protein
Short name:
MBP
Alternative name(s):
Myelin A1 protein
Myelin membrane encephalitogenic protein
Gene namesi
Name:MBP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

EuPathDBiHostDB:ENSG00000197971.14
HGNCiHGNC:6925 MBP
MIMi159430 gene
neXtProtiNX_P02686

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

Pathology & Biotechi

Involvement in diseasei

The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).

Keywords - Diseasei

Autoimmune encephalomyelitis

Organism-specific databases

DisGeNETi4155
OpenTargetsiENSG00000197971
PharmGKBiPA30667

Polymorphism and mutation databases

BioMutaiMBP

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001589901 – 304Myelin basic proteinAdd BLAST304
Isoform 3 (identifier: P02686-3)
Initiator methionineiRemoved1 Publication
Isoform 4 (identifier: P02686-4)
Initiator methionineiRemoved1 Publication
Isoform 5 (identifier: P02686-5)
Initiator methionineiRemoved1 Publication
Isoform 6 (identifier: P02686-6)
Initiator methionineiRemoved1 Publication

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei96PhosphoserineCombined sources1
Modified residuei141PhosphoserineBy similarity1
Modified residuei146PhosphoserineBy similarity1
Modified residuei148PhosphotyrosineBy similarity1
Modified residuei151PhosphothreonineBy similarity1
Modified residuei153PhosphoserineBy similarity1
Modified residuei154PhosphothreonineBy similarity1
Modified residuei159Citrulline; in form C81 Publication1
Modified residuei165Citrulline; in form C81 Publication1
Modified residuei167Citrulline1 Publication1
Modified residuei169PhosphothreonineBy similarity1
Modified residuei174PhosphoserineBy similarity1
Modified residuei177Omega-N-methylarginineBy similarity1
Modified residuei183Omega-N-methylarginineBy similarity1
Modified residuei190PhosphoserineBy similarity1
Modified residuei199Citrulline1 Publication1
Modified residuei203PhosphotyrosineBy similarity1
Modified residuei210PhosphoserineBy similarity1
Modified residuei214PhosphothreonineBy similarity1
Modified residuei229PhosphothreonineBy similarity1
Modified residuei231Citrulline1 Publication1
Modified residuei232PhosphothreonineBy similarity1
Modified residuei237Deamidated glutamineBy similarity1
Modified residuei241Omega-N-methylarginine; alternate1 Publication1
Modified residuei241Symmetric dimethylarginine; alternate1 Publication1
Modified residuei249PhosphoserineBy similarity1
Modified residuei256Citrulline; in form C81 Publication1
Modified residuei264Citrulline; in form C82 Publications1
Modified residuei281Deamidated glutamineBy similarity1
Modified residuei293Citrulline; in form C81 Publication1
Modified residuei295PhosphoserineBy similarity1
Modified residuei296Citrulline1 Publication1
Modified residuei299Phosphoserine; by UHMK11 Publication1
Modified residuei303Citrulline1 Publication1
Modified residuei304Citrulline; in form C81 Publication1
Isoform 3 (identifier: P02686-3)
Modified residuei2N-acetylalanine1 Publication1
Isoform 4 (identifier: P02686-4)
Modified residuei2N-acetylalanine1 Publication1
Isoform 5 (identifier: P02686-5)
Modified residuei2N-acetylalanine1 Publication1
Isoform 6 (identifier: P02686-6)
Modified residuei2N-acetylalanine1 Publication1

Post-translational modificationi

Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.3 Publications
The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
Phosphorylated by TAOK2, VRK2, MAPK11, MAPK12, MAPK14 and MINK1.

Keywords - PTMi

Acetylation, Citrullination, Methylation, Phosphoprotein

Proteomic databases

EPDiP02686
MaxQBiP02686
PeptideAtlasiP02686
PRIDEiP02686
ProteomicsDBi51547
51548 [P02686-2]
51549 [P02686-3]
51550 [P02686-4]
51551 [P02686-5]
51552 [P02686-6]

PTM databases

iPTMnetiP02686
PhosphoSitePlusiP02686
SwissPalmiP02686

Miscellaneous databases

PMAP-CutDBiA8MYL4

Expressioni

Tissue specificityi

MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.1 Publication

Developmental stagei

Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.

