Ins2

Functionⁱ

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

GO - Molecular functionⁱ

hormone activity Source: MGI
identical protein binding Source: MGI
insulin-like growth factor receptor binding Source: MGI
insulin receptor binding Source: MGI
protease binding Source: MGI
protein-containing complex binding Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

activation of MAPK activity
Source: MGI
Inferred from direct assayⁱ
- 12059784
activation of protein kinase B activity Source: MGI
acute-phase response Source: MGI
alpha-beta T cell activation Source: MGI
canonical Wnt signaling pathway
Source: MGI
Inferred from genetic interactionⁱ
- 15282335
ER overload response
Source: MGI
Inferred from mutant phenotypeⁱ
- 11854325
fatty acid homeostasis Source: MGI
glucose homeostasis
Source: MGI
Inferred from mutant phenotypeⁱ
- 23274896
glucose metabolic process
Source: MGI
Inferred from mutant phenotypeⁱ
- 11854325
glucose transmembrane transport
Source: MGI
Traceable author statementⁱ
- 12297296
G protein-coupled receptor signaling pathway Source: MGI
insulin receptor signaling pathway
Source: MGI
Inferred from direct assayⁱ
- 12805218
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
Source: MGI
Inferred from mutant phenotypeⁱ
- 11854325
lipid catabolic process
Source: MGI
Inferred from direct assayⁱ
- 12805218
MAPK cascade Source: MGI
myoblast fusion
Source: MGI
Inferred from genetic interactionⁱ
- 15282335
myotube differentiation
Source: MGI
Inferred from genetic interactionⁱ
- 15282335
negative regulation of acute inflammatory response Source: MGI
negative regulation of fatty acid metabolic process Source: MGI
negative regulation of feeding behavior Source: MGI
negative regulation of glycogen catabolic process Source: MGI
negative regulation of lipid catabolic process Source: MGI
negative regulation of NAD(P)H oxidase activity Source: MGI
negative regulation of protein catabolic process Source: MGI
negative regulation of protein oligomerization Source: MGI
negative regulation of protein secretion Source: MGI
negative regulation of proteolysis Source: MGI
negative regulation of reactive oxygen species biosynthetic process Source: MGI
negative regulation of respiratory burst involved in inflammatory response Source: MGI
negative regulation of transcription by RNA polymerase II
Source: MGI
Inferred from direct assayⁱ
- 9689059
neuron projection maintenance Source: MGI
positive regulation of cell proliferation Source: MGI
positive regulation of cellular protein metabolic process Source: MGI
positive regulation of cytokine secretion Source: MGI
positive regulation of dendritic spine maintenance Source: MGI
positive regulation of gene expression Source: MGI
positive regulation of glucose import Source: MGI
positive regulation of glucose import in response to insulin stimulus Source: MGI
positive regulation of glycogen biosynthetic process Source: MGI
positive regulation of glycolytic process Source: MGI
positive regulation of insulin receptor signaling pathway Source: MGI
positive regulation of MAPK cascade Source: MGI
positive regulation of mitotic nuclear division Source: MGI
positive regulation of NF-kappaB transcription factor activity Source: MGI
positive regulation of nitric oxide mediated signal transduction Source: MGI
positive regulation of peptidyl-tyrosine phosphorylation
Source: MGI
Inferred from direct assayⁱ
- 23775122
positive regulation of phosphatidylinositol 3-kinase activity
Source: MGI
Inferred from direct assayⁱ
- 11375348
positive regulation of phosphatidylinositol 3-kinase signaling Source: MGI
positive regulation of protein kinase B signaling Source: MGI
positive regulation of protein localization to nucleus Source: MGI
positive regulation of respiratory burst Source: MGI
regulation of cellular amino acid metabolic process Source: MGI
regulation of gene expression
Source: MGI
Inferred from direct assayⁱ
- 19293336
regulation of phosphorylation
Source: MGI
Inferred from direct assayⁱ
- 12059784
regulation of protein binding
Source: MGI
Inferred from direct assayⁱ
- 9507006
regulation of protein localization Source: MGI
regulation of protein localization to plasma membrane Source: MGI
regulation of protein secretion Source: MGI
regulation of transmembrane transporter activity Source: MGI
response to organic substance
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
wound healing Source: MGI

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Hormone
Biological process	Carbohydrate metabolism, Glucose metabolism

Enzyme and pathway databases

Reactomeⁱ	R-MMU-264876 Insulin processing R-MMU-422085 Synthesis, secretion, and deacylation of Ghrelin R-MMU-6807878 COPI-mediated anterograde transport R-MMU-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling R-MMU-74713 IRS activation R-MMU-74749 Signal attenuation R-MMU-74751 Insulin receptor signalling cascade R-MMU-74752 Signaling by Insulin receptor R-MMU-77387 Insulin receptor recycling

