UniProtKB - P02557 (TBB_YEAST)
Protein
Tubulin beta chain
Gene
TUB2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Tubulin is the major constituent of microtubules (PubMed:28013290). It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.1 Publication
Miscellaneous
Rhizoxin, an antibiotic that exhibits potent anti-mitotic activity against most eukaryotic cells except for yeasts, binds to beta tubulin.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 140 – 146 | GTPSequence analysis | 7 |
GO - Molecular functioni
- GTPase activity Source: InterPro
- GTP binding Source: GO_Central
- structural constituent of cytoskeleton Source: GO_Central
GO - Biological processi
- cytoplasmic microtubule organization Source: SGD
- cytoskeleton organization Source: UniProtKB
- homologous chromosome segregation Source: SGD
- microtubule-based process Source: UniProtKB
- microtubule cytoskeleton organization Source: GO_Central
- mitotic cell cycle Source: GO_Central
- mitotic sister chromatid segregation Source: SGD
- mitotic spindle assembly Source: SGD
- mitotic spindle elongation Source: SGD
- nuclear migration along microtubule Source: SGD
- positive regulation of intracellular protein transport Source: UniProtKB
- response to antibiotic Source: UniProtKB-KW
Keywordsi
Biological process | Antibiotic resistance |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-SCE-5617833, Cilium Assembly R-SCE-6798695, Neutrophil degranulation |
Names & Taxonomyi
Protein namesi | Recommended name: Tubulin beta chainAlternative name(s): Beta-tubulin |
Gene namesi | Name:TUB2 Ordered Locus Names:YFL037W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000001857, TUB2 |
VEuPathDBi | FungiDB:YFL037W |
Subcellular locationi
Cytoskeleton
- cytoskeleton 1 Publication
Note: Colocalizes with kinesin KIP3 at the plus ends of growing microtubules and along the microtubule lattice.1 Publication
Cytoskeleton
- cytoplasmic microtubule Source: SGD
- kinetochore microtubule Source: SGD
- microtubule Source: GO_Central
- nuclear microtubule Source: SGD
- tubulin complex Source: SGD
Nucleus
- nuclear microtubule Source: SGD
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, MicrotubulePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 100 | V → N: Becomes sensitive to rhizoxin. | 1 | |
Mutagenesisi | 390 | K → Q: Decreased microtubule stability. 1 Publication | 1 | |
Mutagenesisi | 421 | E → K: Increased microtubule polymerization and depolymerization rates. Increased microtubule stability. Decreased kinesin KIP3 subcellular location at microtubule plus ends. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000048443 | 1 – 457 | Tubulin beta chainAdd BLAST | 457 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 278 | PhosphoserineCombined sources | 1 | |
Modified residuei | 280 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P02557 |
PaxDbi | P02557 |
PRIDEi | P02557 |
PTM databases
iPTMneti | P02557 |
Interactioni
Subunit structurei
Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.
Binary interactionsi
P02557
With | #Exp. | IntAct |
---|---|---|
RBL2 [P48606] | 2 | EBI-18986,EBI-18991 |
Protein-protein interaction databases
BioGRIDi | 31109, 447 interactors |
ComplexPortali | CPX-1424, Tubulin alpha-beta heterodimeric complex, TUB1 variant CPX-1425, Tubulin alpha-beta heterodimeric complex, TUB3 variant |
DIPi | DIP-2340N |
IntActi | P02557, 339 interactors |
MINTi | P02557 |
STRINGi | 4932.YFL037W |
Miscellaneous databases
RNActi | P02557, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P02557 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the tubulin family.Curated
Phylogenomic databases
eggNOGi | KOG1375, Eukaryota |
GeneTreei | ENSGT00940000154394 |
HOGENOMi | CLU_015718_1_1_1 |
InParanoidi | P02557 |
OMAi | EVGANKY |
Family and domain databases
Gene3Di | 1.10.287.600, 1 hit 3.30.1330.20, 1 hit 3.40.50.1440, 1 hit |
InterProi | View protein in InterPro IPR013838, Beta-tubulin_BS IPR002453, Beta_tubulin IPR008280, Tub_FtsZ_C IPR000217, Tubulin IPR018316, Tubulin/FtsZ_2-layer-sand-dom IPR037103, Tubulin/FtsZ_C_sf IPR036525, Tubulin/FtsZ_GTPase_sf IPR023123, Tubulin_C IPR017975, Tubulin_CS IPR003008, Tubulin_FtsZ_GTPase |
PANTHERi | PTHR11588, PTHR11588, 1 hit |
Pfami | View protein in Pfam PF00091, Tubulin, 1 hit PF03953, Tubulin_C, 1 hit |
PRINTSi | PR01163, BETATUBULIN PR01161, TUBULIN |
SMARTi | View protein in SMART SM00864, Tubulin, 1 hit SM00865, Tubulin_C, 1 hit |
SUPFAMi | SSF52490, SSF52490, 1 hit SSF55307, SSF55307, 1 hit |
PROSITEi | View protein in PROSITE PS00227, TUBULIN, 1 hit PS00228, TUBULIN_B_AUTOREG, 1 hit |
i Sequence
Sequence statusi: Complete.
