UniProtKB - P01326 (INS2_MOUSE)
Protein
Insulin-2
Gene
Ins2
Organism
Mus musculus (Mouse)
Status
Functioni
Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
GO - Molecular functioni
- hormone activity Source: MGI
- identical protein binding Source: MGI
- insulin-like growth factor receptor binding Source: MGI
- insulin receptor binding Source: MGI
- protease binding Source: MGI
- protein-containing complex binding Source: MGI
GO - Biological processi
- activation of MAPK activity Source: MGI
- activation of protein kinase B activity Source: MGI
- acute-phase response Source: MGI
- alpha-beta T cell activation Source: MGI
- canonical Wnt signaling pathway Source: MGI
- ER overload response Source: MGI
- fatty acid homeostasis Source: MGI
- glucose homeostasis Source: MGI
- glucose metabolic process Source: MGI
- glucose transmembrane transport Source: MGI
- G protein-coupled receptor signaling pathway Source: MGI
- insulin receptor signaling pathway Source: MGI
- intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: MGI
- lipid catabolic process Source: MGI
- myoblast fusion Source: MGI
- myotube differentiation Source: MGI
- negative regulation of acute inflammatory response Source: MGI
- negative regulation of fatty acid metabolic process Source: MGI
- negative regulation of feeding behavior Source: MGI
- negative regulation of glycogen catabolic process Source: MGI
- negative regulation of lipid catabolic process Source: MGI
- negative regulation of NAD(P)H oxidase activity Source: MGI
- negative regulation of protein catabolic process Source: MGI
- negative regulation of protein secretion Source: MGI
- negative regulation of proteolysis Source: MGI
- negative regulation of reactive oxygen species biosynthetic process Source: MGI
- negative regulation of respiratory burst involved in inflammatory response Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- neuron projection maintenance Source: MGI
- positive regulation of cell population proliferation Source: MGI
- positive regulation of cellular protein metabolic process Source: MGI
- positive regulation of cytokine secretion Source: MGI
- positive regulation of dendritic spine maintenance Source: MGI
- positive regulation of gene expression Source: MGI
- positive regulation of glucose import Source: MGI
- positive regulation of glycogen biosynthetic process Source: MGI
- positive regulation of glycolytic process Source: MGI
- positive regulation of insulin receptor signaling pathway Source: MGI
- positive regulation of MAPK cascade Source: MGI
- positive regulation of mitotic nuclear division Source: MGI
- positive regulation of NF-kappaB transcription factor activity Source: MGI
- positive regulation of nitric oxide mediated signal transduction Source: MGI
- positive regulation of peptidyl-tyrosine phosphorylation Source: MGI
- positive regulation of phosphatidylinositol 3-kinase activity Source: MGI
- positive regulation of phosphatidylinositol 3-kinase signaling Source: MGI
- positive regulation of protein kinase B signaling Source: MGI
- positive regulation of protein localization to nucleus Source: MGI
- positive regulation of respiratory burst Source: MGI
- regulation of cellular amino acid metabolic process Source: MGI
- regulation of gene expression Source: MGI
- regulation of phosphorylation Source: MGI
- regulation of protein binding Source: MGI
- regulation of protein localization Source: MGI
- regulation of protein localization to plasma membrane Source: MGI
- regulation of protein secretion Source: MGI
- regulation of transmembrane transporter activity Source: MGI
- response to organic substance Source: GO_Central
- wound healing Source: MGI
Keywordsi
Molecular function | Hormone |
Biological process | Carbohydrate metabolism, Glucose metabolism |
Enzyme and pathway databases
Reactomei | R-MMU-264876, Insulin processing R-MMU-422085, Synthesis, secretion, and deacylation of Ghrelin R-MMU-6807878, COPI-mediated anterograde transport R-MMU-6811558, PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling R-MMU-74713, IRS activation R-MMU-74749, Signal attenuation R-MMU-74751, Insulin receptor signalling cascade R-MMU-74752, Signaling by Insulin receptor R-MMU-77387, Insulin receptor recycling |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Ins2 Synonyms:Ins-2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:96573, Ins2 |
Subcellular locationi
Extracellular region or secreted
Cytosol
- cytosol Source: MGI
Extracellular region or secreted
- collagen-containing extracellular matrix Source: BHF-UCL
- extracellular region Source: Reactome
- extracellular space Source: MGI
Nucleus
- nucleus Source: MGI
Other locations
- cytoplasm Source: MGI
- secretory granule Source: MGI
- secretory granule lumen Source: Reactome
- small nucleolar ribonucleoprotein complex Source: MGI
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 24 | 1 PublicationAdd BLAST | 24 | |
PeptideiPRO_0000015845 | 25 – 54 | Insulin-2 B chain1 PublicationAdd BLAST | 30 | |
PropeptideiPRO_0000015846 | 57 – 87 | C peptideAdd BLAST | 31 | |
PeptideiPRO_0000015847 | 90 – 110 | Insulin-2 A chain1 PublicationAdd BLAST | 21 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 31 ↔ 96 | Interchain (between B and A chains)By similarity | ||
Disulfide bondi | 43 ↔ 109 | Interchain (between B and A chains)By similarity | ||
Disulfide bondi | 95 ↔ 100 | By similarity |
Keywords - PTMi
Cleavage on pair of basic residues, Disulfide bondProteomic databases
PaxDbi | P01326 |
PRIDEi | P01326 |
PTM databases
iPTMneti | P01326 |
PhosphoSitePlusi | P01326 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000000215, Expressed in dorsal pancreas and 89 other tissues |
Genevisiblei | P01326, MM |
Interactioni
Subunit structurei
Heterodimer of a B chain and an A chain linked by two disulfide bonds.
