UniProtKB - P02651 (APOA4_RAT)
Protein
Apolipoprotein A-IV
Gene
Apoa4
Organism
Rattus norvegicus (Rat)
Status
Functioni
May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.
GO - Molecular functioni
- antioxidant activity Source: RGD
- cholesterol binding Source: GO_Central
- cholesterol transfer activity Source: RGD
- copper ion binding Source: RGD
- identical protein binding Source: RGD
- lipid binding Source: RGD
- phosphatidylcholine binding Source: RGD
- phosphatidylcholine-sterol O-acyltransferase activator activity Source: RGD
- phospholipid binding Source: GO_Central
- protein homodimerization activity Source: RGD
GO - Biological processi
- cholesterol biosynthetic process Source: GO_Central
- cholesterol efflux Source: RGD
- cholesterol homeostasis Source: RGD
- cholesterol metabolic process Source: RGD
- high-density lipoprotein particle assembly Source: GO_Central
- hydrogen peroxide catabolic process Source: RGD
- innate immune response in mucosa Source: RGD
- leukocyte cell-cell adhesion Source: RGD
- lipid catabolic process Source: RGD
- lipid homeostasis Source: RGD
- lipid transport Source: RGD
- lipoprotein metabolic process Source: GO_Central
- negative regulation of plasma lipoprotein oxidation Source: RGD
- peripheral nervous system axon regeneration Source: RGD
- phosphatidylcholine metabolic process Source: RGD
- phospholipid efflux Source: RGD
- positive regulation of cholesterol esterification Source: RGD
- positive regulation of fatty acid biosynthetic process Source: RGD
- positive regulation of lipid biosynthetic process Source: GO_Central
- positive regulation of lipoprotein lipase activity Source: RGD
- positive regulation of triglyceride catabolic process Source: RGD
- protein-lipid complex assembly Source: RGD
- regulation of cholesterol transport Source: RGD
- regulation of intestinal cholesterol absorption Source: RGD
- removal of superoxide radicals Source: RGD
- response to food Source: RGD
- response to lipid hydroperoxide Source: RGD
- response to stilbenoid Source: RGD
- response to triglyceride Source: RGD
- reverse cholesterol transport Source: RGD
- triglyceride homeostasis Source: GO_Central
- very-low-density lipoprotein particle remodeling Source: RGD
Keywordsi
Biological process | Lipid transport, Transport |
Enzyme and pathway databases
Reactomei | R-RNO-8963888, Chylomicron assembly R-RNO-8963901, Chylomicron remodeling R-RNO-975634, Retinoid metabolism and transport |
Names & Taxonomyi
Protein namesi | Recommended name: Apolipoprotein A-IVShort name: Apo-AIV Short name: ApoA-IV Alternative name(s): Apolipoprotein A4 |
Gene namesi | Name:Apoa4 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2132, Apoa4 |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- chylomicron Source: RGD
- extracellular space Source: RGD
- high-density lipoprotein particle Source: RGD
- very-low-density lipoprotein particle Source: RGD
Other locations
- synapse Source: SynGO
Keywords - Cellular componenti
Chylomicron, HDL, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | Add BLAST | 20 | |
ChainiPRO_0000001980 | 21 – 391 | Apolipoprotein A-IVAdd BLAST | 371 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 333 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PRIDEi | P02651 |
PTM databases
iPTMneti | P02651 |
PhosphoSitePlusi | P02651 |
Expressioni
Tissue specificityi
Secreted in plasma.
Interactioni
Subunit structurei
Homodimer.
By similarityGO - Molecular functioni
- identical protein binding Source: RGD
- protein homodimerization activity Source: RGD
Protein-protein interaction databases
IntActi | P02651, 2 interactors |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 33 – 54 | 1Add BLAST | 22 | |
Repeati | 60 – 81 | 2Add BLAST | 22 | |
Repeati | 82 – 103 | 3Add BLAST | 22 | |
Repeati | 115 – 136 | 4Add BLAST | 22 | |
Repeati | 137 – 158 | 5Add BLAST | 22 | |
Repeati | 159 – 180 | 6Add BLAST | 22 | |
Repeati | 181 – 202 | 7Add BLAST | 22 | |
Repeati | 203 – 224 | 8Add BLAST | 22 | |
Repeati | 225 – 246 | 9Add BLAST | 22 | |
Repeati | 247 – 268 | 10Add BLAST | 22 | |
Repeati | 269 – 286 | 11Add BLAST | 18 | |
Repeati | 287 – 308 | 12Add BLAST | 22 | |
Repeati | 309 – 330 | 13Add BLAST | 22 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 33 – 330 | 13 X 22 AA approximate tandem repeatsAdd BLAST | 298 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 374 – 385 | Gln/Glu-richAdd BLAST | 12 |
Domaini
Nine of the thirteen 22-amino acid tandem repeats (each 22-mer is actually a tandem array of two, A and B, related 11-mers) occurring in this sequence are predicted to be highly alpha-helical, and many of these helices are amphipathic. They may therefore serve as lipid-binding domains with lecithin:cholesterol acyltransferase (LCAT) activating abilities.
