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Protein

Pro-epidermal growth factor

Gene

Egf

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6 (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGrowth factor

Enzyme and pathway databases

ReactomeiR-MMU-114608 Platelet degranulation
R-MMU-1227986 Signaling by ERBB2
R-MMU-1236394 Signaling by ERBB4
R-MMU-1250196 SHC1 events in ERBB2 signaling
R-MMU-1257604 PIP3 activates AKT signaling
R-MMU-177929 Signaling by EGFR
R-MMU-179812 GRB2 events in EGFR signaling
R-MMU-180292 GAB1 signalosome
R-MMU-180336 SHC1 events in EGFR signaling
R-MMU-182971 EGFR downregulation
R-MMU-1963642 PI3K events in ERBB2 signaling
R-MMU-212718 EGFR interacts with phospholipase C-gamma
R-MMU-5673001 RAF/MAP kinase cascade
R-MMU-6785631 ERBB2 Regulates Cell Motility
R-MMU-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling
R-MMU-8847993 ERBB2 Activates PTK6 Signaling
R-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis
R-MMU-8856828 Clathrin-mediated endocytosis
R-MMU-8863795 Downregulation of ERBB2 signaling
R-MMU-9013507 NOTCH3 Activation and Transmission of Signal to the Nucleus

Names & Taxonomyi

Protein namesi
Recommended name:
Pro-epidermal growth factor
Short name:
EGF
Cleaved into the following chain:
Gene namesi
Name:Egf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:95290 Egf

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini29 – 1038ExtracellularSequence analysisAdd BLAST1010
Transmembranei1039 – 1058HelicalSequence analysisAdd BLAST20
Topological domaini1059 – 1217CytoplasmicSequence analysisAdd BLAST159

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Sequence analysisAdd BLAST28
ChainiPRO_000000754229 – 1217Pro-epidermal growth factorAdd BLAST1189
ChainiPRO_0000007543977 – 1029Epidermal growth factor1 PublicationAdd BLAST53

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi111N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi366 ↔ 377PROSITE-ProRule annotation
Disulfide bondi373 ↔ 386PROSITE-ProRule annotation
Disulfide bondi388 ↔ 401PROSITE-ProRule annotation
Disulfide bondi407 ↔ 418PROSITE-ProRule annotation
Glycosylationi410N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi414 ↔ 427PROSITE-ProRule annotation
Disulfide bondi429 ↔ 442PROSITE-ProRule annotation
Disulfide bondi445 ↔ 457PROSITE-ProRule annotation
Disulfide bondi453 ↔ 467PROSITE-ProRule annotation
Disulfide bondi469 ↔ 482PROSITE-ProRule annotation
Disulfide bondi751 ↔ 762PROSITE-ProRule annotation
Disulfide bondi758 ↔ 771PROSITE-ProRule annotation
Disulfide bondi773 ↔ 786PROSITE-ProRule annotation
Glycosylationi810N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi842 ↔ 853PROSITE-ProRule annotation
Disulfide bondi847 ↔ 862PROSITE-ProRule annotation
Disulfide bondi864 ↔ 875PROSITE-ProRule annotation
Disulfide bondi881 ↔ 895PROSITE-ProRule annotation
Disulfide bondi888 ↔ 904PROSITE-ProRule annotation
Disulfide bondi906 ↔ 917PROSITE-ProRule annotation
Disulfide bondi923 ↔ 936PROSITE-ProRule annotation
Disulfide bondi930 ↔ 945PROSITE-ProRule annotation
Glycosylationi944N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi947 ↔ 958PROSITE-ProRule annotation
Disulfide bondi982 ↔ 996PROSITE-ProRule annotation1 Publication
Disulfide bondi990 ↔ 1007PROSITE-ProRule annotation1 Publication
Disulfide bondi1009 ↔ 1018PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP01132
PaxDbiP01132
PeptideAtlasiP01132
PRIDEiP01132

PTM databases

PhosphoSitePlusiP01132

Miscellaneous databases

PMAP-CutDBiP01132

Expressioni

Gene expression databases

BgeeiENSMUSG00000028017 Expressed in 136 organ(s), highest expression level in submandibular gland
CleanExiMM_EGF
ExpressionAtlasiP01132 baseline and differential
GenevisibleiP01132 MM

Interactioni

Subunit structurei

Interacts with EGFR and promotes EGFR dimerization. Interacts with RHBDF1; may retain EGF in the endoplasmic reticulum and regulates its degradation through the endoplasmic reticulum-associated degradation (ERAD). Interacts with RHBDF2.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-5762N
IntActiP01132, 5 interactors
MINTiP01132
STRINGi10090.ENSMUSP00000029653

Structurei

Secondary structure

11217
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP01132
SMRiP01132
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP01132

