UniProtKB - P01619 (KV320_HUMAN)
Protein
Immunoglobulin kappa variable 3-20
Gene
IGKV3-20
Organism
Homo sapiens (Human)
Status
Functioni
V region of the variable domain of immunoglobulin light chains that participates in the antigen recognition (PubMed:24600447). Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:20176268, PubMed:17576170).4 Publications
Caution
For an example of a full-length immunoglobulin kappa light chain see AC P0DOX7.Curated
GO - Molecular functioni
- antigen binding Source: UniProtKB
GO - Biological processi
- antibacterial humoral response Source: UniProtKB
- complement activation Source: Reactome
- complement activation, classical pathway Source: Reactome
- Fc-epsilon receptor signaling pathway Source: Reactome
- Fc-gamma receptor signaling pathway involved in phagocytosis Source: Reactome
- glomerular filtration Source: UniProtKB
- immune response Source: GO_Central
- immunoglobulin production Source: GO_Central
- leukocyte migration Source: Reactome
- receptor-mediated endocytosis Source: Reactome
- regulation of complement activation Source: Reactome
- regulation of immune response Source: Reactome
Keywordsi
Biological process | Adaptive immunity, Immunity |
Enzyme and pathway databases
PathwayCommonsi | P01619 |
Reactomei | R-HSA-166663, Initial triggering of complement R-HSA-173623, Classical antibody-mediated complement activation R-HSA-198933, Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell R-HSA-202733, Cell surface interactions at the vascular wall R-HSA-2029481, FCGR activation R-HSA-2029482, Regulation of actin dynamics for phagocytic cup formation R-HSA-2029485, Role of phospholipids in phagocytosis R-HSA-2168880, Scavenging of heme from plasma R-HSA-2454202, Fc epsilon receptor (FCERI) signaling R-HSA-2730905, Role of LAT2/NTAL/LAB on calcium mobilization R-HSA-2871796, FCERI mediated MAPK activation R-HSA-2871809, FCERI mediated Ca+2 mobilization R-HSA-2871837, FCERI mediated NF-kB activation R-HSA-5690714, CD22 mediated BCR regulation R-HSA-9664323, FCGR3A-mediated IL10 synthesis R-HSA-9664422, FCGR3A-mediated phagocytosis R-HSA-977606, Regulation of Complement cascade R-HSA-983695, Antigen activates B Cell Receptor (BCR) leading to generation of second messengers |
Protein family/group databases
IMGT/GENE-DB | IGKV3-20 |
Names & Taxonomyi
Protein namesi | Recommended name: Immunoglobulin kappa variable 3-202 PublicationsAlternative name(s): Ig kappa chain V-III region B61 Publication Ig kappa chain V-III region GOL1 Publication Ig kappa chain V-III region HAH1 Publication Ig kappa chain V-III region HIC1 Publication Ig kappa chain V-III region IARC/BL411 Publication Ig kappa chain V-III region NG91 Publication Ig kappa chain V-III region SIE1 Publication Ig kappa chain V-III region Ti1 Publication Ig kappa chain V-III region WOL1 Publication |
Gene namesi | Name:IGKV3-202 Publications |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000239951.1 |
HGNCi | HGNC:5817, IGKV3-20 |
neXtProti | NX_P01619 |
Subcellular locationi
Plasma membrane
- Cell membrane 2 Publications
Extracellular region or secreted
- Secreted 2 Publications
Extracellular region or secreted
- blood microparticle Source: UniProtKB
- extracellular exosome Source: UniProtKB
- extracellular region Source: Reactome
- extracellular space Source: UniProtKB
- monomeric IgA immunoglobulin complex Source: UniProtKB
- pentameric IgM immunoglobulin complex Source: UniProtKB
- secretory IgA immunoglobulin complex Source: UniProtKB
Plasma Membrane
- plasma membrane Source: Reactome
Keywords - Cellular componenti
Cell membrane, Immunoglobulin, Membrane, SecretedPathology & Biotechi
Organism-specific databases
OpenTargetsi | ENSG00000239951 |
Miscellaneous databases
Pharosi | P01619, Tbio |
Chemistry databases
DrugBanki | DB09130, Copper DB01593, Zinc DB14487, Zinc acetate |
Polymorphism and mutation databases
BioMutai | IGKV3-20 |
DMDMi | 125801 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | 4 PublicationsAdd BLAST | 20 | |
ChainiPRO_0000059762 | 21 – 116 | Immunoglobulin kappa variable 3-204 PublicationsAdd BLAST | 96 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 43 ↔ 109 | PROSITE-ProRule annotation |
Keywords - PTMi
Disulfide bondProteomic databases
jPOSTi | P01619 |
MassIVEi | P01619 |
PeptideAtlasi | P01619 |
PRIDEi | P01619 |
ProteomicsDBi | 51420 51421 51422 51423 51424 51678 51885 53550 53551 |
TopDownProteomicsi | P01619 |
Expressioni
Gene expression databases
Bgeei | ENSG00000239951, Expressed in tonsil and 151 other tissues |
Interactioni
Subunit structurei
Immunoglobulins are composed of two identical heavy chains and two identical light chains; disulfide-linked.
