UniProtKB - P01101 (FOS_MOUSE)
Protein c-Fos
Fos
Functioni
Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex, at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling (By similarity).
Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.
By similarity5 PublicationsGO - Molecular functioni
- chromatin binding Source: MGI
- DNA binding Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- double-stranded DNA binding Source: MGI
- identical protein binding Source: MGI
- protein-containing complex binding Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
- RNA polymerase II core promoter sequence-specific DNA binding Source: MGI
- RNA polymerase II-specific DNA-binding transcription factor binding Source: MGI
- R-SMAD binding Source: MGI
- sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
- transcription cis-regulatory region binding Source: MGI
- transcription coregulator binding Source: Ensembl
GO - Biological processi
- aging Source: Ensembl
- cellular response to cadmium ion Source: MGI
- cellular response to calcium ion Source: MGI
- cellular response to extracellular stimulus Source: MGI
- cellular response to hormone stimulus Source: Ensembl
- cellular response to reactive oxygen species Source: MGI
- conditioned taste aversion Source: Ensembl
- female pregnancy Source: Ensembl
- nervous system development Source: MGI
- positive regulation of neuron death Source: MGI
- positive regulation of osteoclast differentiation Source: MGI
- positive regulation of pri-miRNA transcription by RNA polymerase II Source: MGI
- positive regulation of transcription, DNA-templated Source: MGI
- positive regulation of transcription by RNA polymerase II Source: NTNU_SB
- regulation of gene expression Source: MGI
- regulation of transcription, DNA-templated Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
- response to cAMP Source: Ensembl
- response to cold Source: Ensembl
- response to corticosterone Source: Ensembl
- response to cytokine Source: Ensembl
- response to gravity Source: Ensembl
- response to immobilization stress Source: Ensembl
- response to light stimulus Source: Ensembl
- response to lipopolysaccharide Source: Ensembl
- response to muscle stretch Source: MGI
- response to progesterone Source: Ensembl
- response to toxic substance Source: Ensembl
- response to xenobiotic stimulus Source: MGI
- skeletal muscle cell differentiation Source: MGI
- sleep Source: Ensembl
- SMAD protein signal transduction Source: MGI
- transcription by RNA polymerase II Source: MGI
- transforming growth factor beta receptor signaling pathway Source: MGI
Keywordsi
Molecular function | DNA-binding |
Enzyme and pathway databases
Reactomei | R-MMU-2559580, Oxidative Stress Induced Senescence R-MMU-2871796, FCERI mediated MAPK activation R-MMU-450341, Activation of the AP-1 family of transcription factors R-MMU-9018519, Estrogen-dependent gene expression |
Names & Taxonomyi
Protein namesi | Recommended name: Protein c-FosCuratedAlternative name(s): Cellular oncogene fos FBJ osteosarcoma oncogeneImported Transcription factor AP-1 subunit c-FosCurated |
Gene namesi | Name:Fos |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:95574, Fos |
VEuPathDBi | HostDB:ENSMUSG00000021250 |
Subcellular locationi
Cytoplasm and Cytosol
- cytosol By similarity
Endoplasmic reticulum
- Endoplasmic reticulum By similarity
Nucleus
- Nucleus PROSITE-ProRule annotation
Note: In quiescent cells, present in very small amounts in the cytosol. Following induction of cell growth, first localizes to the endoplasmic reticulum and only later to the nucleus. Localization at the endoplasmic reticulum requires dephosphorylation at Tyr-10 and Tyr-30 (By similarity).By similarity
Cytosol
- cytosol Source: UniProtKB-SubCell
Endoplasmic reticulum
- endoplasmic reticulum Source: CACAO
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
- transcription factor AP-1 complex Source: ComplexPortal
Other locations
- cytoplasm Source: CACAO
- membrane Source: MGI
- neuron projection Source: MGI
- protein-DNA complex Source: MGI
- transcription regulator complex Source: MGI
Keywords - Cellular componenti
Cytoplasm, Endoplasmic reticulum, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 139 | K → N: No effect on activation of phospholipid synthesis. 1 Publication | 1 | |
Mutagenesisi | 144 | R → N: No effect on activation of phospholipid synthesis, nor on CDS1-binding. 1 Publication | 1 | |
Mutagenesisi | 146 | R → N: Complete loss of activation of phospholipid synthesis. No effect on CDS1-binding. 1 Publication | 1 | |
Mutagenesisi | 165 – 193 | LQAET…EKEKL → AQAETDQAEDEKSAAQTEIA NAAKEKEKA: Disrupts interaction with SMARCB1, SMARCD1, ARID1A and JUN. 1 PublicationAdd BLAST | 29 | |
Mutagenesisi | 232 | T → A: No effect on PDGF-stimulated enhancement of transcriptional activity. Completely abolishes PDGF-stimulated enhancement of transcriptional activity; when associated with A-325; A-331 and A-374. 1 Publication | 1 | |
Mutagenesisi | 325 | T → A: Almost no EGF-mediated phosphorylation, greatly reduced cellular transformation, and reduced AP1 activity by 20%; when associated with A-331. No effect on PDGF-stimulated enhancement of transcriptional activity. Completely abolishes PDGF-stimulated enhancement of transcriptional activity; when associated with A-232; A-331 and A-374. 2 Publications | 1 | |
Mutagenesisi | 331 | T → A: Almost no EGF-mediated phosphorylation, greatly reduced cellular transformation, and reduced AP1 activity by 20%; when associated with A-325. No effect on PDGF-stimulated enhancement of transcriptional activity. Completely abolishes PDGF-stimulated enhancement of transcriptional activity; when associated with A-232; A-325;and A-374. 2 Publications | 1 | |
Mutagenesisi | 343 | F → A: Reduced phosphorylation by ERK. Reduced AP1 activity by 65%. 1 Publication | 1 | |
Mutagenesisi | 345 | Y → A: Reduced phosphorylation by ERK. 1 Publication | 1 | |
Mutagenesisi | 362 | S → D: Enhanced EGF- and RSK-mediated transformation; when associated with D-374. 1 Publication | 1 | |
Mutagenesisi | 362 | S → E: Increased enhancement of EGF- and RSK-mediated transformation; when associated with E-374. 1 Publication | 1 | |
Mutagenesisi | 374 | S → A: No effect on PDGF-stimulated enhancement of transcriptional activity. Completely abolishes PDGF-stimulated enhancement of transcriptional activity; when associated with A-232; A-325 and A-331. 2 Publications | 1 | |
Mutagenesisi | 374 | S → D: Enhanced EGF- and RSK-mediated transformation; when associated with D-362. 2 Publications | 1 | |
Mutagenesisi | 374 | S → E: Enhanced EGF- and RSK-mediated transformation; when associated with E-362. 2 Publications | 1 |
Keywords - Diseasei
Proto-oncogeneChemistry databases
ChEMBLi | CHEMBL4105918 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000076467 | 1 – 380 | Protein c-FosAdd BLAST | 380 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 10 | Phosphotyrosine; by SRCBy similarity | 1 | |
Modified residuei | 30 | Phosphotyrosine; by SRCBy similarity | 1 | |
Cross-linki | 113 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 128 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 232 | Phosphothreonine1 Publication | 1 | |
Cross-linki | 265 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity | ||
Cross-linki | 265 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 325 | Phosphothreonine; by MAPK1 and MAPK32 Publications | 1 | |
Modified residuei | 331 | Phosphothreonine; by MAPK1 and MAPK32 Publications | 1 | |
Modified residuei | 362 | Phosphoserine; by MAPK1, MAPK3 and RPS6KA33 Publications | 1 | |
Modified residuei | 374 | Phosphoserine; by MAPK1 and MAPK32 Publications | 1 |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
MaxQBi | P01101 |
PaxDbi | P01101 |
PRIDEi | P01101 |
ProteomicsDBi | 267395 |
PTM databases
iPTMneti | P01101 |
PhosphoSitePlusi | P01101 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000021250, Expressed in granulocyte and 299 other tissues |
Genevisiblei | P01101, MM |
Interactioni
Subunit structurei
Heterodimer; with JUN (PubMed:29272704).
Component of the SMAD3/SMAD4/JUN/FOS complex required for synergistic TGF-beta-mediated transcription at the AP1 promoter site (By similarity).
Interacts with SMAD3; the interaction is weak even on TGF-beta activation (By similarity).
Interacts with MAFB (By similarity).
Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA (PubMed:11397794).
Interacts with CDS1 and PI4K2A, but not with CDIPT, nor PI4K2B (PubMed:22105363).
Interacts (via bZIP domain and leucine-zipper region) with the multiprotein chromatin-remodeling complexes SWI/SNF: SWI/SNF-A (BAF) subunits SMARCB1, SMARCC2 and SMARCD1 (PubMed:29272704).
Interacts (via bZIP domain and leucine-zipper region) with ARID1A (PubMed:29272704).
By similarity3 PublicationsBinary interactionsi
P01101
With | #Exp. | IntAct |
---|---|---|
Esr2 [O08537] | 2 | EBI-4288185,EBI-2526214 |
Mafb [P54841] | 4 | EBI-4288185,EBI-16093217 |
Mecom [P14404] | 2 | EBI-4288185,EBI-1994523 |
GO - Molecular functioni
- identical protein binding Source: MGI
- RNA polymerase II-specific DNA-binding transcription factor binding Source: MGI
- R-SMAD binding Source: MGI
- transcription coregulator binding Source: Ensembl
Protein-protein interaction databases
BioGRIDi | 199726, 40 interactors |
ComplexPortali | CPX-610, AP-1 transcription factor complex FOS-JUN-NFATC2 CPX-611, bZIP transcription factor complex, Fos-Jun |
DIPi | DIP-1066N |
ELMi | P01101 |
IntActi | P01101, 4 interactors |
MINTi | P01101 |
STRINGi | 10090.ENSMUSP00000021674 |
Miscellaneous databases
RNActi | P01101, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P01101 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 137 – 200 | bZIPPROSITE-ProRule annotationAdd BLAST | 64 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 117 – 141 | DisorderedSequence analysisAdd BLAST | 25 | |
Regioni | 139 – 159 | Basic motif; required for the activation of phospholipid synthesis, but not for CDS1-bindingAdd BLAST | 21 | |
Regioni | 165 – 193 | Leucine-zipperPROSITE-ProRule annotationAdd BLAST | 29 | |
Regioni | 354 – 380 | DisorderedSequence analysisAdd BLAST | 27 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 357 – 380 | Polar residuesSequence analysisAdd BLAST | 24 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1414, Eukaryota |
GeneTreei | ENSGT00940000159276 |
HOGENOMi | CLU_049742_2_0_1 |
InParanoidi | P01101 |
OMAi | FTYPEAE |
OrthoDBi | 1221590at2759 |
PhylomeDBi | P01101 |
TreeFami | TF326301 |
Family and domain databases
IDEALi | IID50165 |
InterProi | View protein in InterPro IPR000837, AP-1 IPR004827, bZIP IPR029816, c-Fos/v-Fos |
PANTHERi | PTHR23351, PTHR23351, 1 hit PTHR23351:SF4, PTHR23351:SF4, 1 hit |
Pfami | View protein in Pfam PF00170, bZIP_1, 1 hit |
PRINTSi | PR00042, LEUZIPPRFOS |
SMARTi | View protein in SMART SM00338, BRLZ, 1 hit |
PROSITEi | View protein in PROSITE PS50217, BZIP, 1 hit PS00036, BZIP_BASIC, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MMFSGFNADY EASSSRCSSA SPAGDSLSYY HSPADSFSSM GSPVNTQDFC
60 70 80 90 100
ADLSVSSANF IPTVTAISTS PDLQWLVQPT LVSSVAPSQT RAPHPYGLPT
110 120 130 140 150
QSAGAYARAG MVKTVSGGRA QSIGRRGKVE QLSPEEEEKR RIRRERNKMA
160 170 180 190 200
AAKCRNRRRE LTDTLQAETD QLEDEKSALQ TEIANLLKEK EKLEFILAAH
210 220 230 240 250
RPACKIPDDL GFPEEMSVAS LDLTGGLPEA STPESEEAFT LPLLNDPEPK
260 270 280 290 300
PSLEPVKSIS NVELKAEPFD DFLFPASSRP SGSETSRSVP DVDLSGSFYA
310 320 330 340 350
ADWEPLHSNS LGMGPMVTEL EPLCTPVVTC TPGCTTYTSS FVFTYPEADS
360 370 380
