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Protein

T cell receptor beta variable 12-3

Gene

TRBV12-3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

V region of the variable domain of T cell receptor (TR) beta chain that participates in the antigen recognition (PubMed:24600447). Alpha-beta T cell receptors are antigen specific receptors which are essential to the immune response and are present on the cell surface of T lymphocytes. Recognize peptide-major histocompatibility (MH) (pMH) complexes that are displayed by antigen presenting cells (APC), a prerequisite for efficient T cell adaptive immunity against pathogens (PubMed:25493333). Binding of alpha-beta TR to pMH complex initiates TR-CD3 clustering on the cell surface and intracellular activation of LCK that phosphorylates the ITAM motifs of CD3G, CD3D, CD3E and CD247 enabling the recruitment of ZAP70. In turn ZAP70 phosphorylates LAT, which recruits numerous signaling molecules to form the LAT signalosome. The LAT signalosome propagates signal branching to three major signaling pathways, the calcium, the mitogen-activated protein kinase (MAPK) kinase and the nuclear factor NF-kappa-B (NF-kB) pathways, leading to the mobilization of transcription factors that are critical for gene expression and essential for T cell growth and differentiation (PubMed:23524462). The T cell repertoire is generated in the thymus, by V-(D)-J rearrangement. This repertoire is then shaped by intrathymic selection events to generate a peripheral T cell pool of self-MH restricted, non-autoaggressive T cells. Post-thymic interaction of alpha-beta TR with the pMH complexes shapes TR structural and functional avidity (PubMed:15040585).4 Publications

GO - Molecular functioni

  • MHC protein binding Source: UniProtKB
  • peptide antigen binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionReceptor
Biological processAdaptive immunity, Immunity

Enzyme and pathway databases

ReactomeiR-HSA-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell
R-HSA-202424 Downstream TCR signaling
R-HSA-202427 Phosphorylation of CD3 and TCR zeta chains
R-HSA-202430 Translocation of ZAP-70 to Immunological synapse
R-HSA-202433 Generation of second messenger molecules
R-HSA-389948 PD-1 signaling

Names & Taxonomyi

Protein namesi
Recommended name:
T cell receptor beta variable 12-31 Publication
Alternative name(s):
T-cell receptor beta chain V region YT351 Publication
Gene namesi
Name:TRBV12-31 Publication
Synonyms:TCRBV12S31 Publication, TCRBV8S11 Publication
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Unplaced

Organism-specific databases

EuPathDBiHostDB:ENSG00000274752.1
HGNCiHGNC:12185 TRBV12-3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000274752

Polymorphism and mutation databases

DMDMi136492

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_501401711922 – 115T cell receptor beta variable 12-3Sequence analysisAdd BLAST94

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi42 ↔ 111PROSITE-ProRule annotation
Glycosylationi90N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP01733
PeptideAtlasiP01733
PRIDEiP01733

PTM databases

iPTMnetiP01733
PhosphoSitePlusiP01733

Expressioni

Gene expression databases

BgeeiENSG00000274752 Expressed in 72 organ(s), highest expression level in adrenal gland

Interactioni

Subunit structurei

Alpha-beta TR is a heterodimer composed of an alpha and beta chain; disulfide-linked. The alpha-beta TR is associated with the transmembrane signaling CD3 coreceptor proteins to form the TR-CD3 (TcR or TCR). The assembly of alpha-beta TR heterodimers with CD3 occurs in the endoplasmic reticulum where a single alpha-beta TR heterodimer associates with one CD3D-CD3E heterodimer, one CD3G-CD3E heterodimer and one CD247 homodimer forming a stable octomeric structure. CD3D-CD3E and CD3G-CD3E heterodimers preferentially associate with TR alpha and TR beta chains, respectively. The association of the CD247 homodimer is the last step of TcR assembly in the endoplasmic reticulum and is required for transport to the cell surface.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

IntActiP01733, 2 interactors
MINTiP01733

Structurei

3D structure databases

ProteinModelPortaliP01733
SMRiP01733
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – ›115Ig-likePROSITE-ProRule annotationAdd BLAST›94