1 PublicationProtein-protein interaction databases
IntActi | P01619, 3 interactors |
MINTi | P01619 |
Miscellaneous databases
RNActi | P01619, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P01619 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 21 – ›116 | Ig-likePROSITE-ProRule annotationAdd BLAST | ›96 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 21 – 43 | Framework-11 PublicationAdd BLAST | 23 | |
Regioni | 44 – 55 | Complementarity-determining-11 PublicationAdd BLAST | 12 | |
Regioni | 56 – 70 | Framework-21 PublicationAdd BLAST | 15 | |
Regioni | 71 – 77 | Complementarity-determining-21 Publication | 7 | |
Regioni | 78 – 109 | Framework-31 PublicationAdd BLAST | 32 | |
Regioni | 110 – ›116 | Complementarity-determining-31 Publication | ›7 |
Keywords - Domaini
Immunoglobulin domain, SignalPhylogenomic databases
GeneTreei | ENSGT00940000154413 |
OMAi | SASRCDI |
PhylomeDBi | P01619 |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003599, Ig_sub IPR013106, Ig_V-set |
Pfami | View protein in Pfam PF07686, V-set, 1 hit |
SMARTi | View protein in SMART SM00409, IG, 1 hit SM00406, IGv, 1 hit |
SUPFAMi | SSF48726, SSF48726, 1 hit |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P01619-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
METPAQLLFL LLLWLPDTTG EIVLTQSPGT LSLSPGERAT LSCRASQSVS
60 70 80 90 100
SSYLAWYQQK PGQAPRLLIY GASSRATGIP DRFSGSGSGT DFTLTISRLE
110
PEDFAVYYCQ QYGSSP
Sequence cautioni
The sequence CAA77316 differs from that shown. Chimeric DNA. A chimeric DNA corresponding to regions V and J of immunoglobulin kappa light chain.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 17 – 19 | DTT → VPS (PubMed:6419127).Curated | 3 | |
Sequence conflicti | 38 | R → S in CAA77316 (PubMed:2997711).Curated | 1 | |
Sequence conflicti | 40 | T → A AA sequence (PubMed:11946339).Curated | 1 | |
Sequence conflicti | 46 – 52 | SQSVSSS → ALLSSRG AA sequence (PubMed:3086710).Curated | 7 | |
Sequence conflicti | 49 – 52 | VSSS → LSGN AA sequence (PubMed:11946339).Curated | 4 | |
Sequence conflicti | 51 | S → N AA sequence (PubMed:6794615).Curated | 1 | |
Sequence conflicti | 51 | S → N AA sequence (PubMed:5027703).Curated | 1 | |
Sequence conflicti | 52 – 53 | SY → N in CAA77316 (PubMed:2997711).Curated | 2 | |
Sequence conflicti | 52 | S → G AA sequence (PubMed:6794615).Curated | 1 | |
Sequence conflicti | 53 | Y → F AA sequence (PubMed:5027703).Curated | 1 | |
Sequence conflicti | 55 | A → G AA sequence (PubMed:6794615).Curated | 1 | |
Sequence conflicti | 61 | P → R in CAA77316 (PubMed:2997711).Curated | 1 | |
Sequence conflicti | 64 | A → S in CAA77316 (PubMed:2997711).Curated | 1 | |
Sequence conflicti | 69 | I → M AA sequence (PubMed:11946339).Curated | 1 | |
Sequence conflicti | 69 | I → M AA sequence (PubMed:3086710).Curated | 1 | |
Sequence conflicti | 70 – 71 | YG → RD in CAA77316 (PubMed:2997711).Curated | 2 | |
Sequence conflicti | 71 | G → V AA sequence (PubMed:5027703).Curated | 1 | |
Sequence conflicti | 72 | A → V AA sequence (PubMed:11946339).Curated | 1 | |
Sequence conflicti | 73 | S → T (PubMed:6419127).Curated | 1 | |
Sequence conflicti | 77 | T → N in CAA77316 (PubMed:2997711).Curated | 1 | |
Sequence conflicti | 87 | G → A (PubMed:6419127).Curated | 1 | |
Sequence conflicti | 90 | T → A AA sequence (PubMed:11946339).Curated | 1 | |
Sequence conflicti | 95 | T → I in CAA77316 (PubMed:2997711).Curated | 1 | |
Sequence conflicti | 102 | E → D AA sequence (PubMed:6794615).Curated | 1 | |
Sequence conflicti | 113 | G → S in CAA77316 (PubMed:2997711).Curated | 1 | |
Sequence conflicti | 114 – 116 | SSP → NSQ (PubMed:6419127).Curated | 3 | |
Sequence conflicti | 114 | S → T (PubMed:3127527).Curated | 1 | |
Sequence conflicti | 114 | S → T in CAA77316 (PubMed:2997711).Curated | 1 | |
Sequence conflicti | 115 – 116 | SP → LG AA sequence (PubMed:6794615).Curated | 2 | |
Non-terminal residuei | 116 | 1 |
Polymorphismi
There are several alleles. The sequence shown is that of IMGT allele IGKV3-20*01.