FPSCAAAHRK GSSSNEPSSD SLSSPTLLAL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V00727 Genomic DNA Translation: CAA24105.1 J00370 Genomic DNA Translation: AAA96699.1 BC029814 mRNA Translation: AAH29814.1 |
CCDSi | CCDS26059.1 |
PIRi | A01343, TVMSF |
RefSeqi | NP_034364.1, NM_010234.2 |
Genome annotation databases
Ensembli | ENSMUST00000021674; ENSMUSP00000021674; ENSMUSG00000021250 |
GeneIDi | 14281 |
KEGGi | mmu:14281 |
UCSCi | uc007oha.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V00727 Genomic DNA Translation: CAA24105.1 J00370 Genomic DNA Translation: AAA96699.1 BC029814 mRNA Translation: AAH29814.1 |
CCDSi | CCDS26059.1 |
PIRi | A01343, TVMSF |
RefSeqi | NP_034364.1, NM_010234.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2WT7 | X-ray | 2.30 | A | 138-200 | [»] | |
SMRi | P01101 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 199726, 40 interactors |
ComplexPortali | CPX-610, AP-1 transcription factor complex FOS-JUN-NFATC2 CPX-611, bZIP transcription factor complex, Fos-Jun |
DIPi | DIP-1066N |
ELMi | P01101 |
IntActi | P01101, 4 interactors |
MINTi | P01101 |
STRINGi | 10090.ENSMUSP00000021674 |
Chemistry databases
ChEMBLi | CHEMBL4105918 |
PTM databases
iPTMneti | P01101 |
PhosphoSitePlusi | P01101 |
Proteomic databases
MaxQBi | P01101 |
PaxDbi | P01101 |
PRIDEi | P01101 |
ProteomicsDBi | 267395 |
Protocols and materials databases
ABCDi | P01101, 3 sequenced antibodies |
Antibodypediai | 4375, 1906 antibodies from 51 providers |
DNASUi | 14281 |
Genome annotation databases
Ensembli | ENSMUST00000021674; ENSMUSP00000021674; ENSMUSG00000021250 |
GeneIDi | 14281 |
KEGGi | mmu:14281 |
UCSCi | uc007oha.2, mouse |
Organism-specific databases
CTDi | 2353 |
MGIi | MGI:95574, Fos |
VEuPathDBi | HostDB:ENSMUSG00000021250 |
Phylogenomic databases
eggNOGi | KOG1414, Eukaryota |
GeneTreei | ENSGT00940000159276 |
HOGENOMi | CLU_049742_2_0_1 |
InParanoidi | P01101 |
OMAi | FTYPEAE |
OrthoDBi | 1221590at2759 |
PhylomeDBi | P01101 |
TreeFami | TF326301 |
Enzyme and pathway databases
Reactomei | R-MMU-2559580, Oxidative Stress Induced Senescence R-MMU-2871796, FCERI mediated MAPK activation R-MMU-450341, Activation of the AP-1 family of transcription factors R-MMU-9018519, Estrogen-dependent gene expression |
Miscellaneous databases
BioGRID-ORCSi | 14281, 1 hit in 67 CRISPR screens |
ChiTaRSi | Fos, mouse |
PROi | PR:P01101 |
RNActi | P01101, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000021250, Expressed in granulocyte and 299 other tissues |
Genevisiblei | P01101, MM |
Family and domain databases
IDEALi | IID50165 |
InterProi | View protein in InterPro IPR000837, AP-1 IPR004827, bZIP IPR029816, c-Fos/v-Fos |
PANTHERi | PTHR23351, PTHR23351, 1 hit PTHR23351:SF4, PTHR23351:SF4, 1 hit |
Pfami | View protein in Pfam PF00170, bZIP_1, 1 hit |
PRINTSi | PR00042, LEUZIPPRFOS |
SMARTi | View protein in SMART SM00338, BRLZ, 1 hit |
PROSITEi | View protein in PROSITE PS50217, BZIP, 1 hit PS00036, BZIP_BASIC, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FOS_MOUSE | |
Accessioni | P01101Primary (citable) accession number: P01101 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | February 23, 2022 | |
This is version 184 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families