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Protein

Keratin, type I cytoskeletal 10

Gene

Krt10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in the establishment of the epidermal barrier on plantar skin.1 Publication
(Microbial infection) Acts as a mediator of S.aureus adherence to desquamated nasal epithelial cells via clfB, and hence may play a role in nasal colonization.1 Publication

Miscellaneous

There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei396Stutter1

GO - Molecular functioni

  • cytoskeletal protein binding Source: MGI
  • protein heterodimerization activity Source: UniProtKB
  • structural molecule activity Source: InterPro

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
Keratin, type I cytoskeletal 10
Alternative name(s):
56 kDa cytokeratin
Cytokeratin-10
Short name:
CK-10
Keratin, type I cytoskeletal 59 kDa
Keratin-10
Short name:
K10
Gene namesi
Name:Krt10
Synonyms:Krt1-10
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:96685 Krt10

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Intermediate filament, Keratin

Pathology & Biotechi

Disruption phenotypei

Mice are viable and display no differences in size and body weight. Show acanthosis, hyperkeratosis and scaling of the stratum corneum in plantar skin. Show no epidermal aberrations of the footpads. Double knockout mice of KRT10 and KRT2 are viable and display no differences in size and body weight. Show a more severe plantar epidermis phenotype as in single KRT10 knockout mice.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000636431 – 570Keratin, type I cytoskeletal 10Add BLAST570

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei15PhosphoserineBy similarity1
Modified residuei17PhosphoserineBy similarity1
Modified residuei32Asymmetric dimethylarginine; alternateCombined sources1
Modified residuei32Omega-N-methylarginine; alternateCombined sources1
Modified residuei34PhosphoserineCombined sources1
Modified residuei45PhosphoserineBy similarity1
Modified residuei48PhosphoserineCombined sources1
Modified residuei168PhosphoserineBy similarity1
Disulfide bondi399InterchainBy similarity

Keywords - PTMi

Disulfide bond, Methylation, Phosphoprotein

Proteomic databases

PRIDEiP02535

PTM databases

iPTMnetiP02535
PhosphoSitePlusiP02535

Expressioni

Tissue specificityi

Expressed in the suprabasal layers of the epidermis throughout the entire sole (at protein level).1 Publication

Gene expression databases

CleanExiMM_KRT10

Interactioni

Subunit structurei

(Microbial infection) Interacts (via C-terminal tail domain) with the S.aureus clumping factor, clfB; this interaction probably mediates S.aureus attachment to the highly keratinized squamous epithelial cells from the nasal cavity.1 Publication
Heterotetramer of two type I and two type II keratins. Heterodimer with KRT1. Two heterodimers of KRT1 and KRT10 form a heterotetramer. The KRT10 subunit in the heterotetramer is probably disulfide-linked.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
ChukQ60680-16EBI-646288,EBI-646260

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201017, 10 interactors
IntActiP02535, 2 interactors
MINTiP02535

Structurei

3D structure databases

ProteinModelPortaliP02535
SMRiP02535
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini144 – 458IF rodPROSITE-ProRule annotationAdd BLAST315

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 143HeadAdd BLAST143
Regioni144 – 179Coil 1AAdd BLAST36
Regioni180 – 200Linker 1Add BLAST21
Regioni201 – 292Coil 1BAdd BLAST92
Regioni293 – 315Linker 12Add BLAST23
Regioni316 – 454Coil 2Add BLAST139
Regioni455 – 570TailAdd BLAST116

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi453 – 565Gly/Ser-richAdd BLAST113

Sequence similaritiesi

Belongs to the intermediate filament family.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

