UniProtKB - P01120 (RAS2_YEAST)
Protein
Ras-like protein 2
Gene
RAS2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
The S.cerevisiae Ras proteins modulate the activity of the adenylate cyclase catalytic subunit and therefore affect the biosynthesis of cyclic-AMP.
Miscellaneous
RAS2 is necessary for a normal response to nutrient limitations, in general, disruption of the RAS2 locus results in an overall premature starvation response.
Present with 19800 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:3.6.5.2By similarity
Activity regulationi
Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by guanine nucleotide-exchange factor (GEF) CDC25 and inactivated by GTPase-activating proteins (GAPs) IRA1 and IRA2.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 20 – 25 | GTPBy similarity | 6 | |
Nucleotide bindingi | 36 – 42 | GTPBy similarity | 7 | |
Nucleotide bindingi | 66 – 67 | GTPBy similarity | 2 | |
Nucleotide bindingi | 123 – 126 | GTPBy similarity | 4 | |
Nucleotide bindingi | 153 – 155 | GTPBy similarity | 3 |
GO - Molecular functioni
- GDP binding Source: GO_Central
- GTPase activity Source: SGD
- GTP binding Source: SGD
GO - Biological processi
- activation of adenylate cyclase activity Source: SGD
- ascospore formation Source: SGD
- cytoplasm to vacuole transport by the Cvt pathway Source: SGD
- macroautophagy Source: SGD
- positive regulation of adenylate cyclase activity Source: SGD
- positive regulation of pseudohyphal growth Source: SGD
- positive regulation of transcription by galactose Source: SGD
- protein localization to bud neck Source: SGD
- Ras protein signal transduction Source: GO_Central
- regulation of cytoplasmic mRNA processing body assembly Source: SGD
- regulation of protein localization Source: SGD
Keywordsi
Molecular function | Hydrolase |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-SCE-8873719, RAB geranylgeranylation |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:RAS2 Synonyms:ASC1, CTN5, GLC5 Ordered Locus Names:YNL098C ORF Names:N2198 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005042, RAS2 |
VEuPathDBi | FungiDB:YNL098C |
Subcellular locationi
Plasma membrane
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: SGD
Mitochondrion
- mitochondrion Source: SGD
Nucleus
- nucleus Source: SGD
Plasma Membrane
- plasma membrane Source: SGD
Other locations
- cell periphery Source: SGD
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 19 | G → V: Low sporulation efficiency. 1 Publication | 1 | |
Mutagenesisi | 70 | E → K in GLC5-1; low glycogen accumulation and sporulation deficiency. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000030195 | 2 – 319 | Ras-like protein 2Add BLAST | 318 | |
PropeptideiPRO_0000030196 | 320 – 322 | Removed in mature form1 Publication | 3 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 131 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources | ||
Modified residuei | 198 | PhosphoserineCombined sources | 1 | |
Modified residuei | 202 | PhosphoserineCombined sources | 1 | |
Modified residuei | 207 | PhosphoserineCombined sources | 1 | |
Modified residuei | 214 | PhosphoserineCombined sources | 1 | |
Modified residuei | 235 | PhosphoserineCombined sources | 1 | |
Modified residuei | 238 | PhosphoserineCombined sources | 1 | |
Lipidationi | 318 | S-palmitoyl cysteine1 Publication | 1 | |
Modified residuei | 319 | Cysteine methyl ester2 Publications | 1 | |
Lipidationi | 319 | S-farnesyl cysteine1 Publication | 1 |
Post-translational modificationi
Farnesylated by RAM1-RAM2, which is required for targeting RAS2 to the cytoplasmic site of the endoplasmic reticulum, where proteolytic processing of the C-terminus by RCE1 and methylation of the resulting carboxyl group by STE14 occurs.4 Publications
Palmitoylated by the ERF2-SHR5 complex, which is required for proper plasma membrane localization of RAS2.1 Publication
Keywords - PTMi
Isopeptide bond, Lipoprotein, Methylation, Palmitate, Phosphoprotein, Prenylation, Ubl conjugationProteomic databases
MaxQBi | P01120 |
PaxDbi | P01120 |
PRIDEi | P01120 |
PTM databases
iPTMneti | P01120 |
SwissPalmi | P01120 |
Interactioni
Binary interactionsi
Hide detailsP01120
With | #Exp. | IntAct |
---|---|---|
CDC25 [P04821] | 2 | EBI-14838,EBI-4237 |
