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Protein

Myc proto-oncogene protein

Gene

Myc

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-5689880 Ub-specific processing proteases
R-MMU-8866911 TFAP2 (AP-2) family regulates transcription of cell cycle factors
R-MMU-9018519 Estrogen-dependent gene expression

Names & Taxonomyi

Protein namesi
Recommended name:
Myc proto-oncogene protein
Alternative name(s):
Proto-oncogene c-Myc
Transcription factor p64
Gene namesi
Name:Myc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:97250 Myc

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Biotechnological usei

POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 are the four Yamanaka factors. When combined, these factors are sufficient to reprogram differentiated cells to an embryonic-like state designated iPS (induced pluripotent stem) cells. iPS cells exhibit the morphology and growth properties of ES cells and express ES cell marker genes.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi58T → A: Does not affect interaction with TRIM6. 1 Publication1
Mutagenesisi62S → A: Does not affect interaction with TRIM6. 1 Publication1
Mutagenesisi329S → A: Reduces phosphorylation by PIM2 by 60%, and decreases the transcriptional activity of MYC. 1 Publication1

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001272961 – 439Myc proto-oncogene proteinAdd BLAST439

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei8PhosphothreonineBy similarity1
Cross-linki52Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei58Phosphothreonine; by GSK3; alternateBy similarity1
Glycosylationi58O-linked (GlcNAc) threonine; alternateBy similarity1
Modified residuei62Phosphoserine; by DYRK2, GSK3 and CDK2By similarity1
Modified residuei71PhosphoserineBy similarity1
Modified residuei144N6-acetyllysine; by PCAF; alternateBy similarity1
Cross-linki144Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei149N6-acetyllysine; alternateCombined sources1
Cross-linki149Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei158N6-acetyllysine; by PCAFBy similarity1
Modified residuei162PhosphoserineBy similarity1
Modified residuei275N6-acetyllysine; by PCAFBy similarity1
Modified residuei293PhosphoserineBy similarity1
Cross-linki298Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei317N6-acetyllysine; by PCAFBy similarity1
Modified residuei323N6-acetyllysine; by PCAFBy similarity1
Modified residuei329Phosphoserine; by PIM2; in vitro1 Publication1
Modified residuei371N6-acetyllysine; by PCAFBy similarity1

Post-translational modificationi

Phosphorylated by PRKDC. Phosphorylated at Ser-62 by DYRK2; this primes the protein for subsequent phosphorylation by GSK3B at Thr-58. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome. Phosphorylation at Ser-62 by CDK2 prevents Ras-induced senescence (By similarity). Phosphorylation at Ser-329 by PIM2 leads to the stabilization of MYC.By similarity1 Publication
Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. Ubiquitination is counteracted by USP28 in the nucleoplasm and USP36 in the nucleolus, both interacting with of FBXW7, leading to its deubiquitination and preventing degradation. Also polyubiquitinated by the DCX(TRUSS) complex (By similarity). Ubiquitinated by TRIM6 in a phosphorylation-independent manner (PubMed:22328504).By similarity1 Publication

Keywords - PTMi

Acetylation, Glycoprotein, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP01108
MaxQBiP01108
PaxDbiP01108
PeptideAtlasiP01108
PRIDEiP01108

PTM databases

iPTMnetiP01108
PhosphoSitePlusiP01108

Expressioni

Developmental stagei

Expressed in the proliferating cells of the developing CNS and the epidermis. In the spinal cord at embryonic days 10.5, 11.5 and 12.5 dpc, expressed within a subset of cells in the proliferative ventricular zone, as well as in the differentiating cells at the ventral portion of the intermediate zone. Also detected in the roof plate and in the neural crest. At 14.5 dpc, found in regions containing differentiating postmitotic neurons. In the developing epidermis at 14.5 dpc, found in the dorsal lateral epidermis. At 17 dpc, expression is confined primarily to the proliferative malphigian layer of the epidermis and to the dermal papilla and primary germ cells in the dermis.1 Publication

Gene expression databases

BgeeiENSMUSG00000022346 Expressed in 280 organ(s), highest expression level in uterus
CleanExiMM_MYC
ExpressionAtlasiP01108 baseline and differential
GenevisibleiP01108 MM