HOVERGENiHBG013015
InParanoidiP02535
KOiK07604
PhylomeDBiP02535

Family and domain databases

InterProiView protein in InterPro
IPR001664 IF
IPR018039 IF_conserved
IPR039008 IF_rod_dom
IPR002957 Keratin_I
PANTHERiPTHR23239 PTHR23239, 2 hits
PfamiView protein in Pfam
PF00038 Filament, 1 hit
PRINTSiPR01248 TYPE1KERATIN
SMARTiView protein in SMART
SM01391 Filament, 1 hit
PROSITEiView protein in PROSITE
PS00226 IF_ROD_1, 1 hit
PS51842 IF_ROD_2, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P02535-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSVLYSSSSK QFSSSRSGGG GGGGSVRVSS TRGSLGGGYS SGGFSGGSFS
60 70 80 90 100
RGSSGGGCFG GSSGGYGGFG GGGSFGGGYG GSSFGGGYGG SSFGGGYGGS
110 120 130 140 150
SFGGAGFGGG GSFGGGSFGG GSYGGGFGGG GFGGDGGSLL SGNGRVTMQN
160 170 180 190 200
LNDRLASYMD KVRALEESNY ELEGKIKEWY EKHGNSSQRE PRDYSKYYKT
210 220 230 240 250
IEDLKGQILT LTTDNANVLL QIDNARLAAD DFRLKYENEV TLRQSVEADI
260 270 280 290 300
NGLRRVLDEL TLSKSDLEMQ IESLNEELAY LKKNHEEEMR DLQNVSTGDV
310 320 330 340 350
NVEMNAAPGV DLTQLLNNMR NQYEQLAEKN RKDAEEWFNQ KSKELTTEID
360 370 380 390 400
SNIEQMSSHK SEITELRRTV QGLEIELQSQ LALKQSLEAS LAETEGRYCV
410 420 430 440 450
QLSQIQSQIS ALEEQLQQIR AETECQNAEY QQLLDIKTRL ENEIQTYRSL
460 470 480 490 500
LEGEGSSSGG GGGRRGGSGG GSYGGSSGGG SYGGSSGGGG SYGGSSGGGG
510 520 530 540 550
SYGGGSSGGG SHGGSSGGGY GGGSSSGGAG GHGGSSGGGY GGGSSSGGQG
560 570
GSGGFKSSGG GDQSSKGPRY
Length:570
Mass (Da):57,770
Last modified:January 23, 2007 - v3
Checksum:iEE60EA87DE7770E0
GO
Isoform 2 (identifier: P02535-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     97-106: YGGSSFGGAG → S
     144-145: GR → EK

Note: No experimental confirmation available.
Show »
Length:561
Mass (Da):57,060
Checksum:i8EEF036A0FA03831
GO
Isoform 3 (identifier: P02535-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     97-106: YGGSSFGGAG → S
     144-145: GR → EK
     465-471: RGGSGGG → PRRQPRR
     472-570: Missing.

Note: No experimental confirmation available.
Show »
Length:462
Mass (Da):49,501
Checksum:iD7A0BC767157B99D
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2A513A2A513_MOUSE
Keratin, type I cytoskeletal 10
Krt10
561Annotation score:
A0A1B0GSR7A0A1B0GSR7_MOUSE
Keratin, type I cytoskeletal 10
Krt10
97Annotation score:

Sequence cautioni

The sequence BAB29296 differs from that shown. Reason: Frameshift at positions 96 and 122.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4L → R in AAF65456 (Ref. 4) Curated1
Sequence conflicti6S → C in CAA24214 (PubMed:6188955).Curated1
Sequence conflicti23G → GGG (Ref. 4) Curated1
Sequence conflicti25S → F in CAA24214 (PubMed:6188955).Curated1
Sequence conflicti29S → F in CAA24214 (PubMed:6188955).Curated1
Sequence conflicti39Y → L in CAA24214 (PubMed:6188955).Curated1
Sequence conflicti42G → E in AAA39391 (PubMed:2581944).Curated1
Sequence conflicti46G → R in BAB29296 (PubMed:16141072).Curated1
Sequence conflicti94G → V in BAB29296 (PubMed:16141072).Curated1
Sequence conflicti105 – 106AG → GS in CAA24214 (PubMed:6188955).Curated2
Sequence conflicti111Missing (PubMed:6188955).Curated1
Sequence conflicti122 – 123SY → GC in CAA24214 (PubMed:6188955).Curated2
Sequence conflicti138S → G in CAA24214 (PubMed:6188955).Curated1
Sequence conflicti149Q → R in CAA24214 (PubMed:6188955).Curated1
Sequence conflicti179 – 188WYEKHGNSSQ → VVREARQLKP in CAA24214 (PubMed:6188955).Curated10
Sequence conflicti264 – 269KSDLEM → QSVLEL in CAA24214 (PubMed:6188955).Curated6
Sequence conflicti277E → G in BAC38210 (PubMed:16141072).Curated1
Sequence conflicti285H → L in CAA24214 (PubMed:6188955).Curated1
Sequence conflicti354E → A in CAA24214 (PubMed:6188955).Curated1
Sequence conflicti395 – 400EGRYCV → VESLLR in CAA24214 (PubMed:6188955).Curated6
Sequence conflicti465R → G in BAB29296 (PubMed:16141072).Curated1
Sequence conflicti465R → G in BAC36371 (PubMed:16141072).Curated1
Sequence conflicti465R → G in BAC38210 (PubMed:16141072).Curated1
Sequence conflicti466 – 467GG → AD in BAB29296 (PubMed:16141072).Curated2
Sequence conflicti469G → H in BAB29296 (PubMed:16141072).Curated1
Sequence conflicti469G → H in BAC36371 (PubMed:16141072).Curated1
Sequence conflicti469G → H in BAC38210 (PubMed:16141072).Curated1
Sequence conflicti509 – 515GGSHGGS → CGGRGGG in CAA24214 (PubMed:6188955).Curated7
Sequence conflicti524S → G in CAA24214 (PubMed:6188955).Curated1
Sequence conflicti532H → R in CAA24214 (PubMed:6188955).Curated1
Sequence conflicti535S → G in CAA24214 (PubMed:6188955).Curated1
Sequence conflicti544S → G in CAA24214 (PubMed:6188955).Curated1
Sequence conflicti548 – 549GQ → RR in CAA24214 (PubMed:6188955).Curated2
Sequence conflicti556 – 557KS → SGT in CAA24214 (PubMed:6188955).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02110097 – 106YGGSSFGGAG → S in isoform 2 and isoform 3. 1 Publication10
Alternative sequenceiVSP_021101144 – 145GR → EK in isoform 2 and isoform 3. 1 Publication2
Alternative sequenceiVSP_021102465 – 471RGGSGGG → PRRQPRR in isoform 3. 1 Publication7
Alternative sequenceiVSP_021103472 – 570Missing in isoform 3. 1 PublicationAdd BLAST99