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati93 – 134LDL-receptor class B 1Add BLAST42
Repeati135 – 176LDL-receptor class B 2Add BLAST42
Repeati177 – 219LDL-receptor class B 3Add BLAST43
Domaini327 – 361EGF-like 1; incompletePROSITE-ProRule annotationAdd BLAST35
Domaini362 – 402EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd BLAST41
Domaini403 – 443EGF-like 3PROSITE-ProRule annotationAdd BLAST41
Domaini441 – 483EGF-like 4PROSITE-ProRule annotationAdd BLAST43
Repeati489 – 529LDL-receptor class B 4Add BLAST41
Repeati530 – 572LDL-receptor class B 5Add BLAST43
Repeati573 – 615LDL-receptor class B 6Add BLAST43
Repeati616 – 659LDL-receptor class B 7Add BLAST44
Repeati660 – 702LDL-receptor class B 8Add BLAST43
Domaini747 – 787EGF-like 5PROSITE-ProRule annotationAdd BLAST41
Domaini838 – 876EGF-like 6PROSITE-ProRule annotationAdd BLAST39
Domaini877 – 918EGF-like 7; calcium-bindingPROSITE-ProRule annotationAdd BLAST42
Domaini919 – 959EGF-like 8; calcium-bindingPROSITE-ProRule annotationAdd BLAST41
Domaini978 – 1019EGF-like 9PROSITE-ProRule annotationAdd BLAST42

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1024 – 1029Not required for full biological activity6

Keywords - Domaini

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IPSY Eukaryota
ENOG410ZVIM LUCA
GeneTreeiENSGT00910000144086
HOGENOMiHOG000112345
HOVERGENiHBG003858
InParanoidiP01132
KOiK04357
OMAiKIYFAHT
OrthoDBiEOG091G014V
TreeFamiTF315253

Family and domain databases

Gene3Di2.120.10.30, 2 hits
InterProiView protein in InterPro
IPR011042 6-blade_b-propeller_TolB-like
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000152 EGF-type_Asp/Asn_hydroxyl_site
IPR018097 EGF_Ca-bd_CS
IPR009030 Growth_fac_rcpt_cys_sf
IPR000033 LDLR_classB_rpt
IPR016317 Pro-epidermal_GF
PfamiView protein in Pfam
PF00008 EGF, 1 hit
PF07645 EGF_CA, 3 hits
PF00058 Ldl_recept_b, 3 hits
PIRSFiPIRSF001778 Pro-epidermal_growth_factor, 1 hit
SMARTiView protein in SMART
SM00181 EGF, 9 hits
SM00179 EGF_CA, 8 hits
SM00135 LY, 9 hits
SUPFAMiSSF57184 SSF57184, 2 hits
PROSITEiView protein in PROSITE
PS00010 ASX_HYDROXYL, 3 hits
PS00022 EGF_1, 1 hit
PS01186 EGF_2, 6 hits
PS50026 EGF_3, 5 hits
PS01187 EGF_CA, 3 hits
PS51120 LDLRB, 8 hits