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX (By similarity). Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts with KDM5A and KDM5B. Interacts (when phosphorylated at Thr-58 and Ser-62) with FBXW7. Interacts with PIM2 (PubMed:18438430). Interacts with RIOX1. The heterodimer MYC:MAX interacts with ABI1; the interaction may enhance MYC:MAX transcriptional activity (By similarity). Interacts with TRIM6 (PubMed:22328504). Interacts with NPM1; the binary complex is recruited to the promoter of MYC target genes and enhances their transcription (By similarity). Interacts with CIP2A; leading to the stabilization of MYC (By similarity).By similarity3 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201635, 15 interactors
ComplexPortaliCPX-1144 FOXO3-MYC complex
CPX-744 c-MYC-BIN1 complex
CPX-97 Transcriptional activator Myc-Max complex
CORUMiP01108
DIPiDIP-1064N
IntActiP01108, 17 interactors
STRINGi10090.ENSMUSP00000022971

Structurei

3D structure databases

ProteinModelPortaliP01108
SMRiP01108
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini354 – 406bHLHPROSITE-ProRule annotationAdd BLAST53

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni413 – 434Leucine-zipperAdd BLAST22

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi34 – 37Poly-Gln4
Compositional biasi89 – 92Poly-Gly4

Phylogenomic databases

eggNOGiENOG410IFSM Eukaryota
ENOG41124Q3 LUCA
GeneTreeiENSGT00510000046414
HOVERGENiHBG000472
InParanoidiP01108
KOiK04377
OMAiFANRNYD
PhylomeDBiP01108

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
IPR003327 Myc-LZ
IPR002418 Tscrpt_reg_Myc
IPR012682 Tscrpt_reg_Myc_N
PfamiView protein in Pfam
PF00010 HLH, 1 hit
PF02344 Myc-LZ, 1 hit
PF01056 Myc_N, 1 hit
PIRSFiPIRSF001705 Myc_protein, 1 hit
PRINTSiPR00044 LEUZIPPRMYC
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All

P01108-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPLNVNFTNR NYDLDYDSVQ PYFICDEEEN FYHQQQQSEL QPPAPSEDIW
60 70 80 90 100
KKFELLPTPP LSPSRRSGLC SPSYVAVATS FSPREDDDGG GGNFSTADQL
110 120 130 140 150
EMMTELLGGD MVNQSFICDP DDETFIKNII IQDCMWSGFS AAAKLVSEKL
160 170 180 190 200
ASYQAARKDS TSLSPARGHS VCSTSSLYLQ DLTAAASECI DPSVVFPYPL
210 220 230 240 250
NDSSSPKSCT SSDSTAFSPS SDSLLSSESS PRASPEPLVL HEETPPTTSS
260 270 280 290 300
DSEEEQEDEE EIDVVSVEKR QTPAKRSESG SSPSRGHSKP PHSPLVLKRC
310 320 330 340 350
HVSTHQHNYA APPSTRKDYP AAKRAKLDSG RVLKQISNNR KCSSPRSSDT
360 370 380 390 400
EENDKRRTHN VLERQRRNEL KRSFFALRDQ IPELENNEKA PKVVILKKAT
410 420 430
AYILSIQADE HKLTSEKDLL RKRREQLKHK LEQLRNSGA
Length:439
Mass (Da):48,971
Last modified:July 21, 1986 - v1
Checksum:i3FCA39BFFD6FC59E
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8WID3F8WID3_MOUSE
Myc proto-oncogene protein
Myc
454Annotation score:
B2RSN1B2RSN1_MOUSE
Myc proto-oncogene protein
Myc
454Annotation score:
A0A087WSQ0A0A087WSQ0_MOUSE
Myc proto-oncogene protein
Myc
453Annotation score:
F6PX41F6PX41_MOUSE
Myc proto-oncogene protein
Myc
453Annotation score:
E0CZD1E0CZD1_MOUSE
Myc proto-oncogene protein
Myc
32Annotation score:

