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Protein

30S ribosomal protein S7

Gene

rpsG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit (PubMed:2461734). Is located at the subunit interface close to the decoding center, where it has been shown to contact mRNA (PubMed:10606263). Has been shown to contact tRNA in both the P and E sites; it probably blocks exit of the E site tRNA (PubMed:8524654).3 Publications
Protein S7 is also a translational repressor protein; it regulates the expression of the str operon members to different degrees by binding to its mRNA.1 Publication

Miscellaneous

The strain K12 sequence is shown.
Has been predicted to contact the N-terminal domain of IF-3 based on footprint studies; exactly how IF-3 interacts with the 30S subunit is controversial (PubMed:7000779, PubMed:6349681, PubMed:11684020).3 Publications

GO - Molecular functioni

  • mRNA binding Source: CAFA
  • rRNA binding Source: EcoCyc
  • structural constituent of ribosome Source: CAFA
  • tRNA binding Source: UniProtKB-KW

GO - Biological processi

  • negative regulation of translation Source: CAFA
  • ribosomal small subunit assembly Source: CAFA
  • translation Source: InterPro

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein, RNA-binding, rRNA-binding, tRNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10906-MONOMER
MetaCyc:EG10906-MONOMER

Protein family/group databases

MoonProtiP02359

Names & Taxonomyi

Protein namesi
Recommended name:
30S ribosomal protein S7
Alternative name(s):
Small ribosomal subunit protein uS71 Publication
Gene namesi
Name:rpsG
Ordered Locus Names:b3341, JW3303
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10906 rpsG

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • cytosolic small ribosomal subunit Source: CAFA
  • membrane Source: UniProtKB

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi2 – 18Missing : Defective in ribosome assembly; accumulates to abnormally high levels on polysomes; significantly decreases affinity for its own mRNA. 1 PublicationAdd BLAST17
Mutagenesisi36K → A or E: Defective in ribosome assembly. 1 Publication1
Mutagenesisi116M → G: Significantly decreases affinity for its own mRNA. 1 Publication1

Chemistry databases

DrugBankiDB00759 Tetracycline

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001242592 – 17930S ribosomal protein S7Add BLAST178

Proteomic databases

EPDiP02359
PaxDbiP02359
PRIDEiP02359

Interactioni

Subunit structurei

Part of the 30S ribosomal subunit (PubMed:385062, PubMed:7556101, PubMed:10094780, PubMed:12809609, PubMed:16272117, PubMed:27934701, PubMed:12244297, PubMed:27906160, PubMed:27906161, PubMed:28077875). Contacts proteins S9 and S11 (By similarity). Cross-links to IF3 and the P and E site tRNAs (PubMed:7000779, PubMed:6349681, PubMed:8524654).By similarity2 Publications11 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

DIPiDIP-10783N
IntActiP02359, 154 interactors
STRINGi316385.ECDH10B_3516

Structurei

Secondary structure

1179
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni16 – 18Combined sources3
Helixi21 – 30Combined sources10
Helixi32 – 34Combined sources3
Helixi36 – 54Combined sources19
Helixi59 – 69Combined sources11
Beta strandi72 – 76Combined sources5
Beta strandi78 – 80Combined sources3
Beta strandi81 – 83Combined sources3
Beta strandi87 – 90Combined sources4
Helixi93 – 109Combined sources17
Beta strandi113 – 115Combined sources3
Helixi116 – 128Combined sources13
Helixi133 – 144Combined sources12
Turni145 – 147Combined sources3
Helixi148 – 151Combined sources4

3D structure databases

ProteinModelPortaliP02359
SMRiP02359
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02359

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108UHY Bacteria
COG0049 LUCA
HOGENOMiHOG000039067
InParanoidiP02359
KOiK02992
OMAiTERLMNE
PhylomeDBiP02359

Family and domain databases

Gene3Di1.10.455.10, 1 hit
HAMAPiMF_00480_B Ribosomal_S7_B, 1 hit
InterProiView protein in InterPro
IPR000235 Ribosomal_S5/S7
IPR005717 Ribosomal_S7_bac/org-type
IPR020606 Ribosomal_S7_CS
IPR023798 Ribosomal_S7_dom
IPR036823 Ribosomal_S7_dom_sf
PANTHERiPTHR11205 PTHR11205, 1 hit
PfamiView protein in Pfam
PF00177 Ribosomal_S7, 1 hit
PIRSFiPIRSF002122 RPS7p_RPS7a_RPS5e_RPS7o, 1 hit
SUPFAMiSSF47973 SSF47973, 1 hit
TIGRFAMsiTIGR01029 rpsG_bact, 1 hit
PROSITEiView protein in PROSITE
PS00052 RIBOSOMAL_S7, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02359-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRRRVIGQR KILPDPKFGS ELLAKFVNIL MVDGKKSTAE SIVYSALETL
60 70 80 90 100
AQRSGKSELE AFEVALENVR PTVEVKSRRV GGSTYQVPVE VRPVRRNALA
110 120 130 140 150
MRWIVEAARK RGDKSMALRL ANELSDAAEN KGTAVKKRED VHRMAEANKA
160 170
FAHYRWLSLR SFSHQAGASS KQPALGYLN
Length:179
Mass (Da):20,019
Last modified:January 23, 2007 - v3
Checksum:i8627DB380C4A9C0D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti92Missing AA sequence (PubMed:385062).Curated1

Mass spectrometryi

Molecular mass is 19888.7 Da from positions 2 - 179. Determined by MALDI. 1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti157 – 179Missing in strain: B and L44. 1 PublicationAdd BLAST23

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA Translation: AAA58138.1
U00096 Genomic DNA Translation: AAC76366.1
AP009048 Genomic DNA Translation: BAE77950.1
V00355 Genomic DNA Translation: CAA23649.1
J01689 Genomic DNA Translation: AAA50990.1
X64592 Genomic DNA Translation: CAA45881.1
X65735 Genomic DNA Translation: CAA46644.1
J01688 Genomic DNA Translation: AAA50989.1
PIRiH65127 R3EC7K
RefSeqiNP_417800.1, NC_000913.3
WP_001138045.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76366; AAC76366; b3341
BAE77950; BAE77950; BAE77950
GeneIDi947846
KEGGiecj:JW3303
eco:b3341
PATRICifig|1411691.4.peg.3390

Similar proteinsi

Entry informationi

Entry nameiRS7_ECOLI
AccessioniPrimary (citable) accession number: P02359
Secondary accession number(s): Q2M706
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: June 20, 2018
This is version 200 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Ribosomal proteins
    Ribosomal proteins families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

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