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Protein

30S ribosomal protein S6

Gene

rpsF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds together with S18 to 16S ribosomal RNA.

GO - Molecular functioni

  • mRNA 5'-UTR binding Source: EcoCyc
  • small ribosomal subunit rRNA binding Source: EcoCyc
  • structural constituent of ribosome Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein, RNA-binding, rRNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10905-MONOMER
MetaCyc:EG10905-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
30S ribosomal protein S6
Alternative name(s):
Small ribosomal subunit protein bS61 Publication
Cleaved into the following 2 chains:
Gene namesi
Name:rpsF
Ordered Locus Names:b4200, JW4158
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10905 rpsF

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • cytosolic small ribosomal subunit Source: EcoliWiki

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000306401 – 13530S ribosomal protein S6, fully modified isoform1 PublicationAdd BLAST135
ChainiPRO_00000306411 – 13130S ribosomal protein S6, non-modified isoformAdd BLAST131

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei93N6-acetyllysine1 Publication1

Post-translational modificationi

5 different forms of the protein, varying only in the number of C-terminal glutamate residues, were isolated. The sequence shown is form S6-6, which is the longest. The first two Glu are encoded by the rpsF gene, the other Glu are added post-translationally by the RimK enzyme.1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP02358
PaxDbiP02358
PRIDEiP02358

2D gel databases

SWISS-2DPAGEiP02358

PTM databases

iPTMnetiP02358

Interactioni

Subunit structurei

Part of the 30S ribosomal subunit (PubMed:328274, PubMed:10094780, PubMed:12809609, PubMed:16272117, PubMed:27934701, PubMed:12244297, PubMed:27906160, PubMed:27906161, PubMed:28077875). Interacts weakly with L2 in one of the 3.5 A resolved structures (PubMed:16272117).9 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
rpsRP0A7T74EBI-543068,EBI-548844

Protein-protein interaction databases

BioGridi4259641, 276 interactors
DIPiDIP-10782N
IntActiP02358, 65 interactors
STRINGi316385.ECDH10B_4395

Structurei

Secondary structure

1135
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 4Combined sources3
Beta strandi7 – 10Combined sources4
Helixi13 – 17Combined sources5
Helixi18 – 31Combined sources14
Beta strandi35 – 37Combined sources3
Beta strandi40 – 45Combined sources6
Beta strandi51 – 53Combined sources3
Beta strandi57 – 61Combined sources5
Beta strandi64 – 66Combined sources3
Helixi70 – 80Combined sources11
Beta strandi82 – 88Combined sources7
Beta strandi89 – 91Combined sources3

3D structure databases

ProteinModelPortaliP02358
SMRiP02358
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02358

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108ZDX Bacteria
COG0360 LUCA
HOGENOMiHOG000040438
InParanoidiP02358
KOiK02990
OMAiNHYETVF
PhylomeDBiP02358

Family and domain databases

CDDicd00473 bS6, 1 hit
Gene3Di3.30.70.60, 1 hit
HAMAPiMF_00360 Ribosomal_S6, 1 hit
InterProiView protein in InterPro
IPR000529 Ribosomal_S6
IPR020815 Ribosomal_S6_CS
IPR020814 Ribosomal_S6_plastid/chlpt
IPR035980 Ribosomal_S6_sf
IPR014717 Transl_elong_EF1B/ribosomal_S6
PANTHERiPTHR21011 PTHR21011, 1 hit
PfamiView protein in Pfam
PF01250 Ribosomal_S6, 1 hit
SUPFAMiSSF54995 SSF54995, 1 hit
TIGRFAMsiTIGR00166 S6, 1 hit
PROSITEiView protein in PROSITE
PS01048 RIBOSOMAL_S6, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02358-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRHYEIVFMV HPDQSEQVPG MIERYTAAIT GAEGKIHRLE DWGRRQLAYP
60 70 80 90 100
INKLHKAHYV LMNVEAPQEV IDELETTFRF NDAVIRSMVM RTKHAVTEAS
110 120 130
PMVKAKDERR ERRDDFANET ADDAEAGDSE EEEEE
Length:135
Mass (Da):15,703
Last modified:July 21, 1986 - v1
Checksum:iF4CC629711C1FD0E
GO

Sequence cautioni

The sequence AAA97096 differs from that shown. Up to 4 Glu residues can be added post-translationally.1 Publication
The sequence AAC77157 differs from that shown. Up to 4 Glu residues can be added post-translationally.1 Publication
The sequence BAE78201 differs from that shown. Up to 4 Glu residues can be added post-translationally.1 Publication
The sequence CAA27652 differs from that shown. Up to 4 Glu residues can be added post-translationally.1 Publication

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti14Q → T AA sequence (PubMed:9868784).Curated1
Sequence conflicti20G → A AA sequence (PubMed:9868784).Curated1

Mass spectrometryi

Molecular mass is 15187.2 Da from positions 1 - 131. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04022 Genomic DNA Translation: CAA27652.1 Sequence problems.
U14003 Genomic DNA Translation: AAA97096.1 Sequence problems.
U00096 Genomic DNA Translation: AAC77157.5 Sequence problems.
AP009048 Genomic DNA Translation: BAE78201.1 Sequence problems.
PIRiC65231 R3EC6
RefSeqiNP_418621.5, NC_000913.3

Genome annotation databases

EnsemblBacteriaiAAC77157; AAC77157; b4200
BAE78201; BAE78201; BAE78201
GeneIDi948723
KEGGiecj:JW4158
eco:b4200
PATRICifig|511145.12.peg.4332

Similar proteinsi

Entry informationi

Entry nameiRS6_ECOLI
AccessioniPrimary (citable) accession number: P02358
Secondary accession number(s): Q2M6A5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: June 20, 2018
This is version 194 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Ribosomal proteins
    Ribosomal proteins families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

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