LTE1 [P07866] | 5 | EBI-14838,EBI-10243 |
YCF1 [P39109] | 2 | EBI-14838,EBI-21779 |
RAF1 [P04049] from Homo sapiens. | 2 | EBI-14838,EBI-365996 |
Protein-protein interaction databases
BioGRIDi | 35725, 458 interactors |
DIPi | DIP-2263N |
IntActi | P01120, 16 interactors |
MINTi | P01120 |
STRINGi | 4932.YNL098C |
Chemistry databases
BindingDBi | P01120 |
Miscellaneous databases
RNActi | P01120, protein |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 39 – 47 | Effector region | 9 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0395, Eukaryota |
GeneTreei | ENSGT00940000176617 |
HOGENOMi | CLU_041217_9_0_1 |
InParanoidi | P01120 |
OMAi | WETSAKL |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase IPR020849, Small_GTPase_Ras-type |
PANTHERi | PTHR24070, PTHR24070, 1 hit |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51421, RAS, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P01120-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPLNKSNIRE YKLVVVGGGG VGKSALTIQL TQSHFVDEYD PTIEDSYRKQ
60 70 80 90 100
VVIDDEVSIL DILDTAGQEE YSAMREQYMR NGEGFLLVYS ITSKSSLDEL
110 120 130 140 150
MTYYQQILRV KDTDYVPIVV VGNKSDLENE KQVSYQDGLN MAKQMNAPFL
160 170 180 190 200
ETSAKQAINV EEAFYTLARL VRDEGGKYNK TLTENDNSKQ TSQDTKGSGA
210 220 230 240 250
NSVPRNSGGH RKMSNAANGK NVNSSTTVVN ARNASIESKT GLAGNQATNG
260 270 280 290 300
KTQTDRTNID NSTGQAGQAN AQSANTVNNR VNNNSKAGQV SNAKQARKQQ
310 320
AAPGGNTSEA SKSGSGGCCI IS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 108 | L → P in CAA25207 (PubMed:6328429).Curated | 1 | |
Sequence conflicti | 210 | H → L in CAA25207 (PubMed:6328429).Curated | 1 | |
Sequence conflicti | 255 | D → V in AAA34959 (PubMed:6365329).Curated | 1 | |
Sequence conflicti | 298 – 299 | KQ → SK in CAA25207 (PubMed:6328429).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K01971 Genomic DNA Translation: AAA34959.1 X00528 Genomic DNA Translation: CAA25207.1 D37950 Genomic DNA Translation: BAA22510.1 DQ115393 Genomic DNA Translation: AAZ22509.1 Z50161 Genomic DNA Translation: CAA90528.1 Z71374 Genomic DNA Translation: CAA95974.1 BK006947 Genomic DNA Translation: DAA10447.1 |
PIRi | S58254, TVBYR2 |
RefSeqi | NP_014301.1, NM_001182936.1 |
Genome annotation databases
EnsemblFungii | YNL098C_mRNA; YNL098C; YNL098C |
GeneIDi | 855625 |
KEGGi | sce:YNL098C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K01971 Genomic DNA Translation: AAA34959.1 X00528 Genomic DNA Translation: CAA25207.1 D37950 Genomic DNA Translation: BAA22510.1 DQ115393 Genomic DNA Translation: AAZ22509.1 Z50161 Genomic DNA Translation: CAA90528.1 Z71374 Genomic DNA Translation: CAA95974.1 BK006947 Genomic DNA Translation: DAA10447.1 |
PIRi | S58254, TVBYR2 |
RefSeqi | NP_014301.1, NM_001182936.1 |
3D structure databases
SMRi | P01120 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 35725, 458 interactors |
DIPi | DIP-2263N |
IntActi | P01120, 16 interactors |
MINTi | P01120 |
STRINGi | 4932.YNL098C |
Chemistry databases
BindingDBi | P01120 |
PTM databases
iPTMneti | P01120 |
SwissPalmi | P01120 |
Proteomic databases
MaxQBi | P01120 |
PaxDbi | P01120 |
PRIDEi | P01120 |
Genome annotation databases
EnsemblFungii | YNL098C_mRNA; YNL098C; YNL098C |
GeneIDi | 855625 |
KEGGi | sce:YNL098C |
Organism-specific databases
SGDi | S000005042, RAS2 |
VEuPathDBi | FungiDB:YNL098C |
Phylogenomic databases
eggNOGi | KOG0395, Eukaryota |
GeneTreei | ENSGT00940000176617 |
HOGENOMi | CLU_041217_9_0_1 |
InParanoidi | P01120 |
OMAi | WETSAKL |
Enzyme and pathway databases
Reactomei | R-SCE-8873719, RAB geranylgeranylation |
Miscellaneous databases
PROi | PR:P01120 |
RNActi | P01120, protein |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase IPR020849, Small_GTPase_Ras-type |
PANTHERi | PTHR24070, PTHR24070, 1 hit |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51421, RAS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RAS2_YEAST | |
Accessioni | P01120Primary (citable) accession number: P01120 Secondary accession number(s): D6W181, Q45U01 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 10, 2021 | |
This is version 222 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XIV
Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names - SIMILARITY comments
Index of protein domains and families