UniProtKB - P01139 (NGF_MOUSE)
Beta-nerve growth factor
Ngf
Functioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 171 | a 1-acyl-sn-glycero-3-phospho-(1D-myo-inositol); via amide nitrogen and carbonyl oxygenCombined sources1 Publication | 1 | |
Binding sitei | 171 | a 1-acyl-sn-glycero-3-phospho-L-serine; via carbonyl oxygenCombined sources1 Publication | 1 | |
Binding sitei | 173 | a 1-acyl-sn-glycero-3-phospho-(1D-myo-inositol); via amide nitrogen and carbonyl oxygenCombined sources1 Publication | 1 | |
Binding sitei | 209 | a 1-acyl-sn-glycero-3-phospho-(1D-myo-inositol); shared with dimeric partnerCombined sources1 Publication | 1 | |
Binding sitei | 209 | a 1-acyl-sn-glycero-3-phospho-L-serine; shared with dimeric partnerCombined sources1 Publication | 1 |
GO - Molecular functioni
- cysteine-type endopeptidase activator activity involved in apoptotic process Source: MGI
- death receptor agonist activity Source: MGI
- growth factor activity Source: GO_Central
- lipid binding Source: UniProtKB-KW
- metalloendopeptidase inhibitor activity Source: UniProtKB
- nerve growth factor receptor binding Source: MGI
- transmembrane receptor protein tyrosine kinase activator activity Source: BHF-UCL
GO - Biological processi
- activation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
- adult locomotory behavior Source: MGI
- circadian rhythm Source: UniProtKB
- extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
- extrinsic apoptotic signaling pathway via death domain receptors Source: MGI
- memory Source: MGI
- modulation of chemical synaptic transmission Source: GO_Central
- negative regulation of neuron apoptotic process Source: MGI
- negative regulation of type B pancreatic cell apoptotic process Source: MGI
- nerve development Source: GO_Central
- nerve growth factor signaling pathway Source: MGI
- neuron apoptotic process Source: MGI
- neuron development Source: MGI
- neuron projection development Source: MGI
- neuron projection morphogenesis Source: MGI
- peripheral nervous system development Source: MGI
- positive regulation of axon extension Source: MGI
- positive regulation of cell growth Source: MGI
- positive regulation of cell population proliferation Source: MGI
- positive regulation of collateral sprouting Source: MGI
- positive regulation of DNA binding Source: ARUK-UCL
- positive regulation of DNA-binding transcription factor activity Source: MGI
- positive regulation of ERK1 and ERK2 cascade Source: MGI
- positive regulation of gene expression Source: ARUK-UCL
- positive regulation of neuron differentiation Source: MGI
- positive regulation of neuron maturation Source: MGI
- positive regulation of neuron projection development Source: MGI
- positive regulation of neurotrophin TRK receptor signaling pathway Source: MGI
- positive regulation of peptidyl-serine phosphorylation Source: GO_Central
- positive regulation of protein autophosphorylation Source: MGI
- positive regulation of protein binding Source: MGI
- positive regulation of protein phosphorylation Source: BHF-UCL
- positive regulation of protein ubiquitination Source: MGI
- positive regulation of Ras protein signal transduction Source: ParkinsonsUK-UCL
- positive regulation of stem cell proliferation Source: MGI
- regulation of neuron differentiation Source: MGI
- regulation of neurotransmitter secretion Source: MGI
- regulation of release of sequestered calcium ion into cytosol Source: MGI
- sensory perception of pain Source: MGI
- transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
Keywordsi
Molecular function | Growth factor, Metalloenzyme inhibitor, Metalloprotease inhibitor, Protease inhibitor |
Ligand | Lipid-binding |
Enzyme and pathway databases