Gene expression databases

BgeeiENSG00000197971
CleanExiHS_MBP
ExpressionAtlasiP02686 baseline and differential
GenevisibleiP02686 HS

Organism-specific databases

HPAiCAB002300
HPA049222
HPA064368

Interactioni

Subunit structurei

Homodimer. Isoform 3 exists as a homodimer.3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • calmodulin binding Source: CAFA
  • protease binding Source: Ensembl

Protein-protein interaction databases

BioGridi110325, 88 interactors
DIPiDIP-36624N
IntActiP02686, 19 interactors
MINTiP02686

Structurei

3D structure databases

DisProtiDP00236
ProteinModelPortaliP02686
SMRiP02686
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02686

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni179 – 222Induces experimental autoimmune encephalomyelitis (EAE) 1Add BLAST44
Regioni246 – 256Induces experimental autoimmune encephalomyelitis (EAE) 2Add BLAST11

Sequence similaritiesi

Belongs to the myelin basic protein family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000014772
HOVERGENiHBG008347
InParanoidiP02686
KOiK17269
OMAiLMASQKR
OrthoDBiEOG091G11QQ
PhylomeDBiP02686
TreeFamiTF333391

Family and domain databases

InterProiView protein in InterPro
IPR000548 Myelin_BP
PANTHERiPTHR11429 PTHR11429, 1 hit
PfamiView protein in Pfam
PF01669 Myelin_MBP, 1 hit
PRINTSiPR00212 MYELINMBP
PROSITEiView protein in PROSITE
PS00569 MYELIN_MBP, 1 hit

Sequences (6)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: P02686-1) [UniParc]FASTAAdd to basket
Also known as: Golli-MBP1, HOG7

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGNHAGKREL NAEKASTNSE TNRGESEKKR NLGELSRTTS EDNEVFGEAD
60 70 80 90 100
ANQNNGTSSQ DTAVTDSKRT ADPKNAWQDA HPADPGSRPH LIRLFSRDAP
110 120 130 140 150
GREDNTFKDR PSESDELQTI QEDSAATSES LDVMASQKRP SQRHGSKYLA
160 170 180 190 200
TASTMDHARH GFLPRHRDTG ILDSIGRFFG GDRGAPKRGS GKDSHHPART
210 220 230 240 250
AHYGSLPQKS HGRTQDENPV VHFFKNIVTP RTPPPSQGKG RGLSLSRFSW
260 270 280 290 300
GAEGQRPGFG YGGRASDYKS AHKGFKGVDA QGTLSKIFKL GGRDSRSGSP

MARR
Length:304
Mass (Da):33,117
Last modified:October 18, 2001 - v3
Checksum:i4AD7305C1D5434C4
GO
Isoform 2 (identifier: P02686-2) [UniParc]FASTAAdd to basket
Also known as: Golli-MBP2, HOG5

The sequence of this isoform differs from the canonical sequence as follows:
     193-304: DSHHPARTAH...SRSGSPMARR → VSSEE

Show »
Length:197
Mass (Da):21,522
Checksum:i0B2F47BA214706F2
GO
Isoform 3 (identifier: P02686-3) [UniParc]FASTAAdd to basket
Also known as: MBP1, 21.5 kDa

The sequence of this isoform differs from the canonical sequence as follows:
     1-133: Missing.
     192-192: K → KVPWLKPGRSPLPSHARSQPGLCNMYK

Note: Contains a non-traditional PY nuclear localization signal. Mutagenesis of Cys-81 to Ser prevents dimerization.1 Publication
Show »
Length:197
Mass (Da):21,493
Checksum:i230FD99ECEC75FC5
GO
Isoform 4 (identifier: P02686-4) [UniParc]FASTAAdd to basket
Also known as: MBP2, 20.2 kDa

The sequence of this isoform differs from the canonical sequence as follows:
     1-133: Missing.
     192-192: K → KVPWLKPGRSPLPSHARSQPGLCNMYK
     240-250: Missing.

Show »
Length:186
Mass (Da):20,246
Checksum:i6CCF96DD90FE1CEB
GO
Isoform 5 (identifier: P02686-5) [UniParc]FASTAAdd to basket
Also known as: MBP3, 18.5 kDa

The sequence of this isoform differs from the canonical sequence as follows:
     1-133: Missing.

Show »
Length:171
Mass (Da):18,591
Checksum:i8E73B56787611054
GO
Isoform 6 (identifier: P02686-6) [UniParc]FASTAAdd to basket
Also known as: MBP4, 17.2 kDa

The sequence of this isoform differs from the canonical sequence as follows:
     1-133: Missing.
     240-250: Missing.

