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Protein

Cholera enterotoxin subunit B

Gene

ctxB

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself.

GO - Molecular functioni

  • galactose binding Source: CAFA
  • host cell surface binding Source: TIGR
  • toxin activity Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionEnterotoxin, Toxin

Enzyme and pathway databases

BioCyciMetaCyc:VC1456-MONOMER
VCHO:VC1456-MONOMER

Protein family/group databases

UniLectiniP01556

Names & Taxonomyi

Protein namesi
Recommended name:
Cholera enterotoxin subunit B
Alternative name(s):
Cholera enterotoxin B chain
Cholera enterotoxin gamma chain
Choleragenoid
Gene namesi
Name:ctxB
Synonyms:toxB
Ordered Locus Names:VC_1456
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 1

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi78H → A: Loss of toxicity. 1

Chemistry databases

DrugBankiDB02802 5-Aminocarbonyl-3-Nitrophenyl-Alpha-D-Galactopyranose
DB02379 Beta-D-Glucose
DB02474 Bmsc-0013
DB04210 BV1
DB02574 BV2
DB02903 BV3
DB02572 BV4
DB02213 Metanitrophenyl-Alpha-D-Galactoside
DB03721 N-acetyl-alpha-neuraminic acid
DB03235 N-{3-[4-(3-Amino-Propyl)-Piperazin-1-Yl]-Propyl}-3-Nitro-5-(Galactopyranosyl)-Alpha-Benzamide
DB04073 N-{3-[4-(3-Amino-Propyl)-Piperazin-1-Yl]-Propyl}-3-Nitro-5-(Galactopyranosyl)-Beta-Benzamide
DB03754 Tris

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 212 PublicationsAdd BLAST21
ChainiPRO_000001934422 – 124Cholera enterotoxin subunit BAdd BLAST103

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi30 ↔ 1071 Publication

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

The holotoxin (choleragen) consists of a pentameric ring of B subunits whose central pore is occupied by the A subunit. The A subunit contains two chains, A1 and A2, linked by a disulfide bridge.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ctxAP015555EBI-1038383,EBI-1038392

Protein-protein interaction databases

DIPiDIP-6256N
IntActiP01556, 5 interactors
MINTiP01556
STRINGi243277.VC1456

Structurei

Secondary structure

1124
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP01556
SMRiP01556
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP01556

Family & Domainsi

Keywords - Domaini

Signal

Phylogenomic databases

KOiK10929
OMAiHIDSQKK

Family and domain databases

InterProiView protein in InterPro
IPR008992 Enterotoxin
IPR001835 Enterotoxin_B
PfamiView protein in Pfam
PF01376 Enterotoxin_b, 1 hit
PRINTSiPR00772 ENTEROTOXINB
ProDomiView protein in ProDom or Entries sharing at least one domain
PD012805 Enterotoxin_B, 1 hit
SUPFAMiSSF50203 SSF50203, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01556-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MIKLKFGVFF TVLLSSAYAH GTPQNITDLC AEYHNTQIYT LNDKIFSYTE
60 70 80 90 100
SLAGKREMAI ITFKNGAIFQ VEVPGSQHID SQKKAIERMK DTLRIAYLTE
110 120
AKVEKLCVWN NKTPHAIAAI SMAN
Length:124
Mass (Da):13,957
Last modified:August 13, 1987 - v1
Checksum:i9AA393E3EA8E3EBF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti33Y → S in CAA24996 (PubMed:6646234).Curated1
Sequence conflicti39Y → H AA sequence (PubMed:903363).Curated1
Sequence conflicti39Y → H AA sequence (PubMed:903362).Curated1
Sequence conflicti43D → N AA sequence (PubMed:903363).Curated1
Sequence conflicti43D → N AA sequence (PubMed:903362).Curated1
Sequence conflicti68I → T AA sequence (PubMed:903363).Curated1
Sequence conflicti68I → T AA sequence (PubMed:903362).Curated1
Sequence conflicti70Q → E AA sequence (PubMed:903362).Curated1
Sequence conflicti75G → S in CAA24996 (PubMed:6646234).Curated1
Sequence conflicti91D → N AA sequence (PubMed:903363).Curated1
Sequence conflicti91D → N AA sequence (PubMed:903362).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01170 Genomic DNA Translation: AAA27573.1
X00171 Genomic DNA Translation: CAA24996.1
X76390 Genomic DNA Translation: CAA53973.1
X76391 Genomic DNA Translation: CAA53976.1
X58786 Genomic DNA Translation: CAA41593.1
D30053 Genomic DNA Translation: BAA06291.1
AE003852 Genomic DNA Translation: AAF94613.1
PIRiS14624 XVVCB
RefSeqiNP_231099.1, NC_002505.1
WP_000593522.1, NC_002505.1

