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UniProtKB - P01317 (INS_BOVIN)
Protein
Insulin
Gene
INS
Organism
Bos taurus (Bovine)
Status
Functioni
Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
GO - Molecular functioni
- hormone activity Source: UniProtKB-KW
- identical protein binding Source: IntAct
- insulin receptor binding Source: AgBase
GO - Biological processi
- estradiol secretion Source: AgBase
- feeding behavior Source: AgBase
- glucose import in response to insulin stimulus Source: AgBase
- glucose metabolic process Source: UniProtKB-KW
- negative regulation of apoptotic process Source: AgBase
- negative regulation of appetite Source: AgBase
- negative regulation of lactation Source: AgBase
- negative regulation of lipid catabolic process Source: AgBase
- positive regulation of blood circulation Source: AgBase
- positive regulation of cell maturation Source: AgBase
- positive regulation of gene expression Source: AgBase
- positive regulation of insulin secretion Source: AgBase
- positive regulation of lactation Source: AgBase
- positive regulation of mammary gland epithelial cell proliferation Source: AgBase
- positive regulation of peptide hormone secretion Source: AgBase
- positive regulation of phosphatidylinositol 3-kinase signaling Source: AgBase
- positive regulation of protein secretion Source: AgBase
- positive regulation of Rho protein signal transduction Source: AgBase
- protein secretion Source: AgBase
- response to butyrate Source: AgBase
- response to food Source: AgBase
- response to glucose Source: AgBase
- response to growth hormone Source: AgBase
- response to heat Source: AgBase
- response to L-arginine Source: AgBase
- response to nutrient levels Source: AgBase
Keywordsi
Molecular function | Hormone |
Biological process | Carbohydrate metabolism, Glucose metabolism |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:INS |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular space Source: AgBase
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 24 | 3 PublicationsAdd BLAST | 24 | |
PeptideiPRO_0000015764 | 25 – 54 | Insulin B chain1 PublicationAdd BLAST | 30 | |
PropeptideiPRO_0000015765 | 57 – 82 | C peptideAdd BLAST | 26 | |
PeptideiPRO_0000015766 | 85 – 105 | Insulin A chain1 PublicationAdd BLAST | 21 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 31 ↔ 91 | Interchain (between B and A chains)1 Publication | ||
Disulfide bondi | 43 ↔ 104 | Interchain (between B and A chains)1 Publication | ||
Disulfide bondi | 90 ↔ 95 | 2 Publications |
Keywords - PTMi
Cleavage on pair of basic residues, Disulfide bondProteomic databases
PaxDbi | P01317 |
PTM databases
CarbonylDBi | P01317 |
Interactioni
Subunit structurei
Heterodimer of a B chain and an A chain linked by two disulfide bonds.
1 PublicationBinary interactionsi
P01317
GO - Molecular functioni
- hormone activity Source: UniProtKB-KW
- identical protein binding Source: IntAct
- insulin receptor binding Source: AgBase
Protein-protein interaction databases
DIPi | DIP-52901N |
IntActi | P01317, 5 interactors |
MINTi | P01317 |
STRINGi | 9913.ENSBTAP00000017289 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P01317 |
BMRBi | P01317 |
SMRi | P01317 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P01317 |
Family & Domainsi
Sequence similaritiesi
Belongs to the insulin family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG502S5P5, Eukaryota |
InParanoidi | P01317 |
OrthoDBi | 1644517at2759 |
Family and domain databases
CDDi | cd04367, IlGF_insulin_like, 1 hit |
InterProi | View protein in InterPro IPR004825, Insulin IPR016179, Insulin-like IPR036438, Insulin-like_sf IPR022353, Insulin_CS IPR022352, Insulin_family |
PANTHERi | PTHR11454, PTHR11454, 1 hit |
Pfami | View protein in Pfam PF00049, Insulin, 1 hit |
PRINTSi | PR00277, INSULIN PR00276, INSULINFAMLY |
SMARTi | View protein in SMART SM00078, IlGF, 1 hit |
SUPFAMi | SSF56994, SSF56994, 1 hit |
PROSITEi | View protein in PROSITE PS00262, INSULIN, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P01317-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALWTRLRPL LALLALWPPP PARAFVNQHL CGSHLVEALY LVCGERGFFY
60 70 80 90 100
TPKARREVEG PQVGALELAG GPGAGGLEGP PQKRGIVEQC CASVCSLYQL
ENYCN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M54979 mRNA Translation: AAA30722.1 |
PIRi | A40909, IPBO |
Similar proteinsi
Cross-referencesi
Web resourcesi
Protein Spotlight Protein of the 20th century - Issue 9 of April 2001 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M54979 mRNA Translation: AAA30722.1 |
PIRi | A40909, IPBO |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1APH | X-ray | 2.00 | A | 85-105 | [»] | |
B | 25-54 | [»] | ||||
1BPH | X-ray | 2.00 | A | 85-105 | [»] | |
B | 25-54 | [»] | ||||
1CPH | X-ray | 1.90 | A | 85-105 | [»] | |
B | 25-54 | [»] | ||||
1DPH | X-ray | 1.90 | A | 85-105 | [»] | |
B | 25-54 | [»] | ||||
1HO0 | NMR | - | A | 25-54 | [»] | |
1PID | X-ray | 1.30 | A/C | 85-105 | [»] | |
B/D | 25-49 | [»] | ||||
2A3G | X-ray | 2.25 | A/C | 85-105 | [»] | |
B/D | 25-54 | [»] | ||||
2BN1 | X-ray | 1.40 | A | 85-105 | [»] | |
B | 25-54 | [»] | ||||
2BN3 | X-ray | 1.40 | A | 85-105 | [»] | |
B | 25-54 | [»] | ||||
2INS | X-ray | 2.50 | A/C | 85-105 | [»] | |
B/D | 26-54 | [»] | ||||
2ZP6 | X-ray | 2.56 | A/C | 85-105 | [»] | |
B/D | 25-54 | [»] | ||||
4BS3 | X-ray | 2.30 | A | 85-105 | [»] | |
B | 25-54 | [»] | ||||
4E7T | X-ray | 1.40 | A/C | 85-105 | [»] | |
B/D | 25-54 | [»] | ||||
4E7U | X-ray | 1.30 | A/C | 85-105 | [»] | |
B/D | 25-54 | [»] | ||||
4E7V | X-ray | 1.80 | 1/3/5/A/C/E/G/I/K/M/O/Q/S/U/W/Y | 85-105 | [»] | |
2/4/6/B/D/F/H/J/L/N/P/R/T/V/X/Z | 25-54 | [»] | ||||
4I5Y | X-ray | 1.80 | A | 85-105 | [»] | |
B | 25-54 | [»] | ||||
4I5Z | X-ray | 1.80 | A | 85-105 | [»] | |
B | 25-54 | [»] | ||||
4IDW | X-ray | 2.70 | A/C | 85-105 | [»] | |
B/D | 25-54 | [»] | ||||
4IHN | X-ray | 1.16 | A | 85-105 | [»] | |
B | 25-54 | [»] | ||||
4M4F | X-ray | 1.90 | A/C | 85-105 | [»] | |
B/D | 25-54 | [»] | ||||
4M4H | X-ray | 1.90 | A/C | 85-105 | [»] | |
B/D | 25-54 | [»] | ||||
4M4I | X-ray | 1.90 | A/C | 85-105 | [»] | |
B/D | 25-54 | [»] | ||||
4M4J | X-ray | 1.90 | A/C | 85-105 | [»] | |
B/D | 25-54 | [»] | ||||
4M4L | X-ray | 1.45 | A/C | 85-105 | [»] | |
B/D | 25-54 | [»] | ||||
4M4M | X-ray | 1.50 | A/C | 85-105 | [»] | |
B/D | 25-54 | [»] | ||||
5AZZ | X-ray | 1.45 | A | 85-105 | [»] | |
B | 25-54 | [»] | ||||
5KQV | X-ray | 4.40 | A/I | 85-105 | [»] | |
B/J | 25-54 | [»] | ||||
5MIZ | NMR | - | A | 85-105 | [»] | |
B | 25-54 | [»] | ||||
6KH8 | NMR | - | A | 85-105 | [»] | |
B | 25-54 | [»] | ||||
6KH9 | NMR | - | A | 85-105 | [»] | |
B | 25-54 | [»] | ||||
6KHA | NMR | - | A | 85-105 | [»] | |
B | 25-54 | [»] | ||||
6OR0 | X-ray | 1.55 | A | 85-105 | [»] | |
B | 25-53 | [»] | ||||
6Q8Q | X-ray | 2.00 | A | 85-105 | [»] | |
B | 25-54 | [»] | ||||
6QQ7 | X-ray | 1.65 | A | 85-105 | [»] | |
B | 25-54 | [»] | ||||
6QQG | X-ray | 2.15 | A | 85-105 | [»] | |
B | 25-54 | [»] | ||||
6QRH | X-ray | 2.15 | A | 85-105 | [»] | |
B | 25-54 | [»] | ||||
6QRK | X-ray | 2.10 | A | 85-105 | [»] | |
B | 25-54 | [»] | ||||
6ZHB | electron microscopy | 3.25 | A/C | 85-105 | [»] | |
B/D | 25-54 | [»] | ||||
6ZI8 | X-ray | 2.30 | A/B/C/D | 1-105 | [»] | |
7ELJ | NMR | - | A | 85-105 | [»] | |
B | 25-54 | [»] | ||||
AlphaFoldDBi | P01317 | |||||
BMRBi | P01317 | |||||
SMRi | P01317 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-52901N |
IntActi | P01317, 5 interactors |
MINTi | P01317 |
STRINGi | 9913.ENSBTAP00000017289 |
Protein family/group databases
Allergomei | 2118, Bos d Insulin |
PTM databases
CarbonylDBi | P01317 |
Proteomic databases
PaxDbi | P01317 |
Protocols and materials databases
ABCDi | P01317, 1 sequenced antibody |
Phylogenomic databases
eggNOGi | ENOG502S5P5, Eukaryota |
InParanoidi | P01317 |
OrthoDBi | 1644517at2759 |
Miscellaneous databases
EvolutionaryTracei | P01317 |
Family and domain databases
CDDi | cd04367, IlGF_insulin_like, 1 hit |
InterProi | View protein in InterPro IPR004825, Insulin IPR016179, Insulin-like IPR036438, Insulin-like_sf IPR022353, Insulin_CS IPR022352, Insulin_family |
PANTHERi | PTHR11454, PTHR11454, 1 hit |
Pfami | View protein in Pfam PF00049, Insulin, 1 hit |
PRINTSi | PR00277, INSULIN PR00276, INSULINFAMLY |
SMARTi | View protein in SMART SM00078, IlGF, 1 hit |
SUPFAMi | SSF56994, SSF56994, 1 hit |
PROSITEi | View protein in PROSITE PS00262, INSULIN, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | INS_BOVIN | |
Accessioni | P01317Primary (citable) accession number: P01317 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | August 1, 1992 | |
Last modified: | May 25, 2022 | |
This is version 186 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Protein Spotlight
Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries