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Protein

60S acidic ribosomal protein P2-beta

Gene

RPP2B

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.1 Publication

Miscellaneous

The 4 small acidic ribosomal P-proteins from yeast can be classified into two couples of similar but not identical sequences. Each couple (P1A/P1B and P2A/P2B) is distinctly related to one of the two acidic ribosomal P-proteins P1/P2 present in multicellular organisms.1 Publication
Present with 602000 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • protein kinase activator activity Source: SGD
  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • positive regulation of protein kinase activity Source: SGD
  • translational elongation Source: InterPro

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-29930-MONOMER
ReactomeiR-SCE-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-SCE-1799339 SRP-dependent cotranslational protein targeting to membrane
R-SCE-72689 Formation of a pool of free 40S subunits
R-SCE-72706 GTP hydrolysis and joining of the 60S ribosomal subunit
R-SCE-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-SCE-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Names & Taxonomyi

Protein namesi
Recommended name:
60S acidic ribosomal protein P2-beta1 Publication
Alternative name(s):
L12eIA
L45
Large ribosomal subunit protein P2-B1 Publication
Short name:
P2B
YL44C
YP2beta
YPA1
Gene namesi
Name:RPP2B1 Publication
Synonyms:L12EIA, RPL45, RPLA4
Ordered Locus Names:YDR382W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR382W
SGDiS000002790 RPP2B

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001576821 – 11060S acidic ribosomal protein P2-betaAdd BLAST110

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei29PhosphoserineCombined sources1
Cross-linki49Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei100PhosphoserineCombined sources1

Post-translational modificationi

The N-terminus is not modified.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP02400
PaxDbiP02400
PRIDEiP02400
TopDownProteomicsiP02400

PTM databases

iPTMnetiP02400

Interactioni

Subunit structurei

Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes) (PubMed:9559554, PubMed:22096102). The 5 acidic ribosomal P-proteins form the stalk structure of the 60S subunit. They are organized as a pentameric complex in which uL10/P0 interacts with 2 heterodimers, P1A-P2B and P1B-P2A (PubMed:16573688, PubMed:11431471).1 Publication3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RPP1AP053182EBI-15464,EBI-15452

Protein-protein interaction databases

BioGridi32443, 374 interactors
DIPiDIP-580N
IntActiP02400, 35 interactors
MINTiP02400
STRINGi4932.YDR382W

Structurei

3D structure databases

ProteinModelPortaliP02400
SMRiP02400
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02400

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000074828
HOGENOMiHOG000229897
InParanoidiP02400
KOiK02943
OMAiNEVMNSG
OrthoDBiEOG092C5X8C

Family and domain databases

Gene3Di1.10.10.1410, 1 hit
HAMAPiMF_01478 Ribosomal_L12_arch, 1 hit
InterProiView protein in InterPro
IPR038716 P1/P2_N_sf
IPR027534 Ribosomal_L12

Sequencei

Sequence statusi: Complete.

P02400-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKYLAAYLLL VQGGNAAPSA ADIKAVVESV GAEVDEARIN ELLSSLEGKG
60 70 80 90 100
SLEEIIAEGQ KKFATVPTGG ASSAAAGAAG AAAGGDAAEE EKEEEAKEES
110
DDDMGFGLFD
Length:110
Mass (Da):11,050
Last modified:July 1, 1989 - v2
Checksum:iEC45406CB5F199F4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti75 – 78AAGA → GPAS AA sequence (PubMed:7030402).Curated4
Sequence conflicti86 – 87DA → GD AA sequence (PubMed:7030402).Curated2
Sequence conflicti89E → A AA sequence (PubMed:7030402).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03761 Genomic DNA Translation: AAA34972.1
M26505 Genomic DNA Translation: AAA34732.1
U28373 Genomic DNA Translation: AAB64818.1
U32274 Genomic DNA Translation: AAB64824.1
BK006938 Genomic DNA Translation: DAA12226.1
PIRiA35109 R5BYA1
RefSeqiNP_010670.3, NM_001180690.3

Genome annotation databases

EnsemblFungiiYDR382W; YDR382W; YDR382W
GeneIDi851990
KEGGisce:YDR382W

Similar proteinsi

Entry informationi

Entry nameiRLA4_YEAST
AccessioniPrimary (citable) accession number: P02400
Secondary accession number(s): D6VT16
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 1, 1989
Last modified: September 12, 2018
This is version 186 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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