Keywords - Domaini

Immunoglobulin domain, Signal

Phylogenomic databases

GeneTreeiENSGT00910000144127
HOVERGENiHBG100543
InParanoidiP01733
OMAiSAVFWYR
PhylomeDBiP01733

Family and domain databases

Gene3Di2.60.40.10, 1 hit
InterProiView protein in InterPro
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR013106 Ig_V-set
PfamiView protein in Pfam
PF07686 V-set, 1 hit
SMARTiView protein in SMART
SM00406 IGv, 1 hit
SUPFAMiSSF48726 SSF48726, 1 hit
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01733-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDSWTFCCVS LCILVAKHTD AGVIQSPRHE VTEMGQEVTL RCKPISGHNS
60 70 80 90 100
LFWYRQTMMR GLELLIYFNN NVPIDDSGMP EDRFSAKMPN ASFSTLKIQP
110
SEPRDSAVYF CASSL
Length:115
Mass (Da):12,997
Last modified:July 18, 2018 - v2
Checksum:iD9BF12E944882F14
GO

Sequence cautioni

The sequence AAA60669 differs from that shown. Sequence from TRBJ1-2 gene product.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti115L → F (PubMed:6336315).Curated1
Sequence conflicti115L → LA in AAC13336 (PubMed:8650574).Curated1
Non-terminal residuei1151

Polymorphismi

There are several alleles. The sequence shown is that of IMGT allele TRBV12-3*01.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14158 Genomic DNA Translation: AAA60669.1 Sequence problems.
L36092 Genomic DNA Translation: AAC13336.1
AC244196 Genomic DNA No translation available.
PIRiA02000 RWHUVY
S03488
S03510

Genome annotation databases

EnsembliENST00000620569; ENSP00000477916; ENSG00000274752
ENST00000633292; ENSP00000487964; ENSG00000282208

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14158 Genomic DNA Translation: AAA60669.1 Sequence problems.
L36092 Genomic DNA Translation: AAC13336.1
AC244196 Genomic DNA No translation available.
PIRiA02000 RWHUVY
S03488
S03510

3D structure databases

ProteinModelPortaliP01733
SMRiP01733
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP01733, 2 interactors
MINTiP01733

PTM databases

iPTMnetiP01733
PhosphoSitePlusiP01733

Polymorphism and mutation databases

DMDMi136492

Proteomic databases

MaxQBiP01733
PeptideAtlasiP01733
PRIDEiP01733

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000620569; ENSP00000477916; ENSG00000274752
ENST00000633292; ENSP00000487964; ENSG00000282208

Organism-specific databases

EuPathDBiHostDB:ENSG00000274752.1
GeneCardsiTRBV12-3
HGNCiHGNC:12185 TRBV12-3
OpenTargetsiENSG00000274752
GenAtlasiSearch...

Phylogenomic databases

GeneTreeiENSGT00910000144127
HOVERGENiHBG100543
InParanoidiP01733
OMAiSAVFWYR
PhylomeDBiP01733

Enzyme and pathway databases

ReactomeiR-HSA-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell
R-HSA-202424 Downstream TCR signaling
R-HSA-202427 Phosphorylation of CD3 and TCR zeta chains
R-HSA-202430 Translocation of ZAP-70 to Immunological synapse
R-HSA-202433 Generation of second messenger molecules
R-HSA-389948 PD-1 signaling

Miscellaneous databases

PROiPR:P01733

Gene expression databases

BgeeiENSG00000274752 Expressed in 72 organ(s), highest expression level in adrenal gland

Family and domain databases

Gene3Di2.60.40.10, 1 hit
InterProiView protein in InterPro
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR013106 Ig_V-set
PfamiView protein in Pfam
PF07686 V-set, 1 hit
SMARTiView protein in SMART
SM00406 IGv, 1 hit
SUPFAMiSSF48726 SSF48726, 1 hit
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTVBL3_HUMAN
AccessioniPrimary (citable) accession number: P01733
Secondary accession number(s): A0A0A6YYD5, A0A5A7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 18, 2018
Last modified: September 12, 2018
This is version 127 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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