Sequence cautioni

The sequence AAH06728 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti39E → D in K00683 (PubMed:6412145).Curated1
Sequence conflicti101E → Q in AAB59728 (PubMed:6321164).Curated1
Sequence conflicti284S → F in AAB59728 (PubMed:6321164).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L00039, L00038 Genomic DNA Translation: AAB59728.1
X01023 mRNA Translation: CAA25508.1
AK087961 mRNA Translation: BAC40060.1
AK133952 mRNA Translation: BAE21948.1
AK145084 mRNA Translation: BAE26228.1
BC006728 mRNA Translation: AAH06728.2 Different initiation.
K00683 Genomic DNA No translation available.
CCDSiCCDS49615.1
PIRiA93337 TVMS
RefSeqiNP_001170823.1, NM_001177352.1
NP_001170824.1, NM_001177353.1
NP_001170825.1, NM_001177354.1
UniGeneiMm.2444

Genome annotation databases

EnsembliENSMUST00000159327; ENSMUSP00000124758; ENSMUSG00000022346
ENSMUST00000160009; ENSMUSP00000123852; ENSMUSG00000022346
ENSMUST00000161976; ENSMUSP00000123821; ENSMUSG00000022346
GeneIDi17869
KEGGimmu:17869
UCSCiuc007vyh.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L00039, L00038 Genomic DNA Translation: AAB59728.1
X01023 mRNA Translation: CAA25508.1
AK087961 mRNA Translation: BAC40060.1
AK133952 mRNA Translation: BAE21948.1
AK145084 mRNA Translation: BAE26228.1
BC006728 mRNA Translation: AAH06728.2 Different initiation.
K00683 Genomic DNA No translation available.
CCDSiCCDS49615.1
PIRiA93337 TVMS
RefSeqiNP_001170823.1, NM_001177352.1
NP_001170824.1, NM_001177353.1
NP_001170825.1, NM_001177354.1
UniGeneiMm.2444

3D structure databases

ProteinModelPortaliP01108
SMRiP01108
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201635, 15 interactors
ComplexPortaliCPX-1144 FOXO3-MYC complex
CPX-744 c-MYC-BIN1 complex
CPX-97 Transcriptional activator Myc-Max complex
CORUMiP01108
DIPiDIP-1064N
IntActiP01108, 17 interactors
STRINGi10090.ENSMUSP00000022971

PTM databases

iPTMnetiP01108
PhosphoSitePlusiP01108

Proteomic databases

EPDiP01108
MaxQBiP01108
PaxDbiP01108
PeptideAtlasiP01108
PRIDEiP01108

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000159327; ENSMUSP00000124758; ENSMUSG00000022346
ENSMUST00000160009; ENSMUSP00000123852; ENSMUSG00000022346
ENSMUST00000161976; ENSMUSP00000123821; ENSMUSG00000022346
GeneIDi17869
KEGGimmu:17869
UCSCiuc007vyh.2 mouse

Organism-specific databases

CTDi4609
MGIiMGI:97250 Myc

Phylogenomic databases

eggNOGiENOG410IFSM Eukaryota
ENOG41124Q3 LUCA
GeneTreeiENSGT00510000046414
HOVERGENiHBG000472
InParanoidiP01108
KOiK04377
OMAiFANRNYD
PhylomeDBiP01108

Enzyme and pathway databases

ReactomeiR-MMU-5689880 Ub-specific processing proteases
R-MMU-8866911 TFAP2 (AP-2) family regulates transcription of cell cycle factors
R-MMU-9018519 Estrogen-dependent gene expression

Miscellaneous databases

ChiTaRSiMyc mouse
PROiPR:P01108
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022346 Expressed in 280 organ(s), highest expression level in uterus
CleanExiMM_MYC
ExpressionAtlasiP01108 baseline and differential
GenevisibleiP01108 MM

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
IPR003327 Myc-LZ
IPR002418 Tscrpt_reg_Myc
IPR012682 Tscrpt_reg_Myc_N
PfamiView protein in Pfam
PF00010 HLH, 1 hit
PF02344 Myc-LZ, 1 hit
PF01056 Myc_N, 1 hit
PIRSFiPIRSF001705 Myc_protein, 1 hit
PRINTSiPR00044 LEUZIPPRMYC
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMYC_MOUSE
AccessioniPrimary (citable) accession number: P01108
Secondary accession number(s): P70247, Q3UM70, Q61422
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 7, 2018
This is version 196 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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