Genome annotation databases

EnsemblBacteriaiAAF94613; AAF94613; VC_1456
GeneIDi2613962
KEGGivch:VC1456
PATRICifig|243277.26.peg.1386

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01170 Genomic DNA Translation: AAA27573.1
X00171 Genomic DNA Translation: CAA24996.1
X76390 Genomic DNA Translation: CAA53973.1
X76391 Genomic DNA Translation: CAA53976.1
X58786 Genomic DNA Translation: CAA41593.1
D30053 Genomic DNA Translation: BAA06291.1
AE003852 Genomic DNA Translation: AAF94613.1
PIRiS14624 XVVCB
RefSeqiNP_231099.1, NC_002505.1
WP_000593522.1, NC_002505.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CHPX-ray2.00D/E/F/G/H22-124[»]
1CHQX-ray2.10D/E/F/G/H22-124[»]
1CT1X-ray2.30D/E/F/G/H22-124[»]
1FGBX-ray2.40D/E/F/G/H22-124[»]
1G8ZX-ray2.00D/E/F/G/H22-124[»]
1JR0X-ray1.30D/E/F/G/H22-124[»]
1MD2X-ray1.45D/E/F/G/H22-124[»]
1RCVX-ray1.60D/E/F/G/H22-124[»]
1RD9X-ray1.44D/E/F/G/H22-124[»]
1RDPX-ray1.35D/E/F/G/H22-124[»]
1RF2X-ray1.35D/E/F/G/H22-124[»]
1S5BX-ray2.13D/E/F/G/H22-124[»]
1S5CX-ray2.50D/E/F/G/H22-124[»]
1S5DX-ray1.75D/E/F/G/H22-124[»]
1S5EX-ray1.90D/E/F/G/H/J/K/L/M/N22-124[»]
1S5FX-ray2.60D/E/F/G/H22-124[»]
1XTCX-ray2.40D/E/F/G/H22-124[»]
2CHBX-ray2.00D/E/F/G/H22-124[»]
3CHBX-ray1.25D/E/F/G/H22-124[»]
3EFXX-ray1.94D/E/F/G/H/I/J/K/L/M23-124[»]
5ELCX-ray1.50A/B/C/D/E/F/G/H/I/J22-124[»]
5ELEX-ray1.60A/B/C/D/E/F/G/H/I/J22-124[»]
5ELFX-ray1.55A/B/C/D/E/F/G/H/I/J22-124[»]
5LZGX-ray1.13A/B/C/D/E22-124[»]
5LZJX-ray1.20A/B/C/D/E22-124[»]
ProteinModelPortaliP01556
SMRiP01556
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-6256N
IntActiP01556, 5 interactors
MINTiP01556
STRINGi243277.VC1456

Chemistry databases

DrugBankiDB02802 5-Aminocarbonyl-3-Nitrophenyl-Alpha-D-Galactopyranose
DB02379 Beta-D-Glucose
DB02474 Bmsc-0013
DB04210 BV1
DB02574 BV2
DB02903 BV3
DB02572 BV4
DB02213 Metanitrophenyl-Alpha-D-Galactoside
DB03721 N-acetyl-alpha-neuraminic acid
DB03235 N-{3-[4-(3-Amino-Propyl)-Piperazin-1-Yl]-Propyl}-3-Nitro-5-(Galactopyranosyl)-Alpha-Benzamide
DB04073 N-{3-[4-(3-Amino-Propyl)-Piperazin-1-Yl]-Propyl}-3-Nitro-5-(Galactopyranosyl)-Beta-Benzamide
DB03754 Tris

Protein family/group databases

UniLectiniP01556

Protocols and materials databases

DNASUi2613962
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF94613; AAF94613; VC_1456
GeneIDi2613962
KEGGivch:VC1456
PATRICifig|243277.26.peg.1386

Phylogenomic databases

KOiK10929
OMAiHIDSQKK

Enzyme and pathway databases

BioCyciMetaCyc:VC1456-MONOMER
VCHO:VC1456-MONOMER

Miscellaneous databases

EvolutionaryTraceiP01556
PROiPR:P01556

Family and domain databases

InterProiView protein in InterPro
IPR008992 Enterotoxin
IPR001835 Enterotoxin_B
PfamiView protein in Pfam
PF01376 Enterotoxin_b, 1 hit
PRINTSiPR00772 ENTEROTOXINB
ProDomiView protein in ProDom or Entries sharing at least one domain
PD012805 Enterotoxin_B, 1 hit
SUPFAMiSSF50203 SSF50203, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCHTB_VIBCH
AccessioniPrimary (citable) accession number: P01556
Secondary accession number(s): Q9JQ02
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 13, 1987
Last modified: July 18, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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