By similarityGO - Molecular functioni
- hormone activity Source: MGI
- identical protein binding Source: MGI
- insulin-like growth factor receptor binding Source: MGI
- insulin receptor binding Source: MGI
- protease binding Source: MGI
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000101553 |
Miscellaneous databases
RNActi | P01326, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the insulin family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG502S5P5, Eukaryota |
GeneTreei | ENSGT00390000015440 |
InParanoidi | P01326 |
OMAi | CCTGICS |
OrthoDBi | 1644517at2759 |
PhylomeDBi | P01326 |
TreeFami | TF332820 |
Family and domain databases
CDDi | cd04367, IlGF_insulin_like, 1 hit |
InterProi | View protein in InterPro IPR004825, Insulin IPR016179, Insulin-like IPR036438, Insulin-like_sf IPR022353, Insulin_CS IPR022352, Insulin_family |
PANTHERi | PTHR11454, PTHR11454, 1 hit |
Pfami | View protein in Pfam PF00049, Insulin, 1 hit |
PRINTSi | PR00277, INSULIN PR00276, INSULINFAMLY |
SMARTi | View protein in SMART SM00078, IlGF, 1 hit |
SUPFAMi | SSF56994, SSF56994, 1 hit |
PROSITEi | View protein in PROSITE PS00262, INSULIN, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
P01326-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALWMRFLPL LALLFLWESH PTQAFVKQHL CGSHLVEALY LVCGERGFFY
60 70 80 90 100
TPMSRREVED PQVAQLELGG GPGAGDLQTL ALEVAQQKRG IVDQCCTSIC
110
SLYQLENYCN
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE0CXX7 | E0CXX7_MOUSE | Insulin | Ins2 | 98 | Annotation score: | ||
D3Z596 | D3Z596_MOUSE | Insulin-2 | Ins2 | 79 | Annotation score: | ||
D3Z595 | D3Z595_MOUSE | Insulin-2 | Ins2 | 106 | Annotation score: | ||
D3YWU5 | D3YWU5_MOUSE | Insulin-2 | Ins2 | 70 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04724 Genomic DNA Translation: CAA28433.1 |
CCDSi | CCDS22034.1 |
PIRi | A26342, INMS2 |
RefSeqi | NP_001172012.1, NM_001185083.2 NP_001172013.1, NM_001185084.2 NP_032413.1, NM_008387.5 |
Genome annotation databases
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04724 Genomic DNA Translation: CAA28433.1 |
CCDSi | CCDS22034.1 |
PIRi | A26342, INMS2 |
RefSeqi | NP_001172012.1, NM_001185083.2 NP_001172013.1, NM_001185084.2 NP_032413.1, NM_008387.5 |
3D structure databases
SMRi | P01326 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000101553 |
PTM databases
iPTMneti | P01326 |
PhosphoSitePlusi | P01326 |
Proteomic databases
PaxDbi | P01326 |
PRIDEi | P01326 |
Protocols and materials databases
ABCDi | P01326, 1 sequenced antibody |
Genome annotation databases
Organism-specific databases
CTDi | 16334 |
MGIi | MGI:96573, Ins2 |
Phylogenomic databases
eggNOGi | ENOG502S5P5, Eukaryota |
GeneTreei | ENSGT00390000015440 |
InParanoidi | P01326 |
OMAi | CCTGICS |
OrthoDBi | 1644517at2759 |
PhylomeDBi | P01326 |
TreeFami | TF332820 |
Enzyme and pathway databases
Reactomei | R-MMU-264876, Insulin processing R-MMU-422085, Synthesis, secretion, and deacylation of Ghrelin R-MMU-6807878, COPI-mediated anterograde transport R-MMU-6811558, PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling R-MMU-74713, IRS activation R-MMU-74749, Signal attenuation R-MMU-74751, Insulin receptor signalling cascade R-MMU-74752, Signaling by Insulin receptor R-MMU-77387, Insulin receptor recycling |
Miscellaneous databases
BioGRID-ORCSi | 16334, 1 hit in 17 CRISPR screens |
ChiTaRSi | Ins2, mouse |
PROi | PR:P01326 |
RNActi | P01326, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000000215, Expressed in dorsal pancreas and 89 other tissues |
Genevisiblei | P01326, MM |
Family and domain databases
CDDi | cd04367, IlGF_insulin_like, 1 hit |
InterProi | View protein in InterPro IPR004825, Insulin IPR016179, Insulin-like IPR036438, Insulin-like_sf IPR022353, Insulin_CS IPR022352, Insulin_family |
PANTHERi | PTHR11454, PTHR11454, 1 hit |
Pfami | View protein in Pfam PF00049, Insulin, 1 hit |
PRINTSi | PR00277, INSULIN PR00276, INSULINFAMLY |
SMARTi | View protein in SMART SM00078, IlGF, 1 hit |
SUPFAMi | SSF56994, SSF56994, 1 hit |
PROSITEi | View protein in PROSITE PS00262, INSULIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | INS2_MOUSE | |
Accessioni | P01326Primary (citable) accession number: P01326 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | August 13, 1987 | |
Last modified: | December 2, 2020 | |
This is version 188 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families