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Protein

Delta-actitoxin-Avd2a

Gene
N/A
Organism
Anemonia sulcata (Mediterranean snakelocks sea anemone)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Specific arthropod (crab and insect) toxin that inhibits inactivation of voltage-gated sodium channels. It competes well with the site-3 toxin LqhalphaIT (from the scorpion L.quinquestriatus (AC P17728)) on binding to cockroach neuronal membranes (Ki=21.4 nM), and inhibits the inactivation of D.melanogaster channel (DmNav1), but not that of mammalian Navs expressed in Xenopus oocytes. Its activity is synergically enhanced by ligands of receptor site-4 (Bj-xtrIT (AC P56637)). Its ability to inhibit the channel mutant DmNav1[D1701R] only decreases 5-fold, whereas the inhibition activity is completely lost by LqhalphaIT and Av2 when tested on DmNav1[D1701R].3 Publications

Miscellaneous

Is inactive on mammals, when tested by subcutaneous injection into mice at a high dose (up to 5940 nmol/kg of mouse). Does not inhibit rNav1.2/SCN2A, rNav1.4/SCN4A, hNav1.5/SCN5A and rNav1.6/SCN8A.1 Publication

Caution

Opinions are divided on whether Anemonia viridis (Forsskal, 1775) and Anemonia sulcata (Pennant, 1777) are separate species. Authors from PubMed:17492942 and PubMed:19609479 consider they are only one species, it is why their studies (on A.viridis) are integrated in this entry on A.sulcata.Curated

GO - Molecular functioni

Keywordsi

Molecular functionIon channel impairing toxin, Neurotoxin, Toxin, Voltage-gated sodium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-actitoxin-Avd2a1 Publication
Short name:
Delta-AITX-Avd2a1 Publication
Alternative name(s):
ATX III1 Publication
Av31 Publication
Neurotoxin 3
Neurotoxin III2 Publications
OrganismiAnemonia sulcata (Mediterranean snakelocks sea anemone)
Taxonomic identifieri6108 [NCBI]
Taxonomic lineageiEukaryotaMetazoaCnidariaAnthozoaHexacoralliaActiniariaActiniidaeAnemonia

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nematocyst, Secreted

Pathology & Biotechi

Toxic dosei

Dose that causes irreversible contraction paralysis (ED(50)) is 1.15 pmol/100 mg (native toxin) or 2.65 pmol/100 mg (recombinant toxin) when injected inter-segmentally into blowfly larvae (S.falculata).1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1R → A: Low decrease in binding affinity to cockroach neuronal membranes and in toxicity to blowfly larvae. 1 Publication1
Mutagenesisi2S → A: No change in binding affinity to cockroach neuronal membranes and in toxicity to blowfly larvae. 1 Publication1
Mutagenesisi5P → A: Low decrease in binding affinity to cockroach neuronal membranes and in toxicity to blowfly larvae. 1 Publication1
Mutagenesisi7Y → A: Loss of binding affinity to cockroach neuronal membranes and of toxicity to blowfly larvae. 1 Publication1
Mutagenesisi8W → A: Loss of binding affinity to cockroach neuronal membranes and of toxicity to blowfly larvae. 1 Publication1
Mutagenesisi12P → A: Loss of binding affinity to cockroach neuronal membranes and of toxicity to blowfly larvae. 1 Publication1
Mutagenesisi13W → A: Loss of binding affinity to cockroach neuronal membranes and of toxicity to blowfly larvae. 1 Publication1
Mutagenesisi15Q → A: No change in binding affinity to cockroach neuronal membranes and in toxicity to blowfly larvae. 1 Publication1
Mutagenesisi16N → A: No change in binding affinity to cockroach neuronal membranes and in toxicity to blowfly larvae. 1 Publication1
Mutagenesisi18Y → A: Loss of binding affinity to cockroach neuronal membranes and of toxicity to blowfly larvae. 1 Publication1
Mutagenesisi19P → A: Low decrease in binding affinity to cockroach neuronal membranes and in toxicity to blowfly larvae. 1 Publication1
Mutagenesisi20E → A: Low increase in binding affinity to cockroach neuronal membranes and in toxicity to blowfly larvae. 1 Publication1
Mutagenesisi23S → A: Low increase in binding affinity to cockroach neuronal membranes and in toxicity to blowfly larvae. 1 Publication1
Mutagenesisi25P → A: Low increase in binding affinity to cockroach neuronal membranes and in toxicity to blowfly larvae. 1 Publication1
Mutagenesisi26K → A: No change in binding affinity to cockroach neuronal membranes and in toxicity to blowfly larvae. 1 Publication1
Mutagenesisi27V → A: No change in binding affinity to cockroach neuronal membranes and in toxicity to blowfly larvae. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PeptideiPRO_00000448601 – 27Delta-actitoxin-Avd2a1 PublicationAdd BLAST27

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi3 ↔ 172 Publications
Disulfide bondi4 ↔ 112 Publications
Disulfide bondi6 ↔ 222 Publications

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP01535

Structurei

Secondary structure

127
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni7 – 10Combined sources4
Beta strandi12 – 14Combined sources3
Beta strandi22 – 25Combined sources4

3D structure databases

ProteinModelPortaliP01535
SMRiP01535
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP01535

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiView protein in InterPro
IPR016330 Neurotoxin_3_Actiniidae
IPR012509 Neurotoxin_3_Anemonia
IPR036247 Neurotoxin_3_sf
PfamiView protein in Pfam
PF08098 ATX_III, 1 hit
PIRSFiPIRSF001906 Neurotoxin_III_Actiniidae, 1 hit
SUPFAMiSSF57419 SSF57419, 1 hit

Sequencei

Sequence statusi: Complete.

P01535-1 [UniParc]FASTAAdd to basket

« Hide

        10         20 
RSCCPCYWGG CPWGQNCYPE GCSGPKV
Length:27
Mass (Da):2,938
Last modified:July 21, 1986 - v1
Checksum:iAA4E261FFAF34A7A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti22 – 23CS → SC AA sequence (PubMed:21843).Curated2

Sequence databases

PIRiA91446 TZAZ3

Similar proteinsi

Entry informationi

Entry nameiSTX3_ANESU
AccessioniPrimary (citable) accession number: P01535
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 23, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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