Show »
Length:160
Mass (Da):17,343
Checksum:i0107AAD603FCD876
GO

Sequence cautioni

The sequence AAC41944 differs from that shown. Contaminating sequence. The C-terminus contains a Histidine tag.Curated
The sequence BAD92223 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAH10359 differs from that shown. wrong intron-exon boundaries.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti300P → T in CAG38771 (Ref. 8) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0033081 – 133Missing in isoform 3, isoform 4, isoform 5 and isoform 6. 7 PublicationsAdd BLAST133
Alternative sequenceiVSP_003309192K → KVPWLKPGRSPLPSHARSQP GLCNMYK in isoform 3 and isoform 4. 2 Publications1
Alternative sequenceiVSP_003311193 – 304DSHHP…PMARR → VSSEE in isoform 2. 2 PublicationsAdd BLAST112
Alternative sequenceiVSP_003310240 – 250Missing in isoform 4 and isoform 6. 4 PublicationsAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30047 mRNA Translation: AAA59559.1
M20009 mRNA Translation: AAA59561.1
M13577 mRNA Translation: AAA59562.1
M30516 mRNA Translation: AAA59563.1
M30515 mRNA Translation: AAA59564.1
X17286
, X17287, X17290, X17288, X17369, X17289 Genomic DNA Translation: CAA35179.1
L18862 Genomic DNA Translation: AAA72008.1
L18864 Genomic DNA Translation: AAA72009.1
L18865 Genomic DNA Translation: AAA72010.1
L18866 Genomic DNA Translation: AAA72011.1
L41657 Genomic DNA Translation: AAC41944.1 Sequence problems.
CR536534 mRNA Translation: CAG38771.1
CR541919 mRNA Translation: CAG46717.1
CR627018 mRNA Translation: CAH10359.1 Sequence problems.
AK128770 mRNA Translation: BAG54728.1
AK128788 mRNA Translation: BAG54734.1
AB208986 mRNA Translation: BAD92223.1 Different initiation.
AC018529 Genomic DNA No translation available.
AC093330 Genomic DNA No translation available.
BC008749 mRNA Translation: AAH08749.3
BC065248 mRNA Translation: AAH65248.1
BC080654 mRNA Translation: AAH80654.1
BC101771 mRNA Translation: AAI01772.1
BC101773 mRNA Translation: AAI01774.1
BC143348 mRNA Translation: AAI43349.1
BC143350 mRNA Translation: AAI43351.1
M63599 Genomic DNA Translation: AAA59560.1
CCDSiCCDS12011.1 [P02686-4]
CCDS32847.1 [P02686-3]
CCDS42448.1 [P02686-6]
CCDS42449.1 [P02686-5]
CCDS42450.1 [P02686-2]
PIRiA49635
S10482 MBHUB
RefSeqiNP_001020252.1, NM_001025081.1 [P02686-3]
NP_001020261.1, NM_001025090.1 [P02686-5]
NP_001020263.1, NM_001025092.1 [P02686-6]
NP_001020271.1, NM_001025100.1 [P02686-2]
NP_001020272.1, NM_001025101.1 [P02686-1]
NP_002376.1, NM_002385.2 [P02686-4]
XP_016881269.1, XM_017025780.1 [P02686-2]
UniGeneiHs.551713

Genome annotation databases

EnsembliENST00000355994; ENSP00000348273; ENSG00000197971 [P02686-1]
ENST00000359645; ENSP00000352667; ENSG00000197971 [P02686-4]
ENST00000382582; ENSP00000372025; ENSG00000197971 [P02686-3]
ENST00000397860; ENSP00000380958; ENSG00000197971 [P02686-2]
ENST00000397863; ENSP00000380961; ENSG00000197971 [P02686-2]
ENST00000397865; ENSP00000380963; ENSG00000197971 [P02686-6]
ENST00000397866; ENSP00000380964; ENSG00000197971 [P02686-5]
ENST00000580402; ENSP00000462223; ENSG00000197971 [P02686-1]
GeneIDi4155
KEGGihsa:4155
UCSCiuc002lml.4 human [P02686-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMBP_HUMAN
AccessioniPrimary (citable) accession number: P02686
Secondary accession number(s): A4FU54
, A6NI84, A8MY86, A8MYL4, B3KY66, B7ZKS2, B7ZKS4, Q15337, Q15338, Q15339, Q15340, Q59GX3, Q65ZS4, Q6AI64, Q6FH37, Q6FI04, Q6PK23
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: October 18, 2001
Last modified: July 18, 2018
This is version 208 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health