Reactomeⁱ

R-MMU-264876 Insulin processing
R-MMU-422085 Synthesis, secretion, and deacylation of Ghrelin
R-MMU-6807878 COPI-mediated anterograde transport
R-MMU-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling
R-MMU-74713 IRS activation
R-MMU-74749 Signal attenuation
R-MMU-74751 Insulin receptor signalling cascade
R-MMU-74752 Signaling by Insulin receptor
R-MMU-77387 Insulin receptor recycling

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Insulin-2 Cleaved into the following 2 chains: Insulin-2 B chain Insulin-2 A chain
Gene namesⁱ	Name:Ins2 Synonyms:Ins-2
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 7

Organism-specific databases

MGIⁱ	MGI:96573 Ins2

Keywords - Cellular componentⁱ

Secreted

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Length
Signal peptideⁱ	1 – 24	1 PublicationAdd BLAST	24
PeptideⁱPRO_0000015845	25 – 54	Insulin-2 B chain1 PublicationAdd BLAST	30
PropeptideⁱPRO_0000015846	57 – 87	C peptideAdd BLAST	31
PeptideⁱPRO_0000015847	90 – 110	Insulin-2 A chain1 PublicationAdd BLAST	21

Amino acid modifications

Feature key	Position(s)	DescriptionActions
Disulfide bondⁱ	31 ↔ 96	Interchain (between B and A chains)By similarity
Disulfide bondⁱ	43 ↔ 109	Interchain (between B and A chains)By similarity
Disulfide bondⁱ	95 ↔ 100	By similarity

Keywords - PTMⁱ

Cleavage on pair of basic residues, Disulfide bond

Proteomic databases

PaxDbⁱ	P01326
PRIDEⁱ	P01326

PTM databases

PhosphoSitePlusⁱ	P01326

PhosphoSitePlusⁱ

P01326

Miscellaneous databases

PMAP-CutDBⁱ	P01326

PMAP-CutDBⁱ

P01326

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000000215 Expressed in 67 organ(s), highest expression level in dorsal pancreas
CleanExⁱ	MM_INS2
ExpressionAtlasⁱ	P01326 baseline and differential
Genevisibleⁱ	P01326 MM

Interactionⁱ

Subunit structureⁱ

Heterodimer of a B chain and an A chain linked by two disulfide bonds.By similarity

GO - Molecular functionⁱ

hormone activity Source: MGI
identical protein binding Source: MGI
insulin-like growth factor receptor binding Source: MGI
insulin receptor binding Source: MGI
protease binding Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

STRINGⁱ	10090.ENSMUSP00000000220

STRINGⁱ

10090.ENSMUSP00000000220

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P01326
SMRⁱ	P01326
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the insulin family.Curated

Keywords - Domainⁱ

Signal

Phylogenomic databases

eggNOGⁱ	ENOG410J0XC Eukaryota ENOG4111VJB LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000015440
HOGENOMⁱ	HOG000261669
HOVERGENⁱ	HBG006137
InParanoidⁱ	P01326
KEGG Orthology (KO) More...KOⁱ	K04526
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0H56
PhylomeDBⁱ	P01326
TreeFamⁱ	TF332820

Family and domain databases

InterProⁱ	View protein in InterPro IPR004825 Insulin IPR016179 Insulin-like IPR036438 Insulin-like_sf IPR022353 Insulin_CS IPR022352 Insulin_family
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00049 Insulin, 1 hit
PRINTSⁱ	PR00277 INSULIN PR00276 INSULINFAMLY
SMARTⁱ	View protein in SMART SM00078 IlGF, 1 hit
SUPFAMⁱ	SSF56994 SSF56994, 1 hit
PROSITEⁱ	View protein in PROSITE PS00262 INSULIN, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show all Align All

P01326-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MALWMRFLPL LALLFLWESH PTQAFVKQHL CGSHLVEALY LVCGERGFFY 
        60         70         80         90        100
TPMSRREVED PQVAQLELGG GPGAGDLQTL ALEVAQQKRG IVDQCCTSIC 
       110
SLYQLENYCN

Length:110

Mass (Da):12,364

Last modified:August 13, 1987 - v1

Checksum:ⁱ3554C8803D24FDAD

GO

Computationally mapped potential isoform sequencesⁱ

There are 4 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

E0CXX7	E0CXX7_MOUSE	Insulin Insulin	Ins2	98	Annotation score:
D3Z596	D3Z596_MOUSE	Insulin-2 Insulin-2	Ins2	79	Annotation score:
D3Z595	D3Z595_MOUSE	Insulin-2 Insulin-2	Ins2	106	Annotation score:
D3YWU5	D3YWU5_MOUSE	Insulin-2 Insulin-2	Ins2	70	Annotation score:

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X04724 Genomic DNA Translation: CAA28433.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS22034.1
PIRⁱ	A26342 INMS2
NCBI Reference Sequences More...RefSeqⁱ	NP_001172012.1, NM_001185083.2 NP_001172013.1, NM_001185084.2 NP_032413.1, NM_008387.5
UniGeneⁱ	Mm.4946

Genome annotation databases

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P01326	Insulin		MOUSE		110	UniRef100_P01326
Insulin		MUSSP		110
Insulin (Fragment)		MOUSE		109

Protein	Similar proteins		Species	Score	Length	Source
P01326	Insulin		CRILO		110	UniRef90_P01326
Insulin-2		RAT		110
Insulin		MOUSE		110
Insulin		MUSSP		110
Insulin		Coxing's white-bellied rat		110
+12

Protein	Similar proteins		Species	Score	Length	Source
P01326	Insulin	)	HUMAN		110	UniRef50_P01308
Insulin		GORGO		110
Insulin		PONPY		110
Insulin		PSAOB		110
Insulin		CHICK		107
+88

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X04724 Genomic DNA Translation: CAA28433.1
CCDSⁱ	CCDS22034.1
PIRⁱ	A26342 INMS2
RefSeqⁱ	NP_001172012.1, NM_001185083.2 NP_001172013.1, NM_001185084.2 NP_032413.1, NM_008387.5
UniGeneⁱ	Mm.4946

3D structure databases

ProteinModelPortalⁱ	P01326
SMRⁱ	P01326
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	10090.ENSMUSP00000000220

STRINGⁱ

10090.ENSMUSP00000000220

PTM databases

PhosphoSitePlusⁱ	P01326

PhosphoSitePlusⁱ

P01326

Proteomic databases

PaxDbⁱ	P01326
PRIDEⁱ	P01326

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000000220; ENSMUSP00000000220; ENSMUSG00000000215 ENSMUST00000105932; ENSMUSP00000101552; ENSMUSG00000000215 ENSMUST00000105933; ENSMUSP00000101553; ENSMUSG00000000215 ENSMUST00000105934; ENSMUSP00000101554; ENSMUSG00000000215 ENSMUST00000210288; ENSMUSP00000147425; ENSMUSG00000000215
GeneIDⁱ	16334
KEGGⁱ	mmu:16334
UCSCⁱ	uc009kof.2 mouse

Organism-specific databases

CTDⁱ	16334
MGIⁱ	MGI:96573 Ins2

Phylogenomic databases

eggNOGⁱ	ENOG410J0XC Eukaryota ENOG4111VJB LUCA
GeneTreeⁱ	ENSGT00390000015440
HOGENOMⁱ	HOG000261669
HOVERGENⁱ	HBG006137
InParanoidⁱ	P01326
KOⁱ	K04526
OrthoDBⁱ	EOG091G0H56
PhylomeDBⁱ	P01326
TreeFamⁱ	TF332820

Enzyme and pathway databases

Reactomeⁱ	R-MMU-264876 Insulin processing R-MMU-422085 Synthesis, secretion, and deacylation of Ghrelin R-MMU-6807878 COPI-mediated anterograde transport R-MMU-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling R-MMU-74713 IRS activation R-MMU-74749 Signal attenuation R-MMU-74751 Insulin receptor signalling cascade R-MMU-74752 Signaling by Insulin receptor R-MMU-77387 Insulin receptor recycling

Reactomeⁱ

R-MMU-264876 Insulin processing
R-MMU-422085 Synthesis, secretion, and deacylation of Ghrelin
R-MMU-6807878 COPI-mediated anterograde transport
R-MMU-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling
R-MMU-74713 IRS activation
R-MMU-74749 Signal attenuation
R-MMU-74751 Insulin receptor signalling cascade
R-MMU-74752 Signaling by Insulin receptor
R-MMU-77387 Insulin receptor recycling

Miscellaneous databases

ChiTaRSⁱ	Ins2 mouse
PMAP-CutDBⁱ	P01326
Protein Ontology More...PROⁱ	PR:P01326
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000000215 Expressed in 67 organ(s), highest expression level in dorsal pancreas
CleanExⁱ	MM_INS2
ExpressionAtlasⁱ	P01326 baseline and differential
Genevisibleⁱ	P01326 MM

Family and domain databases

InterProⁱ	View protein in InterPro IPR004825 Insulin IPR016179 Insulin-like IPR036438 Insulin-like_sf IPR022353 Insulin_CS IPR022352 Insulin_family
Pfamⁱ	View protein in Pfam PF00049 Insulin, 1 hit
PRINTSⁱ	PR00277 INSULIN PR00276 INSULINFAMLY
SMARTⁱ	View protein in SMART SM00078 IlGF, 1 hit
SUPFAMⁱ	SSF56994 SSF56994, 1 hit
PROSITEⁱ	View protein in PROSITE PS00262 INSULIN, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	INS2_MOUSE
Accessionⁱ	P01326Primary (citable) accession number: P01326
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
	Last sequence update:	August 13, 1987
	Last modified:	November 7, 2018
	This is version 174 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Direct protein sequencing, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P01326 (INS2_MOUSE)

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Insulin-2

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted

Cytosol

Extracellular region or secreted

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

Miscellaneous databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