UniProtKB - P02652 (APOA2_HUMAN)
Protein
Apolipoprotein A-II
Gene
APOA2
Organism
Homo sapiens (Human)
Status
Functioni
May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.
Caution
The PDB entries that describe the 3D-structure of this protein have been obsoleted in response to US Office of Research Integrity Notice 2018-07782.1 Publication
GO - Molecular functioni
- apolipoprotein receptor binding Source: BHF-UCL
- cholesterol binding Source: BHF-UCL
- heat shock protein binding Source: CAFA
- high-density lipoprotein particle binding Source: GO_Central
- high-density lipoprotein particle receptor binding Source: BHF-UCL
- lipase inhibitor activity Source: BHF-UCL
- lipid binding Source: BHF-UCL
- lipid transporter activity Source: BHF-UCL
- phosphatidylcholine binding Source: BHF-UCL
- phosphatidylcholine-sterol O-acyltransferase activator activity Source: BHF-UCL
- phospholipid binding Source: BHF-UCL
- protein heterodimerization activity Source: UniProtKB
- protein homodimerization activity Source: BHF-UCL
- signaling receptor binding Source: ARUK-UCL
GO - Biological processi
- cellular protein metabolic process Source: Reactome
- cholesterol efflux Source: BHF-UCL
- cholesterol homeostasis Source: BHF-UCL
- cholesterol metabolic process Source: GO_Central
- cholesterol transport Source: GO_Central
- chylomicron assembly Source: Reactome
- chylomicron remodeling Source: Reactome
- diacylglycerol catabolic process Source: BHF-UCL
- high-density lipoprotein particle assembly Source: BHF-UCL
- high-density lipoprotein particle clearance Source: BHF-UCL
- high-density lipoprotein particle remodeling Source: BHF-UCL
- intermembrane lipid transfer Source: GOC
- lipoprotein metabolic process Source: GO_Central
- low-density lipoprotein particle remodeling Source: BHF-UCL
- negative regulation of cholesterol import Source: BHF-UCL
- negative regulation of cholesterol transport Source: BHF-UCL
- negative regulation of cholesterol transporter activity Source: BHF-UCL
- negative regulation of cytokine production involved in immune response Source: BHF-UCL
- negative regulation of lipase activity Source: BHF-UCL
- negative regulation of lipid catabolic process Source: BHF-UCL
- negative regulation of very-low-density lipoprotein particle remodeling Source: BHF-UCL
- peptidyl-methionine modification Source: UniProtKB
- phosphatidylcholine biosynthetic process Source: BHF-UCL
- phospholipid catabolic process Source: BHF-UCL
- phospholipid efflux Source: BHF-UCL
- positive regulation of cholesterol esterification Source: BHF-UCL
- positive regulation of interleukin-8 production Source: UniProtKB
- positive regulation of lipid catabolic process Source: BHF-UCL
- positive regulation of phagocytosis Source: UniProtKB
- post-translational protein modification Source: Reactome
- protein oxidation Source: UniProtKB
- protein stabilization Source: UniProtKB
- regulation of lipid metabolic process Source: Reactome
- regulation of protein stability Source: BHF-UCL
- response to glucose Source: BHF-UCL
- retinoid metabolic process Source: Reactome
- reverse cholesterol transport Source: BHF-UCL
- triglyceride metabolic process Source: BHF-UCL
- triglyceride-rich lipoprotein particle remodeling Source: BHF-UCL
- viral process Source: UniProtKB-KW
Keywordsi
Biological process | Host-virus interaction, Lipid transport, Transport |
Enzyme and pathway databases
PathwayCommonsi | P02652 |
Reactomei | R-HSA-1989781, PPARA activates gene expression R-HSA-381426, Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) R-HSA-8957275, Post-translational protein phosphorylation R-HSA-8963888, Chylomicron assembly R-HSA-8963901, Chylomicron remodeling R-HSA-975634, Retinoid metabolism and transport |