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L00193, M10081 Genomic DNA Translation: AAA39391.1
V00830 mRNA Translation: CAA24214.1
AK014360 mRNA Translation: BAB29296.1 Frameshift.
AK076508 mRNA Translation: BAC36371.1
AK081402 mRNA Translation: BAC38210.1
AK081914 mRNA Translation: BAC38369.1
AF245658 Genomic DNA Translation: AAF65456.1
CCDSiCCDS25380.1 [P02535-2]
PIRiA02940 KRMSE1
S07330
RefSeqiNP_034790.2, NM_010660.2
UniGeneiMm.22662

Genome annotation databases

GeneIDi16661
KEGGimmu:16661

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L00193, M10081 Genomic DNA Translation: AAA39391.1
V00830 mRNA Translation: CAA24214.1
AK014360 mRNA Translation: BAB29296.1 Frameshift.
AK076508 mRNA Translation: BAC36371.1
AK081402 mRNA Translation: BAC38210.1
AK081914 mRNA Translation: BAC38369.1
AF245658 Genomic DNA Translation: AAF65456.1
CCDSiCCDS25380.1 [P02535-2]
PIRiA02940 KRMSE1
S07330
RefSeqiNP_034790.2, NM_010660.2
UniGeneiMm.22662

3D structure databases

ProteinModelPortaliP02535
SMRiP02535
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201017, 10 interactors
IntActiP02535, 2 interactors
MINTiP02535

PTM databases

iPTMnetiP02535
PhosphoSitePlusiP02535

Proteomic databases

PRIDEiP02535

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi16661
KEGGimmu:16661

Organism-specific databases

CTDi3858
MGIiMGI:96685 Krt10

Phylogenomic databases

HOVERGENiHBG013015
InParanoidiP02535
KOiK07604
PhylomeDBiP02535

Miscellaneous databases

ChiTaRSiKrt10 mouse
PROiPR:P02535
SOURCEiSearch...

Gene expression databases

CleanExiMM_KRT10

Family and domain databases

InterProiView protein in InterPro
IPR001664 IF
IPR018039 IF_conserved
IPR039008 IF_rod_dom
IPR002957 Keratin_I
PANTHERiPTHR23239 PTHR23239, 2 hits
PfamiView protein in Pfam
PF00038 Filament, 1 hit
PRINTSiPR01248 TYPE1KERATIN
SMARTiView protein in SMART
SM01391 Filament, 1 hit
PROSITEiView protein in PROSITE
PS00226 IF_ROD_1, 1 hit
PS51842 IF_ROD_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiK1C10_MOUSE
AccessioniPrimary (citable) accession number: P02535
Secondary accession number(s): P08731
, Q8BUX3, Q8BV09, Q8BVU3, Q9CXH6, Q9JKB4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: July 18, 2018
This is version 137 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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