UniProtKB - P01731 (CD8A_MOUSE)
T-cell surface glycoprotein CD8 alpha chain
Cd8a
Functioni
GO - Molecular functioni
- identical protein binding Source: MGI
- MHC class I protein complex binding Source: MGI
- protein kinase binding Source: MGI
GO - Biological processi
- cell surface receptor signaling pathway Source: MGI
- cytotoxic T cell differentiation Source: MGI
- defense response to virus Source: MGI
- positive regulation of calcium-mediated signaling Source: MGI
- T cell activation Source: MGI
- T cell mediated immunity Source: MGI
Keywordsi
Biological process | Adaptive immunity, Immunity |
Enzyme and pathway databases
Reactomei | R-MMU-198933, Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell |
Names & Taxonomyi
Protein namesi | Recommended name: T-cell surface glycoprotein CD8 alpha chainAlternative name(s): T-cell surface glycoprotein Lyt-2 CD_antigen: CD8a |
Gene namesi | Name:Cd8a Synonyms:Lyt-2, Lyt2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:88346, Cd8a |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Single-pass type I membrane protein By similarity
Note: Cd8a localizes to lipid rafts only when associated with its partner Cd8b.By similarity
Plasma Membrane
- external side of plasma membrane Source: MGI
- plasma membrane Source: MGI
- plasma membrane raft Source: MGI
Other locations
- cell surface Source: MGI
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 28 – 196 | ExtracellularSequence analysisAdd BLAST | 169 | |
Transmembranei | 197 – 217 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 218 – 247 | CytoplasmicSequence analysisAdd BLAST | 30 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 27 | Add BLAST | 27 | |
ChainiPRO_0000014639 | 28 – 247 | T-cell surface glycoprotein CD8 alpha chainAdd BLAST | 220 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 53 ↔ 129 | PROSITE-ProRule annotation1 Publication | ||
Glycosylationi | 69 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 97 | N-linked (GlcNAc...) asparagine | 1 | |
Glycosylationi | 150 | N-linked (GlcNAc...) asparagine | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, Lipoprotein, PalmitateProteomic databases
EPDi | P01731 |
MaxQBi | P01731 |
PaxDbi | P01731 |
PRIDEi | P01731 |
PTM databases
GlyGeni | P01731, 3 sites |
iPTMneti | P01731 |
PhosphoSitePlusi | P01731 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000053977, Expressed in thymus and 134 other tissues |
ExpressionAtlasi | P01731, baseline and differential |
Genevisiblei | P01731, MM |
Interactioni
Subunit structurei
Forms disulfide-linked heterodimers with CD8B at the cell surface.
Forms also homodimers in several cell types including NK-cells or peripheral blood T-lymphocytes.
Interacts with the MHC class I HLA-A/B2M dimer.
Interacts with LCK in a zinc-dependent manner.
By similarity2 PublicationsBinary interactionsi
P01731
With | #Exp. | IntAct |
---|---|---|
Lck [P06240] | 2 | EBI-1433,EBI-1401 |
GO - Molecular functioni
- identical protein binding Source: MGI
- protein kinase binding Source: MGI
Protein-protein interaction databases
IntActi | P01731, 4 interactors |
MINTi | P01731 |
STRINGi | 10090.ENSMUSP00000068123 |
Miscellaneous databases
RNActi | P01731, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P01731 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P01731 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 28 – 139 | Ig-like V-typeAdd BLAST | 112 |
Keywords - Domaini
Immunoglobulin domain, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502SAZN, Eukaryota |
GeneTreei | ENSGT00940000156588 |
HOGENOMi | CLU_085753_0_0_1 |
InParanoidi | P01731 |
OMAi | YYFCSVV |
OrthoDBi | 1448152at2759 |
PhylomeDBi | P01731 |
TreeFami | TF336070 |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR015468, CD8_asu IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003599, Ig_sub IPR013106, Ig_V-set |
PANTHERi | PTHR10441, PTHR10441, 1 hit |
Pfami | View protein in Pfam PF07686, V-set, 1 hit |
SMARTi | View protein in SMART SM00409, IG, 1 hit SM00406, IGv, 1 hit |
SUPFAMi | SSF48726, SSF48726, 1 hit |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 1 produced by isoform ialternative splicing. AlignAdd to basketThis entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MASPLTRFLS LNLLLLGESI ILGSGEAKPQ APELRIFPKK MDAELGQKVD