Names & Taxonomyi
Protein namesi | Recommended name: Apolipoprotein A-IIShort name: Apo-AII Short name: ApoA-II Alternative name(s): Apolipoprotein A2 Cleaved into the following 2 chains: Alternative name(s): Apolipoprotein A-II(1-76) |
Gene namesi | Name:APOA2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:601, APOA2 |
MIMi | 107670, gene+phenotype |
neXtProti | NX_P02652 |
VEuPathDBi | HostDB:ENSG00000158874.11 |
Subcellular locationi
Extracellular region or secreted
- Secreted 1 Publication
Cytosol
- cytosol Source: Reactome
Endoplasmic reticulum
- endoplasmic reticulum lumen Source: Reactome
Endosome
- early endosome Source: Reactome
Extracellular region or secreted
- blood microparticle Source: UniProtKB
- chylomicron Source: BHF-UCL
- extracellular exosome Source: UniProtKB
- extracellular region Source: Reactome
- high-density lipoprotein particle Source: BHF-UCL
- spherical high-density lipoprotein particle Source: BHF-UCL
- very-low-density lipoprotein particle Source: BHF-UCL
Keywords - Cellular componenti
HDL, SecretedPathology & Biotechi
Organism-specific databases
DisGeNETi | 336 |
MalaCardsi | APOA2 |
MIMi | 107670, gene+phenotype |
OpenTargetsi | ENSG00000158874 |
Orphaneti | 238269, AApoAII amyloidosis |
PharmGKBi | PA24886 |
Miscellaneous databases
Pharosi | P02652, Tbio |
Chemistry databases
DrugBanki | DB09130, Copper DB01593, Zinc DB14487, Zinc acetate |
Genetic variation databases
BioMutai | APOA2 |
DMDMi | 114000 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | 1 PublicationAdd BLAST | 18 | |
ChainiPRO_0000425351 | 19 – 100 | Proapolipoprotein A-IIAdd BLAST | 82 | |
ChainiPRO_0000002003 | 24 – 100 | Apolipoprotein A-II1 PublicationAdd BLAST | 77 | |
ChainiPRO_0000002004 | 24 – 99 | Truncated apolipoprotein A-II1 PublicationAdd BLAST | 76 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 24 | Pyrrolidone carboxylic acid2 Publications | 1 | |
Disulfide bondi | 29 | Interchain (with C-136 in APOD); in heterodimeric form1 Publication | ||
Modified residuei | 49 | Methionine sulfoxide1 Publication | 1 | |
Modified residuei | 54 | Phosphoserine; by FAM20C1 Publication | 1 | |
Modified residuei | 68 | Phosphoserine; by FAM20CCombined sources1 Publication | 1 |
Post-translational modificationi
Phosphorylation sites are present in the extracellular medium.1 Publication
Apolipoprotein A-II is O-glycosylated.1 Publication
Keywords - PTMi
Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Oxidation, Phosphoprotein, Pyrrolidone carboxylic acidProteomic databases
CPTACi | non-CPTAC-1073 non-CPTAC-1074 |
jPOSTi | P02652 |
MassIVEi | P02652 |
MaxQBi | P02652 |
PaxDbi | P02652 |
PeptideAtlasi | P02652 |
PRIDEi | P02652 |
ProteomicsDBi | 51538 |
TopDownProteomicsi | P02652 |
2D gel databases
SWISS-2DPAGEi | P02652 |
PTM databases
CarbonylDBi | P02652 |
GlyGeni | P02652, 3 sites, 3 O-linked glycans (3 sites) |
iPTMneti | P02652 |
PhosphoSitePlusi | P02652 |
Expressioni
Tissue specificityi
Plasma; synthesized in the liver and intestine.
Gene expression databases
Bgeei | ENSG00000158874, Expressed in liver and 119 other tissues |
ExpressionAtlasi | P02652, baseline and differential |
Genevisiblei | P02652, HS |
Organism-specific databases
HPAi | ENSG00000158874, Tissue enriched (liver) |
Interactioni
Subunit structurei
Monomer. Homodimer; disulfide-linked. Also forms a disulfide-linked heterodimer with APOD (PubMed:7918467).
Interacts with APOA1BP and NDRG1.