Sequence similaritiesi
Belongs to the apolipoprotein A1/A4/E family.Curated
Keywords - Domaini
Repeat, SignalPhylogenomic databases
InParanoidi | P02651 |
OrthoDBi | 1299087at2759 |
PhylomeDBi | P02651 |
Family and domain databases
InterProi | View protein in InterPro IPR000074, ApoA_E |
Pfami | View protein in Pfam PF01442, Apolipoprotein, 3 hits |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P02651-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFLKAVVLTV ALVAITGTQA EVTSDQVANV MWDYFTQLSN NAKEAVEQLQ
60 70 80 90 100
KTDVTQQLNT LFQDKLGNIN TYADDLQNKL VPFAVQLSGH LTKETERVRE
110 120 130 140 150
EIQKELEDLR ANMMPHANKV SQMFGDNVQK LQEHLRPYAT DLQAQINAQT
160 170 180 190 200
QDMKRQLTPY IQRMQTTIQD NVENLQSSMV PFANELKEKF NQNMEGLKGQ
210 220 230 240 250
LTPRANELKA TIDQNLEDLR SRLAPLAEGV QEKLNHQMEG LAFQMKKNAE
260 270 280 290 300
ELQTKVSTNI DQLQKNLAPL VEDVQSKLKG NTEGLQKSLE DLNKQLDQQV
310 320 330 340 350
EVFRRAVEPL GDKFNMALVQ QMEKFRQQLG SDSGDVESHL SFLEKNLREK
360 370 380 390
VSSFMSTLQK KGSPDQPLAL PLPEQVQEQV QEQVQPKPLE S
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JVX7 | A0A0G2JVX7_RAT | Apolipoprotein A-IV | Apoa4 | 392 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 253 | Q → H. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M00002 mRNA Translation: AAA85909.1 J02588 Genomic DNA Translation: AAA40747.1 M13508 Genomic DNA Translation: AAA40748.1 BC091159 mRNA Translation: AAH91159.1 |
PIRi | A03095, LPRTA4 |
RefSeqi | NP_036869.1, NM_012737.1 |
Genome annotation databases
GeneIDi | 25080 |
KEGGi | rno:25080 |
UCSCi | RGD:2132, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M00002 mRNA Translation: AAA85909.1 J02588 Genomic DNA Translation: AAA40747.1 M13508 Genomic DNA Translation: AAA40748.1 BC091159 mRNA Translation: AAH91159.1 |
PIRi | A03095, LPRTA4 |
RefSeqi | NP_036869.1, NM_012737.1 |
3D structure databases
SMRi | P02651 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P02651, 2 interactors |
PTM databases
iPTMneti | P02651 |
PhosphoSitePlusi | P02651 |
Proteomic databases
PRIDEi | P02651 |
Genome annotation databases
GeneIDi | 25080 |
KEGGi | rno:25080 |
UCSCi | RGD:2132, rat |
Organism-specific databases
CTDi | 337 |
RGDi | 2132, Apoa4 |
Phylogenomic databases
InParanoidi | P02651 |
OrthoDBi | 1299087at2759 |
PhylomeDBi | P02651 |
Enzyme and pathway databases
Reactomei | R-RNO-8963888, Chylomicron assembly R-RNO-8963901, Chylomicron remodeling R-RNO-975634, Retinoid metabolism and transport |
Miscellaneous databases
PROi | PR:P02651 |
Family and domain databases
InterProi | View protein in InterPro IPR000074, ApoA_E |
Pfami | View protein in Pfam PF01442, Apolipoprotein, 3 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | APOA4_RAT | |
Accessioni | P02651Primary (citable) accession number: P02651 Secondary accession number(s): Q5BK92 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 1, 1989 | |
Last modified: | December 2, 2020 | |
This is version 125 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families