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.iShow all

P01132-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPWGRRPTWL LLAFLLVFLK ISILSVTAWQ TGNCQPGPLE RSERSGTCAG
60 70 80 90 100
PAPFLVFSQG KSISRIDPDG TNHQQLVVDA GISADMDIHY KKERLYWVDV
110 120 130 140 150
ERQVLLRVFL NGTGLEKVCN VERKVSGLAI DWIDDEVLWV DQQNGVITVT
160 170 180 190 200
DMTGKNSRVL LSSLKHPSNI AVDPIERLMF WSSEVTGSLH RAHLKGVDVK
210 220 230 240 250
TLLETGGISV LTLDVLDKRL FWVQDSGEGS HAYIHSCDYE GGSVRLIRHQ
260 270 280 290 300
ARHSLSSMAF FGDRIFYSVL KSKAIWIANK HTGKDTVRIN LHPSFVTPGK
310 320 330 340 350
LMVVHPRAQP RTEDAAKDPD PELLKQRGRP CRFGLCERDP KSHSSACAEG
360 370 380 390 400
YTLSRDRKYC EDVNECATQN HGCTLGCENT PGSYHCTCPT GFVLLPDGKQ
410 420 430 440 450
CHELVSCPGN VSKCSHGCVL TSDGPRCICP AGSVLGRDGK TCTGCSSPDN
460 470 480 490 500
GGCSQICLPL RPGSWECDCF PGYDLQSDRK SCAASGPQPL LLFANSQDIR
510 520 530 540 550
HMHFDGTDYK VLLSRQMGMV FALDYDPVES KIYFAQTALK WIERANMDGS
560 570 580 590 600
QRERLITEGV DTLEGLALDW IGRRIYWTDS GKSVVGGSDL SGKHHRIIIQ
610 620 630 640 650
ERISRPRGIA VHPRARRLFW TDVGMSPRIE SASLQGSDRV LIASSNLLEP
660 670 680 690 700
SGITIDYLTD TLYWCDTKRS VIEMANLDGS KRRRLIQNDV GHPFSLAVFE
710 720 730 740 750
DHLWVSDWAI PSVIRVNKRT GQNRVRLQGS MLKPSSLVVV HPLAKPGADP
760 770 780 790 800
CLYRNGGCEH ICQESLGTAR CLCREGFVKA WDGKMCLPQD YPILSGENAD
810 820 830 840 850
LSKEVTSLSN STQAEVPDDD GTESSTLVAE IMVSGMNYED DCGPGGCGSH
860 870 880 890 900
ARCVSDGETA ECQCLKGFAR DGNLCSDIDE CVLARSDCPS TSSRCINTEG
910 920 930 940 950
GYVCRCSEGY EGDGISCFDI DECQRGAHNC GENAACTNTE GGYNCTCAGR
960 970 980 990 1000
PSSPGLSCPD STAPSLLGED GHHLDRNSYP GCPSSYDGYC LNGGVCMHIE
1010 1020 1030 1040 1050
SLDSYTCNCV IGYSGDRCQT RDLRWWELRH AGYGQKHDIM VVAVCMVALV
1060 1070 1080 1090 1100
LLLVLGMWGT YYYRTRKQLS NPPKNPCDEP SGSVSSSGPN SSSGAAVASC
1110 1120 1130 1140 1150
PQPWFVVLEK HQDPKNGSLP ADGTNGAVVD AGLSPSLQLG SVHLTSWRQK
1160 1170 1180 1190 1200
PHIDGMGTGQ SCWIPPSSDR GPQEIEGNSH LPSYRPVGPE KLHSLQSANG
1210
SCHERAPDLP RQTEPVQ
Length:1,217
Mass (Da):133,072
Last modified:July 27, 2011 - v2
Checksum:i28F35C928280D31B
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0G2JF92A0A0G2JF92_MOUSE
Pro-epidermal growth factor
Egf
716Annotation score:
A0A0G2JDT8A0A0G2JDT8_MOUSE
Pro-epidermal growth factor
Egf
675Annotation score:
A0A0G2JFB8A0A0G2JFB8_MOUSE
Pro-epidermal growth factor
Egf
254Annotation score:

Sequence cautioni

The sequence CAA24115 differs from that shown. Reason: Frameshift at positions 1134 and 1168.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti790D → Y in CAA24115 (PubMed:6304537).Curated1
Sequence conflicti931G → A in AAA37539 (PubMed:6602382).Curated1
Sequence conflicti931G → A in CAA24115 (PubMed:6304537).Curated1
Sequence conflicti931G → A in AAH60741 (PubMed:15489334).Curated1
Sequence conflicti931G → A in AAH92277 (PubMed:15489334).Curated1
Sequence conflicti956L → R in AAA37539 (PubMed:6602382).Curated1
Sequence conflicti956L → R in CAA24115 (PubMed:6304537).Curated1
Sequence conflicti956L → R in AAH60741 (PubMed:15489334).Curated1
Sequence conflicti956L → R in AAH92277 (PubMed:15489334).Curated1
Sequence conflicti1048A → S in CAA24115 (PubMed:6304537).Curated1
Sequence conflicti1054V → L in AAA37539 (PubMed:6602382).Curated1
Sequence conflicti1054V → L in CAA24115 (PubMed:6304537).Curated1
Sequence conflicti1054V → L in AAH60741 (PubMed:15489334).Curated1
Sequence conflicti1054V → L in AAH92277 (PubMed:15489334).Curated1
Sequence conflicti1090N → D in AAA37539 (PubMed:6602382).Curated1
Sequence conflicti1090N → D in CAA24115 (PubMed:6304537).Curated1
Sequence conflicti1090N → D in AAH60741 (PubMed:15489334).Curated1
Sequence conflicti1090N → D in AAH92277 (PubMed:15489334).Curated1
Sequence conflicti1217Q → K in AAA37539 (PubMed:6602382).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J00380 mRNA Translation: AAA37539.1
V00741 mRNA Translation: CAA24115.1 Frameshift.
V00741 mRNA Translation: CAA24116.1
AC098732 Genomic DNA No translation available.
BC060741 mRNA Translation: AAH60741.1
BC092277 mRNA Translation: AAH92277.1
CCDSiCCDS17833.1
RefSeqiNP_034243.2, NM_010113.4
UniGeneiMm.252481

Genome annotation databases

EnsembliENSMUST00000029653; ENSMUSP00000029653; ENSMUSG00000028017
GeneIDi13645
KEGGimmu:13645
UCSCiuc008rig.2 mouse

Similar proteinsi

Entry informationi

Entry nameiEGF_MOUSE
AccessioniPrimary (citable) accession number: P01132
Secondary accession number(s): E9QNX6, Q569W5, Q6P9J2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 27, 2011
Last modified: September 12, 2018
This is version 203 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health

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