P02557-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MREIIHISTG QCGNQIGAAF WETICGEHGL DFNGTYHGHD DIQKERLNVY
60 70 80 90 100
FNEASSGKWV PRSINVDLEP GTIDAVRNSA IGNLFRPDNY IFGQSSAGNV
110 120 130 140 150
WAKGHYTEGA ELVDSVMDVI RREAEGCDSL QGFQITHSLG GGTGSGMGTL
160 170 180 190 200
LISKIREEFP DRMMATFSVL PSPKTSDTVV EPYNATLSVH QLVEHSDETF
210 220 230 240 250
CIDNEALYDI CQRTLKLNQP SYGDLNNLVS SVMSGVTTSL RYPGQLNSDL
260 270 280 290 300
RKLAVNLVPF PRLHFFMVGY APLTAIGSQS FRSLTVPELT QQMFDAKNMM
310 320 330 340 350
AAADPRNGRY LTVAAFFRGK VSVKEVEDEM HKVQSKNSDY FVEWIPNNVQ
360 370 380 390 400
TAVCSVAPQG LDMAATFIAN STSIQELFKR VGDQFSAMFK RKAFLHWYTS
410 420 430 440 450
EGMDELEFSE AESNMNDLVS EYQQYQEATV EDDEEVDENG DFGAPQNQDE
PITENFE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 9 | T → A in CAA24603 (PubMed:6380751).Curated | 1 | |
Sequence conflicti | 12 | C → Y in CAA24603 (PubMed:6380751).Curated | 1 | |
Sequence conflicti | 71 | G → W in CAA24603 (PubMed:6380751).Curated | 1 | |
Sequence conflicti | 152 | I → F in CAA24603 (PubMed:6380751).Curated | 1 | |
Sequence conflicti | 156 | R → K in CAA24603 (PubMed:6380751).Curated | 1 | |
Sequence conflicti | 159 | F → L in CAA24603 (PubMed:6380751).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V01296 Genomic DNA Translation: CAA24603.1 D50617 Genomic DNA Translation: BAA09202.1 X00209 Genomic DNA Translation: CAA25035.1 BK006940 Genomic DNA Translation: DAA12403.1 |
PIRi | S56217, UBBYB |
RefSeqi | NP_116616.1, NM_001179929.1 |
Genome annotation databases
EnsemblFungii | YFL037W_mRNA; YFL037W; YFL037W |
GeneIDi | 850506 |
KEGGi | sce:YFL037W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V01296 Genomic DNA Translation: CAA24603.1 D50617 Genomic DNA Translation: BAA09202.1 X00209 Genomic DNA Translation: CAA25035.1 BK006940 Genomic DNA Translation: DAA12403.1 |
PIRi | S56217, UBBYB |
RefSeqi | NP_116616.1, NM_001179929.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4FFB | X-ray | 2.88 | B | 1-457 | [»] | |
4U3J | X-ray | 2.81 | B | 1-457 | [»] | |
5W3F | electron microscopy | 3.70 | B | 1-457 | [»] | |
5W3H | electron microscopy | 4.00 | B | 1-457 | [»] | |
5W3J | electron microscopy | 4.00 | B | 1-457 | [»] | |
SMRi | P02557 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 31109, 447 interactors |
ComplexPortali | CPX-1424, Tubulin alpha-beta heterodimeric complex, TUB1 variant CPX-1425, Tubulin alpha-beta heterodimeric complex, TUB3 variant |
DIPi | DIP-2340N |
IntActi | P02557, 339 interactors |
MINTi | P02557 |
STRINGi | 4932.YFL037W |
PTM databases
iPTMneti | P02557 |
Proteomic databases
MaxQBi | P02557 |
PaxDbi | P02557 |
PRIDEi | P02557 |
Genome annotation databases
EnsemblFungii | YFL037W_mRNA; YFL037W; YFL037W |
GeneIDi | 850506 |
KEGGi | sce:YFL037W |
Organism-specific databases
SGDi | S000001857, TUB2 |
VEuPathDBi | FungiDB:YFL037W |
Phylogenomic databases
eggNOGi | KOG1375, Eukaryota |
GeneTreei | ENSGT00940000154394 |
HOGENOMi | CLU_015718_1_1_1 |
InParanoidi | P02557 |
OMAi | EVGANKY |
Enzyme and pathway databases
Reactomei | R-SCE-5617833, Cilium Assembly R-SCE-6798695, Neutrophil degranulation |
Miscellaneous databases
PROi | PR:P02557 |
RNActi | P02557, protein |
Family and domain databases
Gene3Di | 1.10.287.600, 1 hit 3.30.1330.20, 1 hit 3.40.50.1440, 1 hit |
InterProi | View protein in InterPro IPR013838, Beta-tubulin_BS IPR002453, Beta_tubulin IPR008280, Tub_FtsZ_C IPR000217, Tubulin IPR018316, Tubulin/FtsZ_2-layer-sand-dom IPR037103, Tubulin/FtsZ_C_sf IPR036525, Tubulin/FtsZ_GTPase_sf IPR023123, Tubulin_C IPR017975, Tubulin_CS IPR003008, Tubulin_FtsZ_GTPase |
PANTHERi | PTHR11588, PTHR11588, 1 hit |
Pfami | View protein in Pfam PF00091, Tubulin, 1 hit PF03953, Tubulin_C, 1 hit |
PRINTSi | PR01163, BETATUBULIN PR01161, TUBULIN |
SMARTi | View protein in SMART SM00864, Tubulin, 1 hit SM00865, Tubulin_C, 1 hit |
SUPFAMi | SSF52490, SSF52490, 1 hit SSF55307, SSF55307, 1 hit |
PROSITEi | View protein in PROSITE PS00227, TUBULIN, 1 hit PS00228, TUBULIN_B_AUTOREG, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TBB_YEAST | |
Accessioni | P02557Primary (citable) accession number: P02557 Secondary accession number(s): D6VTJ3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | November 1, 1995 | |
Last modified: | February 10, 2021 | |
This is version 199 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VI
Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families