60 70 80 90 100
LVCEVLGSVS QGCSWLFQNS SSKLPQPTFV VYMASSHNKI TWDEKLNSSK
110 120 130 140 150
LFSAMRDTNN KYVLTLNKFS KENEGYYFCS VISNSVMYFS SVVPVLQKVN
160 170 180 190 200
STTTKPVLRT PSPVHPTGTS QPQRPEDCRP RGSVKGTGLD FACDIYIWAP
210 220 230 240
LAGICVALLL SLIITLICYH RSRKRVCKCP RPLVRQEGKP RPSEKIV
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ8CAX3 | Q8CAX3_MOUSE | T-cell surface glycoprotein CD8 alp... | Cd8a | 222 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 81 | Missing in AAA39477 (PubMed:3495785).Curated | 1 | |
Sequence conflicti | 81 | Missing in CAA68352 (PubMed:3495785).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 105 | M → V in strain: C.AKR. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M12825 mRNA Translation: AAA39476.1 M16981 mRNA Translation: AAA39477.1 Different termination. M12052 mRNA Translation: AAA39478.1 Y00157 Genomic DNA Translation: CAA68352.2 M22064 Genomic DNA Translation: AAA39665.1 M12977 , M12819, M12975, M12976 Genomic DNA Translation: AAA39475.1 |
CCDSi | CCDS39507.1 [P01731-1] |
PIRi | A01998, RWMST2 A29523 A34954 |
RefSeqi | NP_001074579.1, NM_001081110.2 [P01731-1] |
Genome annotation databases
Ensembli | ENSMUST00000066747; ENSMUSP00000068123; ENSMUSG00000053977 [P01731-1] |
GeneIDi | 12525 |
KEGGi | mmu:12525 |
UCSCi | uc009cgr.1, mouse [P01731-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M12825 mRNA Translation: AAA39476.1 M16981 mRNA Translation: AAA39477.1 Different termination. M12052 mRNA Translation: AAA39478.1 Y00157 Genomic DNA Translation: CAA68352.2 M22064 Genomic DNA Translation: AAA39665.1 M12977 , M12819, M12975, M12976 Genomic DNA Translation: AAA39475.1 |
CCDSi | CCDS39507.1 [P01731-1] |
PIRi | A01998, RWMST2 A29523 A34954 |
RefSeqi | NP_001074579.1, NM_001081110.2 [P01731-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1BQH | X-ray | 2.80 | G/H/I/K | 28-156 | [»] | |
1NEZ | X-ray | 2.10 | G/H | 28-149 | [»] | |
2ARJ | X-ray | 2.88 | Q/R | 28-150 | [»] | |
2ATP | X-ray | 2.40 | A/C | 28-149 | [»] | |
3B9K | X-ray | 2.70 | A/E | 28-151 | [»] | |
3DMM | X-ray | 2.60 | C | 23-188 | [»] | |
SMRi | P01731 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P01731, 4 interactors |
MINTi | P01731 |
STRINGi | 10090.ENSMUSP00000068123 |
PTM databases
GlyGeni | P01731, 3 sites |
iPTMneti | P01731 |
PhosphoSitePlusi | P01731 |
Proteomic databases
EPDi | P01731 |
MaxQBi | P01731 |
PaxDbi | P01731 |
PRIDEi | P01731 |
Protocols and materials databases
ABCDi | P01731, 11 sequenced antibodies |
Antibodypediai | 3495, 5131 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000066747; ENSMUSP00000068123; ENSMUSG00000053977 [P01731-1] |
GeneIDi | 12525 |
KEGGi | mmu:12525 |
UCSCi | uc009cgr.1, mouse [P01731-1] |
Organism-specific databases
CTDi | 925 |
MGIi | MGI:88346, Cd8a |
Phylogenomic databases
eggNOGi | ENOG502SAZN, Eukaryota |
GeneTreei | ENSGT00940000156588 |
HOGENOMi | CLU_085753_0_0_1 |
InParanoidi | P01731 |
OMAi | YYFCSVV |
OrthoDBi | 1448152at2759 |
PhylomeDBi | P01731 |
TreeFami | TF336070 |
Enzyme and pathway databases
Reactomei | R-MMU-198933, Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell |
Miscellaneous databases
BioGRID-ORCSi | 12525, 0 hits in 19 CRISPR screens |
ChiTaRSi | Cd8a, mouse |
EvolutionaryTracei | P01731 |
PROi | PR:P01731 |
RNActi | P01731, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000053977, Expressed in thymus and 134 other tissues |
ExpressionAtlasi | P01731, baseline and differential |
Genevisiblei | P01731, MM |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR015468, CD8_asu IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003599, Ig_sub IPR013106, Ig_V-set |
PANTHERi | PTHR10441, PTHR10441, 1 hit |
Pfami | View protein in Pfam PF07686, V-set, 1 hit |
SMARTi | View protein in SMART SM00409, IG, 1 hit SM00406, IGv, 1 hit |
SUPFAMi | SSF48726, SSF48726, 1 hit |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CD8A_MOUSE | |
Accessioni | P01731Primary (citable) accession number: P01731 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | December 2, 2020 | |
This is version 190 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references