Reactomei | R-MMU-167060, NGF processing R-MMU-170968, Frs2-mediated activation R-MMU-170984, ARMS-mediated activation R-MMU-177504, Retrograde neurotrophin signalling R-MMU-187042, TRKA activation by NGF R-MMU-198203, PI3K/AKT activation R-MMU-205017, NFG and proNGF binds to p75NTR R-MMU-205025, NADE modulates death signalling R-MMU-205043, NRIF signals cell death from the nucleus R-MMU-209543, p75NTR recruits signalling complexes R-MMU-209560, NF-kB is activated and signals survival R-MMU-209563, Axonal growth stimulation |
Names & Taxonomyi
Protein namesi | Recommended name: Beta-nerve growth factorShort name: Beta-NGF |
Gene namesi | Name:Ngf Synonyms:Ngfb |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:97321, Ngf |
Subcellular locationi
Extracellular region or secreted
- Secreted 1 Publication
Endosome
- Endosome lumen 1 Publication
Note: ProNGF is endocytosed after binding to the cell surface receptor formed by SORT1 and NGFR.1 Publication
Endoplasmic reticulum
- endoplasmic reticulum lumen Source: Reactome
Endosome
- endosome lumen Source: UniProtKB-SubCell
Extracellular region or secreted
- extracellular region Source: Reactome
- extracellular space Source: BHF-UCL
Other locations
- axon Source: GO_Central
- cytoplasmic vesicle Source: GO_Central
- dendrite Source: GO_Central
- neuron projection terminus Source: MGI
- synaptic vesicle Source: GO_Central
Keywords - Cellular componenti
Endosome, SecretedPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 49 – 50 | RR → AA: Abolishes production of the mature chain and promotes apoptosis of superior cervical ganglion neurons; when associated with 79-A-A-80 and 120-A-A-121. 1 Publication | 2 | |
Mutagenesisi | 79 – 80 | KR → AA: Abolishes production of the mature chain and promotes apoptosis of superior cervical ganglion neurons; when associated with 49-A-A-50 and 120-A-A-121. 1 Publication | 2 | |
Mutagenesisi | 120 – 121 | KR → AA: Abolishes production of the mature chain and promotes apoptosis of superior cervical ganglion neurons; when associated with 49-A-A-50 and 79-A-A-80. 1 Publication | 2 | |
Mutagenesisi | 209 | K → L: Near loss of the ability to trigger histamine release from mast cells. No effect on interaction with NTRK1. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | 1 PublicationAdd BLAST | 18 | |
PropeptideiPRO_0000019601 | 19 – 121 | 2 PublicationsAdd BLAST | 103 | |
ChainiPRO_0000019602 | 122 – 241 | Beta-nerve growth factorAdd BLAST | 120 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 69 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 114 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 136 ↔ 201 | Combined sources5 Publications | ||
Disulfide bondi | 179 ↔ 229 | Combined sources5 Publications | ||
Disulfide bondi | 189 ↔ 231 | Combined sources5 Publications |
Keywords - PTMi
Cleavage on pair of basic residues, Disulfide bond, GlycoproteinProteomic databases
MaxQBi | P01139 |
PaxDbi | P01139 |
PeptideAtlasi | P01139 |
PRIDEi | P01139 |
PTM databases
GlyGeni | P01139, 2 sites |
PhosphoSitePlusi | P01139 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000027859, Expressed in submandibular gland and 88 other tissues |
Genevisiblei | P01139, MM |
Interactioni
Subunit structurei
Homodimer (PubMed:1956407, PubMed:8201620, PubMed:20036257, PubMed:22649032, PubMed:26144237, PubMed:30061605). The homodimer interacts with a single NTRK1 chain (PubMed:22649032). The homodimer interacts with a single NGFR chain (By similarity). The NGF dimer interacts with a single SORCS2 chain (via extracellular domain) (PubMed:30061605). The NGF precursor (proNGF) binds to a receptor complex formed by SORT1 and NGFR, which leads to NGF endocytosis (PubMed:20036257). Both mature NGF and the immature NGF precursor (proNGF) interact with SORCS2 and with the heterodimer formed by SORCS2 and NGFR (via extracellular domains) (PubMed:22155786, PubMed:30061605). The NGF precursor (proNGF) has much higher affinity for SORCS2 than mature NGF (PubMed:24908487). The NGF precursor (proNGF) has much higher affinity for SORT1 than mature NGF (PubMed:20036257).
Interacts with ADAM10 in a divalent cation-dependent manner (By similarity).