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z00021 Genomic DNA Translation: CAA77316.1 Sequence problems. AC245015 Genomic DNA No translation available. |
PIRi | A01891, K3HUB6 A01892, K3HUSI A01893, K3HUGO A01894, K3HUNG A01895, K3HUTI A01896, K3HUWL A01899, K3HU41 C27594 E30607 PL0021, K3HUHI PL0022, K3HUHA |
Genome annotation databases
Ensembli | ENST00000492167; ENSP00000418649; ENSG00000239951 ENST00000632822; ENSP00000487628; ENSG00000282402 |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z00021 Genomic DNA Translation: CAA77316.1 Sequence problems. AC245015 Genomic DNA No translation available. |
PIRi | A01891, K3HUB6 A01892, K3HUSI A01893, K3HUGO A01894, K3HUNG A01895, K3HUTI A01896, K3HUWL A01899, K3HU41 C27594 E30607 PL0021, K3HUHI PL0022, K3HUHA |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1DH5 | model | - | L | 21-116 | [»] | |
4LRN | X-ray | 1.89 | L | 21-116 | [»] | |
4M62 | X-ray | 1.80 | L/M | 21-116 | [»] | |
4M8Q | X-ray | 2.89 | B/L | 21-116 | [»] | |
4OB5 | X-ray | 1.70 | L | 21-116 | [»] | |
4ODX | X-ray | 3.10 | B/L | 21-116 | [»] | |
SMRi | P01619 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P01619, 3 interactors |
MINTi | P01619 |
Chemistry databases
DrugBanki | DB09130, Copper DB01593, Zinc DB14487, Zinc acetate |
Protein family/group databases
IMGT/GENE-DB | IGKV3-20 |
Polymorphism and mutation databases
BioMutai | IGKV3-20 |
DMDMi | 125801 |
Proteomic databases
jPOSTi | P01619 |
MassIVEi | P01619 |
PeptideAtlasi | P01619 |
PRIDEi | P01619 |
ProteomicsDBi | 51420 51421 51422 51423 51424 51678 51885 53550 53551 |
TopDownProteomicsi | P01619 |
Genome annotation databases
Ensembli | ENST00000492167; ENSP00000418649; ENSG00000239951 ENST00000632822; ENSP00000487628; ENSG00000282402 |
Organism-specific databases
EuPathDBi | HostDB:ENSG00000239951.1 |
GeneCardsi | IGKV3-20 |
HGNCi | HGNC:5817, IGKV3-20 |
neXtProti | NX_P01619 |
OpenTargetsi | ENSG00000239951 |
GenAtlasi | Search... |
Phylogenomic databases
GeneTreei | ENSGT00940000154413 |
OMAi | SASRCDI |
PhylomeDBi | P01619 |
Enzyme and pathway databases
PathwayCommonsi | P01619 |
Reactomei | R-HSA-166663, Initial triggering of complement R-HSA-173623, Classical antibody-mediated complement activation R-HSA-198933, Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell R-HSA-202733, Cell surface interactions at the vascular wall R-HSA-2029481, FCGR activation R-HSA-2029482, Regulation of actin dynamics for phagocytic cup formation R-HSA-2029485, Role of phospholipids in phagocytosis R-HSA-2168880, Scavenging of heme from plasma R-HSA-2454202, Fc epsilon receptor (FCERI) signaling R-HSA-2730905, Role of LAT2/NTAL/LAB on calcium mobilization R-HSA-2871796, FCERI mediated MAPK activation R-HSA-2871809, FCERI mediated Ca+2 mobilization R-HSA-2871837, FCERI mediated NF-kB activation R-HSA-5690714, CD22 mediated BCR regulation R-HSA-9664323, FCGR3A-mediated IL10 synthesis R-HSA-9664422, FCGR3A-mediated phagocytosis R-HSA-977606, Regulation of Complement cascade R-HSA-983695, Antigen activates B Cell Receptor (BCR) leading to generation of second messengers |
Miscellaneous databases
Pharosi | P01619, Tbio |
PROi | PR:P01619 |
RNActi | P01619, protein |
Gene expression databases
Bgeei | ENSG00000239951, Expressed in tonsil and 151 other tissues |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003599, Ig_sub IPR013106, Ig_V-set |
Pfami | View protein in Pfam PF07686, V-set, 1 hit |
SMARTi | View protein in SMART SM00409, IG, 1 hit SM00406, IGv, 1 hit |
SUPFAMi | SSF48726, SSF48726, 1 hit |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KV320_HUMAN | |
Accessioni | P01619Primary (citable) accession number: P01619 Secondary accession number(s): A0A0B4J1Z6 P18136 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | November 2, 2016 | |
Last modified: | December 2, 2020 | |
This is version 117 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM