UniProtKB - P01210 (PENK_HUMAN)
Proenkephalin-A
PENK
Functioni
Competes with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress.
Competes with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress.
Increases glutamate release in the striatum and decreases GABA concentration in the striatum.
By similarityIncreases glutamate release in the striatum.
By similarityGO - Molecular functioni
- neuropeptide hormone activity Source: ProtInc
- opioid peptide activity Source: UniProtKB-KW
- opioid receptor binding Source: GO_Central
GO - Biological processi
- aggressive behavior Source: Ensembl
- aging Source: Ensembl
- behavioral fear response Source: Ensembl
- cellular response to cAMP Source: Ensembl
- cellular response to oxidative stress Source: Ensembl
- cellular response to transforming growth factor beta stimulus Source: Ensembl
- cellular response to virus Source: Ensembl
- cellular response to vitamin D Source: Ensembl
- chemical synaptic transmission Source: GO_Central
- general adaptation syndrome, behavioral process Source: Ensembl
- glial cell proliferation Source: Ensembl
- locomotory exploration behavior Source: Ensembl
- neuropeptide signaling pathway Source: GO_Central
- osteoblast differentiation Source: Ensembl
- positive regulation of behavioral fear response Source: Ensembl
- response to calcium ion Source: Ensembl
- response to epinephrine Source: Ensembl
- response to estradiol Source: Ensembl
- response to ethanol Source: Ensembl
- response to hypoxia Source: Ensembl
- response to lipopolysaccharide Source: Ensembl
- response to morphine Source: Ensembl
- response to nicotine Source: Ensembl
- response to radiation Source: Ensembl
- response to toxic substance Source: Ensembl
- sensory perception Source: GO_Central
- sensory perception of pain Source: Ensembl
- signal transduction Source: ProtInc
- startle response Source: Ensembl
- synaptic signaling via neuropeptide Source: Ensembl
Keywordsi
Molecular function | Endorphin, Neuropeptide, Opioid peptide |
Enzyme and pathway databases
PathwayCommonsi | P01210 |
Reactomei | R-HSA-375276, Peptide ligand-binding receptors R-HSA-381426, Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) R-HSA-418594, G alpha (i) signalling events R-HSA-8957275, Post-translational protein phosphorylation |
SignaLinki | P01210 |
SIGNORi | P01210 |
Protein family/group databases
TCDBi | 1.C.89.1.2, the dynorphin channel-forming neuropeptide (dynorphin) family |
Names & Taxonomyi
Protein namesi | Recommended name: Proenkephalin-ACuratedCleaved into the following 8 chains: Alternative name(s): Opioid growth factor Short name: OGF |
Gene namesi | Name:PENKImported |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:8831, PENK |
MIMi | 131330, gene |
neXtProti | NX_P01210 |
VEuPathDBi | HostDB:ENSG00000181195 |
Subcellular locationi
Extracellular region or secreted
- Secreted By similarity
Other locations
- chromaffin granule lumen By similarity
Endoplasmic reticulum
- endoplasmic reticulum lumen Source: Reactome
Extracellular region or secreted
- extracellular region Source: Reactome
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- axon terminus Source: GO_Central
- cell body fiber Source: Ensembl
- chromaffin granule lumen Source: UniProtKB-SubCell
- dendrite Source: GO_Central
- neuronal cell body Source: GO_Central
- neuronal dense core vesicle lumen Source: Ensembl
- perikaryon Source: Ensembl
- symmetric synapse Source: Ensembl
- synaptic vesicle lumen Source: Ensembl
Keywords - Cellular componenti
Cytoplasmic vesicle, SecretedPathology & Biotechi
Organism-specific databases
DisGeNETi | 5179 |
OpenTargetsi | ENSG00000181195 |
PharmGKBi | PA33176 |
Miscellaneous databases
Pharosi | P01210, Tbio |
Genetic variation databases
BioMutai | PENK |
DMDMi | 129770 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 24 | Sequence analysisAdd BLAST | 24 | |
PeptideiPRO_0000008242 | 25 – 97 | SynenkephalinAdd BLAST | 73 | |
PeptideiPRO_0000008243 | 100 – 104 | Met-enkephalin | 5 | |
PeptideiPRO_0000008244 | 107 – 111 | Met-enkephalin | 5 | |
PeptideiPRO_0000377691 | 114 – 133 | PENK(114-133)By similarityAdd BLAST | 20 | |
PeptideiPRO_0000008246 | 136 – 140 | Met-enkephalin | 5 | |
PeptideiPRO_0000377692 | 143 – 183 | PENK(143-183)By similarityAdd BLAST | 41 | |
PeptideiPRO_0000008248 | 186 – 193 | Met-enkephalin-Arg-Gly-Leu | 8 | |
PropeptideiPRO_0000008249 | 196 – 207 | Add BLAST | 12 | |
PeptideiPRO_0000008250 | 210 – 214 | Met-enkephalin | 5 | |
PropeptideiPRO_0000008251 | 217 – 227 | Add BLAST | 11 | |
PeptideiPRO_0000008252 | 230 – 234 | Leu-enkephalin | 5 | |
PeptideiPRO_0000377693 | 237 – 258 | PENK(237-258)By similarityAdd BLAST | 22 | |
PeptideiPRO_0000008254 | 261 – 267 | Met-enkephalin-Arg-Phe | 7 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 26 ↔ 48 | 1 Publication | ||
Disulfide bondi | 30 ↔ 52 | 1 Publication | ||
Disulfide bondi | 33 ↔ 65 | 1 Publication | ||
Modified residuei | 251 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 111 – 112 | Cleavage; by CTSLBy similarity | 2 | |
Sitei | 112 – 113 | Cleavage; by CTSLBy similarity | 2 | |
Sitei | 133 – 134 | Cleavage; by CTSLBy similarity | 2 | |
Sitei | 214 – 215 | Cleavage; by CTSLBy similarity | 2 | |
Sitei | 215 – 216 | Cleavage; by CTSLBy similarity | 2 | |
Sitei | 218 – 219 | Cleavage; by CTSLBy similarity | 2 |
Keywords - PTMi
Cleavage on pair of basic residues, Disulfide bond, PhosphoproteinProteomic databases
jPOSTi | P01210 |
MassIVEi | P01210 |
PaxDbi | P01210 |
PeptideAtlasi | P01210 |
PRIDEi | P01210 |
ProteomicsDBi | 51344 |
PTM databases
iPTMneti | P01210 |
PhosphoSitePlusi | P01210 |
Expressioni
Gene expression databases
Bgeei | ENSG00000181195, Expressed in caudate nucleus and 205 other tissues |
ExpressionAtlasi | P01210, baseline and differential |
Genevisiblei | P01210, HS |
Organism-specific databases
HPAi | ENSG00000181195, Group enriched (adrenal gland, brain, testis) |
Interactioni
Binary interactionsi
GO - Molecular functioni
- neuropeptide hormone activity Source: ProtInc
- opioid peptide activity Source: UniProtKB-KW
- opioid receptor binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 111205, 8 interactors |
IntActi | P01210, 9 interactors |
STRINGi | 9606.ENSP00000324248 |
Miscellaneous databases
RNActi | P01210, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P01210 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P01210 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 163 – 182 | DisorderedSequence analysisAdd BLAST | 20 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG502QWWK, Eukaryota |
GeneTreei | ENSGT00950000183149 |
HOGENOMi | CLU_070973_0_0_1 |
InParanoidi | P01210 |
OMAi | DELYPVE |
OrthoDBi | 1149395at2759 |
PhylomeDBi | P01210 |
TreeFami | TF332620 |
Family and domain databases
InterProi | View protein in InterPro IPR006024, Opioid_neupept IPR000703, Proenkphlin_A |
PANTHERi | PTHR11438, PTHR11438, 1 hit PTHR11438:SF3, PTHR11438:SF3, 1 hit |
Pfami | View protein in Pfam PF01160, Opiods_neuropep, 1 hit |
PRINTSi | PR01028, OPIOIDPRCRSR PR01029, PENKAPRCRSR |
PROSITEi | View protein in PROSITE PS01252, OPIOIDS_PRECURSOR, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MARFLTLCTW LLLLGPGLLA TVRAECSQDC ATCSYRLVRP ADINFLACVM
60 70 80 90 100
ECEGKLPSLK IWETCKELLQ LSKPELPQDG TSTLRENSKP EESHLLAKRY
110 120 130 140 150
GGFMKRYGGF MKKMDELYPM EPEEEANGSE ILAKRYGGFM KKDAEEDDSL
160 170 180 190 200
ANSSDLLKEL LETGDNRERS HHQDGSDNEE EVSKRYGGFM RGLKRSPQLE
210 220 230 240 250
DEAKELQKRY GGFMRRVGRP EWWMDYQKRY GGFLKRFAEA LPSDEEGESY
260
SKEVPEMEKR YGGFMRF
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE5RFR1 | E5RFR1_HUMAN | Proenkephalin-A | PENK | 105 | Annotation score: | ||
E5RIP6 | E5RIP6_HUMAN | Proenkephalin-A | PENK | 79 | Annotation score: | ||
E5RJ72 | E5RJ72_HUMAN | Proenkephalin-A | PENK | 92 | Annotation score: | ||
H0YBT5 | H0YBT5_HUMAN | Proenkephalin-A | PENK | 88 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 119 | P → S in CAG46627 (Ref. 4) Curated | 1 | |
Sequence conflicti | 152 | N → I in CAG46607 (Ref. 4) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_048935 | 83 | T → N. Corresponds to variant dbSNP:rs11998459EnsemblClinVar. | 1 | |
Natural variantiVAR_014584 | 247 | G → D. Corresponds to variant dbSNP:rs1800567Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V00509 Genomic DNA Translation: CAA23767.1 J00123, J00122 Genomic DNA Translation: AAB59409.1 AK314908 mRNA Translation: BAG37420.1 CR541808 mRNA Translation: CAG46607.1 CR541828 mRNA Translation: CAG46627.1 CH471068 Genomic DNA Translation: EAW86785.1 BC032505 mRNA Translation: AAH32505.1 |
CCDSi | CCDS6168.1 |
PIRi | A93278, EQHUA |
RefSeqi | NP_001129162.1, NM_001135690.2 |
Genome annotation databases
Ensembli | ENST00000314922; ENSP00000324248; ENSG00000181195 ENST00000451791; ENSP00000400894; ENSG00000181195 |
GeneIDi | 5179 |
KEGGi | hsa:5179 |
MANE-Selecti | ENST00000451791.7; ENSP00000400894.2; NM_001135690.3; NP_001129162.1 |
UCSCi | uc003xsz.3, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V00509 Genomic DNA Translation: CAA23767.1 J00123, J00122 Genomic DNA Translation: AAB59409.1 AK314908 mRNA Translation: BAG37420.1 CR541808 mRNA Translation: CAG46607.1 CR541828 mRNA Translation: CAG46627.1 CH471068 Genomic DNA Translation: EAW86785.1 BC032505 mRNA Translation: AAH32505.1 |
CCDSi | CCDS6168.1 |
PIRi | A93278, EQHUA |
RefSeqi | NP_001129162.1, NM_001135690.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1PLW | NMR | - | A | 100-104 | [»] | |
1PLX | NMR | - | A | 100-104 | [»] | |
2LWC | NMR | - | A | 261-265 | [»] | |
5E33 | X-ray | 1.84 | B | 261-265 | [»] | |
5E3A | X-ray | 2.05 | B | 230-234 | [»] | |
SMRi | P01210 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111205, 8 interactors |
IntActi | P01210, 9 interactors |
STRINGi | 9606.ENSP00000324248 |
Protein family/group databases
TCDBi | 1.C.89.1.2, the dynorphin channel-forming neuropeptide (dynorphin) family |
PTM databases
iPTMneti | P01210 |
PhosphoSitePlusi | P01210 |
Genetic variation databases
BioMutai | PENK |
DMDMi | 129770 |
Proteomic databases
jPOSTi | P01210 |
MassIVEi | P01210 |
PaxDbi | P01210 |
PeptideAtlasi | P01210 |
PRIDEi | P01210 |
ProteomicsDBi | 51344 |
Protocols and materials databases
Antibodypediai | 2211, 394 antibodies from 36 providers |
DNASUi | 5179 |
Genome annotation databases
Ensembli | ENST00000314922; ENSP00000324248; ENSG00000181195 ENST00000451791; ENSP00000400894; ENSG00000181195 |
GeneIDi | 5179 |
KEGGi | hsa:5179 |
MANE-Selecti | ENST00000451791.7; ENSP00000400894.2; NM_001135690.3; NP_001129162.1 |
UCSCi | uc003xsz.3, human |
Organism-specific databases
CTDi | 5179 |
DisGeNETi | 5179 |
GeneCardsi | PENK |
HGNCi | HGNC:8831, PENK |
HPAi | ENSG00000181195, Group enriched (adrenal gland, brain, testis) |
MIMi | 131330, gene |
neXtProti | NX_P01210 |
OpenTargetsi | ENSG00000181195 |
PharmGKBi | PA33176 |
VEuPathDBi | HostDB:ENSG00000181195 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QWWK, Eukaryota |
GeneTreei | ENSGT00950000183149 |
HOGENOMi | CLU_070973_0_0_1 |
InParanoidi | P01210 |
OMAi | DELYPVE |
OrthoDBi | 1149395at2759 |
PhylomeDBi | P01210 |
TreeFami | TF332620 |
Enzyme and pathway databases
PathwayCommonsi | P01210 |
Reactomei | R-HSA-375276, Peptide ligand-binding receptors R-HSA-381426, Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) R-HSA-418594, G alpha (i) signalling events R-HSA-8957275, Post-translational protein phosphorylation |
SignaLinki | P01210 |
SIGNORi | P01210 |
Miscellaneous databases
BioGRID-ORCSi | 5179, 1 hit in 1032 CRISPR screens |
ChiTaRSi | PENK, human |
EvolutionaryTracei | P01210 |
GenomeRNAii | 5179 |
Pharosi | P01210, Tbio |
PROi | PR:P01210 |
RNActi | P01210, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000181195, Expressed in caudate nucleus and 205 other tissues |
ExpressionAtlasi | P01210, baseline and differential |
Genevisiblei | P01210, HS |
Family and domain databases
InterProi | View protein in InterPro IPR006024, Opioid_neupept IPR000703, Proenkphlin_A |
PANTHERi | PTHR11438, PTHR11438, 1 hit PTHR11438:SF3, PTHR11438:SF3, 1 hit |
Pfami | View protein in Pfam PF01160, Opiods_neuropep, 1 hit |
PRINTSi | PR01028, OPIOIDPRCRSR PR01029, PENKAPRCRSR |
PROSITEi | View protein in PROSITE PS01252, OPIOIDS_PRECURSOR, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PENK_HUMAN | |
Accessioni | P01210Primary (citable) accession number: P01210 Secondary accession number(s): B2RC23, Q6FHC6, Q6FHE6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | February 23, 2022 | |
This is version 183 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 8
Human chromosome 8: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families