By similarity8 PublicationsGO - Molecular functioni
- death receptor agonist activity Source: MGI
- growth factor activity Source: GO_Central
- nerve growth factor receptor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 201764, 3 interactors |
DIPi | DIP-59840N |
IntActi | P01139, 4 interactors |
STRINGi | 10090.ENSMUSP00000102538 |
Miscellaneous databases
RNActi | P01139, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P01139 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P01139 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG502RYPU, Eukaryota |
GeneTreei | ENSGT00390000007725 |
HOGENOMi | CLU_059942_1_1_1 |
InParanoidi | P01139 |
OrthoDBi | 1156054at2759 |
PhylomeDBi | P01139 |
Family and domain databases
DisProti | DP00836 |
Gene3Di | 2.10.90.10, 1 hit |
InterProi | View protein in InterPro IPR029034, Cystine-knot_cytokine IPR020408, Nerve_growth_factor-like IPR002072, Nerve_growth_factor-rel IPR020425, Nerve_growth_factor_bsu IPR020437, Nerve_growth_factor_bsu_mml IPR019846, Nerve_growth_factor_CS |
PANTHERi | PTHR11589, PTHR11589, 1 hit |
Pfami | View protein in Pfam PF00243, NGF, 1 hit |
PIRSFi | PIRSF001789, NGF, 1 hit |
PRINTSi | PR01925, MAMLNGFBETA PR00268, NGF PR01913, NGFBETA |
SMARTi | View protein in SMART SM00140, NGF, 1 hit |
SUPFAMi | SSF57501, SSF57501, 1 hit |
PROSITEi | View protein in PROSITE PS00248, NGF_1, 1 hit PS50270, NGF_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MSMLFYTLIT AFLIGVQAEP YTDSNVPEGD SVPEAHWTKL QHSLDTALRR
60 70 80 90 100
ARSAPTAPIA ARVTGQTRNI TVDPRLFKKR RLHSPRVLFS TQPPPTSSDT
110 120 130 140 150
LDLDFQAHGT IPFNRTHRSK RSSTHPVFHM GEFSVCDSVS VWVGDKTTAT
160 170 180 190 200
DIKGKEVTVL AEVNINNSVF RQYFFETKCR ASNPVESGCR GIDSKHWNSY
210 220 230 240
CTTTHTFVKA LTTDEKQAAW RFIRIDTACV CVLSRKATRR G
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ6LDU8 | Q6LDU8_MOUSE | Beta-nerve growth factor | Ngf NGF, Ngfb | 307 | Annotation score: | ||
A0A0G2JEK0 | A0A0G2JEK0_MOUSE | Beta-nerve growth factor | Ngf | 303 | Annotation score: | ||
A0A0G2JG51 | A0A0G2JG51_MOUSE | Beta-nerve growth factor | Ngf | 22 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 233 – 241 | LSRKATRRG → CSAGRLQEEADLPAAPFPTC PLHTLLGPSLPQPVNYFKL in AAB26820 (PubMed:1284621).Curated | 9 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M35075 mRNA Translation: AAA39818.1 Different initiation. V00836 mRNA Translation: CAA24221.1 Different initiation. K01759 mRNA Translation: AAA39820.1 Different initiation. M14805 mRNA Translation: AAA39821.1 Different initiation. M17298, M17296, M17297 Genomic DNA Translation: AAA37687.1 Different initiation. S62089 mRNA Translation: AAB26820.2 |
CCDSi | CCDS51025.1 |
RefSeqi | NP_001106168.1, NM_001112698.2 NP_038637.1, NM_013609.3 XP_006501171.1, XM_006501108.3 |
Genome annotation databases
Ensembli | ENSMUST00000035952; ENSMUSP00000040345; ENSMUSG00000027859 |
GeneIDi | 18049 |
KEGGi | mmu:18049 |
UCSCi | uc012cuz.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M35075 mRNA Translation: AAA39818.1 Different initiation. V00836 mRNA Translation: CAA24221.1 Different initiation. K01759 mRNA Translation: AAA39820.1 Different initiation. M14805 mRNA Translation: AAA39821.1 Different initiation. M17298, M17296, M17297 Genomic DNA Translation: AAA37687.1 Different initiation. S62089 mRNA Translation: AAB26820.2 |
CCDSi | CCDS51025.1 |
RefSeqi | NP_001106168.1, NM_001112698.2 NP_038637.1, NM_013609.3 XP_006501171.1, XM_006501108.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1BET | X-ray | 2.30 | A | 131-237 | [»] | |
1BTG | X-ray | 2.50 | A/B/C | 130-239 | [»] | |
1SGF | X-ray | 3.15 | B/Y | 122-239 | [»] | |
3IJ2 | X-ray | 3.75 | A/B | 19-241 | [»] | |
4EAX | X-ray | 2.30 | A/B/C/D | 122-241 | [»] | |
4XPJ | X-ray | 2.60 | A/B | 122-241 | [»] | |
5LSD | NMR | - | A/B | 122-239 | [»] | |
6FFY | X-ray | 3.90 | B/C | 122-238 | [»] | |
SMRi | P01139 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 201764, 3 interactors |
DIPi | DIP-59840N |
IntActi | P01139, 4 interactors |
STRINGi | 10090.ENSMUSP00000102538 |
PTM databases
GlyGeni | P01139, 2 sites |
PhosphoSitePlusi | P01139 |
Proteomic databases
MaxQBi | P01139 |
PaxDbi | P01139 |
PeptideAtlasi | P01139 |
PRIDEi | P01139 |
Protocols and materials databases
ABCDi | P01139, 51 sequenced antibodies |
Antibodypediai | 20173, 1058 antibodies |
DNASUi | 18049 |
Genome annotation databases
Ensembli | ENSMUST00000035952; ENSMUSP00000040345; ENSMUSG00000027859 |
GeneIDi | 18049 |
KEGGi | mmu:18049 |
UCSCi | uc012cuz.2, mouse |
Organism-specific databases
CTDi | 4803 |
MGIi | MGI:97321, Ngf |
Phylogenomic databases
eggNOGi | ENOG502RYPU, Eukaryota |
GeneTreei | ENSGT00390000007725 |
HOGENOMi | CLU_059942_1_1_1 |
InParanoidi | P01139 |
OrthoDBi | 1156054at2759 |
PhylomeDBi | P01139 |
Enzyme and pathway databases
Reactomei | R-MMU-167060, NGF processing R-MMU-170968, Frs2-mediated activation R-MMU-170984, ARMS-mediated activation R-MMU-177504, Retrograde neurotrophin signalling R-MMU-187042, TRKA activation by NGF R-MMU-198203, PI3K/AKT activation R-MMU-205017, NFG and proNGF binds to p75NTR R-MMU-205025, NADE modulates death signalling R-MMU-205043, NRIF signals cell death from the nucleus R-MMU-209543, p75NTR recruits signalling complexes R-MMU-209560, NF-kB is activated and signals survival R-MMU-209563, Axonal growth stimulation |
Miscellaneous databases
BioGRID-ORCSi | 18049, 0 hits in 18 CRISPR screens |
EvolutionaryTracei | P01139 |
PROi | PR:P01139 |
RNActi | P01139, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027859, Expressed in submandibular gland and 88 other tissues |
Genevisiblei | P01139, MM |
Family and domain databases
DisProti | DP00836 |
Gene3Di | 2.10.90.10, 1 hit |
InterProi | View protein in InterPro IPR029034, Cystine-knot_cytokine IPR020408, Nerve_growth_factor-like IPR002072, Nerve_growth_factor-rel IPR020425, Nerve_growth_factor_bsu IPR020437, Nerve_growth_factor_bsu_mml IPR019846, Nerve_growth_factor_CS |
PANTHERi | PTHR11589, PTHR11589, 1 hit |
Pfami | View protein in Pfam PF00243, NGF, 1 hit |
PIRSFi | PIRSF001789, NGF, 1 hit |
PRINTSi | PR01925, MAMLNGFBETA PR00268, NGF PR01913, NGFBETA |
SMARTi | View protein in SMART SM00140, NGF, 1 hit |
SUPFAMi | SSF57501, SSF57501, 1 hit |
PROSITEi | View protein in PROSITE PS00248, NGF_1, 1 hit PS50270, NGF_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NGF_MOUSE | |
Accessioni | P01139Primary (citable) accession number: P01139 Secondary accession number(s): Q63864, Q6LDB7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | January 1, 1990 | |
Last modified: | February 10, 2021 | |
This is version 192 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families