5 Publications(Microbial infection) Interacts with HCV core protein.
1 PublicationBinary interactionsi
Hide detailsP02652
GO - Molecular functioni
- apolipoprotein receptor binding Source: BHF-UCL
- heat shock protein binding Source: CAFA
- high-density lipoprotein particle receptor binding Source: BHF-UCL
- protein heterodimerization activity Source: UniProtKB
- protein homodimerization activity Source: BHF-UCL
- signaling receptor binding Source: ARUK-UCL
Protein-protein interaction databases
BioGRIDi | 106833, 36 interactors |
IntActi | P02652, 27 interactors |
MINTi | P02652 |
STRINGi | 9606.ENSP00000356969 |
Miscellaneous databases
RNActi | P02652, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 32 – 43 | O-glycosylated at one siteAdd BLAST | 12 |
Sequence similaritiesi
Belongs to the apolipoprotein A2 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG502SVYZ, Eukaryota |
GeneTreei | ENSGT00390000003306 |
InParanoidi | P02652 |
OrthoDBi | 1612564at2759 |
PhylomeDBi | P02652 |
TreeFami | TF338165 |
Family and domain databases
InterProi | View protein in InterPro IPR006801, ApoA-II IPR036172, ApoA-II_sf |
PANTHERi | PTHR11027, PTHR11027, 1 hit |
Pfami | View protein in Pfam PF04711, ApoA-II, 1 hit |
SUPFAMi | SSF82936, SSF82936, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 7 potential isoforms that are computationally mapped.Show allAlign All
P02652-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKLLAATVLL LTICSLEGAL VRRQAKEPCV ESLVSQYFQT VTDYGKDLME
60 70 80 90 100
KVKSPELQAE AKSYFEKSKE QLTPLIKKAG TELVNFLSYF VELGTQPATQ
Computationally mapped potential isoform sequencesi
There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketV9GYC1 | V9GYC1_HUMAN | Apolipoprotein A-II | APOA2 | 66 | Annotation score: | ||
V9GYG9 | V9GYG9_HUMAN | Apolipoprotein A-II | APOA2 | 95 | Annotation score: | ||
V9GYS1 | V9GYS1_HUMAN | Apolipoprotein A-II | APOA2 | 82 | Annotation score: | ||
V9GYM3 | V9GYM3_HUMAN | Apolipoprotein A-II | APOA2 | 133 | Annotation score: | ||
V9GYE3 | V9GYE3_HUMAN | Apolipoprotein A-II | APOA2 | 52 | Annotation score: | ||
V9GYT0 | V9GYT0_HUMAN | Apolipoprotein A-II | APOA2 hCG_1766509 | 72 | Annotation score: | ||
Q76EI7 | Q76EI7_HUMAN | Apolipoprotein A-II | APOA2 | 27 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 96 | Q → H in CAA28583 (PubMed:6428397).Curated | 1 |
Mass spectrometryi
Molecular mass is 17252 Da. Determined by ESI. Homodimer, without methionine sulfoxide.1 Publication
Molecular mass is 17269 Da. Determined by ESI. Homodimer, with 1 methionine sulfoxide, oxidation at Met-49.1 Publication
Molecular mass is 8823.4 Da. Determined by MALDI. Cysteinylated ApoA-II monomer.1 Publication
Molecular mass is 17293.4 Da. Determined by MALDI. Heterodimer with truncated apolipoprotein A-II.1 Publication
Molecular mass is 8578.3 Da. Determined by MALDI. Monomer.1 Publication
Molecular mass is 17166.2 Da. Determined by MALDI. Homodimer.1 Publication
Polymorphismi
A homozygous transition at position 1 of intron 3 of APOA2 results in deficiency of apolipoprotein A-II, without significant influence either on lipid and lipoprotein profiles or on the occurrence of coronary artery disease [MIMi:107670].1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04898 Genomic DNA Translation: CAA28583.1 X00955 mRNA Translation: CAA25467.1 X02905 Genomic DNA Translation: CAA26665.1 X02619 Genomic DNA Translation: CAA26474.1 M29882 mRNA Translation: AAA51701.1 AY100524 Genomic DNA Translation: AAM49807.1 AK312034 mRNA Translation: BAG34971.1 BT006786 mRNA Translation: AAP35432.1 AL590714 Genomic DNA No translation available. BC005282 mRNA Translation: AAH05282.1 |
CCDSi | CCDS1226.1 |
PIRi | A93586, LPHUA2 |
RefSeqi | NP_001634.1, NM_001643.1 |
Genome annotation databases
Ensembli | ENST00000367990; ENSP00000356969; ENSG00000158874 |
GeneIDi | 336 |
KEGGi | hsa:336 |
UCSCi | uc001fzc.2, human |
Similar proteinsi
Cross-referencesi
Web resourcesi
SHMPD The Singapore human mutation and polymorphism database |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04898 Genomic DNA Translation: CAA28583.1 X00955 mRNA Translation: CAA25467.1 X02905 Genomic DNA Translation: CAA26665.1 X02619 Genomic DNA Translation: CAA26474.1 M29882 mRNA Translation: AAA51701.1 AY100524 Genomic DNA Translation: AAM49807.1 AK312034 mRNA Translation: BAG34971.1 BT006786 mRNA Translation: AAP35432.1 AL590714 Genomic DNA No translation available. BC005282 mRNA Translation: AAH05282.1 |
CCDSi | CCDS1226.1 |
PIRi | A93586, LPHUA2 |
RefSeqi | NP_001634.1, NM_001643.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 106833, 36 interactors |
IntActi | P02652, 27 interactors |
MINTi | P02652 |
STRINGi | 9606.ENSP00000356969 |
Chemistry databases
DrugBanki | DB09130, Copper DB01593, Zinc DB14487, Zinc acetate |
PTM databases
CarbonylDBi | P02652 |
GlyGeni | P02652, 3 sites, 3 O-linked glycans (3 sites) |
iPTMneti | P02652 |
PhosphoSitePlusi | P02652 |
Genetic variation databases
BioMutai | APOA2 |
DMDMi | 114000 |
2D gel databases
SWISS-2DPAGEi | P02652 |
Proteomic databases
CPTACi | non-CPTAC-1073 non-CPTAC-1074 |
jPOSTi | P02652 |
MassIVEi | P02652 |
MaxQBi | P02652 |
PaxDbi | P02652 |
PeptideAtlasi | P02652 |
PRIDEi | P02652 |
ProteomicsDBi | 51538 |
TopDownProteomicsi | P02652 |
Protocols and materials databases
Antibodypediai | 20506, 491 antibodies |
DNASUi | 336 |
Genome annotation databases
Ensembli | ENST00000367990; ENSP00000356969; ENSG00000158874 |
GeneIDi | 336 |
KEGGi | hsa:336 |
UCSCi | uc001fzc.2, human |
Organism-specific databases
CTDi | 336 |
DisGeNETi | 336 |
GeneCardsi | APOA2 |
HGNCi | HGNC:601, APOA2 |
HPAi | ENSG00000158874, Tissue enriched (liver) |
MalaCardsi | APOA2 |
MIMi | 107670, gene+phenotype |
neXtProti | NX_P02652 |
OpenTargetsi | ENSG00000158874 |
Orphaneti | 238269, AApoAII amyloidosis |
PharmGKBi | PA24886 |
VEuPathDBi | HostDB:ENSG00000158874.11 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502SVYZ, Eukaryota |
GeneTreei | ENSGT00390000003306 |
InParanoidi | P02652 |
OrthoDBi | 1612564at2759 |
PhylomeDBi | P02652 |
TreeFami | TF338165 |
Enzyme and pathway databases
PathwayCommonsi | P02652 |
Reactomei | R-HSA-1989781, PPARA activates gene expression R-HSA-381426, Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) R-HSA-8957275, Post-translational protein phosphorylation R-HSA-8963888, Chylomicron assembly R-HSA-8963901, Chylomicron remodeling R-HSA-975634, Retinoid metabolism and transport |
Miscellaneous databases
BioGRID-ORCSi | 336, 7 hits in 873 CRISPR screens |
ChiTaRSi | APOA2, human |
EvolutionaryTracei | P02652 |
GeneWikii | APOA2 |
GenomeRNAii | 336 |
Pharosi | P02652, Tbio |
PROi | PR:P02652 |
RNActi | P02652, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000158874, Expressed in liver and 119 other tissues |
ExpressionAtlasi | P02652, baseline and differential |
Genevisiblei | P02652, HS |
Family and domain databases
InterProi | View protein in InterPro IPR006801, ApoA-II IPR036172, ApoA-II_sf |
PANTHERi | PTHR11027, PTHR11027, 1 hit |
Pfami | View protein in Pfam PF04711, ApoA-II, 1 hit |
SUPFAMi | SSF82936, SSF82936, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | APOA2_HUMAN | |
Accessioni | P02652Primary (citable) accession number: P02652 Secondary accession number(s): B2R524 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | August 13, 1987 | |
Last modified: | February 10, 2021 | |
This is version 230 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families